in cell nmr in mammalian cells part 2

... S, Han SI (eds) NMR imaging in chemical engineering, Wiley-VCH, Weinheim, pp 25 0 26 3 24 Stapf S, Han S (20 05) NMR imaging in chemical engineering Wiley-VCH, Weinheim 25 Wolf F, Hecht L, Schuchmann ... Verfahrenstechnik und Mechanik Adenauerring 20 b 76131 Karlsruhe Germany Edme.Hardy@kit.edu ISBN 978-3-6 42- 21 627 -5 e-ISBN 978-3-6 42- 21 628 -2 DOI 10.1007/978-3-6 42- 21 628 -2 Springer Heidelberg Dordrecht London ... the induction constant : B D H E.H Hardy, NMR Methods for the Investigation of Structure and Transport, DOI 10.1007/978-3-6 42- 21 628 -2 2, © Springer-Verlag Berlin Heidelberg 20 12 Fundamentals In...

Ngày tải lên: 29/05/2014, 18:32

... counselling after the diagnosis 12 In my opinion working according the guideline requires that the content of the preoperative counselling is put in writing 13 In my opinion working according the ... guideline Time investment* RI 12 8500 (1) 10 28 77 (2) 58 32 (3) 0656 (4) 4579 (5) 80 02 (6) 5743 (7) 1716 (8) 8736 (9) 1813 (10) 023 4 (11) 8478 ( 12) 4884 (13) 3883 (14) 1348 (15) 29 89 (16) 027 1 (17) ... guideline I expect that colleagues cooperate in applying the guideline I expect that management cooperates in applying the guideline I expect that ward nursing staff cooperates in applying the...

Ngày tải lên: 11/08/2014, 05:21

... counselling after the diagnosis 12 In my opinion working according the guideline requires that the content of the preoperative counselling is put in writing 13 In my opinion working according the ... guideline Time investment* RI 12 8500 (1) 10 28 77 (2) 58 32 (3) 0656 (4) 4579 (5) 80 02 (6) 5743 (7) 1716 (8) 8736 (9) 1813 (10) 023 4 (11) 8478 ( 12) 4884 (13) 3883 (14) 1348 (15) 29 89 (16) 027 1 (17) ... guideline I expect that colleagues cooperate in applying the guideline I expect that management cooperates in applying the guideline I expect that ward nursing staff cooperates in applying the...

Ngày tải lên: 11/08/2014, 16:21

... struc- NMR structure of micelle-associated HIV-1 VpUcyt 23 24 25 26 27 28 29 30 31 32 33 ture of the hydrophilic region of HIV-1 encoded virus protein U (Vpu) by CD and 1H NMR spectroscopy Int J ... Samples for selected NMR experiments in H2O-free buffer were prepared in NMR buffer, lyophilized and redissolved in 2H2O (99.99% 2H, Sigma-Aldrich, FEBS Journal 27 6 (20 09) 6560–6575 ª 20 09 The Authors ... protein is cell type independent and FEBS Journal 27 6 (20 09) 6560–6575 ª 20 09 The Authors Journal compilation ª 20 09 FEBS M Wittlich et al 10 11 12 13 14 15 16 17 18 19 20 21 22 occurs in cultured...

Ngày tải lên: 18/02/2014, 06:20

... 19 20 21 22 23 24 25 26 27 28 29 30 masses greater than 20 0 kilodaltons Science 314, 109– 1 12 Aebersold R & Mann M (20 03) Mass spectrometrybased proteomics Nature 422 , 198 20 7 Yates JR 3rd (20 04) ... characterize ATP-binding and GTP-binding proteins in cells, we profiled purine-binding proteins on a proteome scale [40] For this purpose, we used 5¢-p-fluorosulfonylbenzoyladenosine (FSBA) (Fig ... with the 2D pattern of silver-stained proteins pointed to candidate ATP-binding or GTPbinding proteins that were selected from the 2D gel and identified by MS In this way, 12 different proteins could...

Ngày tải lên: 18/02/2014, 16:20

... 1998, 75, 6 62 22 Cieplak, M.; Hoang, T X.; Robbins, M O Proteins: Struct Funct Genet 20 02, 49, 114 23 Cieplak, M.; Hoang, T X.; Robbins, M O Proteins: Struct Funct Bioinfo 20 04, 56, 28 5 24 Eom, K.; ... Angew Chem Int Ed 20 07, 46, 22 32 17 Liphardt, J.; Dumont, S.; Smith, S B.; Tinoco, I.; Bustamante, C Science 20 02, 29 6, 18 32 18 Collin, D.; Ritort, F.; Jarzynski, C.; Smith, S B.; Tinoco, I.; ... unfolding mechanism .23 Eom et al24 ,25 provided the coarse-grained model of folded polymer chain molecules for gaining insight into unfolding mechanism with respect to folding topology, and it...

