imaging—99mtc labeled red blood cells

... Glutaraldehyde has the ability to fix red blood cells to stabilize the membranes for use in agglutination studies Without glutaraldehyde stiffening process, the red cells were very easily deformed ... Scanning Procedures Blood was extracted from the author’s finger, anticoagulated with heparin and used immediately after extraction In order to visualize the blood cells with AFM, the cells have to ... and Notations ix Summary x Chapter Introduction 1.1 Red Blood Cell Membrane 1.2 Micro-rheology of Red Blood Cell Membrane 1.3 AFM and its Application on RBC Membrane Rheology...

Ngày tải lên: 30/09/2015, 14:23

... independent methods blood cell count RBCs (n=5) (cells/ µL) cytometer white blood cells platelets (cells/ µL) (cells/ µL) 6600000±2000 n.d.* n.d.* FACS 6300000±1520 n.d.* n.d.* blood slide 6400000±120 ... 2005;115:3370-3377 18 Kleinbongard P, Schulz R, Rassaf T et al Red blood cells express a functional endothelial nitric oxide synthase Blood 2006;107:2943-2951 19 Newman PJ, Gorski J, White GC2 et ... in human blood Mutat.Res 2002;515:1 Dertinger SD, Camphausen K, Macgregor JT et al Three-color labeling method for flow cytometric measurement of cytogenetic damage in rodent and human blood Environ.Mol.Mutagen...

Ngày tải lên: 03/11/2012, 10:58

... fi 3-linked sialic acids [19–21] Fig MALDI-TOF mass spectra of free, nonreduced N-glycans isolated from cRBCs Peaks appeared in the mass range 2000–3600, with the most prominent peaks at m ⁄ z ... MALDI-MS spectra of sialidase S-treated, unlabeled, N-glycans that were released from cRBCs by PNGase F (B) 1H-NMR spectra of sialic acid linkage in the free nonreduced N-glycans isolated from cRBCs ... the entire N-glycan pool, we labeled cRBC glycans with 2AB, separated them into oligosaccharide pools and quantified these pools using HPLC N-glycans from fetuin were labeled and used as a standard...

Ngày tải lên: 14/02/2014, 19:20

... lower (~60% vs >95%), compared to the 293 cells On Northern blot we were not able to detect the shRNAs transcribed from the H1 promoter in the QCE-6 cells while the 293 cells produced high amount ... effect of NO COS cells were killed by NO as tested by the addition of NO-donors Similar cytotoxicity was observed when COS cells were co-cultured with macrophages and this effect was reduced by NOS ... treatments recovered the redox homeostasis of obese rats GSPE acts as a free radical and ROS scavenger while OE, due to its slimming effects and mobilization of internal fat stores, seems to reduce the...

Ngày tải lên: 30/03/2014, 20:20

... nucleated red blood cells (NRBCs) in the blood In-hospital mortality of medical intensive care patients in relation to the concentration of nucleated red blood cells (NRBCs) in the blood Numbers ... measured on the day of the first appearance of nucleated red blood cells in blood n.s., not significant APACHE II, Acute Physiology and Chronic Health Evaluation II; NRBC, nucleated red blood ... Incidence of nucleated red blood cells in the blood of hospitalised patients Infusion Ther Transfus Med 2001, 28:263-266 Stachon A, Sondermann N, Imöhl M, Krieg M: Nucleated red blood cells indicate...

Ngày tải lên: 13/08/2014, 03:21

... Figure Predicted risk of death against maximum age of red blood cells A locally weighted nonparametric smoother (LOWESS) for the predicted probability of death and the maximum age of red blood cells ... post-transfusion viability to deformability of stored red cells Br J Haematol 1983, 53:237-240 Card RT, Mohandas N, Perkins HA, Shohet SB: Deformability of stored red blood cells Relationship to degree of packing ... Hospital mortality according to maximum age of red blood cells Hospital mortality (%, 95% confidence interval) according to the maximum age of red blood cells (RBCs) (days) Patients with the maximum...

