immobilized metal ion affinity chromatography

... in binding the third metal ion is not conserved and the function of the third metal ion in MtSTP is unknown Recently, serine ⁄ threonine phosphorylation ⁄ dephosphorylation has been shown to ... below) In addition to the M1 and M2 metal ions, we observed an additional metal ion (M3) in two monomers of the asymmetric unit (monomers C and D; Fig 2A) M3 was also modeled as a Mg2+ ion, based ... of metal ligand interactions relevant to proteins II Angles at the metal atom, additional weak metal donor interactions Acta Crystallogr D 56, 857–867 36 Harding MM (1999) The geometry of metal ligand...

Ngày tải lên: 07/03/2014, 09:20

... N-terminal region is a novel ion selection site in the ZIP family of proteins Results Subcellular localization of TjZNT1 and TjZNT2 in yeast Initially, differences in subcellular localization between ... N-terminal regions of these proteins TjZNT2 possesses a 36 amino acid hydrophilic extension at the N-terminus (Fig 2), and has a second methionine at position 37 that is in a similar position to the ... modification of the N-terminal sequence directly affects the conformation of the ion- selectivity domain in ZIP transporters clustered with TjZNT2 As the N-terminal region contains no known metalbinding...

Ngày tải lên: 29/03/2014, 00:20

... used as the substrate High buffer concentrations and high divalent metal ion concentrations inhibited the enzyme activity Interestingly, the Table Purification of recombinant endolysin of bacteriophage ... represent the mean ± standard deviation (n ‡ 3) Fraction volume (mL) Crude extract Chromatography on Toyopearl 650M Chromatography on phosphocellulose Protein concentration (mgÆmL)1) Specific activity ... the catalytic function of endolysin requires the presence of ions of some metals whose phosphate salts are poorly soluble We supposed that the cations needed for endolysin functioning might be Ca2+,...

Ngày tải lên: 30/03/2014, 02:20

... the cation include the activation of water for the hydration reaction and ⁄ or the stabilization of the anion transition state In the latter case, if the substrate coordination to the metal ion ... Based on mechanistic consideration, interaction of the divalent cation with the C2-carbonyl oxygen is expected to stabilize the enolate anion transition state Interaction with only the C1-carboxyl, ... from crude extract (lane 1); preparation after anion exchange (lane 2); preparation after hydrophobic column chromatography (lane 3), preparation after gel filtration (lane 4) The molecular mass of...

Ngày tải lên: 30/03/2014, 15:20

... from the crystallization buffer Water molecules complete the coordination spheres of both metal ions The c-phosphate is held in place through coordination with one of the metal ions (Mn2, bond ˚ ... binding of the metal ions However, when binding of the ATP took place in solution, the process was accompanied by creation of metal- binding sites In other words, the binding of metal ions appears ... functional conformation Another factor that influences the positioning of the c-phosphate is the interaction of conserved Ser104 with one of the phosphate oxygens of the b-phosphate This interaction...

Ngày tải lên: 30/03/2014, 16:20

... Centrifuged affinity chromatography Affinity repulsion chromatography Affinity tails chromatography Theophilic chromatography Membrane-based affinity chromatography Weak affinity chromatography ... chromatography 10 Affinity partitioning 11 Filter affinity transfer chromatography 12 Affinity precipitation 13 14 15 16 17 18 19 20 21 22 23 24 Affinity density perturbation Perfusion affinity chromatography ... chromatography Lectin affinity chromatography Metal- chelate affinity chromatography Covalent affinity chromatography Affinity electrophoresis Affinity capillary electrophoresis Dye-ligand affinity chromatography...

Ngày tải lên: 10/04/2014, 22:17

... Nucleic Acids Affinity Purification 143 RNA Affinity Chromatography 145 Nehal Thakor and Martin Holcik Immobilized Metallic Ion Affinity Chromatography (IMAC) 165 Ion Metallic Affinity Chromatography ... Applications of Affinity Chromatography Chapter Affinity Chromatography: Principles and Applications Sameh Magdeldin and Annette Moser Chapter Affinity Interactions as a Tool for Protein Immobilization ... purification, but elution conditions are not as mild as for GST fusion proteins Simple purification Very mild Purification can be performed under denaturing elution conditions minimize risk of conditions...

