immunization infection and analysis of mice
Báo cáo sinh học: " Genetic characterisation of the recent foot-and-mouth disease virus subtype A/IRN/2005" pptx
Ngày tải lên :
18/06/2014, 18:20
... Bayesian1phylogenetic analysis of the complete coding sequence of the A/IRN/2005 sublineage (red) and related published Figure sequences (black) Bayesian phylogenetic analysis of the complete coding sequence of ... Bayesian4phylogenetic analysis of the 1D genome region of the A/IRN/2005 sublineage (red) and related published sequences Figure (black) Bayesian phylogenetic analysis of the 1D genome region of the A/IRN/2005 ... Control of Foot -and- Mouth Disease (EUFMD) International Control of Foot -and- Mouth Disease: Tools, trends and perspectives in Cyprus, 16–20 October 2006 European Commission for the Control of Foot -and- Mouth...
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Báo cáo hóa học: " Genetic characterisation of the recent foot-and-mouth disease virus subtype A/IRN/2005" ppt
Ngày tải lên :
20/06/2014, 01:20
... Bayesian1phylogenetic analysis of the complete coding sequence of the A/IRN/2005 sublineage (red) and related published Figure sequences (black) Bayesian phylogenetic analysis of the complete coding sequence of ... Bayesian4phylogenetic analysis of the 1D genome region of the A/IRN/2005 sublineage (red) and related published sequences Figure (black) Bayesian phylogenetic analysis of the 1D genome region of the A/IRN/2005 ... Control of Foot -and- Mouth Disease (EUFMD) International Control of Foot -and- Mouth Disease: Tools, trends and perspectives in Cyprus, 16–20 October 2006 European Commission for the Control of Foot -and- Mouth...
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Báo cáo y học: " Dideoxynucleoside HIV reverse transcriptase inhibitors and drug-related hepatotoxicity: a case report" pot
Ngày tải lên :
11/08/2014, 10:22
... data and revised the manuscript LB and MG analyzed the liver biopsy and revised the manuscript CT conceived the study and participated in the drafting of the manuscript All authors read and approved ... regardless of the "background" effect of chronic hepatitis co-infections The mechanism of d4T toxicity may have been mediated by liver steatosis, which has already been associated with d4T and other ... occur in the event of hepatotoxicity Conclusion This case report underlines the importance of d4T as a possible causative agent of hepatotoxicity, even in the absence of other signs of mitochondrial...
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Báo cáo khoa học: Alizarine derivatives as new dual inhibitors of the HIV-1 reverse transcriptase-associated DNA polymerase and RNase H activities effective also on the RNase H activity of non-nucleoside resistant reverse transcriptases pot
Ngày tải lên :
22/03/2014, 16:20
... presence of different concentrations of efavirenz and in the absence () or presence of 1.9 lM (s), 3.7 lM (.) and 7.5 lM (4) K-49 (C) YonetaniTheorell plot of the interaction of KNA-53 and efavirenz ... the presence of increasing concentrations of one of the two AQ derivatives and efavirenz, and analyzed using the YonetaniTheorell plot, the slope and intercepts of the two plots of v versus ... incubated in the presence of different concentrations of K-49 and in the absence () or presence of lM (.) or 10 lM ( ) RDS1643 (B) YonetaniTheorell plot of the interaction of K-49 and efavirenz on the...
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Báo cáo Y học: The processivity and fidelity of DNA synthesis exhibited by the reverse transcriptase of bovine leukemia virus pot
Ngày tải lên :
31/03/2014, 21:21
... potent inhibitors of other RTs such as that of HIV [12] In the present study we have extended the investigation of BLV RT by an in vitro analysis of the processivity and the fidelity of DNA synthesis ... the ability of BLV RT to misincorporate nucleotides at the 3¢ end of the growing DNA strand and the further extending of the preformed mismatches) MATERIALS AND METHODS Recombinant RTs and DNA polymerase ... (depending on activity) and Ó FEBS 2002 Processivity and fidelity of BLV RT (Eur J Biochem 269) 861 Table Quantitative analysis of DNA primer-extension and relative processivity of DNA synthesis The...
