immunogenic myeloma cell death to trigger activation of dcs and to enhance cross presentation of dcs
Báo cáo y học: "A genomic approach to investigate developmental cell death in woody tissues of Populus trees" pot
Ngày tải lên :
14/08/2014, 14:21
... Moreau et al cell walls The final phase in maturation of both vessel elements and fibers is cell death and autolysis of the cell contents Xylem -cell death involves a range of morphological and nuclear ... developmental phases of cell expansion and bulk secondary cell wall deposition, and were approaching cell death (see [13], and corresponding to zone B in Figure 1) Cell death of the fibers is marked ... regulators of cell death Analysis of the most abundant transcripts in the fiber -cell death library yielded a list of candidate genes with high expression levels In order to identify fiber -cell death...
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Báo cáo khóa học: Noncytotoxic ribonuclease, RNase T1, induces tumor cell death via hemagglutinating virus of Japan envelope vector ppt
Ngày tải lên :
23/03/2014, 12:20
... T1, cytotoxic to cancer cells This cytotoxicity is stronger than that of onconase Furthermore, this RNase T1 induces the apoptotic cell death program Because its cytotoxicity is not specific to tumor ... assayed for cytotoxicity on two types of human tumor cell lines: human erythroleukemia cell line K-562 and human tongue carcinoma cell line SAS First, we examined the cytotoxic effect of RNase T1 ... leiomyoblastoma cell line G-402 and Fig Cytotoxic effect of RNase T1/PS/HVJ in human tumor cell lines K-562 cells (circles) or SAS cells (squares) were incubated for 20 h at 37 °C in the presence of different...
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Báo cáo khoa học: PKA independent and cell type specific activation of the expression of caudal homeobox gene Cdx-2 by cyclic AM pptx
Ngày tải lên :
30/03/2014, 16:20
... factors and signaling molecules that regulate the expression of this promoter We found that cotransfection of the Cdx-2 cDNA led to upregulation of the expression of the Cdx-2 gene promoter in cell ... InR1-G9 cell line is defective in PKA activity and cotransfection of PKAc restores its PKA activity Finally we tested the effect of H-89, a known inhibitor of PKA, on the expression of both the ... IBMX failed to stimulate the expression of proglucagon gene mRNA and its promoter in this cell line, in contrast to significant activation by forskolin ⁄ IBMX in primary pancreatic islet cell cultures...
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Tài liệu Báo cáo khoa học: Minor capsid proteins of mouse polyomavirus are inducers of apoptosis when produced individually but are only moderate contributors to cell death during the late phase of viral infection ppt
Ngày tải lên :
16/02/2014, 09:20
... inducers of apoptosis We further examined the character of cell death induced by VP2 or VP3 proteins To assess the contribution of apoptosis to toxicity, we evaluated the cleavage of both effector ... proteins: inducers of cytotoxicity S Huerfano et al A B Fig Influence of caspase inhibition on cell death (A) Measurement of cell toxicity by LDH release into medium after treatment of 3T3 cells expressing ... levels of apoptotic markers during the late stages of MPyV infection However, comparison of the levels of apoptotic markers and cytotoxicity during the replication of wt MPyV, and replication of...
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Báo cáo khoa học: Apoptosis and autophagy: BIM as a mediator of tumour cell death in response to oncogene-targeted therapeutics pptx
Ngày tải lên :
16/03/2014, 00:20
... complexes: mTORC1 (composed of mTOR, mLST8 and raptor) regulates cell growth via the effectors S6K1 and 4E-BP1, whilst BIM as a mediator of tumour cell death mTORC2 (composed of mTOR, mLST8 and rictor) ... inhibition of TOR will increase BIM levels and contribute to tumour cell death However, a recent study suggests that repression of BIM might not be a direct effect of TOR mTOR exists in mammalian cells ... survival of CML cells led to the development of the tyrosine kinase inhibitor imatinib (STI571, Gleevec), which is a potent inhibitor of BCR–ABL, platelet-derived growth factor receptor (PDGFR) and...
