immunocytochemical detection of proteins in intact and permeable pc12 cells
Detection of cracks in plates and pipes using piezoelectric materials and advanced signal processing technique
Ngày tải lên :
16/09/2015, 15:53
... over-decomposition of signal during resulting in spurious harmonics will be proposed To develop a methodology for locating and determining the geometry of linear and nonlinear cracks in thin homogeneous ... reduction in E at D13 using (a) points and (b) 16 points 45 2.13 Relative change in mode for 50% reduction in E at D14 using (a) points and (b) 16 points 45 ix 2.14 Relative change in mode for 20% increase ... increase in E at D10 using (a) points and (b) 16 points 46 2.15 Relative change in mode for 50% reduction in E at D08 and D10 using (a) points and (b) 16 points 47 2.16 Relative change in mode...
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Báo cáo khoa học: Protein transport in organelles: Dual targeting of proteins to mitochondria and chloroplasts ppt
Ngày tải lên :
07/03/2014, 03:20
... provided, and the insights that may be gained from a greater understanding of the mechanism of dual targeting for the targeting of location-specific proteins is discussed Targeting signals and mature proteins ... [17,18] Instead, we provide an overview the signals, proteins, receptors, sorting of dual-targeted proteins and why dual targeting may occur Finally, an outline of the future challenges in this ... mitochondrial and chloroplastic targeting signals in terms of arginine and serine content (i.e not as high as in mitochondrial targeting signals) and may be slightly enriched in hydrophobic residues In...
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Báo cáo khoa học: Esculentin-1b(1–18) – a membrane-active antimicrobial peptide that synergizes with antibiotics and modifies the expression level of a limited number of proteins in Escherichia coli doc
Ngày tải lên :
16/03/2014, 00:20
... peptide, and dermaseptin, from frog skin [11]; (c) drosocin and pyrrhocoricin, from insects [12]; and (d) Bac-7(1–35), corresponding to the 35-residue N-terminal region of Bac-7 from bovine neutrophils ... fingerprinting, reported in Table In particular, reductions in the expression levels of a number of OM proteins (OMPs), such as OMPc, nmpC, and OMP F, all of which form passive diffusion pores allowing ... of E coli, · 106 cells were mixed with lm SYTOX Green in NaCl ⁄ Pi for in the dark After addition of peptide, the increase in fluorescence, owing to the binding of the dye to intracellular DNA,...
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in situ detection of dna damage, methods and protocols
Ngày tải lên :
11/04/2014, 09:47
... colorimetric and fluorescent staining of cultured cells and tissues is presented thereby allowing investigators to utilize the TUNEL staining procedure with a minimal investment in laboratory reagents and ... in PBS Spin down cells and aspirate buffer Resuspend in 0.5–1 mL of PBS Examine the cells on a flow cytometer using an excitation wavelength of 488 nm, and detecting the green TUNEL signal, indicative ... Incubate while rocking for h at room temperature in the dark in 100 µL of staining buffer containing 10 µg/mL of FITC-conjugated streptavidin Wash by diluting the sample with mL of 2× TB buffer...
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báo cáo hóa học:" Assessing the clinical utility of measuring Insulin-like Growth Factor Binding Proteins in tissues and sera of melanoma patients" potx
Ngày tải lên :
18/06/2014, 15:20
... role of the insulin-like growth factor binding proteins and the IGFBP proteases in modulating IGF action Endocrinol Metab Clin North Am 1996, 25(3):591-614 Rajaram S, Baylink DJ, Mohan S: Insulin-like ... acquisition of data, analysis and interpretation of data, and the writing of this manuscript MAW participated in the analysis and interpretation of data and the writing of this manuscript PJC performed ... design and coordination, supervised the analysis and interpretation of the data, and writing the 10 11 12 13 Tao Y, Pinzi V, Bourhis J, Deutsch E: Mechanisms of disease: signaling of the insulin-like...
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Báo cáo hóa học: " Sensitive and molecular size-selective detection of proteins using a chip-based and heteroliganded gold nanoisland by localized surface plasmon resonance spectroscopy" pptx
Ngày tải lên :
21/06/2014, 04:20
... sensitive and molecular size-selective detection of a protein The enlarged schematic diagram shows the surface morphology of a heteroliganded gold nanoisland and the binding principle of a protein in ... annealing procedure of the surface, dimensions of the gold nanoislands were in the range of 40-80 nm in diameter [26,27] The binary SAM containing MPA and DT was fabricated on the gold nanoislands ... permit the sensitive and size-selective detection of a protein over an aggregated species in a variety of formulations (Figure 1A) The binding interaction between the proteins and the nanoholes...
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Báo cáo sinh học: "Adaptive evolution of centromere proteins in plants and animals" ppt
Ngày tải lên :
06/08/2014, 18:21
... DNAbinding proteins would maintain an interface with the conserved kinetochore machinery Indeed, regions of CENP-C that show evidence of positive selection include DNAbinding and specificity regions, in ... Comparisons of CENP-C proteins in animals, yeast and plants The CENPC motif and conserved regions found at the termini of CENP-C proteins are indicated For pairwise comparisons of protein-coding sequences, ... evolution of DNA-binding specificity in these regions Targeting of native CENP-C proteins depends on other centromere proteins that vary according to species [45], but the dependence of CENP-Cs...
