immunotherapy of allergic diseases with cpg dna
Báo cáo y học: " Prospects for control of emerging infectious diseases with plasmid DNA vaccines" doc
... mechanism of the induction of immunity after pDNA vaccination is complex [9] and multi-factorial (Figure 1) Figure Proposed mechanism of DNA vaccines Proposed mechanism of DNA vaccines Page of (page ... Manufacturing DNA Vaccines Production of DNA vaccines starts with E coli cells which are transformed with the plasmid of interest These cells are grown and stored frozen in a stock of vials called ... Another approach for the delivery of DNA vaccines is the use of needle-free devices Needle-free injection of DNA Page of (page number not for citation purposes) Journal of Immune Based Therapies and...
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Báo cáo y học: " Primary Prevention of Allergic Diseases: Current Concepts and Mechanisms" pps
... Transcription factors of Th2 cytokines Mechanism Inhibition of synthesis of transcription factors on the level of transcription Inhibition of synthesis of transcription factors on the level of translation ... Activation of TLR-2 Mycobacterial antigens Activation of TLR-9 Activation of TLR-4 ? CpG motifs Lipopolysaccharides Probiotics Induction of Foxp3, TGF-b and IL-10 SIT Parasites Transcription factors of ... effect of orally given apathogenic Escherichia coli strains on the development of atopic dermatitis within the first months of life Probiotics Colonization of the gut by commensal microbes within...
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Báo cáo thú y: ": Cases with manifestation of chemodectoma diagnosed in dogs in Department of Internal Diseases with Horses, Dogs and Cats Clinic, Veterinary Medicine Faculty, University of Environmental and Life Sciences, Wroclaw, Poland" docx
... the results of the standard histopathological examination with hematoxylin-eosin staining, immunohistochemistry was applied with use of a panel of antibodies, specific for tumour cells of neuronal ... dogs, accounting for 5.4% of the examined population and for 12.7% of the examined dogs with cardiac disease A single dog with the diagnosed tumour of the cardiac base of chemodectoma has represented ... et al., Cases with manifestation of chemodectoma diagnosed in dogs in Department of Internal Diseases with Horses, Dogs and Cats Clinic, Veterinary Medicine Faculty, University of Environmental...
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A double blind randomized placebo controlled clinical trial on the supplementation of probiotics in the first six months of life in asian infants at risk of allergic diseases effe
... allergic diseases 1.1.1 Definitions 1.1.2 Epidemiology of Allergic Diseases in Childhood 1.1.3 Immunological basis of atopy and allergic diseases 1.1.4 The microflora ... suggested that disruption of gut microbiota could drive the development of allergic airway response without any previous systemic priming The ‘microflora hypothesis of allergic diseases has been postulated ... development of sensitization and allergic diseases during early infancy The primary aim of this study is therefore to assess the effect of administration of probiotics from birth on the prevention of allergic...
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Báo cáo y học: " Local therapy with CpG motifs in a murine model of allergic airway inflammation in IFN-β knock-out mice" doc
... mice Preliminary data with different dose of CpG administered intranasally with OVA (5 µg, 10 µg or 20 µg) to both strain of mice resulted in similar reduction of percentage of infiltrating eosinophils ... (Table 1) Treatment with CpG- ODN inhibits total number of infiltrating cells in airways in WT but not in IFN-β-/- mice The treatment with µg of CpG administered intranasally with OVA resulted in ... number of cells in OVA-sensitized B10.RIII mice but not in IFN-β-/- Figure recruitment (A), eosinophils (B) Effects of treatment with CpG- ODN on total BALF cell Effects of treatment with CpG- ODN...
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Association studies of genetic polymorphisms found in interleukins 12, 13 and CD14 gene with asthma and allergic diseases
... dried DNA pellet was resuspended with DNase/RNase free water The pellet was then incubated overnight at 37oC To determine the concentration of DNA obtained, 5μl of DNA was mixed with 45μl of distilled ... A260 reading of 1.0 will yield approximately 50 µg of dsDNA or approximately 37 µg of ssDNA The purity of the DNA obtained is measured by the formula: A260 / A280 = 1.8 for pure DNA The DNA was then ... few rounds of the PCR would yield slight changes into the newly synthesized strands of DNA However with following cycles, the DNA that is primed with the primers would yield DNA strands with the...