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Báo cáo khoa học: Mitochondria regulate platelet metamorphosis induced by opsonized zymosan A – activation and long-term commitment to cell death docx
Ngày tải lên :
16/03/2014, 04:20
... Bax and Bak translocation to the mitochondria and endogenous generation of hydrogen peroxide, which stimulates cytochrome c release and activation of caspases and [22] Thus, OPZ appears to be ... PS and can be induced by activation of the pro-apoptotic Bcl-2 family member Bax [3] Hence, mitochondria integrity and function appear to act as general regulators of platelet fate in terms of ... generation and apoptotic-like events In fact, mitochondrial damage has been reported to be a key step in platelet apoptosis, and mitochondrial membrane potential (MMP) is lost during storage and under...
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Báo cáo khoa học: Mitochondria regulate platelet metamorphosis induced by opsonized zymosan A – activation and long-term commitment to cell death potx
Ngày tải lên :
16/03/2014, 04:20
... chitosanase could function as a resistance factor against the toxic effect of chitosan Besides a strictly metabolic function, consisting of the endohydrolysis of high molecular mass chitosan into ... 5-bromo-4-chloro-3-indolyl-b-d-galactopyr- Active site residues of family 46 chitosanase anoside and isopropyl thio-b-d-galactoside, because the empty pAlter-1 vector directs b-galactosidase synthesis in E coli and false ... proportions of the V148T chitosanase-expressing cells and chitosanase-negative cells and plating them on media with various salt compositions Both colonies could be distinguished thanks to supplementation...
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Báo cáo sinh học: "The bioenergetic signature of isogenic colon cancer cells predicts the cell death response to treatment with 3-bromopyruvate, iodoacetate or 5-fluorouracil" pdf
Ngày tải lên :
18/06/2014, 19:20
... cell- death pathway in response to chemotherapy HCT116 cells were treated as indicated [11] to produce cells with low (G-cells), medium (M-cells) and high (SM-cells) bioenergetic signatures Cells ... (annexin-positive) cells and the upper right and left quadrants corresponds to dead (PI positive) cells Histograms shown are the means ± SEM of the percentage of apoptotic (annexin V positive) (B) and necrotic ... of the relative relevance of cellular energy provision pathways [9,11], and the potential to execute cell death in response to the metabolic inhibitors, 3BrP and IA, and the anti-metabolite 5-FU...
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Báo cáo sinh học: "Inhibition of mitotic kinase Aurora suppresses Akt-1 activation and induces apoptotic cell death in all-trans retinoid acid-resistant acute promyelocytic leukemia cells" ppt
Ngày tải lên :
18/06/2014, 19:20
... proliferation of leukemic cells in AML and inhibition of activation of Akt can result in suppression of cell growth [40,41] In the present study, phosphorylation of Akt-1 and GSK3b, the downstream of Akt-1, ... suppresses the proliferation of NB4-R2 cells and induces cell apoptosis NB4-R2 cells were incubated with increasing doses of VX-680 (1, 2, and 10 nM) for 24 hr and 48 hr (A) Cell viability was measured ... of a number of key cellular regulatory pathways, including cell proliferation pathway, cell survival pathway, caspase activation pathway, tumor suppressor pathway, death receptor pathway, mitochondrial...
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báo cáo hóa học: " Cyclooxygenase-2 mediates microglial activation and secondary dopaminergic cell death in the mouse MPTP model of Parkinson''''s disease" pptx
Ngày tải lên :
19/06/2014, 22:20
... oxidative and inflammatory damage to the neurons, which in turn progresses to the secondary damage /death which is coupled to the release of factors that initiate another cycle of oxidative and inflammatory ... A and B was used, but included only MPTP-treated values and assigned value of for no treatment and 10, 30 and 50 for increasing dosage of valdecoxib The results were similar to those of A and ... correlation of the amount of daily valdecoxib to TH- and Nissl-stained neuronal numbers (both rs ≥ 0.80) and a strong negative correlation of the dosage of valdecoxib to microglial activation...