Ngày tải lên: 14/08/2014, 08:21

... photobleaching GAG sulfated glycosaminoglycan HSP heat shock protein nRBCs normal red blood cells iRBCs infected red blood cells KAHRP knob associated histidine rich protein PAM pregnancy-associated ... asexual stage is considered to be important to parasite multiplication inside the human body, and the altered mechanical properties of infected red blood cells (iRBCs) are considered to be the main ... developed After merozoites mature, they enter into the blood circulation Merozoites can then invade red blood cells (RBCs), multiply inside the cells, and produce proteins modifying the cell rigidity...

Ngày tải lên: 09/09/2015, 18:52

... exoerythrocytic schizonts, the released merozoites flow into the blood stream to infect red blood cells Parasite invasion of red blood cells (RBCs) involves specific interactions between merozoite ... Plasmodium falciparum Erythrocyte Membrane Protein parasitized red blood cells phosphatidylserine parasitophorous vacuole membrane red blood cells ring-infected erythrocyte surface antigen Roswell Park ... the total blood volume Whereas P malariae only infects older red cells with the number even more limited The unique sequestration or cytoadherence property of P falciparum infected red cells may...

Ngày tải lên: 11/09/2015, 14:34

... Phosphatidylserine exposure of stored red blood cells 103 4.5.7 Membrane redox activity of young and old red blood cells 105 4.5.8 Surface structure of young and old red blood cells 105 Discussion ... ageing of red blood cells 23 2.5.1 Young and old red blood cells 23 2.5.2 Ca2+ content in young and old red blood cells 24 2.5.3 Influence of ageing on membrane redox system ... Ca2+ content in sickle red blood cells 57 4.1.5 Investigation of Ca2+ uptake in sheep red blood cells 60 4.2 Investigation of phosphatidylserine exposure in red blood cells 63 4.2.1 Phosphatidylserine...

Ngày tải lên: 15/11/2015, 12:54

... 12 10 1.5 B cells Normalized Expression Levels Normalized Expression Levels PBMC Monocytes T cells 14 0 C LXRβ 11x - + - + PBMC Monocytes - + T cells - + B cells Monocytes T cells B cells LXRβ ... peripheral blood ABCA1 and ABCG1 gene expression is directly regulated by LXR 60kDa LXRα α Figure LXRs are expressed in peripheral blood cells LXRs are expressed in peripheral blood cells (A) ... enriched in immune system cells, was compared to induction in peripheral blood cells For GW3965, there was a strong correlation between the induction of ABCA1 or ABCG1 in the blood and spleen (Figure...

Ngày tải lên: 18/06/2014, 15:20

... In the United States, more than 14 million units of packed red blood cells (RBCs) are administered annually, many of which are administered in the ICU [9] Approximately 40-80% of RBC transfusions ... with waiver of written informed consent (# 09-953) Red blood cell transfusion characteristics Packed red blood cell units were obtained from the blood bank (St John’s Mercy Medical Center) None ... GW, Rush BFJ: Decreased red blood cell deformability and impaired oxygen utilization during human sepsis Am Surg 1993, 59:65-68 37 Baskurt OK, Gelmont D, Meiselman HJ: Red blood cell deformability...

Ngày tải lên: 20/06/2014, 22:20

... meta-analyses of leukoreduced blood transfusions in surgical patients Transfusion 2007, 47:573-581 73 Fergusson D, Khanna MP, Tinmouth A, Hebert PC: Transfusion of leukoreduced red blood cells may decrease ... storage on clinical outcomes: The Red Cell Storage Duration Study (RECESS) (Section 7) Tranfus Apher Sci 2010, 43:107-116 Ness PM: Does transfusion of stored red blood cells cause clinically important ... red cell concentrates during storage is prevented by prestorage white cell reduction Transfusion 1997, 37:678-684 Anniss AM, Sparrow RL: Storage duration and white blood cell content of red blood...

Ngày tải lên: 20/06/2014, 23:20

... Blood Group Antigens 92 ABO Blood Group 92 Rh Blood Group 97 P Blood Group 98 Lutheran Blood Group 99 Kell Blood Group 99 Lewis Blood Group 100 Duffy Blood Group 101 Kidd Blood Group 102 LW Blood ... reported that the red cells are composed of water (721 e 17 mg mLÀ1 of the red cells) , proteins (371 mg mLÀ1 of the red cells) , most of which is hemoglobin (335 mg mLÀ1 of the red cells) , lipids ... to these results in human red cells, it is interesting to note that PC is totally absent in the red cells of sheep and is reduced markedly in camels, and in the red cells of rats it is significantly...