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Báo cáo khoa học: "Detection of betanodaviruses in apparently healthy aquarium fishes and invertebrates" docx
Ngày tải lên :
07/08/2014, 18:21
... aciremA fo setatS detinU eht morf fo dna aisenodnI morf 31 fo ,eropagniS morf 85 fo ,napaJ morf selpmas 85 eht fo :swollof sa erew RCP detsen yb surivadon rof evitisop sngis lacinilc on htiw hsif ... 06 rof Co27 ta pets noisnetxe lanif a dna ,s 02 rof Co75 ta gnilaenna ,s 03 rof Co49 ta noitarutaned fo selcyc 03 htiw )napaJ ,arakaT( esaremylop qaT xE gnisu detcudnoc saw RCP ,nim 06 rof Co54 ... sitaboilyM( yar elgae nommoC )acinopaj sirtneconoM( hsif enoceniP )sutagirts suhtnacorciM( rellab toof yepirtS )arimsak sunajtuL( reppans depirts eulB )inisanrof airotcaL( hsifwoc kcabnrohT )sutxim...
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Báo cáo y học: "Post-translational aging of proteins in osteoarthritic cartilage and synovial fluid as measured by isomerized aspartate" pot
Ngày tải lên :
09/08/2014, 14:20
... Identification of several populations of large and small proteoglycans and of hyaluronic acid-binding proteins in successive cartilage extracts Biochem J 1991, 273:579-585 Collins D: The Pathology of Articular ... assumptions of normality, linearity, and homoscedasticity essential to methods of linear regression modeling, we logarithmically transformed IsoAsp, GAG, and protein values and used the D'Agostino and ... protective role in preventing degradation of collagen fibrils [28] With increasing loss of proteoglycan, there is increasing degradation of collagen and other matrix components [29], and this is...
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báo cáo khoa học: "Identification and analysis of phosphorylation status of proteins in dormant terminal buds of poplar" pptx
Ngày tải lên :
11/08/2014, 11:21
... residue in the Lhcb1 isoforms of Arabidopsis and spinach (Figure 2b) Table Conservation of phosphosites and phosphoproteins between poplar and Arabidopsis Phosphoproteins Number 1) Proteins unique ... 4b) In this study, most of the identified phosphoproteins were involved in binding and catalytic activity, consistent with previous studies [22,32,33] Page of 16 Potential protein kinases involved ... genes and proteins involved in protein biosynthesis play a role in the mechanism of bud dormancy release [12,60,101] Phosphorylation of ribosomal proteins can affect protein synthesis by altering...
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Báo cáo y học: " Detection and quantitation of HPV in genital and oral tissues and fluids by real time PCR" pot
Ngày tải lên :
12/08/2014, 04:20
... Linear plots of standards were similar in multiplex reactions compared to plots obtained when using single primer pairs and probes indicating that type-specific amplification of individual standard ... specific amplification and detection of amplicons This assay had a linear range of detection over orders of magnitude, allowing identification/typing of viral genomes and detection of viral load [11] ... here may provide insight into the epidemiology and natural history of HPV infections within individuals and groups, providing prognostic capability and leading to the development of intervention...
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Báo cáo khoa học: " Comparisons of Sampling Procedures and Time of Sampling for the Detection of Salmonella in Danish Infected Chicken Flocks Raised in Floor Systems" pptx
Ngày tải lên :
12/08/2014, 15:20
... numbers of Salmonella positive flocks in routine samples and in 5sock-samples for the remaining 26 broiler flocks In 16 flocks, where Salmonella was detected in the routine samples (ranging from ... where Sal- Comparing routine sock samples and 5-socksamples in broiler flocks More than one Salmonella serovar was found in Table Comparison of results of sampling of 26 Salmonella infected broiler ... number of flocks Acta vet scand vol 43 no 1, 2002 1 10 26 Procedures for detection of Salmonella in chicken Table Statistics used for, and results of comparisons between sampling of 26 Salmonella infected...
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Báo cáo sinh học: "A microsatellite-based analysis for the detection of selection on BTA1 and BTA20 in northern Eurasian cattle (Bos taurus) populations" doc
Ngày tải lên :
14/08/2014, 13:21
... genomic interval of significant LD is comparable with the findings of hitchhiking around two anti-malarial resistance genes in humans [58] and microsatellite hitchhiking mapping in the three-spined ... Finncattle and Northern Finncattle) It has the advantage of being able to take into account a wide range of potential parameters simultaneously and giving results that are robust regarding the starting ... DIK5019 and AGLA17) as outliers showing footprints of selection in the analyses, including all 10 populations, at the 5% significance level Of the four significant loci, three (SOD1, BMS2461 and...
