immunochemical physicochemical conformational and structural analysis to identify sfy immunodominant epitopes

... inhibitor whereas maltose and cyclohexaamylose behaved as competitive inhibitors of the Calystegia b-amylase (Fig 2B) Glucose was only a weak inhibitor (Ki ¼ 262 mM ) when compared to maltose ... surfaces was 1.4 A and a standard ˚ Stern layer was used to exclude ions from the 2.0 A molecular surface [37] The inner and outer dielectric constants applied to the protein and the solvent were, ... maltose and cyclohexaamylose was determined from Lineweaver –Burk plots, and inhibitor constants were determined from Dixon plots RNA isolation, construction and screening of cDNA library Total...

Ngày tải lên: 22/02/2014, 07:20

... among pollen and food allergic patients [18] Finally and recently, a 46 kDa allergen has been reported and its internal peptide sequences shown to have some sequence similarity to cytochrome c ... Fullerton, CA, USA) using a linear gradient of 5–60% acetonitrile in 0.06% trifluoroacetic acid and a flow rate of mLÆmin)1 Peptide elution was monitored at 220 nm and all fractions were collected and ... two chromatographic steps using a CM-TSK column and reverse phase HPLC When fraction AS1 in starting buffer was applied to the CM-TSK column, one major peak (C1) and two minor peaks (C2 and C3)...

Ngày tải lên: 07/03/2014, 12:20

... prior to incorporation of the three modified oxygen atoms and a cobalt atom into the model structure (C) Fo–Fc electron density map (6 r) constructed prior to incorporation of a cobalt atom into ... atoms of the side-chains of aCys111 and aCys113 were moved to the outside, and the cobalt atom was also slightly moved (Fig 4) As a result, the distances between the cobalt atom and its ligands ... mutated residue in the aT109S and aY114T structures, and a cobalt atom and three oxygen atoms in the apoenzyme and aY114T structures Several rounds of refinement and model Ó FEBS 2003 432 A Miyanaga...

Ngày tải lên: 16/03/2014, 16:20

... from the excretory-secretory (ES) products of the nematode and has a high affinity for fatty acids and retinoids [8] This finding applies to all the representatives of the family and suggests an ... J Photochem Photobiol B Biol 17, 155–161 Stone KL & Williams KR (1986) High-performance liquid chromatographic peptide mapping and amino acid analysis in the sub-nanomole range J Chromatogr 359, ... proteins offload their ligands to membranes: differential mechanisms of fatty acid transfer by the ABA-1 polyprotein allergen and Ov-FAR-1 proteins of nematodes and Sj-FABPc of schistosomes Biochemistry...

Ngày tải lên: 16/03/2014, 18:20

... correspond to helix and helix of the second EcCM protomer; in *YpCM, the disulfide bond is between the third residue of the mature protomer and the bottom of helix of the second EcCM protomer, Cys33 and ... the TSA onto 90-MtCM to see the TSA in the active site of 90-MtCM (Figs and 6) As the residues corresponding to Ser84 and Gln88 of EcCM are absent in 90-MtCM, chorismate is unlikely to be as well ... EDTA, 0.1 mg BSAÆmL)1, and 10 mM b-mercaptoethanol Chorismate concentrations were varied from 0.25 to mM Assays were performed at 37 °C for [34], and the reaction was stopped with 0.3 mL of M...

Ngày tải lên: 17/03/2014, 17:20

... regulatory relationships and associating the regulatory time delay with every “parentchild” (i.e., regulator-target) pair [13] The data set was partitioned into parent set (the regulators) and ... Data To demonstrate the difference between the SISO and MISO models, we first apply only SISO to network prediction on the artificial data The two regulators, R1 and R2, are expected to connect to ... G1 and late G1 phase gene regulation [28, 29] The three TFs constitute two transcription factor complexes: SBF (Swi4 and Swi6), and MBF (Swi6 and Mbp1) SBF and MBF control over 50% of the total...

Ngày tải lên: 21/06/2014, 20:20

... is suggested to contribute to tolerance through maintenance of the CD4+CD25+ regulatory T cell population [35] Therefore, we have presented a strategy to identify smalleffect QTL and search for ... 2, 1, and potential candidate genes within the confidence intervals of loci 1, 2, 3, 5, and 8, respectively, while no candidate gene was identified for loci and Table summarises the 21 candidate ... and KB performed the animal experiments DK and HJT performed gene expression profiling experiments XY and DK performed the bioinformatic analysis SI conceived and designed the experiment XY and...

Ngày tải lên: 09/08/2014, 10:20

... compared to the wild type, may provide valuable insights into elucidating and understanding the biochemical and structural basis of the abscission process [26] Such mutants have been described and ... magnification of the ALZ in (M) and (N), respectively Seeds in the first (most mature) pod and close to pea stock are designated as 1.1 The youngest pod and close to the pea stock is designated as 8.1 ... prerequisite prior to abscission as in tobacco, tomato and several other solanaceous genera [50] Like pea, these plants have a visible AZ long before abscission is initiated and not shows cell...

Ngày tải lên: 12/08/2014, 03:20

... applicable to any data, regardless of experimental source The database is publicly available online, and experimentalists are encouraged to submit their data for storage and display Results Handling and ... are expected to be accurate Phosphorylatable residues are not always accessible to solvent Next, we wished to assess the accessibility of phosphorylatable residues to solvent and thus to protein ... in some cases additional co-factors/effectors may be required to actively extract and present the residues to the phosphorylating enzymes Finally, although this structural study has revealed some...

