immortalization of human mesenchymal stromal cells with telomerase and red fluorescence protein expression
Báo cáo y học: "Controlling Osteogenesis and Adipogenesis of Mesenchymal Stromal Cells by Regulating A Circadian Clock Protein with Laser Irradiation" pptx
Ngày tải lên :
08/08/2014, 17:20
... differentiate from mesenchymal stromal cells (MSCs) MSCs are multipotent cells that can replicate as undifferentiated cells and that have the potential to differentiate to lineages of mesenchymal tissues, ... levels of mCRY1 decreased after blue laser irradiation relative to non-irradiated cells (Fig 1C) Protein products of CRY act together with PER proteins to inhibit CRY and PER transcription and close ... center of plate well) were observed by fluorescence microscopy To evaluate osteogenesis, MSCs were rinsed three times with PBS and fixed with 4% formalin in PBS Fixed cells were incubated with...
- 8
- 281
- 0
Báo cáo y học: "Chordin knockdown enhances the osteogenic differentiation of human mesenchymal stem cells" pdf
Ngày tải lên :
09/08/2014, 10:23
... transcripts Expression of BMP-2 and chordinstem cells during osteogenic differentiation of human mesenchymal stem cells (a) Temporal progression of bone morphogenetic protein (BMP)-2 and chordin expression ... LV, Cheng SL: Regulation of bone matrix protein expression and induction of differentiation of human osteoblasts and human bone marrow stromal cells by bone morphogenetic protein- 2 J Cell Biochem ... using WST-1 assay at 3, and days of culture After and days, cells treated with chordin siRNA had decreased proliferation, as compared with untreated cells, and cells treated with transfection reagent...
- 9
- 404
- 0
Báo cáo y học: "Hypertrophy is induced during the in vitro chondrogenic differentiation of human mesenchymal stem cells by bone morphogenetic protein-2 and bone morphogenetic protein-4 gene transfer" pps
Ngày tải lên :
09/08/2014, 14:22
... differentiation of mesenchymal progenitor cells and to up regulate the levels of type II collagen and aggrecan in chondrocytes and chondroprogenitor cells [1,7-11] During development of the limbs, ... differentiation of cultured human mesenchymal stem cells from marrow Tissue Eng 1998, 4:415-428 35 Mueller MB, Tuan RS: Functional characterization of hypertrophy in chondrogenesis of human mesenchymal ... by red fluorescence, compared with the Ad.Luc transduced (Figure 5a) and untrans- Figure Immunohistochemical analyses for cartilage matrix proteins of MSC pellets after chondrogenic induction with...
- 15
- 830
- 0
Fibronectin and laminin promote differentiation of human mesenchymal stem cells into insulin producing cells through activating Akt and ERK pptx
Ngày tải lên :
10/08/2014, 05:21
... increases the expression of insulin and Glut2, and transduction with both siERK and siAkt decreases the expression of insulin and Glut2 (mean ± S.D.; *p < 0.05 and **p < 0.01 compared with the scrambled ... other hand, PD98059 decreased the activation of ERK both in cells treated with FN or LAM (Figure 5B and 5D) and increased the activation of Akt about 2.7-fold and 2.1fold compared to FN and LAM ... between the expression of these genes in cells treated with or without ECM We then quantified the gene expression of insulin and Glut2 RT-PCR revealed adding FN or LAM increased the expression of Insulin...
- 10
- 332
- 0
Engineered poly(l lactic acid) based nanofibers for osteogenic differentiation of human mesenchymal stem cells
Ngày tải lên :
09/09/2015, 10:07
... images of 2D (A and B), the outer surface of 3D (C and D) and the center of 3D (E and F) electrospun PLLA/Col nanofibrous scaffolds cultured with human MSCs in the Ost medium on day 28 Figures C and ... OPN expression between PLLA and TCPS In Figure b, OCN expression of human MSCs on the PLLA nanofibers was considerably lower than on the TCPS plates In Figures c and d, the expression levels of ... gene expression of human MSCs cultured on PLLA and Dexloaded PLLA scaffolds in the growth and Ost-Dex media after 14 and 21 days: (A) ALP, (B) OCN, (C) BSP, and (D) Cbfa1 Significant difference of...
