improving lysine and threonine accumulation via transfer of bacterial genes

... Influence of the phenylalanine and elicitors on cell growth and taxol accumulation 76 Table 4.9 Effect of adding time of elicitor on taxol accumulation 81 Table 4.10 Effect of exposure ... objective of in vitro production of paclitaxel, the specific objectives of this research were i) to investigate medium components for optimizing cell growth and paclitaxel accumulation via callus and ... limitations of Taxus sp are very slow growing and the low content of paclitaxel (0.01% dry weight of bark) For example, the commercial isolation of kg of Taxol required about 6.7 tons of Taxus brevifolia...

Ngày tải lên: 23/10/2015, 15:38

... perturbation of the luminal side of PSI involving PSI-F and PSI-N The absence of PSI-J might affect the conformation of PSI-F, which, in turn, changes the binding of PSI-N PSI-F provides part of the ... the luminal side of PSI is perturbed, probably because Knock-out of the J subunit of PSI of destabilization of PSI-F in the absence of PSI-J, resulting in an increased pool of inactive PSI Experimental ... corresponding to 1.0, 0.5, and 0.25 lg Chl of the wild-type and 1.0–0.5 lg Chl of the mutant was separated by SDS ⁄ PAGE, blotted and analyzed with the antibodies indicated Wild-type (WT) and DpsaJ dilutions...

Ngày tải lên: 16/03/2014, 11:20

... (DMF), tetrahydrofuran (THF), ethanol, hydrofluoric acid, and other solvents used were all of analytical reagent grade and obtained from Tianjin Chemical Co., Tianjin, China, and were used without ... etching of the silica templates, the FTIR technique was used In the FTIR spectrum of the products treated with HF, the absorption bands at 1105 cm-1 of the Si–O–Si symmetric stretching mode and dSi-O ... Fig 3a and b The collapse of the crosslinked polymeric shells during the etching in DMF maybe due to the lower crosslinking degree [29] and the osmotic pressure between the inner and outer of the...

Ngày tải lên: 22/06/2014, 00:20

... between knowledge and successful knowledge transfer CONCLUSION The purpose of this paper is to increase the understanding of how knowledge transfers can be perceived and managed, and how choices ... the plants in terms of transfer success or failure Plants and failed to take on the knowledge transfer, whereas the other four succeeded Transfer of Knowledge and the Role of Motivation CASE STUDY ... RK 1994 Case study research: Design and methods (2nd edn) Sage: Thousand Oaks, CA Zander U, Kogut B 1995 Knowledge and the speed of transfer and imitation of organizational capabilities An empirical...

Ngày tải lên: 24/01/2014, 00:20

... levels [9] and the effect of a maintenance dosing of CsA on the recovery of serum Gb3 levels after termination of ERT was determined ERT is an effective means of eliminating serum Gb3, and Gb3 remained ... and the effect of CsA on ‘relapse’ of Gb3 accumulation monitored ERT primarily affects serum and liver Gb3 accumulation [9] and these tissues were therefore the primary focus of our study, although ... Gb3 synthesis in this model and have shown that the inhibition of MDR1 is a viable potential approach to the reduction of Gb3 in both the serum and certain tissues of this model Results MDR1 inhibition...

Ngày tải lên: 19/02/2014, 07:20

... coordinated changes in the expression of the transferrin receptor and ferritin; (b) developed iron accumulation and (c) increased the prooxidant state and the level of oxidatively modified proteins ... consumption and to examine the possible role played by cells and molecules of the immunological system in hepatic injury and repair Understanding the interdependence between the metabolism of iron and ... Igs) and Super-Signal West Femto (Perbio) Densitometry quantification of the CD1d protein bands indicated was performed using a Kodak Digital Science DC40 camera and its associated software Band...

Ngày tải lên: 19/02/2014, 16:20

... input variables and to explain the behaviour of the branch-point in terms of the kinetic properties of CGS and TS Sensitivity analysis In a rst analysis, uxes of cystathionine and threonine (Fig ... independent of what happens on the cystathionine side The Phser branch-point combines the divergence of two uxes (uxes of cystathionine and threonine) with the convergence of two uxes (uxes of Phser and ... steady-state, the ux of Phser (JPhser) is equal to the sum of the ux of cystathionine (Jcystathionine) and the ux of threonine (JThr) (Eqn 10) JPhser ẳ Jcystathionine ỵ JThr 10ị Jcystathionine and JThr in...