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Báo cáo y học: "Positioning Effects of KillerRed inside of Cells correlate with DNA Strand Breaks after Activation with Visible Light"
... study The detection of DNA fragments was conducted by two different methods of proof: Figure demonstrates the total DNA as well as the defined DNA strand breaks in nuclei of DU 145 human prostate ... (Typhoon) Defined DNA bands of 200, 400 and (600) bps as prominent degradation products (right picture of the Figure 6) can be detected clearly The length of 200 bp matches with the DNA size of one nucleosome ... the increase of fluorescence intensity inside of the nuclei of DU145 cells Figure visualizes the degree of the DNA damage in nuclei as a result of DNA strand breaks caused by ROS produced by the...
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Báo cáo khoa học: Interaction of selenium compounds with zinc finger proteins involved in DNA repair pdf
... loss of DNA binding, zinc release and/or formation of disulfide bridges [20] With respect to selenium compounds, it has recently been shown that selenite impairs the DNA- binding activity of TFIIIH, ... PM2, I ¼ the percentage of supercoiled PM2 DNA, and II ¼ the percentage of open circular PM2 DNA The overall number of strand breaks per 10 000 bp represents the sum of single-strand breaks and ... the event of diminished XPA DNA binding, a new band of even slower migration appeared (band 3), indicative of high molecular mass DNA protein complexes Similarly, complete inhibition of oligonucleotide...
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Báo cáo hóa học: "Multicriteria Gene Screening for Analysis of Differential Expression with DNA Microarrays" potx
... fall into the category of a two-way layout [21], where each cell in the layout corresponds to a set of replicates of samples from one of the two populations (row) and one of T-time points (column) ... and biological level of significance The statistical level of significance of the test is specified by the false positive rate In contrast, the biological level of significance of the test is specified ... dependent sets of p PCER CIs, is guaranteed to cover at least (1 − α)P of the unknown parameters [26, 30] When the number of P of parameters is random, as occurs when the number of parameters...
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Báo cáo y học: "Diagnostic evaluation of food-related allergic diseases" docx
... is a cornerstone in the evaluation of food allergy It offers an in-office, rapid, and sensitive assessment of allergen sensitization General considerations of skin testing should be discussed ... measures of IgE antibody Specific IgE values have also been used to determine the appropriateness of a food challenge For instance, a specific IgE level of kUa/L in a group of children with a high ... shortness of breath) and/or the cardiovascular system (light-headedness, syncope, hypotension) These reactions can lead to death [4,5] These symptoms typically begin within an hour of ingestion of the...
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Báo cáo y học: "The role of Probiotics in allergic diseases" pptx
... role of probiotics in atopic dermatitis has been extensively studied Prevention of atopic dermatitis The prevention of allergic diseases relies heavily on preventing sensitization to an offending ... composition of gut microbiota of allergic infants a target of bifidobacterial therapy at weaning? Gut 2002, 51(1):51-5 Kirjavainen PV, et al.: Characterizing the composition of intestinal microflora ... mothers with a strong family history of eczema, allergic rhinitis or asthma, and to their infants for the first six months after delivery The frequency of developing atopic dermatitis in the offspring...
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Báo cáo y học: "A comparative study of anxiety and depression in patients with bronchial asthma, chronic obstructive pulmonary disease and tuberculosis in a general hospital of chest diseases" ppsx
... verified by studies performed with patients with respiratory failure, with depression being observed in 30% of patients with moderate failure and in 50% in patients with severe obstructive pulmonary ... 0.05); 49.2% of the sample had moderate to severe depression, and 44.0% of men had depression symptoms compared to 55.6% of women, whilst 21.8% of men had anxiety symptoms compared to 33.3% of women ... are the group with the higher and more severe depression comorbidity Depression in patients with chronic respiratory diseases coexists with anxiety and is related to the chronicity of the disease...
