immunomagnetic isolation of subcellular organelles from cell lysates

... in blood cells, in particular in mononuclear cells and lymphocytes (16,17,18) The presence of C pneumoniae in the blood cells predetermines the possibility of pathogen dissemination from respiratory ... extracted from the resulting pellet using QIAamp DNA Blood Mini Kit (QIAGEN INC., Valencia, Calif.) according to the manual DNA isolation from infected HL cells Cells were harvested from 24 well ... efficiency of C pneumoniae DNA extraction Sufficient recovery of final PCR product has been also seen when protein A from Staph aureus has been used for isolation of C pneumoniae from whole serum...

Ngày tải lên: 26/10/2012, 08:57

... The mean length of ICU stay before isolation of Aspergillus spp was 32.1 ± 21.4 days Previous use of fluconazole was recorded in eight of the 36 patients (22.2%) with isolation of Aspergillus ... was found in 4% of cases, but in none of the patients was a diagnosis of invasive aspergillosis made In one study [19], because of the lack of reliable diagnostic tools, up to 60% of patients with ... presence of clinical features of pneumonia and isolation of Aspergillus spp from respiratory secretions In contrast, antifungal treatment should not be initiated when Aspergillus spp are recovered from...

Ngày tải lên: 12/08/2014, 22:21

... comparison of ecdysteroid receptors A B Fig CLUSTALW amino acid alignment of N-terminal (A ⁄ B) domains of LdEcR and DmEcR (A) Alignment of EcRA from the two species (B) Alignment of DmEcRB1, ... (A ⁄ B) domains of EcR are also divergent in the two insect species [31] (Fig 1), although all of the isoforms from both species share almost complete identity over a stretch of 35–37 amino acids ... only from changes in the level of receptor molecule activity, but also from changes in stability and intracellular localization Possible differences in these parameters among EcR–USP dimers from...

Ngày tải lên: 18/02/2014, 08:20

... different from cells from the nondiabetic control subjects Similarly, the maximal effects of insulin on the state of tyrosinephosphorylation of the insulin receptor or of IRS1, or of phosphorylation of ... adipocytes from 146 the diabetic patients, compared to an EC50 ¼ 0.02 to 0.03 nm in cells from nondiabetic subjects (Fig 7D and Table 1) The maximal rate of glucose uptake in the fat cells from the ... Danielsson et al cell volume (mean ± SE, n ¼ 5), varied (118 to 255 nmolÆmin)1ÆL)1) from individual to individual The maximal rate of glucose uptake in the fat cells from the group of male patients...

Ngày tải lên: 07/03/2014, 16:20

... bands of approximately 30 or 36 kDa, respectively, were also observed in western blots of S2 cells that have stable expression of Flag-tagged DmPCNA2 or Flag-tagged DmPCNA1 (Fig 2C) Analysis of ... structures of DmPCNA2 and DmPCNA1, using the data from human PCNA (Fig 3B) The possible structures of the DmPCNA2 homotrimer resemble that of the DmPCNA1 homotrimer, except for the small region of the ... (second panel from the left), or antiDmPCNA1 serum (middle panel) The 29 kDa protein band of DmPCNA2 and the 35 kDa protein band of DmPCNA1 are indicated by arrows Crude extracts from S2 cells expressing...

Ngày tải lên: 23/03/2014, 10:20

... Antimicrobial components of cod mucus G Bergsson et al Fig Purification of an antimicrobial component from 12 mg protein ⁄ peptide extract, prepared from skin mucus of cod, by use of HPLC The antimicrobial ... Antimicrobial components of cod mucus A B Fig Purification of antimicrobial components from 20 mg protein ⁄ peptide extract, prepared from the skin mucus of cod, by use of HPLC and monitoring the ... 110, 823–831 14 Zasloff M (1987) Magainins, a class of antimicrobial peptides from Xenopus skin: isolation, characterization of two active forms, and partial cDNA sequence of a precursor Proc...

Ngày tải lên: 23/03/2014, 15:21

... USA)] and Cloning of the bovine MUC15 cDNA by PCR with degenerate primers Isolation of total RNA from the mammary gland of a lactating Danish Holstein cow was performed by means of an RNeasy kit ... coding sequence of the bovine protein To investigate the presence of MUC15 expression in epithelial cells of the human mammary gland, we proceeded to isolate the cellular fraction of human milk ... similar sequence was located The milk cell fraction of lactating tissue contains bud-off epithelial cells, enabling performance of an indirect assay for expression of this possible human homologue...