Ngày tải lên: 20/02/2014, 02:21

... strand B6 close to the dyad axis between monomers A and C (or B and D) where the Cb atoms of Leu537 of each monomer interact via a hydrophobic packing interaction (Fig 2A,B) Upon replacement of ... head domain of up to 4.3 A, and of exposed parts of the Rossmann domain of up to 2.7 A, are observed For the rest of the homotetrameric assembly, only smaller backbone displacements of up to ... Data processing and evaluation were performed using the origin 7.5 software Crystallization, data collection and renement of the E542K and L537G mutants Crystals of the E542K, L537G and E542K L537W...

Ngày tải lên: 07/03/2014, 03:20

... understanding of the thermal sciences The handbook includes information on thermodynamics and the properties of fluids; the three modes of heat transfer - conduction, convection, and radiation; and ... distinction between the forms of energy that are properties of a system and the forms of energy that are transferred to and from a system is important to the understanding of energy transfer systems HT-01 ... understand the impact that their actions may have on the safe and reliable operation of facility components and systems The Thermodynamics, Heat Transfer, and Fluid Flow handbook consists of three...

Ngày tải lên: 17/03/2014, 15:00

... understanding of the thermal sciences The handbook includes information on thermodynamics and the properties of fluids; the three modes of heat transfer - conduction, convection, and radiation; and ... is being transferred Convection involves the transfer of heat by the mixing and motion of macroscopic portions of a fluid Radiation, or radiant heat transfer, involves the transfer of heat by ... temperature of the inner surface of the pipe is 12 2oF and the temperature of the outer surface is 11 8oF The thermal conductivity of the stainless steel is 108 Btu/hr-ft -oF Calculate the heat transfer...

Ngày tải lên: 17/03/2014, 15:00

... understanding of the thermal sciences The handbook includes information on thermodynamics and the properties of fluids; the three modes of heat transfer - conduction, convection, and radiation; and ... understand the impact that their actions may have on the safe and reliable operation of facility components and systems The Thermodynamics, Heat Transfer, and Fluid Flow handbook consists of three ... understand the characteristics of laminar and turbulent flow EO 1.9 DESCRIBE the characteristics and flow velocity profiles of laminar flow and turbulent flow EO 1.10 DEFINE the property of viscosity...

Ngày tải lên: 17/03/2014, 15:00

... phase of ␣-SnWO4 cannot be obtained and the product obtained is a mixture of SnW3 O9 and SnO2 With pH increasing to basic condition, a mixture of SnO2 and SnO can be obtained The molar ratio of ... the form of hydroxyl groups and oxygen in the ambient environment The adsorbed O2− and HO− groups can trap the electrons from the conduction band of ␣-SnWO4 and induce the formation of a depletion ... Academy of Science in 1996, and PhD degree in chemistry from National University of Singapore in 2000 She is currently a professor in Department of Chemistry and Chemical Engineering of Fuzhou...

Ngày tải lên: 20/03/2014, 13:05

... units of ¯uorescence intensity (A) Cotransfection of CFP/8Tm2 and YFP/8Tm2 (B) Cotransfection of 8NTm1, CFP/8Tm2 and YFP/8Tm2 (C) Cotransfection of CFP/AC8 and YFP/AC8 Ó FEBS 2002 Dimerization of ... nonspeci®c antibody band The arrow heads show the positions of GFP/8Tm2C2 (c 80 kDa) and GFP/8Tm2 (c 55 kDa) The upper bands are probably oligomeric forms The amount of AC8 expressed in all of the transfections ... GFP/8C1 and 8NTm1 were as described in [20] CFP/AC8 and YFP/AC8 were obtained by switching the GFP of GFP/AC8 into CFP and YFP between the restriction enzyme sites Nhe1 and BglII, from pECFP and...