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Báo cáo sinh học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" pdf
Ngày tải lên :
18/06/2014, 18:20
... reaction contained µl of cDNA from the RT reaction, µl of 10× PCR Buffer, 1.5 mM MgCl2, µl of 10 mM each of dATP, dCTP, dGTP, and dTTP per reaction, µM each of primers and 2.5 units of Platinum Taq ... Sub-Programme (Dept of Agriculture and Food, Republic of Ireland) Thank you to Mr Noel Shanaghy and Mrs Breda Doody of Waterford Regional Hospital, Mr Eddie Beggan of Limerick Regional Hospital and Dr Jim ... number concentration of × 107 – × 101 molecules are shown from left to right (B) Standard curve of GII assay R2 1.00 and a slope of -3.7 was obtained (C) Melting curve of GII standards showing melting...
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Báo cáo sinh học: " Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR" pdf
Ngày tải lên :
19/06/2014, 08:20
... real-time PCR and RTPCR offers significant advantages over standard PCR and RT-PCR for detection of DNA or RNA in terms of reduced sample handling, the time required for analysis and analytical ... elimination of carry-over contamination has led several manufacturers of commercial PCR and RT-PCR reagents to substitute dUTP in the reaction mixture in place of dTTP and in the case of some manufacturers ... Simultaneous detection of viral RNA and contaminating DNA Simultaneous detection of viral RNA and contaminating DNA Serial ten-fold dilutions of viral RNA with approximately 1,000 copies of heterologous...
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Báo cáo hóa học: " Development of a real-time RT-PCR and Reverse Line probe Hybridisation assay for the routine detection and genotyping of Noroviruses in Ireland" ppt
Ngày tải lên :
20/06/2014, 01:20
... reaction contained µl of cDNA from the RT reaction, µl of 10× PCR Buffer, 1.5 mM MgCl2, µl of 10 mM each of dATP, dCTP, dGTP, and dTTP per reaction, µM each of primers and 2.5 units of Platinum Taq ... Sub-Programme (Dept of Agriculture and Food, Republic of Ireland) Thank you to Mr Noel Shanaghy and Mrs Breda Doody of Waterford Regional Hospital, Mr Eddie Beggan of Limerick Regional Hospital and Dr Jim ... number concentration of × 107 – × 101 molecules are shown from left to right (B) Standard curve of GII assay R2 1.00 and a slope of -3.7 was obtained (C) Melting curve of GII standards showing melting...
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Báo cáo hóa học: " Sargassum fusiforme fraction is a potent and specific inhibitor of HIV-1 fusion and reverse transcriptase" pot
Ngày tải lên :
20/06/2014, 01:20
... potent inhibitor of HIV-1 infection, which at a concentration of mg/ml lowered viral infection by up to 80% in a variety of primary cells and cell lines, and for a prolonged period of time [2] To ... multiplicity of infection (moi) of 0.01 for 1.5 h, washed times, and returned to culture with the same concentration of each treatment, for the duration of the experiment (A) On day after infection, ... entry into cells consists of two distinct steps of 1) virus binding to the cellular receptor and coreceptor, which is followed by 2) fusion of the viral and cellular Page of (page number not for...
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báo cáo hóa học:" Quantitative assessment of the effect of uracil-DNA glycosylase on amplicon DNA degradation and RNA amplification in reverse transcription-PCR" ppt
Ngày tải lên :
20/06/2014, 04:20
... real-time PCR and RTPCR offers significant advantages over standard PCR and RT-PCR for detection of DNA or RNA in terms of reduced sample handling, the time required for analysis and analytical ... elimination of carry-over contamination has led several manufacturers of commercial PCR and RT-PCR reagents to substitute dUTP in the reaction mixture in place of dTTP and in the case of some manufacturers ... Simultaneous detection of viral RNA and contaminating DNA Simultaneous detection of viral RNA and contaminating DNA Serial ten-fold dilutions of viral RNA with approximately 1,000 copies of heterologous...
