ii—in vitro fertilisation and intracytoplasmic sperm injection

... C-terminal residues of each b-strand and the loops that connect each strand, bn, to the following helix, an) from those that stabilize the overall fold (the core b-barrel and the loops connecting an ... consistent with a proposed critical role for loop (which is mobile and thought to adopt different conformations in unliganded and ligand-bound states) in binding PRA More importantly for this study, ... of PRAI and trPRAI-His as monitored by CD at 219 nm Raw data and smoothed curves are shown (B) Fluorescence emission spectra of PRAI and trPRAI-His The excitation wavelength was 280 nm and the...

Ngày tải lên: 30/03/2014, 20:20

... Maniatis, T., Ruskin, B., and Green, M R (1984) Normal and mutant human /3-globin pre-mRNAs are faithfully and efficiently spliced in vitro Cell 36,993-1005 Krieg, P A and Melton, D A (1984) Formation ... mM dTTP, and mM mSdCTP 50X second-strand RX nucleotides: 7.5 mM dATP, 7.5 mM dGTP, 7.5 mA4 dTTP, and 35 mM dCTP 50X second-strand XR nucleotides: 7.5 n&f dATP, 7.5 mM dGTP, 7.5 mM dTTP, and 10 ... 10 mM spermidine, and 500 pg/mL BSA 20 10X kinase buffer: 500 mM Tris-HCl, pH 7.5, 100 mM MgC&, 50 mM D’IT, mM spermidine, and mM EDTA 21 10X annealing buffer: 200 mM Tris-HCI, pH 7.5, and 500...

Ngày tải lên: 11/04/2014, 09:47

... conducted by standard agar well diffusion method The E coli DH5a, M luteus and S aureus strains were grown on LB Broth at 37°C overnight upto a turbidity of 0.5 Mac Farland standard (108 CFU ... Couvreur, A Andremont, Antimicrob Agents Chemother 33, 1540 (1989) 19 E Fattal, J Rojas, L Roblot-Treupel, A Andremont, P Couvreur, J Microencapsul 8, 29 (1991) 20 H Pinto-Alphandary, O Balland, M ... ampicillin, streptomycin and kanamycin along with their respective Gnps conjugated forms against E coli DH5a, M luteus and S aureus in Luria-Bertani (LB) broth were determined by standard method [29]...

Ngày tải lên: 22/06/2014, 06:20

... conducted by standard agar well diffusion method The E coli DH5a, M luteus and S aureus strains were grown on LB Broth at 37°C overnight upto a turbidity of 0.5 Mac Farland standard (108 CFU ... Couvreur, A Andremont, Antimicrob Agents Chemother 33, 1540 (1989) 19 E Fattal, J Rojas, L Roblot-Treupel, A Andremont, P Couvreur, J Microencapsul 8, 29 (1991) 20 H Pinto-Alphandary, O Balland, M ... ampicillin, streptomycin and kanamycin along with their respective Gnps conjugated forms against E coli DH5a, M luteus and S aureus in Luria-Bertani (LB) broth were determined by standard method [29]...

Ngày tải lên: 22/06/2014, 19:20

... 0.6 0.6 8.3 1.1 15.0 70.8 3.5 The bands a at 2850–3000 cmÀ1 (nCH2 and nCH3) and b at 1745 cmÀ1 and 1702 cmÀ1 (nCO), related to vibrations of alkyl chain, ester and free carboxyl groups, respectively, ... of lentinan samples and the immunologically active b-glucan from Saccharomyces cerevisiae The arrows indicate absorption bands typical of lipids (a and b), and proteins (c and d) Normal MCF-7 ... peptone 0.3% and glucose 1.0% The inoculum, 500 ml, was grown in the same medium and the duration of fermentation was days The fruiting bodies and mycelia were dried by lyophilization and powdered...

Ngày tải lên: 28/06/2014, 11:20

... evitimirp dna dettimmoc ni hcir si sesutef namuh mreterp morf doolb droc lacilibmU A zciwopiliF -randoW ,A illehciT ,W evergzloH ,D kebruS ,C nessiN ,E aiaksvolkhC ,W nesnaJ ,V eranobraC ellad ,A...

Ngày tải lên: 07/08/2014, 18:21

... in vitro migration and invasion activity and induced the expression of HSPA4 and HSPA14 We previously demonstrated that NBS1 overexpression induces epithelial-mesenchymal transition in head and ... in vitro migration and invasion activity in a lung cancer cell line NBS1 expression vector was transfected into H1299 cells and stable clones were generated (Figure 1A) In vitro migration and ... Among the numerous candidates, we focused on two heat shock Figure NBS1 overexpression induced in vitro migration and invasion activity of H1299 cells and the expression of HSPA4 and HSPA14 (A) Western...