Ngày tải lên: 23/03/2014, 21:21

... Institutes of Health, Office of Research on Women's Health; 1999 U.S Department of Health and Human Services Healthy People 2010 2nd ed With Understanding and Improving Health and Objectives for Improving ... incidence of oral diseases, as well as enhancing the remineralization of tooth enamel and inhibiting the activity of bacterial plaque.2 Such evidence has led to the use of fluoride in a variety of oral ... prevention strategies often focus on efforts to inhibit the development and accumulation of bacterial plaque in the dental and periodontal regions through both mechanical procedures and chemotherapeutic...

Ngày tải lên: 28/03/2014, 13:20

... schools now offer credits and degrees And, with the convenience of earning legitimate credits via the Internet, the amount of credit transfer has exploded Unfortunately for students seeking to transfer ... factors are: • College and/ or state policies on transfer and types of credit: Most colleges and some states have policies concerning the number and type of credits they will transfer from another ... system 3.4 - Understand the transfer function of the performance of higherorder elements 3.5 - Be knowledgeable of different types of transducers 3.6 - Understand and be able to configure circuits...

Ngày tải lên: 29/03/2014, 03:21

... risk of infectious diseases for people with diabetes Diabetes Care 26, 510–513 17 Dofferhoff AS, Nijland JH, de Vries-Hospers HG, Mulder PO, Weits J & Bom VJ (1991) Effects of different types and ... constant but slow rate in the body, and accumulate markedly by aging and diabetes as a result of the increased availability of glucose [4,5] A large body of evidence suggests that AGEs are important ... development of many diseases that involve elevation of AGEs For example, AGEs are found in the retinal vessels of diabetic subjects, and their levels correlate with the severity of the subject’s...

Ngày tải lên: 30/03/2014, 01:20

... with 0.2 lM of p53 peptides or 100 ng of recombinant p53 at 30 °C for 15 in the presence of 4.2 nM of [32P]ATP[cP] (3000 CiÆmmol)1), 10 ng of DNA and 10 units of DNA-PK in 20 lL of reaction buffer ... phosphorylation of recombinant p53 with the phosphorylation of S15A and S37A substituted p53 (p53S15A, p53S37A) in the presence of ssM13 and linearized double-stranded plasmid DNA (Fig 1) In the presence of ... the contrary, preincubation of the DNA-PK for 10 in the presence of 50 lM ATP and ssM13 prior to addition of p53 and [32P]ATP[cP], led to phosphorylation of both p53wt and p53S15A/S37A by 80% (Fig...

Ngày tải lên: 30/03/2014, 15:20

... company's logic of earning money It is a description of the value a company offers to one or several segments of 11 customers and the architecture of the firm and its network of partners for ... Conceptualization of model helps in improving the power of imagination, which can open a new horizon of innovations • Communication and share: Good formulation of a business model is of no use if ... model is depiction of company’s logic of earning money by offering and selling goods and services to make profits (Osterwalder and Pigneur, 2009) 2.2 PROGRESSION OF THE BUSINESS MODEL CONCEPT As outlined...

Ngày tải lên: 01/06/2014, 13:59

... learning modules offered from Hanoi Ag and CTU Long term, increased output of research on postharvest handling of fresh fruit and vegetables grown in Vietnam and subsequent transfer of results in ... subsequent transfer of results in grower publications and via the rural extension network Development of postharvest curriculum for undergrad teaching and teaching of colleagues At the completion of ... by improving the quality and safety of its products Research and training in postharvest physiology is an essential component of these developments, particularly in the areas of food safety and...

Ngày tải lên: 22/06/2014, 13:20

... for each of the following: extent of synovial hyperplasia, mononuclear infiltration, cartilage destruction and pannus formation Antibodies and reagents The following antibodies were grown and purified ... streptavidin-allophycocyanine and streptavidin-PE, and matched antibody pairs for ELISPOT analysis of IFN-γ (R4-6A2 and biotinylated XMG1.2) and IL4 (BVD4 1D11 and biotinylated BVD6-24G2) production ... intravenously, and 24 hours later mice were killed and the distribution of radioactivity in various organs and the rest of the body was measured in a γ-counter (Wallac Counter, Turku, Finnland) Alternatively,...

Ngày tải lên: 09/08/2014, 06:22