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Báo cáo y học: "Comparing the prevalence of rheumatic diseases in China with the rest of the world" pptx
... persons of Han ancestry might be of interest In summary, Zeng and colleagues have comprehensively summarized the prevalence of different rheumatic diseases in China Their results present a mosaic of ... Prevalence of rheumatic diseases in Taiwan: a population study of urban, suburban, rural differences J Rheumatol 1994, 21:302-306 Silman A, Hochberg MC: Epidemiology of the Rheumatic Diseases 2nd ... prevalence of fibromyalgia in China In addition, given the import of genetic variations in explaining racial and ethnic differences in chronic pain [8,9], an assessment of the prevalence of these...
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Báo cáo y học: "Analysis of bacterial DNA in synovial tissue of Tunisian patients with reactive and undifferentiated arthritis by broad-range PCR, cloning and sequencing" pps
... that this variety of intraarticular bacterial DNA plays in the pathogenesis of ReA and other forms of arthritides However, detection of bacterial DNAs in the ST of patients with arthritis does ... [25,26] All of the cases of ReA were acquired sexually, with arthritis occurring within weeks of an urogenital infection (Table 1) UA was defined as a monoarthritis or oligoarthritis occurring without ... group, bacterial 16S rDNA was amplified in ST samples from three of the seven patients with RA (43%) and from three of the six with OA (50%) Accordingly, the proportion of ST samples from ReA...
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Báo cáo y học: " Diagnosis of systemic toxoplasmosis with HIV infection using DNA extracted from paraffinembedded tissue for polymerase chain reaction: a case report" pptx
... Re-emerging Infectious Diseases (H16-Shinko-6 and H19-Shinko-8), Measures for Intractable Diseases (H20 nannchi ippann 35) from Ministry of Health, Labour and Welfare of Japan, and by Grant of the Strategic ... B1 gene of T gondii was carried out following the previous description of Tachikawa et al [6] The primers used in the assay are summarized in Page of Figure Hematoxylin and eosin stain of bone ... Okubo et al Journal of Medical Case Reports 2010, 4:265 http://www.jmedicalcasereports.com/content/4/1/265 Page of Figure Result of nested PCR for T gondii The detection threshold of nested PCR for...
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Báo cáo y học: "Diagnosis of systemic toxoplasmosis with HIV infection using DNA extracted from paraffinembedded tissue for polymerase chain reaction: a case report" ppt
... Re-emerging Infectious Diseases (H16-Shinko-6 and H19-Shinko-8), Measures for Intractable Diseases (H20 nannchi ippann 35) from Ministry of Health, Labour and Welfare of Japan, and by Grant of the Strategic ... B1 gene of T gondii was carried out following the previous description of Tachikawa et al [6] The primers used in the assay are summarized in Page of Figure Hematoxylin and eosin stain of bone ... Okubo et al Journal of Medical Case Reports 2010, 4:265 http://www.jmedicalcasereports.com/content/4/1/265 Page of Figure Result of nested PCR for T gondii The detection threshold of nested PCR for...
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báo cáo khoa học: " Transcription profiles of mitochondrial genes correlate with mitochondrial DNA haplotypes in a natural population of Silene vulgaris" doc
... whether polymorphism in mt DNA is associated with polymorphism in gene transcription profiles We know of no study to date that has documented variation in transcription profiles of plant mt genes in ... profiles of two mt genes correlate with mt DNA haplotypes The cox1 and atp1 genes were chosen based on the previous reports of their high polymorphism in S vulgaris [16,17] We describe mt DNA ... transcription profiles of the cox1 and atp1 genes in offspring of maternal plants collected from one highly diverse population of S vulgaris in Central Europe We also compare transcript patterns of the...
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Báo cáo y học: " Endothelin-1 in exhaled breath condensate of allergic asthma patients with exercise-induced " pps
... observation of the dynamic of changes in ET-1 levels The monitoring of ET-1 levels 24 hours after exercise using noninvasive methods and correlations of obtained results with other markers of airway ... comparison with the group of healthy volunteers This minute difference could be the consequence of the fact, that the study was performed in the group of mild asthmatics with short time-course of the ... exercise, measurement of FENO and a bronchial provocation test with histamine were performed The study protocol was approved by the Ethics of Research Committee of the Medical University of Bialystok,...
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