Ngày tải lên: 14/08/2014, 07:21

... http://genomebiology.com/2005/6/10/R82 and tumor necrosis factor receptor associated factor homology (MATH)-BTB and Skp1 proteins In Arabidopsis, there are 21 BTB-nonphototropic hypocotyl (NPH)3 proteins, which appear to be ... relative to the BTB domain due to the structurally disordered central region Further work will be required to understand the structure and function of BTB-ZF proteins in SCF-like complexes Stogios ... Hoshino H, Muto A, Sato K, Morita M, Hayashi N, Yamamoto M, Igarashi K: A combinatorial code for gene expression generated by transcription factor Bach2 and MAZR (MAZ-related factor) through...

Ngày tải lên: 14/08/2014, 14:22

... scMis12Mtw1 and scNdc80Hec1, kinetochore proteins first identified in yeast and subsequently shown to have human orthologs (hsMis12 and hsNdc80Hec1) that localize to kinetochores and play a role ... spindle assembly From yeast to humans, only one or two of the six to ten kinetochore MAPs and motors are specific to kinetochores CENP-A functions in most organisms to determine CEN location without ... domains of checkpoint and regulatory proteins and motor domains of kinesins), blocks of conserved sequence not correspond to recognizable functional domains This stands in contrast to the situation...

Ngày tải lên: 14/08/2014, 16:21

... ice and processed within d of capture For each fish, fork length (FL) and total length (TL) were measured to the nearest millimeter and total mass was recorded to the nearest gram After a standardized ... field and laboratory assistance L Flagg provided sampling assistance and historical perspectives on the fishery A Pershing and N Record coded algorithms to correct for image distortion T Bartlett and ... plastic grid Fifteen landmarks were used on each fish, and 10 of the landmarks were marked by pins prior to photo documentation (Armstrong and Cadrin 2001) From the 15 landmarks, 31 measurements...

Ngày tải lên: 04/09/2015, 08:12

... enables to proceed the structural analysis, in order to identifl potentially critical regions In order to reduce the computational cost of the analysis and to minimize the input data, the structural ... Direct your orders to SAE Customer Sales and Satlsfactlon Department Quantity reprint rates can be obtalned from the Customer Sales and Satisfaction Department TOrequest permissionto reprint a techn~calpaper ... components analysis techniques in the aerospace industry in a first moment and, more recently, in the automotive industry Among them are the analysis methods like the finite element method and time...

Ngày tải lên: 16/09/2016, 17:13

... technique that can be used to characterize and identify binding [21–23] It can also be used to identify the binding epitope of ligands to a protein receptor [21] This feature can NMR of MUC-1 glycopeptides ... corresponds to a ligand to protein ratio of 20 : For STD experiments the ligand to protein ratio was raised to 150 : (2.88 lmol, 4.8 mM PDT(O-a-D-GalNAc)RP) Peptide or glycopeptide were added to the ... b protons Assuming a binding mode as found in the X-ray structure analysis the closest distance of a protein ˚ proton to the Asp2-b/b¢ proton is 2.7 and 3.4 A, respectively This relay proton contributes...

Ngày tải lên: 21/02/2014, 15:20

... required to identify $9.6B in FY99 to DLA, A lot of detective work required to identify $9.6B in FY99 to DLA, GSA, TRANSCOM, DFAS, DISA GSA, TRANSCOM, DFAS, DISA RAND unable to identify and quantify ... factors, etc economic factors, etc The application of analytical and benchmarking tools The application of analytical and benchmarking tools that link these analyses to help identify key indicators ... suppliers and the supply base In sum, a spend analysis integrates internal spend data and external supplier and market data and applies analytical and benchmarking techniques to help identify risks and...

Ngày tải lên: 06/03/2014, 16:20

... tree No response of stomata to air vapour pressure deficit No response of stomata to radiation condition No response of stomata to predawn potential No response of stomata to climatic conditions ... time, and such models remain close to production tables On the other hand, process-based models are forced to take into account the geometry of trees, that is to say their architecture, in order to ... gw, f1, f2 and f3 describing the stomatal response to air vapour pressure deficit (D), total intercepted PAR per leaf area unit and predawn water potential respectively The inertia of stomatal reaction...

Ngày tải lên: 08/08/2014, 14:22

... differential expression analysis, we have attempted to identify and segregate expression profiles specifically (preferentially) relevant to the events related to male gametophyte development These ... experiment for MA stage WDB and JAA helped in the data analysis and preparation of the manuscript SK conceptualized and designed the experiments, contributed to the data analysis and manuscript writing ... probe-sets correspond to annotated genes, and in some instances more than one probe-set corresponds to annotated genes Therefore, in order to identify the unique probe-sets that correspond to annotated...

Ngày tải lên: 11/08/2014, 11:20

... sliding-window-based approach was used to systematically identify intergenic TARs (see Materials and methods) Through this analysis, 574 and 522 intergenic TARs in indica and japonica were identified from ... Cloning (see followinganalysis of japonica chromosome 10 UG models and intergenic TARs Figure and sequence page) Cloning and sequence analysis of japonica chromosome 10 UG models and intergenic TARs ... cloned PCR products were aligned back to japonica chromosome 10 using BLAT [62] to confirm their identify and to map their corresponding gene structure RNA gel-blot analysis of intergenic TARs was...

Ngày tải lên: 14/08/2014, 14:21

Ngày tải lên: 14/08/2014, 14:21

... proteins and the SAMdependent MTases is the insertion of a 7th antiparallel β-strand into the sheet between the strands The overall topology of the strands in these MTases is 3214576 (Martin and McMillan ... in the TIM barrel and the difference is reflected in the curvature of the sheet and orientation of strands (Kozbial and Mushegian 2005) All these structural variations lead to a change in the ... importance to answer some of the unanswered questions with regard to the conversion of triostin A to echinomycin and to unravel the mechanism of action of this unique enzyme, leading to the objectives...

Ngày tải lên: 04/10/2015, 10:23