... dichloromethane and distilled water after filtration by a glass fiber filter (Whatman GF/B, previously rinsed with MeOH and dried) The SPE column was eluted by dichloromethane, and then the samples ... Gangjung 5/02 10/ 02 3/03 9/03 Goryung 5/02 10/ 02 3/03 9/03 Jeokpo 5/02 10/ 02 3/03 9/03 Namji 5/02 10/ 02 3/03 9/03 Samrangjin 5/02 10/ 02 3/03 Gumho River 5/02 10/ 02 3/03 9/03 Hwang River 5/02 10/ 02 3/03 ... Perspectives, 104 (10) , 108 4 -108 9 C Desbrow, E.J Routledge, G.C Brighty, J.P Sumpter, and M Waldock (1998) Identification strogenic chemicals in STW effluent Chemical fractionation and In Vitro...
... IFN-γ, TNF-α, IL- 2 and IL- 4 and are important in controlling the equilibrium between Th1 and Th2 responses [25] Deficiency of these cells provokes poorly organised cellular infiltrates suggesting ... humoral immune response, observed by the detection of IgG antibodies, between day 10and 14 after oral immunisation (figure 1) Subjects and 6, who received more than one dose of oral vaccine and ... (represented by IFN-γ) and Th2 (represented by IL- 4) responses All subjects showed a lymphoproliferative response to PPD Rt48 although the magnitude of stimulation differed (figures and 4) Volunteers...
... release of both IL- 6 and IL- 10 was significantly less than at pHo 7.0 or 7.4 However, IL- 10 release was reduced to a far greater extent than was IL- 6, and thus the ratio of IL- 6 to IL- 10 increased ... (pH 6.5) NO ↓ ↓↓ ↑ – iNOS mRNA ↓ ↓↓ ↑ ↑↑ IL- 6 ↓ ↓↓ – ↓ IL- 6 mRNA ↓ ↓↓ – ↓ ↓ ↓↓ ↓ ↓↓↓ ↓↓ ↓↓ – – IL- 6 : IL- 10 ratio – – – ↑↑ NF-κB ↓ ↓↓ ↑ ↓ IL- 10 IL- 10 mRNA IL, interleukin; iNOS, inducible nitric ... HCl and stimulated the cells with 10 ng/ml LPS (Escherichia coli 0111:B4) for 24 hours Acidic medium itself barely affected the release of inflammatory mediators, including NO, IL- 6, and IL- 10...
... culture and tested in ELISA for (a) IFN-γ and (b) IL- 10 The results are expressed as pg/ml (mean + standard error of the mean) The difference in the level of IL- 10 but not of IFN-γ in response ... changes in the immuneresponse to Bhsp65 Furthermore, both the cellmediated and the antibody responses to Bhsp65 were affected AA is driven by pro-inflammatory cytokines (IFN-γ and Page of 10 (page ... contribute to the protection against AA by modulating antigen processing and presentation [43] and, thereby, facilitating the induction of the immuneresponse to one or more of the regulatory...
... (TNF-a), interleukin (IL) -6, IL- 1receptor antagonist (IL1 RA), and IL- 10 were measured in samples taken at baseline and at 30, 60, 120, 240, and 480 minutes after LPS administration and subsequently ... (Table 2) Plasma concentrations of pro- and anti-inflammatory cytokines (TNF-a, IL- 6, IL- 10, and IL1 RA) are shown in Figure Thus, caffeine administration and the presence of the 34C > T variant ... 1200 1200 P
... described in detail in both the experimental procedures (Ki of cyanidin, bilirubin, biliverdin, section B) and in Table (Kd of the complexes of bilitranslocase with bilirubin, nicotinic acid and cyanidin ... inhibitors were: 52.4 lm cyanidin 3-glucoside; 24.6 and 41 lm cyanidin; 100 nm bilirubin and 100 nm biliverdin Under the conditions of the assay, bilirubin is freely soluble in the buffer [58] The ... 5000 dilution, followed by the addition of the chemiluminescent substrate ECL (Amersham Biosciences) Negative controls were obtained by using preimmune sera instead of the primary antibodies Bilitranslocase...