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Roles of tuberous sclerosis complex proteins in autophagy and cell death
Ngày tải lên :
10/09/2015, 09:30
... swelling and the final rupture of the cells will causes the release of intracellular structures to the surroundings In contrast to apoptotic cells, the nuclei of necrotic cells remain intact (Vandenabeele ... UVRAG-Bif1-Beclin 1-p150-PI3KC3 (Janku et al., 2011) In contrast, the RUN domain and cysteine-rich domain containing Beclin interacting protein (Rubicon) interacts with UVRAG–Beclin 1–hVps34–p150 ... fetal bovine serum free fatty acids FAK-family-interacting protein of 200kD FADD-like IL-1β-converting enzyme FLICE-inhibitory protein FKBP12-rapamycin binding GTPase Activating Protein glyceraldehyde-3-phosphate...
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Molecular interactions of tectonin proteins in host and pathogen recognition
Ngày tải lên :
14/09/2015, 08:38
... docking of the GBP-CRP interaction 93 3.40 Binding affinity of infected GBP 94 3.41 Effect of infection upon GBP-CRP interaction with LA 95 3.42 Effect of calcium on infected proteins binding ... the Tectonin-domain containing proteins in the vertebrates, and especially so in the human We found widespread occurrence of Tectonin domain-contaning proteins in the human, and amongst many ... energy minimization 2.1.4 Protein-protein and protein-ligand docking Preliminary flexible ligand and rigid receptor docking was performed using MOE Site Finder and Docking modules The top 30 docking...
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Isolation and detection of bacteria in soil, ash and sediment with the contamination of paraquat and glyphosate
Ngày tải lên :
28/04/2016, 13:40
... http://en.wikipedia.org/wiki/Polymerase_chain_reaction) 2.3.3 Application of PCR At the present, PCR is very valuable in a number of newly emerging laboratories and clinical techniques, include DNA cloning for sequencing, detection of ... 0.020–0.039 in Coarse sand to 0.25–0.5 mm 0.010–0.020 in Medium sand to 125–250 µm 0.0049–0.010 in Fine sand to 62.5–125 µm 0.0025–0.0049 in Very fine sand to 3.9–62.5 µm 0.00015–0.0025 in Silt Mud ... cycle of PCR Figure 3.1 PCR process for 16S rDNA 24 In this process, µL of DNA Ladder was added and mixed 20 µl of products of PCR and µl of 6x Loading Dye were put into gel which contained 0.5...
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Cambridge.University.Press.Allegories.of.Union.in.Irish.and.English.Writing.1790-1870.Politics.History.and.the.Family.from.Edgeworth.to.Arnold.Oct.2000.pdf
Ngày tải lên :
21/09/2012, 10:39
... lyric, ‘‘Act of Union’’ (), the coupling of England and Ireland issues in the conception of ‘‘an obstinate fifth column,’’ ‘‘the heaving province’’ of Ulster.¹ Identifying the masculine position ... Allegories of Union in Irish and English writing English discursive violence, another legacy of the rhetoric of empire as it has been institutionalized in ways of speaking and writing, learning and ... the landed protestant minority in Ireland and the British government in the s, especially in view of the threat from France And each dominant force moved in its own way – and in its own interests...
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Báo cáo y học: "Functional genomics analysis of low concentration of ethanol in human hepatocellular carcinoma (HepG2) cells. Role of genes involved in transcriptional and translational processes"
Ngày tải lên :
31/10/2012, 15:28
... and ethanol-induced mucosal injury in the upper gastrointestinal tract leading to increase in the permeability of the gut mucosa to endotoxins [43, 44] These factors are involved in ethanol-induced ... ingestion of ethanol induces a completely different pattern due to the reported ethanol-induced expression and activation of cytokines and chemokines in monocytes and macrophages (including Kupffer cells) ... G-Protein-MAPK, NGF, and PDGF signaling This finding corroborates the interaction observed between various intracellular signaling pathways and apoptosis induced by 1mM concentration of ethanol in...
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An Overview into the Semantic of Questions in English and Vietnamese
Ngày tải lên :
22/01/2013, 09:28
... comparing and contrasting linguistics became popular thanks to the emergence of new lands, new communities, and new languages Sine 1970s, comparing and contrasting linguistics has been playing ... request for information The semantics refers to the close and intense relationship between the form and the meaning of a sentence (Thiêm, 2004) Comparing and contrasting questions in terms of their ... and English learners have encountered a lot of difficulties practicing questions in speaking and learning Therefore, this paper aims to compare and contrast the differences and similarities of...
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focuses on expressions of criticizing in American and Vietnamese e-newspapers in the light of Directness/Indirectness
Ngày tải lên :
06/04/2013, 08:43
... sheet of paper printed and distributed, at stated intervals for conveying intelligence of passing events, advocating opinions, etc.; a public print that circulates news, advertisements, proceeding ... methods of indirect criticism when expressing their point of view This, on one hand, reduces negative reactions; and, on the other hand, the information becomes more vivid and interesting to readers ... normal and denotative meanings It brings the shade of meaning of being humorous and witty or ironical and satirical 28 Obama's “team of rivals” share big-picture views (The Washington Post) “5 sinh...
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