Ngày tải lên: 24/03/2014, 04:21

... three-dimensional structures of certain domains of nitric oxide synthase isoforms are known [53±57], we are not aware of the three-dimensional structure of the calmodulin binding sites of any isoform being reported ... spectrum) Data from Y + fragmentations give sequence information from the N-terminal end of the peptide (see schematic arrows below the spectrum) For a review of fragmentations of MH+ ions of peptides ... 82±101 of caveolin-1 in the form of glutathione S-transferase fusion proteins have been shown to attenuate the activities of all three isoforms of NOS [62] A novel 10-kDa protein inhibitor of nNOS,...

Ngày tải lên: 31/03/2014, 15:20

... expansion of NK cells IL-2 stimulation of PBMCs was shown to elicit absolute expansion of NK cells and CD56+ T cells, e.g NKT cells, γδ T cells and some αβ/CD8+ T cells [30] The combination of IL-2 ... reduction of the expansion of CD4+ T cells At the donor level, a higher proportion of NK cells tend to be associated, in absence or in presence of 3aG1, with a low proportion of CD4 T cells within ... proliferation/expansion of CD4+ T cells, while not affecting the viability of these cells, highlights an unsuspected regulatory property of 3aG1 molecules on human CD4+ T cells Cellular interaction of azabisphosphonate...

Ngày tải lên: 18/06/2014, 15:20

... extract fatty acid from the bacterial cell- wall and identified with microbial identification system (MIDI, Inc., USA) Isolation of bacteria: One hundred strains of LAB were isolated from selective ... broth without bacterial culture Isolation of cholesterol-lowering lactic acid bacteria 393 Table Number of acid- and bile-tolerant lactic acid bacteria isolated from the human intestine for probiotic ... In vitro cholesterol-lowering test: Among isolated 100 strains of LAB, of Streptococcus strains, 11 of Lactobacillus strains and of Bifidobacterium strains were selected as provisional probiotic...

Ngày tải lên: 07/08/2014, 18:20

... hcae rof ylno devresbo yltnetsisnoc erew selpmas lla ni snoitacifilpma evitisop cificepS noitcefni lacoccocotperts rof evitagen si elpmas enO giF ni nwohs sa rof pb 817 ta selpmas 81 dna rof pb ... derots neht dna tnatanrepus eht fo esahp suoeuqa reppu eht no detcelloc saw AND lairetcaB nim rof 000,6 ta degufirtnec dna nim rof sllec lairetcab gniliob yb detcartxe neht saw AND lairetcab ;retaw ... enilav ,esapil rof snoitcaer evitagen dah setalosi llA nispyrtomyhc -α dna esatahpsohp enilakla rof snoitcaer evitisop dah fo setalosi 81 dna esadinimasoculg-β-lyteca -N dna esaretse rof snoitcaer...

Ngày tải lên: 07/08/2014, 18:21

... selection of additional relevant publications for review and analysis Table provides a comprehensive review of the published cases of small bowel metastases from squamous cell carcinoma (SCC) of the ... Histopathology of resected 10x10cm mass Biopsy of 1x1cm mass about 10cms from the stoma Histopathology of Rt.Hemicolectomy years Biopsy of duodenal lesion Jejunal resection histopathology Confirmation of ... The clinical presentation of a ‘malignant' upper-gastrointestinal bleed due to duodenal metastases from SCC of the cervix is unusual Awareness of such infrequent patterns of metastases in cervical...

Ngày tải lên: 09/08/2014, 02:21

... Despite the accumulation of data from the clinical side, there is a scarcity of information from the biology side because of the difficulty in obtaining basic cell biological data from the two currently ... distribution in chordoma cells showed a significantly high ratio in G1phase, very different from the DNA profile patterns of the other two cell lines These showed a greater number of cells in G1-phase ... the Page of original U-CH1 cells (Figure 1B) [18] Original U-CH1 had 75 chromosomes per cell on average, and our UCH1-N cells averaged 75.34 chromosomes per cell The DNA sequencing data of the...