Ngày tải lên: 29/06/2014, 11:20

... 14C-thymidine; to reveal domains in cultured endothelial cells using the native isotopes 12C, 16O, 14N and 31P; and to track a few 15N -labeled donor spleen cells in the lymph nodes of the host mouse ... MIMS images of cochlear hair cells from mice after days on the 15N-L-leucine diet DC, Deiter cells; OP, outer pillar cells; RL, reticular lamina; TBC, tympanic border cells (below the basilar membrane); ... 12C15N/12C14N- ratio of a sample containing both Teredinibacter turnerae (Tt; rod-like cells) and Enterococcus faecalis (Ef; bunches of rounded cells) cultured in a 15N atmosphere for 120 h Field: 46...

Ngày tải lên: 06/08/2014, 18:21

Ngày tải lên: 07/08/2014, 18:21

... etateca lyhthpan-α :EANA ,B kcalb naduS :BBS ,esadixorep :REP )+++( gnorts ,)++( gnorts ot etaredom ,)+( etaredom ,)*+( gniniats ylkaew ,)-( evitagen† *+ *+ +++ steletalP )ralunarg( + + + setycohpmyL ... suoirav fo yrtsimehcotyc fo yrammuS † eht sa SAP dna ulg-β ,BBS ,REP rof evitisop ylgnorts ot yletaredom erew yehT ebol nitamorhc xes delaever selamef eht morf slihportuen fo segatnecrep eerht ot ... REP rof evitagen )C( W htiw msalpotyc ni selunarg elprup krad wef a )B( GW htiw msalpotyc deniats-redneval dna suelcun debol egral htiw lihposab a )A( :slihposaB SAP ,msalpotyc atnegam gnorts ni...

Ngày tải lên: 07/08/2014, 20:23

... RA synovium cells were considered to respond better to exogenous IL-18 than the blood cells did IL-18BP decreased IL-12-stimulated IFN-γ production from total RA synovium cells cultured with or ... than peripheral blood cells [12] In addition, Haddad and colleagues reported that activated whole blood cells from RA patients produced higher levels of IL-4 and lower IFN-γ than did cells from healthy ... activity Next, we compared the response to TH1-inducing cytokines of blood versus synovium cells from RA patients Judging from the effect on IFN-γ production, total RA synovium cells showed a greater...

Ngày tải lên: 09/08/2014, 01:23

... unable to predict individual responsiveness to TBA [14] Therefore, to enable such a prediction, global approaches based on proteomics or transcriptomics have been recently considered [27,28] ... series of transcripts whose altered levels could point to the physiopathological importance of a predominating function or pathway Indeed, these transcripts covered such diverse proteins and functions ... validation subsets (subset in text and Tables and 3) In any given sample of peripheral blood mononuclear cells, the abundances of informative transcripts were determined by qRT-PCR and normalized...

Ngày tải lên: 09/08/2014, 08:22

... peripheral circulating redblood cells CircadianClock mutant mice Circadian variations in peripheral circulating redblood cells (RBC) in Clock mutant mice (A) Number of RBC and (B) blood levels of hemoglobin ... circulating blood cells To determine peripheral circulating blood cells at each time point and genotype, mice were killed by decapitation, trunk blood was collected into heparinized capillary tubes, blood ... determine whether intact CLOCK protein is required for the circadian changes in peripheral blood cells, we examined circulating WBC and red blood cells (RBC) and evaluated plasma levels of corticosterone...

Ngày tải lên: 10/08/2014, 09:20

... (≤6 units) blood product exposure and reduced long-term survival [3] As the risk of transfusion-associated adverse outcomes may depend on the amount of transfusion [4], reduction of blood transfusions ... blood transfusions is considered a relevant, important goal in cardiac surgery During cardiac operations under CPB, two concurrent events, namely blood loss and red blood cell dilution due to positive ... Microulis D, Giannoukas A, Tsilimingas N: The impact of fluid restriction policy in reducing the use of red blood cells in cardiac surgery Acta Anaesth Belg Rogers MA, Blumberg N, Saint S, Langa...

Ngày tải lên: 10/08/2014, 10:20