... Escherichia coil in retail fresh seafood, beef, lamb, pork and poultry from grocery stores in Seattle, Washington Applied and Environmental Microbiology, 60, 103 8 – 104 0 Mead G, G Hudson, W, R and Hinton ... than 1% for many years and the organism are rarely found in retail samples due to rigorous surveillance andcontrol programmes that are relatively costly to operate (Persson and Jendteg, 1992) Food ... Campylobacter and 16 on Listeria, including completed and on-going studies in both developed and developing countries, as well as those undertaken by FAO/WHO on Salmonealla and Campylobacter in broilers...
... for min, and separated by SDS ⁄ PAGE followed by silver staining (B) Purified proHP8Xa (75 ng) was analyzed by SDS ⁄ PAGE under reducing conditions followed by silver staining The sizes and positions ... NaCl, 10 mm imidazole, pH 8.0) Cells were lysed by sonication, and a cleared lysate was obtained by centrifugation at 10 000 g for 30 The soluble Spatzle-C108 in the super¨ natant was purified by ... described above, diluted in 10 mL of 20 mm Tris ⁄ HCl and 200 mm NaCl (pH 8.0), and then incubated with 100 lL of Ni2+–nitrilotriacetic acid agarose at °C for h The Ni2+–nitrilotriacetic acid...
... incubation were taken at 1, and h, and extracellular and intracellular media were separated by centrifugation and analysed in parallel by 1H NMR spectroscopy andby TLC Under these conditions, ... water and the cells were broken by three successive freeze ⁄ thawing procedures Cell debris and cellulose were pelleted by centrifugation (10 000 g, 10 min) and the supernatant was assimilated ... cellulose and with cellobiose (the main product of cellulolysis), and with a mixture of glucose and cellobiose Cell extracts and extracellular media were analysed by 2D NMR spectroscopy andby TLC...
... bovine serum and 100 UÆmL)1 penicilin and streptomycin at 310 K in a 5% CO2 atmosphere All experiments were performed with a cell density of % · 105 cells per mL Preparation of FeC-Tf and ferric-saturated ... Fe(nitrilotriacetate)2 to apo-TF and incubated the solution at 310 K for 30 The solution was then passed through Sephadex G25 column (1 · 10 cm) to remove the low molecular mass ligands (e.g nitrilotriacetate), ... lM apo-Tf in mM NaHCO3 and10 mM Hepes buffer at pH 7.4 and 310 K) after addition of various amounts of molar ratios of Yb3+ (as YbCl3) The two absorption bands at 242 and 295 nm are indicative...
... on the emotion of the building and the metaphorical relationship man has with the contents of the building The example of the National Archives andits meaning as a building is the first major ... Union (1 910) , where Cret describes his goal of the building to be "the visible expression of the ideals of unity, solidarity, and amity to which the Union is dedicated." The building itself is ... is not too grand in size to impose a visitor, yet his subtle use of pilasters and a characteristic three-arch entryway, implies a great deal monumentality and significance to the building Another...
... reactions were terminated by adding Laemmli sample buffer and heating samples at 100 °C for 10 Aliquots were separated by SDS ⁄ PAGE, and after staining with 0.1% Coomassie Brilliant Blue the gels ... periods (A) or 10 lM LY294002 (LY), 50 nM rapamycin, 10 lM SB203580 or mM GF109203X followed by insulin (B) Following cell lysis, HA-tagged TSSK3 was isolated by immunoprecipitation and TSSK3 activity ... Escherichia coli, and purified by one-step affinity chromatography Two control peptides were cloned and purified in parallel, a peptide not phosphorylated by TSSK3 or PKA (peptideNEG) and a peptide...