- 272
- 258
- 0
Applying macromolecular crowding to promote the expansion and adipogenic differentiation of human mesenchymal stem cells in vitro; an effect of matrix reciprocity
Ngày tải lên :
11/09/2015, 09:16
... 1991 ;Expression of modified bFGF cDNAs in mammalian cells Ann N Y Acad Sci :149-160 120 Kolf CM, Cho E, Tuan RS 2007 ;Mesenchymal stromal cells Biology of adult mesenchymal stem cells: regulation of ... differentiation of human mesenchymal stem cells Exp Cell Res (1):22-37 101 Salasznyk RM, Klees RF, Boskey A, et al 2007;Activation of FAK is necessary for the osteogenic differentiation of human mesenchymal ... 1988;Marrow stromal stem cells J Cell Sci Suppl :63-76 123 Beresford JN 1989;Osteogenic stem cells and the stromal system of bone and marrow Clin Orthop Relat Res (240):270-280 124 Caplan AI 1991;Mesenchymal...
- 9
- 423
- 0
Analyzing the immunobiology of cultured mesenchymal stromal cells
Ngày tải lên :
15/09/2015, 22:09
... CELLS 1.3.1 Definition of mesenchymal stromal cells 1.3.2 Heterogeneity of mesenchymal stromal cells 10 1.3.3 Homing of mesenchymal stromal cells 14 1.3.3.1 Mechanism of MSC homing 15 1.3.3.2 ... effects of mesenchymal stromal cells 1.4 19 PROGRESS IN IMMUNOLOGICAL STUDY OF MESENCHYMAL STROMAL CELLS 20 1.4.1 Immunogenicity of mesenchymal stem cells 22 1.4.2 Immune modulation by mesenchymal stromal ... ARE STEM CELLS 1.2.1 Definition of stem cells 1.2.2 History of stem cells 1.2.3 Common characteristics of stem cells 1.2.4 Classification of stem cells 1.3 WHAT ARE MESENCHYMAL STROMAL CELLS 1.3.1...
- 182
- 480
- 0
Báo cáo khoa học: Reaction of human UMP-CMP kinase with natural and analog substrates docx
Ngày tải lên :
08/03/2014, 02:20
... column (Amersham Biosciences) (100 mg protein for mL of gel), washed with 50 mL of buer A and 10 mL of buer A containing 0.3 M NaCl, before elution of the protein with buer A containing M NaCl Further ... ovalbumin and chymotrypsinogen as markers The elution volume of UMP-CMP kinase indicated a Stokes radius of 2.8 nm, in agreement with a monomer species Expression and purication of human UMP-CMP ... groups with catalytic efciencies in the range of 107 M)1ặs)1 for UMP and CMP, and of 105 M)1ặs)1 for AraCMP, L-3TCMP and dCMP (Table 1) Careful characterization of the reaction catalysed by human...
- 7
- 384
- 0
báo cáo hóa học:" Highly efficient transduction of human plasmacytoid dendritic cells without phenotypic and functional maturation" docx
Ngày tải lên :
18/06/2014, 15:20
... incubated with allogeneic T -cells stained with CFSE After days of co-culture, percentages of CD3+ dividing T cells measured by flow cytometry were linearly correlated with the loss of CFSE fluorescence ... function of natural killer cells, B cells, T cells and myeloid DC [16] Human pDC not express lineage specific markers, but are characterized by the expression of HLA-DR, CD4, CD123, BDCA2 and BDCA4 ... and non-adherent cells in culture were harvested at day and transduced by LV-VSVG-PGK at an MOI of 18 TU/ml and at a fixed concentration of × 106 cells/ ml, in RPMI 1640 After hours at 37°C, cells...
- 12
- 374
- 0
Directed differentiation of human embryonic stem cells into haematopoietic and definitive endodermal lineages
Ngày tải lên :
04/10/2015, 16:03
... and Fraser 2005) However, unhindered analysis of developmental events was made possible with the isolation of embryonic stem cells Extensive investigation of the properties and capabilities of ... lining of the embryo consists of both VE and DE cells (not exclusively VE) (3) Absence of expression of VE-specific genes is due to down regulation and not cell migration (4) VE-derived cells ... Pancreas and diabetes The pancreas consists of Acinar cells which perform its exocrine functions and clusters of cells called Islets of Langerhans which perform its endocrine functions Acinar cells...