Ngày tải lên: 28/06/2014, 14:20

... Introduction 67 5.2 Materials and Methods 70 5.3 Results and Discussion 72 5.4 Conclusion .79 CHAPTER CONCLUSIONS AND FUTURE DIRECTIONS 81 6.1 Conclusions ... 79 
 ix
 LIST OF ABREVIATIONS GFP Green Fluorescent Protein RFP Red Fluorescent Protein ε Extinction Coefficient λexcitation Excitation wavelength maxima λemission Emission wavelength maxima τ0 ... efficient detection due to DsRed-Monomers excitation and emission in the red region of the spectrum Protein-based affinity tags have also been shown to aid in the folding of the fusion partner,...

Ngày tải lên: 24/08/2014, 11:12

... increasing the sensitivity of metal ion sensors by using ion- selective peptides 1.2 Metal Ion Sensors The detection and quantification of metal ions in aqueous solutions is an important analytical ... removing metal ions is based on Chapter Introduction the adsorption of metal ions onto a sorbent, which is usually modified with some functional groups (ligands) to form complexes with the metal ions ... attracted considerable attention in a number of fields such as bioassays, biosensors, and metal ion sensing applications 1.1 Adsorption of Metal Ions The presence of heavy metal ions in the environment...

Ngày tải lên: 12/09/2015, 10:22

... ion- exclusion partition chromatography, Donnan exclusion chromatography, and ionmoderated partition chromatography It has been demonstrated that the retention of Applications of Ion Chromatography ... Donnan exclusion chromatography: I Theory and application to the separation of phosphorus oxoanions or metal cations J Chromatogr 1980;201:259–264 46 RETENTION PROCESSES IN ION- EXCLUSION CHROMATOGRAPHY: ... separation of anions and cations on weak cation-exchange stationary phases, and the use of indirect spectrophotometric detection in IEC REFERENCES Wheaton RM, Bauman WC Ion exclusion, a unit operation...

Ngày tải lên: 22/03/2014, 09:20

... Agent 158 Weak-Acid Ion Exchangers 159 Chelating Ion- Exchange Resins and Chelation Ion Chromatography 161 Fundamentals 161 Examples of Metal- Ion Separations 162 Ion- Exclusion Chromatography 8.1 ... Development of Ion- Exchange Separations 2.1 2.2 2.2.1 2.2.2 2.2.3 2.3 2.3.1 2.3.2 2.3.3 Introduction 23 Separation of Cations 26 Cation Separations Based On Affinity Differences 26 Cation Separations with ... Solvents 27 Separation of Anions 28 Separation of Anions with the Use of Affinity Differences 28 Anion Separations Involving Complex Formation 28 Effect of Organic Solvents 30 Ion- Exchange Resins...

Ngày tải lên: 04/06/2014, 15:25

... conversion reactions have intrinsically limited reversibilities and large volume changes of the conversion-reaction-based anode materials during lithiation/delithiation could lead to pulverization ... are[8]: (i) A transition metal ion in the insertion compound cathode with large work function to maximize the cell voltage, (ii) large amount of lithium insertion/extraction to maximize the cell ... intensively [8, 16] i Layered transition metal oxides ii Mn-based spinels iii Polyanion-type cathode materials 27 1.3.2.1 Layered transition metal oxides Layered transition metal oxides cathode materials...

Ngày tải lên: 10/09/2015, 09:27

... Schematic representation of the formation of IONP@mC-600 (A) IONPs and F127 micelles, (B) resol-Fe3O4 and resol-F127 monomicelles, (C) IONP@PC, (D) IONP@mC, (E) cross section of IONP@mC, and (F) ... TEM images of one IONP@PC and IONP@mC-600 Insets of C and F: SAED patterns of IONP@PC and IONP@mC-600 95 Figure 6-4: Particle size distributions of (A) IONP@PC and (B) IONP@mC-600 96 Figure ... of (A) IONP@PC and (C) IONP@mC-600 High resolution XPS spectra of C1s from (B) IONP@PC and (D) IONP@mC-600 103 Figure 6-11: (A) XPS and (B) high resolution XPS spectra of C1s from IONP@mC-450...