Ngày tải lên: 22/02/2014, 08:20

... & Nakatani, Y (20 00) 578 A Bauer and B Kuster (Eur J Biochem 27 0) 20 21 22 23 24 25 26 27 Involvement of the TIP60 histone acetylase complex in DNA repair and apoptosis Cell 1 02, 463–473 Becamel, ... protein J Mol Biol 314, 120 9– 122 5 15 Jackson, V (1999) Formaldehyde cross-linking for studying nucleosomal dynamics Methods 17, 125 –139 16 Fancy, D.A (20 00) Elucidation of protein–protein interactions ... Demey, E., Jouin, P., Ullmer, C., Dumuis, A., Bockaert, J & Marin, P (20 02) Synaptic multiprotein complexes associated with 5-HT (2C) receptors: a proteomic approach EMBO J 21 , 23 32 23 42 Rappsilber,...

Ngày tải lên: 31/03/2014, 07:20

... cultured in RPMI 1640 containing mmol/L glutamine and supplemented with 20 % fetal calf serum (FCS) at 37°C in air containing 5% CO2 Cells were harvested by incubation with phosphate-buffered saline ... Biosciences) by incubating the cells in 100 μL PBS for 10’ at 4°C Cells were then washed with PBS, and fixed with 1% formaldehyde for 20 ’ at 4°C before permeabilization for intracellular staining To permeabilize ... Curve The cell line SW 620 was employed to obtain a calibration curve for the CD45-depletion method according to the following procedure: cells were harvested by incubating with PBS containing mM...

Ngày tải lên: 18/06/2014, 19:20

... cultured in RPMI 1640 containing mmol/L glutamine and supplemented with 20 % fetal calf serum (FCS) at 37°C in air containing 5% CO2 Cells were harvested by incubation with phosphate-buffered saline ... Biosciences) by incubating the cells in 100 μL PBS for 10’ at 4°C Cells were then washed with PBS, and fixed with 1% formaldehyde for 20 ’ at 4°C before permeabilization for intracellular staining To permeabilize ... Curve The cell line SW 620 was employed to obtain a calibration curve for the CD45-depletion method according to the following procedure: cells were harvested by incubating with PBS containing mM...

Ngày tải lên: 20/06/2014, 03:20

... bioconjugating QD-COOH to proteins of increasingly higher pI, using cationic proteins such as cytochrome c and lysozyme However, it was observed that the pI of proteins can play a determining factor in ... and cellular imaging with quantum dots Analyst 20 04, 129 :6 72- 677 Medintz IL, Uyeda HT, Goldman ER, Mattoussi H, et al.: Quantum dot bioconjugates for imaging, labelling and sensing Nat Mater 20 05, ... 28 (15):1094 -20 09 Baryla NE, Lucy CA: Simultaneous separation of cationic and anionic proteins using zwitterionic surfactants in capillary electrophoresis Anal Chem 20 00, 72: 228 0 -22 84 Wei W, Ju...

Ngày tải lên: 11/08/2014, 00:22

... manuscript CW, MRu, NCG have been involved in drafting the manuscript NCG was involved in revising the manuscript All authors read and approved the final manuscript 22 Kucisec-Tepes N, Bejuk D, Kosuta ... for the characterization of infection risk in gunshot injuries:Technology, principal consideration and clinical implementation Head & Face Medicine 20 11 7:18 Competing interests The authors declare ... declare that they have no competing interests Received: 26 September 20 11 Accepted: 27 October 20 11 Published: 27 October 20 11 References Fackler ML: Ballistic injury Ann Emerg Med 1986, 15:1451-5...

Ngày tải lên: 11/08/2014, 20:21

... 54301 25 4861 58001 57701 1 320 1 14001 7701 25 4847 25 4846 25 48 62 254848 25 4867 7401 25 4868 25 4853 25 4858 25 4850 25 4864 25 425 5 25 4865 25 4866 25 4849 25 4857 25 4856 25 48 52 254859 25 4863 25 4851 25 4844 25 4860 ... 1 52 4 026 25 4865 25 4850 5 620 1 15701 1301 10501 25 4858 329 803 7701 183 3984 92 701 1 02 4467 113 99 327 4 72 57701 3091 4169 3979 25 4857 25 4868 25 4867 25 48 52 254860 25 4851 25 4844 25 4863 7401 25 4866 ... (27 7 -29 6) (Cit 27 8, 29 2) Biglycan (24 7 -26 6) HSP60 (28 7 -29 7) Fibromodulin (20 1 -22 0) Acetyl-calpastatin (184 -21 0) Osteoglycin (176-195) Serine Protease II (433-4 52) Histone 2A (95-114) Histone 2B/e...