- 296
- 2K
- 0
báo cáo hóa học:" Effects of TGF-β1 and IGF-1 on proliferation of human nucleus pulposus cells in medium with different serum concentrations" pptx
Ngày tải lên :
20/06/2014, 00:20
... of growth factors on proliferation of human NP cells (1%FBS/F12) (n = 20) Dose-response effect of growth factors on proliferation of human NP cells (1%FBS/F12) (n = 20) Cells were incubated with ... significant increase compared to the control group after 3, 5, and days of culture Compared with the data of standard medium at the days, the proliferative percence of TGFβ1, IGF-1 and TGF-β1+IGF-1 on ... significant stimulating effect of TGF-β1 over doses of 1.0–500.0 μg/L on NP cells, and the maximum stimulus with 10 μg/L of the TGF-β1 reached a 72.4% increase compared with the standard medium The results...
- 11
- 605
- 0
Báo cáo y học: "Human palatine tonsil: a new potential tissue source of multipotent mesenchymal progenitor cells" pdf
Ngày tải lên :
09/08/2014, 10:23
... epitope profile of tonsil-derived mesenchymal progenitor cells (T-MPCs) (a) Immunofluorescence analysis of cell surface epitope profiles of T-MPCs and bone marrow-derived mesenchymal progenitor cells ... 105, and × 104 cells per well, in triplicate) were cultured in expansion medium for weeks, fixed with 10% formalin, and stained with Giemsa (SigmaAldrich), and colonies of fibroblast-like cells ... supplements, with controls including T-MPCs and BM-MPCs of the same passage maintained and cultured in expansion medium, and BM-MPCs cultured in adipogenic medium Morphological changes in BM-MPCs and...
- 12
- 359
- 0
Báo cáo y học: " Expression of parathyroid hormone-related protein during immortalization of human peripheral blood mononuclear cells by HTLV-1: Implications for transformation" pps
Ngày tải lên :
13/08/2014, 05:20
... up-regulated during immortalization of PBMCs with HTLV-1 To determine the temporal expression of PTHrP during HTLV-1 immortalization of PBMCs, PTHrP mRNA (Figure 2A) and protein (Figure 2B) expression ... factor in conjunction with other oncogenes The goal of this study was to investigate the expression of PTHrP, its receptor, and MIP-1α during the early stages of immortalization of human lymphocytes ... during immortalization of PBMCs with HTLV-1 infection PTHrP was markedly up-regulated during immortalization of PBMCs with HTLV-1 infection (A) PTHrP mRNA expression during immortalization of PBMCs...
- 12
- 199
- 0
Báo cáo y học: "Signatures of human regulatory T cells: an encounter with old friends and new" pdf
Ngày tải lên :
14/08/2014, 16:21
... characterization of human TReg cells by studying 350 TReg cell associated genes selected on the basis of whole-genome transcription data from human and mouse TReg cells Application of our nonredundant Human ... restimulation via their TCR (Figure 2) Coexpression of GATA3 and FOXP3, but the lack of T-bet, suggests similarities in the gene expression profiles of Th2 and TReg cells in humans refereed research This ... regulatory phenotype and the amount of Foxp3+ cells within MACS purified human CD4+CD25+ T cells (Additional data file 5); and a Word table describing the phenotype of contaminating cells within MACS...
- 18
- 389
- 0
Vascularised bone tissue engineering endothelial progenitor cells and human mesenchymal stem cells coculture in 3d honeycomb scaffolds and the effect of bi rotational bioreactor and hypoxic microenvironment 1
Ngày tải lên :
09/09/2015, 10:16
... my two supervisors Prof Teoh Swee-Hin and A/Prof Jerry Chan I would like to thank them for their constant sharing of knowledge and ideas, their patience and understanding, and the many hours they ... Teoh Coculture of human endothelial progenitor stem cells and bone marrow-derived human fetal mesenchymal stem cells potentiates osteogenesis through paracrine activity in vitro and induces neovasculogenesis ... Osteogenic Cells 46 2.8.2.2 Mesenchymal Stem Cells 48 2.8.2.3 Mesenchymal Stem Cells in Bone Tissue Engineering .49 2.8.2.4 Proposed Cell Type – Human Fetal Mesenchymal Stem Cells...