Ngày tải lên: 10/09/2015, 09:30

... or formation/ decomposition of Limetal alloy Chapter I describes the LIBs, principle of operation, development of LIBs, applications, world market and need for R&D and selection criterion of electrode ... Li -ion batteries I.3 Principle of operation of LIBs I.4 Applications and World market I.5 Need of R&D and selection criterion of electrode materials for LIBs I.6 Prospective second generation ... Lattice distortion due to Jahn- Teller effect because of large concentration of Mn3+ ion during high C-rate insertion of Li at the particle surface (ii) The dissolution of Mn2+ ion in the solvents...

Ngày tải lên: 11/09/2015, 10:17

... Reaction 1.9.3.1 Binary transition metal oxides 1.9.3.2 Ternary and complex transition metal oxides 1.9.4 Oxide Anodes based on Reversible Li -metal- oxide Bronze 33 33 37 38 Formation/Decomposition ... Transition metal oxides that react with Li via displacive redox reaction MO (M=Co, Fe, Ni, Cu) Transition metal oxides that react with Li via Li- metal oxide bronze formation (Mo, W) Following sections ... papers [3-6, 9-14] 1.3 Principle of Operation The operation of LIB is based on the principle of insertion/de-insertion of Liions in electrode materials via an ionically conducting medium called electrolyte...

Ngày tải lên: 16/09/2015, 08:32

... 19 : LUYÊN TẬP VỀ : LIÊN KếT ION LIÊN KẾT CỘNG HOÁ TRỊ SỰ LAI HOÁ CÁC OBITAN NGUYÊN TỬ Liên kết phân tử KF thuộc liên kết A cộng hoá trị B cộng hoá trị phân cực C ion D cho - nhận Hãy chọn phương ... HCl, CsF, H2O NH3 Hợp chất có liên hết ion A HCl B CsF C H2O D NH3 Chọn đáp án Các liên kết phân tử NH3 thuộc liên kết A cộng hoá trị B cộng hoá trị phân cực C ion D cho - nhận Hãy chọn đáp án Nguyên ... kết A ion B cộng hoá trị không phân cực C cộng hoá trị phân cực D cho - nhận Hãy chọn đáp án Liên kết phân tử HBr liên kết A cộng hoá trị không phân cực B cộng hoá trị phân cực C cho -nhận D ion...

Ngày tải lên: 14/08/2012, 10:01

... môi trờng Sheet Metal: Mở file mẫu Sheet Metal chọn mặt chi tiết file Part, sau chọn Application-> Sheet metal Hệ thống cho phép sử dụng công cụ Sheet Metal bổ sung tham số Sheet Metal tới file ... Sheet Metal Một chi tiết Sheet Metal có tham số mô tả chi tiết cách tạo Ví dụ Sheet Metal thờng có chiều dày mặt uốn cong thờng có bán kính Nút Sheet Metal Styles biểu tợng công cụ Sheet Metal ... đổi kiểu dạng Part Model mặc định thành Sheet Metal Kiểu Sheet Metal chi tiết chi tiết cho phép sử dụng công cụ Sheet Metal bổ sung tham số Sheet Metal Khi ta chuyển lại thành mô hình chi tiết...

Ngày tải lên: 27/08/2012, 13:54

Ngày tải lên: 12/09/2012, 22:32

... speciation of heavy metals Relationship of metal speciation and leachable metal Since a M MgC12 solution is a stronger extractant than a 0.01 M CaC12 solution for metals from cation sites, more metals ... variation of DTPA extracted metals seems to be related to the changes in metal speciation and will be discussed below Metal speciation To determine the effect of red mud on the speciation of metals ... Oxidesbound Organicfraction Residue oooo=oogo ~- Time (days) end red mud addition (%) Fig 11 The variation of Ni speciation in sludge composting with the red mud addition Metal speciation of digested...

Ngày tải lên: 23/09/2012, 14:47

... concentrations of the metal ion in solution (mg/litre), V is the solution volume (litres) and m is the mass of sorbent (g) The solid/water distribution ratios (at equilibrium) of metals for both sorption ... non-leachability of metals from the metal- loaded sorbents EXPEilIMENTAL Materials and methods All heavy metal solutions (divalent cations Of Pb, CA and Cu) were prepared in stock solutions up to 10000 ... occupation of active surface sites of sorbent with metal loading in the aqueous solutions (Apak et al., 1995, 1996) As long as a strict differentiation between true adsorption and precipitation-...

Ngày tải lên: 23/09/2012, 14:47