Ngày tải lên: 12/08/2014, 17:22

... (ENAMINE– T56 625 23), B11– (ENAMINE– T5655673), B13– (ENAMINE– T 624 5968), B15– (ENAMINE– T 624 1917), B16– (ENAMINE– T6053 724 ), B17– (ENAMINE– T60367 72) , B18– (ENAMINE– T60 320 83), B19– (ENAMINE– T 626 6966), ... Figure 23 : Two dimensional maps showing NAD+ binding with ALDH3A1 79 Figure 24 A: Density map showing the binding of CB25 with ALDH3A1 .83 Figure 24 B: Two dimensional map showing CB25 binding ... of CB29 binding .113 B Selectivity of CB29 for ALDH3A1 versus ALDH1A1 and ALDH2 117 C Characterization of CB7 binding 123 D Probing CB7 binding of ALDH3A1 site using Q 122 A and...

Ngày tải lên: 24/08/2014, 09:51

... There are very few joints 22 2R 22 4R 3 62. 1R 423 .3R 504R 515.1R Corrosion of Metals in Concrete Control of Cracking in Concrete Structures Guide for the Design of Durable Parking Structures Recommendations ... required Inspect deck drain function M S a Deck drains: remove debris and clean out drain; b Drain lines: check for leaks and damage; c Inspect joint sealant at top of drain AR A Install area drain ... information regarding concrete durability Testing may include compressive strength, chain dragging, and half -cell testing to locate GUIDE FOR STRUCTURAL MAINTENANCE OF PARKING STRUCTURES 3 62. 2R-3 active...

Ngày tải lên: 24/10/2014, 21:59

Ngày tải lên: 14/11/2014, 06:20

... 18 2. 1 Working Principle of Piezoresponse Force Microscopy 18 2. 1.1 Experimental Setup 2. 2 Theory of PFM 18 21 2. 2.1 Capacitive forces 23 2. 2 .2 Electromechanical Forces 25 2. 3 Domain Switching ... A, 20 1 (20 04) 497 [20 ] K T Kim and C L Kim, J Eur Ceram Soc., 24 (20 04) 26 13 [21 ] N Shigemitsu, Jpn J Appl Phys., 43 (20 04) 6613 [22 ] D Kim, D Klingensmith, D Dalton, Integr Ferroelectr., 68 (20 04) ... capturing Molotskii et al [26 , 27 ] proposed a closed-form solution for domain switching in the point charge approximation Kalinin et al [28 ] modified the thermodynamics of domain switching, using...

Ngày tải lên: 10/09/2015, 08:41

... Intrinsically Disorder Protein 25 1 .2. 4.1 Bioinformatics analysis 1 .2. 4 .2 Experimental methods 1.3 25 26 Protein Structure Determination by NMR 1.3.1 Unique Features of NMR 29 29 III 1.3 .2 NMR Phenomenon ... Structure–Function Paradigm 23 1 .2. 2 Characterizations of Intrinsically Disordered Proteins 24 1 .2. 3 Biological Function of Intrinsically Disordered Proteins 25 1 .2. 4 Methods to Characterize Intrinsically Disorder ... E.coli Cells 45 2. 3 Transformation of E coli Cells 46 2. 4 Protein Expression and Purification 46 2. 5 Preparation of Isotope Labeled Proteins 47 2. 6 Protein Analysis by SDS-PAGE 47 2. 7 Determination...

Ngày tải lên: 14/09/2015, 12:53

... connecting and protecting outgoing line powering pairs in  ONU (optical network unit) host cabinets • DLC remote terminal OSP pair applications Compact solution for all OSP pairs leaving DLC cabinets ... +1-9 52- 938-8080 • 1-800-366-3891 Ordering Information Description Catalog Number ComProtect® protectors with ground bars Package of 10, black housing 5909 21 2-00 Package of 8, black housing 5909 21 1-00 ... operation ( 36.5 mm) (2. 64mm) ( 9.4 mm) ( 20 .613 mm) ( 8.7 mm) ( 11.4 mm) (17.8mm) ComProtect dimensions ComProtect unit inserted into Series Block (21 .33mm) (61.9mm) (2. 64mm) • ons Single-Pair Protectors...

Ngày tải lên: 10/12/2013, 18:15

... for arriving at the expansion of state functions in different bases such as the form (2. 28) and (2. 29) or (2. 28a) and (2. 29a) For example, applying the unit operators (2. 41) and (2. 42) to an ... of (2. 25) r by Yqi ð~0 Þ followed by summing over qi, it can be shown that the completeness relation r (2. 26) follows from the orthogonality condition (2. 25) Substituting (2. 26) into (2. 27) gives: ... the particles in the final state in the ensemble is, however, proportional to jYj2 It contains not only the terms ja1 j2 jY1 j2 þ ja2 j2 jY2 j2 but also the cross terms, or the ‘‘interference...

Ngày tải lên: 17/03/2014, 14:41