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báo cáo hóa học:" Substitutions in the Reverse Transcriptase and Protease Genes of HIV-1 Subtype B in Untreated Individuals and Patients Treated With Antiretroviral Drugs" potx
Ngày tải lên :
20/06/2014, 08:20
... of APOBEC3G is inhibited by the Vif protein.[5,7,8] In the absence of Vif, the synthesis of the negative strand of DNA can result in the insertion of a uracil as a result of the deamination of ... standard error of the mean.) stavudine only occurred in 4% of patients treated with this drug.[10] Of note, some of the common resistance mutations that are easily selected by drugs in vivo and ... PIs and NRTIs, both NNRTIs and NRTIs, or only NRTIs The different regions of RT and PR were analyzed based on the types of drugs employed in therapy Among major resistance mutations, 47% of PItreated...
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Báo cáo khoa học: "Immunohistochemical expression of h-telomerase reverse transcriptase in canine and feline meningiomas" pptx
Ngày tải lên :
07/08/2014, 20:23
... sesac ruof ylno ;)52 fo tuo 12 :puorg L( samoigninem fo ytirojam tsav eht ni ytivitca citotim wol yrev a etaicerppa ot lufesu saw )2 giF( gniniats eulb enidiulot deifidoM stac ro sgod rof epytotsih ... eht ni ;detceted saw langis on ,52 fo tuo snemiceps eerht nI )1 elbaT( )C-A1 giF( )sesac 2( sllec lailehtogninem citsalpoen fo msalpotyc eht ni dnuof yllanoisacco osla saw dna ,suloelcun eht ylbaton ... deniatsretnuoc neht erew snoitceS negomorhc sa )nim rof %40.0( enidiznebonimaid dna )sdnalrehteN ,okaD ;tiK BASL( xelpmoc esadixorep -nitoib-nidivatperts a yb dewollof ,Co4 ta thginrevo la te iloirdnaM anaicuL...
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Báo cáo y học: "Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction" potx
Ngày tải lên :
09/08/2014, 01:24
... -2, and -3 in the synovium synthase-1, 2, and -3 and (OA) levels of the messages (RA) Relative expressionand rheumatoid arthritis for hyaluronanof knees in 2, and -3 and hyaluronidase-1, -2, and ... HPLC analysis for the measurement of the average molecular weight of HMW hyaluronan Analysis of message expression by quantitative realtime RT-PCR Message expression in the synovium of knees and ... Expression profile of hyaluronan synthase isoform messages Expressed messages for all three HAS isoforms were detected in all synovial samples The expression of the messages for HAS-1 and HAS-2...
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Báo cáo y học: ": Prevalence of reverse transcriptase and protease mutations associated with antiretroviral drug resistance among drug-naïve HIV-1 infected pregnant women in Kagera and Kilimanjaro regions, Tanzania" pptx
Ngày tải lên :
10/08/2014, 05:21
... Regional Medical Officers in Kagera and Kilimanjaro regions, and the Medical Officers of the Municipal Councils in Moshi and Bukoba The Institute of Microbiology, Rikshospitalet, University of Oslo provided ... laboratory analysis, and KIK and FM participated in laboratory analysis, FM and CHH participated in study design, interpretation of results All authors contributed to the writing, read and approved ... out-group for phylogenetic analysis Page of (page number not for citation purposes) AIDS Research and Therapy 2008, 5:13 Reports from Uganda [23,24], Rwanda [24], Mozambique [25] and Zambia [26] indicated...
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Báo cáo y học: " Efficacy, safety and pharmacokinetic of once-daily boosted saquinavir (1500/100 mg) together with 2 nucleos(t)ide reverse transcriptase inhibitors in real life: a multicentre prospective study" ppt
Ngày tải lên :
10/08/2014, 05:21
... open-label characteristics of the study, the heterogeneity of the analyzed population and the lack of available genotypic resistance tests in some of the patients at baseline and after virological ... under 100 ng/ml, and of them had a satisfactory virological response No correlations were found between SQV levels and either weight, gender or the presence of chronic hepatitis and/ or cirrhosis ... OT analysis, the efficacy raised to 90.4%, with similar results regardless of baseline VL and CD4 counts The presence of more adherent patients in the “simplification” group may be the cause of...