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báo cáo hóa học:" The bioenergetic signature of isogenic colon cancer cells predicts the cell death response to treatment with 3-bromopyruvate, iodoacetate or 5-fluorouracil" ppt
Ngày tải lên :
20/06/2014, 03:20
... cell- death pathway in response to chemotherapy HCT116 cells were treated as indicated [11] to produce cells with low (G-cells), medium (M-cells) and high (SM-cells) bioenergetic signatures Cells ... (annexin-positive) cells and the upper right and left quadrants corresponds to dead (PI positive) cells Histograms shown are the means ± SEM of the percentage of apoptotic (annexin V positive) (B) and necrotic ... of the relative relevance of cellular energy provision pathways [9,11], and the potential to execute cell death in response to the metabolic inhibitors, 3BrP and IA, and the anti-metabolite 5-FU...
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báo cáo hóa học:" Inhibition of mitotic kinase Aurora suppresses Akt-1 activation and induces apoptotic cell death in all-trans retinoid acid-resistant acute promyelocytic leukemia cells" ppt
Ngày tải lên :
20/06/2014, 03:20
... proliferation of leukemic cells in AML and inhibition of activation of Akt can result in suppression of cell growth [40,41] In the present study, phosphorylation of Akt-1 and GSK3b, the downstream of Akt-1, ... suppresses the proliferation of NB4-R2 cells and induces cell apoptosis NB4-R2 cells were incubated with increasing doses of VX-680 (1, 2, and 10 nM) for 24 hr and 48 hr (A) Cell viability was measured ... of a number of key cellular regulatory pathways, including cell proliferation pathway, cell survival pathway, caspase activation pathway, tumor suppressor pathway, death receptor pathway, mitochondrial...
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báo cáo hóa học:" Microarray analysis of Foxl2 mediated gene regulation in the mouse ovary derived KK1 granulosa cell line: Over-expression of Foxl2 leads to activation of the gonadotropin releasing hormone receptor gene promoter" pptx
Ngày tải lên :
20/06/2014, 07:20
... ratio of 4:1 Fugene to total DNA and added to cells Luciferase assays Transfected cells were incubated for 24 hours at 37°C and washed times with phosphate buffered saline Cells were lysed and ... other hand, the VP16 activation domain mechanism of transactivation is the result of interactions with a variety of factors including histone acetylases, basal transcription factors, and the ... up-regulation of steroidogenic and apoptotic regulatory genes via activation of phosphatidylinositol-dependent kinase/AKT in bovine granulosa cells Reproduction 2010, 139:139-151 39 Goto M, Iwase A, Ando...
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Báo cáo y học: "Higher susceptibility to Fas ligand induced apoptosis and altered α modulation of cell death by tumor necrosis factor-α in periarticular tenocytes from patients with knee joint osteoarthritis" ppt
Ngày tải lên :
09/08/2014, 01:23
... [8,9] Apoptosis is a physiological process and is a highly selective way to eliminate aged and injured cells In addition to internal pathways that trigger apoptosis mainly in response to cytotoxic ... downstream of Fas may alter significantly the susceptibility of cells to Fas induced cell death [17] However, the expression of Fas receptor on the cell surface together with the levels of soluble ... Takeno M, Saito N, Yokoe T, Kaneko A, Asai T, Sakane T: Modulation by proinflammatory cytokines of Fas/Fas ligand-mediated apoptotic cell death of synovial cells in patients with rheumatoid arthritis...
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Báo cáo y học: " The mode of lymphoblastoid cell death in response to gas phase cigarette smoke is dose-dependent" potx
Ngày tải lên :
12/08/2014, 14:20
... induces apoptotic and necrotic cell death in CCRF-CEM cells GPS induces apoptotic and necrotic cell death in CCRF-CEM cells CCRF-CEM cells were exposed to various doses of GPS (1, or puffs) and stained ... manner Cytotoxicity of GPS-exposed cells increases in a dose- and Cytotoxicity of GPS-exposed cells increases in a dose- and time-dependent manner Cytotoxicity was measured in terms of LDH release ... Figure Activation of caspase-3 in response of cell exposure to GPS Activation of caspase-3 in response of cell exposure to GPS Confocal microscopic examination of CCRF-CEM cells exposed to puffs...