... related work, and Section presents the pattern clustering and labeling algorithm Section describes the corpora we used and the algorithm’s parameters in detail Sections and Turney and Littman (2005) ... (Davidov and Rappoport, 2006; Widdows and Dorow, 2002) and meronymy (Berland and Charniak, 1999; Girju et al., 2006) Since named entities are very important in NLP, many studies define and discover ... demonstrate in our bilingual evaluation 2.3 Evaluation Method Evaluation for hypernymy and synonymy usually uses WordNet (Lin and Pantel, 2002; Widdows and Dorow, 2002; Davidov and Rappoport, 2006)...
... animals were killed by cervical dislocation, and tibialis anterior, gastrocnemius and soleus muscles were carefully dissected and kept on ice In fasted animals and the respective controls, one ... containing 0.1% Tween and hybridized with a : 100 0 diluted goat anti-rabbit peroxidase-labeled secondary antibody The signals were detected by chemiluminescence using a standard ECL kit and developed ... IV and a : 100 0 diluted goat anti-mouse peroxidase-labeled secondary antibody To compare the UCP3 signals, linear standard curves were constructed using increasing concentrations of the human and...
... dialogue, initiative -response- unit (IR) and move Dialogue, in our notation, is similar to conversation in LOKI, while IR-units resemble exchanges IR-units are recursive and, unlike LOKI, we allow ... Most of the cars consume iless than litre/10km, be more specific U8> 0.7 litre/10km "$9> Daihatsu Charade Fiat Uno Ford Fiesta Honda Civic Lancia Y10/A112 Mazda 323 Mitsubishi Colt Nissan Micra ... Natural-Language Understanding and a Pilot Implementation Research Report LiTH-IDA-R-89-22, Department of Computer and Information Science, Link6ping University Ahrenberg, Lars, Arne J6nsson & Nils Dahib~lck...
... of tumoral hexokinase and PFK-1 To understand why hexokinase retained a significant degree of control on glycolytic flux, despite its high over-expression, and why PFK-1 control became negligible, ... Fru(2,6)P2 was the most potent activator of PFK-1 and blocked the inhibition by ATP and citrate (Fig 2B and Table 6, and also [12]) Therefore, its kinetic properties predict that PFK-1 activity ... phosphofructokinase subunits and isozymes in ascites tumor cells andits original tissue, murine mammary gland FEBS Lett 409, 86–90 Denis C, Paris H & Murat JC (1986) Hormonal control of fructose-2,6-bisphosphate...
... =Mr;ligand Þnð0:4ÞRimmobilized ððdn=dcanalyte Þ=ðdn=dcligand ÞÞ ð2Þ where (dn ⁄ dcanalyte) and (dn ⁄ dcligand) are the refractive index increment for the analyte and ligand, respectively Similar ... obtained at 100 , 400, 100 0 and 3000 lM Ca2+ (B) The equilibriumbinding isotherms for Ca2+ (d), Mg2+ (s), MgATP (.) and Na+ (n) with a half-maximal response ([A]0.5) at 2.8 mM for Ca2+ and 9.6 mM ... (exposure time 10 min), and immobilized covalently to the hydrophilic carboxymethylated dextran matrix of a CM5 sensor chip by the standard primary amine coupling reaction as described by the manufacturer...
... tendency to decrease As for ac- 903 20 01'0, i i 101 102 i 10~ i 104 t i 105 106 107 #characters in learning corpus Figure 3: Relation between cross entropy and parsing accuracy curacy, there also is ... is represented by the class calculated from the POS of its last content word and that of its last function word The relation between bunsetsu, called dependency, is described by a stochastic ... grouped by the attribute defined as follows: attrib(b) (1) = qast(co.t(b)), last(f c(b)), Zast(sig.(b))), where the functions cont, f u n c and sign take a bun~etsu as their argument and return its...