Ngày tải lên: 09/08/2014, 09:21

... Figure cells from a capable of AML but not normal cells Peptides3arepatient withincreasing the cloning efficiency of Peptides are capable of increasing the cloning efficiency of cells from a ... of phage able to bind cells from leukemia patients Phage clones that bind to leukemia cells were isolated by incubating a library of M13 phage bearing mer peptides with BM mononuclear cells from ... presence of GM-CSF Summary of the effects of different peptides on proliferation and differentiation of AML progenitor cells The biologic effects of different peptides on the behavior of AML progenitor...

Ngày tải lên: 10/08/2014, 22:20

... curves of the bands were processed with the aid of specific MediaCybernetics, Gel-Pro Analyzer Version 6.0 software Molecular cloning of the 2.2 kpb dsRNA from B cinerea CCg378 The dsRNAs of B ... of CF11 cellullose (0.2 g of resin) packed in the minicolumn The initial g cell pellet contained approximately 14 mg of total nucleic acids Therefore, roughly 1.5 to 2.0 g wet weight of cells would ... nucleic acids obtained from the g wet weight of fungal cells Under these conditions, the amount of total dsRNA recovered from a minicolumn was approximately μg, since of the 10 μL obtained, only...

Ngày tải lên: 11/08/2014, 21:21

... isolated from mosquitoes collected in these areas [12,13] Molecular epidemiological study showed that all of the JEV isolates obtained either from mosquitoes or from clinical samples of human ... first time from CSF samples of JE patients in China Previously, partial sequences of JEV GI were detected in specimens of meningitis patients in Japan [14] and in JE patients in an outbreak of China ... number of nucleotide substitutions per site Sequences from this study are in boldface Viruses were identified by using the nomenclature of virus strain/ GenBank number/country/year of isolation/ origin...

Ngày tải lên: 12/08/2014, 02:20

... species [15] Most of the surveillance studies [3,4,7,8] have focused on the detection of AIVs from waterfowl and shorebirds while only a few reports are available on isolation of AIV from raptors ... the first report on isolation of AIV in a Bald eagle from the U.S The bald eagle in question was recovered from Gloucester County, Virginia in March, 2008 In general, samples from injured raptors ... Sequences of A/greenwinged teal/Ohio/430/1987(H1N1) (CY011040) and A/ mallard/Ohio/37/1986(H2N1) (CY021125) were homologous to those of the bald eagle isolate Sequences of all eight segments from...

Ngày tải lên: 12/08/2014, 04:20

... the saline of some of the samples to a final concentration of 1% in the agar-gels and the incubation saline About 500 ml of crushed ice was added to some of the jars after the removal of the agar-gels ... Effect of incubation time, glucose and cooling Here, we tested the effect of incubation time of the liver agar-gels (3 or h), and addition of glucose to the saline plus the Table Impact of liver ... Impact of liver blending time, incubation time of agar-gels, and addition of gentamycin on numbers of A suum larvae (mean±SD) recovered from liver tissue of pigs Every hour gels were transferred to...

Ngày tải lên: 12/08/2014, 15:20

... number of colonies of infected cells recovered at each stage of the selection was determined for each of the four pools (Table 1) The number of colonies of wild-type Rat2 cells exposed to the ... Control cDNA pB1-11 pC1-2 Rat2 Cells RC-2 subclone Figure Lack of effect of suppressor cDNAs in wild-type cells Lack of effect of suppressor cDNAs in wild-type cells Rat2 or a virus-sensitive ... recovered from five of the six lines Page of 10 (page number not for citation purposes) Retrovirology 2004, 1:30 http://www.retrovirology.com/content/1/1/30 Schematic of protocol for isolation of suppressor...

Ngày tải lên: 13/08/2014, 13:20

... isolated from the culture vegetal cell suspension This study aims to evaluate the in vivo and in vitro antitumor effects of acetylshikonin extracted from the cell suspension cultures of Arnebia ... (n growth of A549 Inhibitory effects ofgrowth curve assaythe = 3) Inhibitory effects of acetylshikonin on the growth of A549 determined by cell growth curve assay (n = 3) Figureweights of LLC inand ... (USA) Human hepatocellular carcinoma cell line Bel-7402 was obtained from the Cell Bank of the Chinese Academy of Sciences The cells were cultured in RPMI 1640 (Gibco, USA) supplemented with 10%...

Ngày tải lên: 13/08/2014, 15:21