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Báo cáo y học: " A Leu to Ile but not Leu to Val change at HIV-1 reverse transcriptase codon 74 in the background of K65R mutation leads to an increased processivity of K65R+L74I enzyme and a replication competent virus" pptx
Ngày tải lên :
11/08/2014, 21:21
... addition of 50 μCi of [a-32P] dTTP/ml and 50-fold excess of trap [poly (rC)-oligo (dG)] The reactions were terminated after 180 by placing the tubes in ice slurry and addition of the equal volume of ... amount of wild type and mutated (AAA/AGA) cDNA, and generated regression line between ratios of peak heights for ‘A’ and ‘G’ nucleotides (A/G) and cDNA concentrations (Figure 3) The percentages of ... (bands 19-24), 0.010 (bands 25-30) and 0.023 (bands 31-36) (Figure and Figure 6) Thus, L®I change at RT codon 74 resulted in an increased processivity of RT with K65R mutation Our analyses of...
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Báo cáo y học: " A duplex real-time reverse transcriptase polymerase chain reaction assay for detecting western equine and eastern equine encephalitis viruses?" docx
Ngày tải lên :
12/08/2014, 01:22
... a robust and valuable tool for highly sensitive and specific detection of WEEV and EEEV infection Page of 11 Lambert AJ, Martin DA, Lanciotti RS: Detection of North American Eastern and Western ... grinded, and the RNA was extracted and applied with the duplex real-time RT-PCR assay Results Sensitivity and specificity analysis of the duplex system Figure and Table show the sensitivity of the ... EEEV infection exits in China With the globalization of international trade, the rapid and free movement of large amounts of people, animals, food and feed products has created the risk of a...
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Báo cáo y học: " The RNA binding protein HuR does not interact directly with HIV-1 reverse transcriptase and does not affect reverse transcription in vitro" doc
Ngày tải lên :
12/08/2014, 23:23
... and biophysical characterization of GSTand NusA-HuR (A) Analysis of the expression and purification of GSTHuR by SDS PAGE Lanes and illustrate total protein and total soluble protein after E ... solution and the molecular masses of NusA-HuR and NusARRM were determined as 82.2 and 68.9 kDa, respectively These values are within ± 15% of the predicted masses of 99 kDa for NusA-HuR and 73 ... Details 1Department of Structural Biology, Division of Infectious Diseases, University of Pittsburgh School of Medicine, Pittsburgh, PA 15261, USA and 2Department of Medicine, Division of Infectious...
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Báo cáo y học: "Comparative biochemical analysis of recombinant reverse transcriptase enzymes of HIV-1 subtype B and subtype " ppt
Ngày tải lên :
13/08/2014, 01:20
... inhibited by NRTIs and NNRTIs to a similar extent These findings are supportive of the use of recombinant RTs of either subtype for enzyme analysis, drug design, and for studying mechanisms of drug resistance ... concentration mg/ml) and incubated at 37°C for 10 min; μl of reaction mixture were removed and mixed with μl of formamide sample buffer (90% formamide, 10 mM EDTA, and 0.1% each of xylene cyanol and bromophenol ... in the absence of trap to ensure that similar amounts of enzyme activities were present in the reactions The sizes of some fragments of the standard are indicated on the left side of the panel...
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Báo cáo y học: "The evolution of HIV-1 reverse transcriptase in route to acquisition of Q151M multi-drug resistance is complex and involves mutations in multiple domains" ppt
Ngày tải lên :
13/08/2014, 01:20
... and genotypic testing For patient P66, six samples were collected at 0, 4, 10, 17, 28, and 37 months after initiation of therapy; four of which were available for viral load testing and SGS analysis ... HIV-1 virus and whether the C-terminal region of RT played a role in the emergence of the Q151M MDR complex The emergence and presence of mutations in DNA pol domain, connection subdomain and RNase ... capacity (% of 4-month RT) 4- 37-Pol-Cn Figure NRTI susceptibilities and replicative capacity associated with RT domains of patient P66 (A) Schematic representation of fulllength and chimeras of subtype...
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