- 146
- 300
- 0
Báo cáo y học: "Low temperature tolerance of human embryonic stem cells"
Ngày tải lên :
31/10/2012, 16:57
... colony with distinct sharp boundaries together with strong expression of both SSEA-3 and TRA-1-81; (2) Grade B that was partially differentiated, with some areas of nonuniform cell morphology and ... Oberkochen, Germany) with an HBO-103 mercury lamp and filter sets for FITC, Cy3.5, Texas Red, Cy5, Aqua, and DAPI Images were captured, processed, and analyzed using ISIS mBAND/mFISH imaging software (MetaSystems ... carried out with telomere-specific PNA probe (5 μg/ml) labeled with Cy3 (red fluorescence under an excitation wavelength of 559 nm), and centromere-specific PNA probe (30 μg/ml ) labeled with FITC...
- 6
- 477
- 0
Tài liệu Báo cáo khoa học: Calcium-independent phospholipase A2 mediates proliferation of human promonocytic U937 cells pptx
Ngày tải lên :
18/02/2014, 17:20
... Number of cells × 105 iPLA2-VIA by treatment of cells with bromoenol lactone (BEL) [11,12] In INS-1 insulinoma cells, acute inhibition of iPLA2-VIA reduces the relatively high content of lysoPC of ... shows that treatment of the cells with BEL induced a significant accumulation of cells in G2 ⁄ M and S, and a concomitant decrease of cells in G1, with respect to untreated cells In contrast, pyrrophenone ... as the cells entered G1 and then increasing again as the cells approached and entered S phase The same pattern of variation of iPLA2 activity was detected whether the assay was conducted with mixed...
- 10
- 450
- 0
Báo cáo khoa học: Cloning, expression and interaction of human T-cell receptors with the bacterial superantigen SSA ppt
Ngày tải lên :
07/03/2014, 16:20
... subsets are expanded by interaction with SSA Some authors indicated clonal expansion of T -cells bearing human Vb3, Vb12, Vb17 and Vb19 [20] Others showed proliferation of human T -cells bearing ... linear NaCl gradient About 15 mg of purified protein per litre of culture medium was obtained Reduction and alkylation of SSA SSA after Ni–NTA purification was reduced with 10 mM DTT for h at 25 °C ... immunization with mgÆmL)1 of SSAia mixed with a volume of complete Freund’s adjuvant Boosts were administered on day 7, 14 and 28 Sera obtained on day 35 were diluted 10-fold and tested by ELISA and immunoblot...
- 9
- 485
- 0
Báo cáo Y học: Tyrosine sulfation and N-glycosylation of human heparin cofactor II from plasma and recombinant Chinese hamster ovary cells and their effects on heparin binding pot
Ngày tải lên :
08/03/2014, 22:20
... instrument Reduction, carboxamidomethylation and tryptic digestion of HCII from plasma and from recombinant CHO cells One to two nanomoles of the purified protein were reduced, carboxamidomethylated and ... properties of HCII Here, we report on the structure of posttranslational modifications of plasma-derived and recombinant human HCII from CHO cells and their effects on heparin binding MATERIALS AND METHODS ... were filled with lL of this solution The tip of the capillary was placed orthogonally in front of the entrance hole of a QTOF II mass spectrometer (Micromass, Manchester, England) equipped with a...
- 12
- 489
- 0
Báo cáo khoa học: Functional association of human Ki-1⁄57 with pre-mRNA splicing events docx
Ngày tải lên :
16/03/2014, 02:20
... lysates, immunoprecipitations, and preparation of cytoplasmic and nuclear fractions Human L540 and HEK293 cells and monkey COS-7 cells were cultivated under standard conditions as described FEBS ... particularity of the SMN protein is its demand for the presence of Arg ⁄ Gly-rich regions in most of its inter- Fig Localization of Ki-1 ⁄ 57 to nucleoli and splicing speckles HEK293 cells were ... colocalization of Ki-1 ⁄ 57 with SMN ⁄ GEMS in Adox-treated cells (Fig 7Biii) Despite the diffuse nuclear distribution of EGFP–Ki-1 ⁄ 57, the majority of the red GEMS spots coincide with spots of brighter...
- 14
- 369
- 0