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Báo cáo y học: " Legionella pneumophila infection induces programmed cell death, caspase activation, and release of high-mobility group box 1 protein in A549 alveolar epithelial cells: inhibition by methyl prednisolone" pot
Ngày tải lên :
12/08/2014, 15:21
... to identify bacterial trigger factor(s) for cytotoxicity Finally, we examined the influence of methyl prednisolone, as an inhibitor of cell injury, on DNA fragmentation, caspase activation, and ... development and tissue homeostasis, and in modulating the pathogenesis of a variety of diseases [21] A number of pathogens cause host cell death with features of apoptosis [22-24] Pyroptosis is ... Therefore, the mode of cell death could be apoptosis and/ or pyroptosis, induced by either death- receptor signaling or mitochondrial stress In this study, methyl prednisolone had an anti-apoptotic effect...
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Reactive oxygen species mediated regulation of the na+ h+ exchanger, NHE 1 gene expression a new mechanism for tumor cells resistance to apoptotic cell death
Ngày tải lên :
16/09/2015, 08:31
... involvement of mitochodria In cell- surface receptor induced apoptosis, activation of Fas or TNF receptor leads to the activation of initiator caspase 8, followed by the activation of downstream effector ... differences between Apoptotic and Necrotic types of Cell Death Fig B Death- Receptor mediated Apoptosis Fig C Mitochondial pathway of Apoptosis Fig D Wide array of functions attributed to Reactive Oxygen ... Table of contents ii Abstract vi List of figures vii List of abbreviations x Chapter I Introduction I.1 Cell Death I.1.a Types of Cell Death I.1.a Programmed cell death or apoptosis I.1.b Apoptotic...
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Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt
Ngày tải lên :
18/02/2014, 18:20
... leukocytes to inflammatory sites [14,15] and adhesion of polymorphonuclear cells and monocytes to CNS cells [16,17] Schwann cells (SCs) are glia cells found in the PNS In addition to their roles ... the presence of cytokines Natural ligands of ICAM- 1and LFA-1 are expressed on the surface of T and B lymphocytes, natural killer cells, monocytes, macrophages and granulocytes [33], and interaction ... phosphorylated ERK, p38 and JNK (p-ERK, p-p38 and p-JNK) and total ERK, p38 and JNK (tERK, tp38 and tJNK) (B) The ratio of phosphorylated to total ERK (p44 ⁄ 42), p38 and JNK at each time point...
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Tài liệu Báo cáo khoa học: Synergistic activation of signalling to extracellular signal-regulated kinases 1 and 2 by epidermal growth factor and 4b-phorbol 12-myristate 13-acetate pptx
Ngày tải lên :
19/02/2014, 16:20
... phosphorylation of iEGFR causes it to recycle back to the cell surface In addition it causes a decreased EGF binding affinity and tyrosine kinase activity of the receptor Also shown are the stimulators and ... (1999) The repertoire of fos and jun proteins expressed during the G1 phase of the cell cycle is determined by the duration of mitogen-activated protein kinase activation Mol Cell Biol 19, 330–341 ... growth factor (EGF) receptor density controls mitogenic activation of normal rat kidney (NRK) cells by EGF J Cell Physiol 174, 9–17 50 Morrison, P., Saltiel, A.R & Rosner, M.R (1996) Role of mitogen-activated...
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Tài liệu Báo cáo Y học: Phosphorylation of initiation factor-2a is required for activation of internal translation initiation during cell differentiation ppt
Ngày tải lên :
22/02/2014, 07:20
... translational inhibitors often belong to mRNAs encoding proteins involved in cell growth and differentiation such as growth factors, receptors, transcription factors, protooncogenes, and cytokines [10] ... arrest and polyploidy [27], DNA content evaluation by flow cytometry was chosen as a tool to detect the reduced number of cells in S-phase and enhanced number of cells harbouring two- to fourfold ... interferons and cell death: guardians of the cell or accomplices of apoptosis Semin Cancer Biol 10, 103– 111 37 Jagus, R., Joshi, B & Barber, G.N (1999) PKR, apoptosis and cancer Int J Biochem Cell...
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