Ngày tải lên: 10/08/2014, 05:21

... date, and to the best of our knowledge, no plant promoter showing specific inducible activity during in vitro culture has been isolated and utilized Promoters with high and/ or specific in vitro ... gene in lines 156, 49 and 111 In addition, T-DNA tandem repeats were identified in lines 156 and 49, and vector backbone sequences were integrated in lines 17, 156, 49 and 179 (data not shown) ... promoters and genes in banana and to follow their expression pattern throughout in vitro development and LT treatment In addition, with the aid of combined PCR techniques we were able to identify and...

Ngày tải lên: 12/08/2014, 03:20

... mammary tumors [5,6], and immunohistochemical detection of Bcl2, p53 and cytokeratins, in human and canine tumors and corresponding adjacent tissues, have been similar [8] In dogs, standard treatment ... Suppressor 1), and CLPTM1 (Cleft Lip- and Palate-Associated Transmembrane Protein 1), which could correctly classify 95% of the samples, and another one, was PRSS11, MTSS1, and SMYD2 (Set and Mynd ... Veterinaria Scandinavica 2008, 50:27 http://www.actavetscand.com/content/50/1/27 Patients were evaluated by clinical history and physical examination including mammary tumor measurement and inguinal and...

Ngày tải lên: 12/08/2014, 18:22

... image analysis and compilation skills, Dr Karthikeyan Kandasamy for some of the qPCR experiments and Dr Rensheng Deng and Mohammed Shahrudin Ibrahim for providing the membranes and bioreactors ... and OAT3 and organic cation transporters (OCTs), primarily OCT1 and OCT2 (8, 23, 24) 13 Glucose reabsorption in the PTC occurs in two steps: 1) through Na+-glucose cotransporters and (SGLT1 and ... the quality and pre-treatment of the cells In addition, HPTC dedifferentiate and transdifferentiate under in vitro conditions and this depends, at least in part, on the actual in vitro culturing...

Ngày tải lên: 09/09/2015, 10:19

... particle synthesis, and metal loading RP conceived of the study, and participated in its design and coordination, carried out in vitro toxicity studies, SEM and EDX analysis and drafted the manuscript ... concentrations of A- and HA being 95% and 5% at pH and 15 and 85% at pH 4, respectively (pK a = 4.75) Furthermore, even though the concentration of the anion changes in this case as well, and is higher ... experiments illustrate both pH- and competing ligand-dependent effects on the rate of Cu release (see Discussion) In vitro toxicity of CuCNPs in HeLa cells The in vitro toxicity of CuCNPs in HeLa...

Ngày tải lên: 21/06/2014, 02:20

... phenotype and direct the cells to form a new and appropriate matrix Materials and methods Mesenchymal stem cell source and extraction Bone marrow samples were received from two patients (aged 66 and ... MSC sample 1; and two explants injected with MSC sample 2) were removed at 48 hours, week, weeks and weeks post injection Processing of tissue explants and identification of the injection site ... [39,40] Immunofluorescence and immunohistochemistry Immunofluorescence and immunohistochemistry were both performed for aggrecan, Sox-9, and types I, II and X collagen, and immunohistochemistry...

Ngày tải lên: 09/08/2014, 01:22

... design, and was responsible for data acquisition, analysis and interpretation as well as manuscript preparation CMUH, CVH, DMA, NM, HR, JDI and RH contributed to study design and data analysis and ... the 488 excitation laser and emission was detected between 500 and 560 nm Images were collected using 0.5 μm Z-steps and these were projected using maximal projection and overlaid with single ... treatment CII, stained and imaged at the same gain and offset levels as the positive cells and no fluorescence was observed Results Mechanisms of CII uptake in macrophages and DCs To study the...

Ngày tải lên: 09/08/2014, 08:22

... completed, and we await the results SP-B and SP-C are synthesized by ATII cells as proproteins (proSP-B and proSP-C) that are processed to mature SP-B and SP-C by serial cleavage of NH2- and COOH- ... included males and females with age ranges from 10 to 72, and there were current smokers and nonsmokers Hence, there was a significant amount of variability among the donors, as expected Ito and Mason ... 1.080 and 1.040, and by negative selection with CD14-coated magnetic beads (Dynal Biotech ASA, Oslo, Norway) and binding to IgGcoated Petri dishes (Sigma, St Louis, MO) The cells were counted and...

Ngày tải lên: 12/08/2014, 11:23

... MR carried out the in vitro and in vivo experiments, analysed and interpreted the data and drafted the manuscript DS participated in design and coordination of the study and revised the manuscript ... of the study and revised the manuscript LC conceived of the study and participated in its design and coordination, aided with statistics and revised the manuscript All authors read and approved ... Institute for Biological Standards and control (Potters Bar, UK) and coating antibody for IFN-γ by BD Biosciences (San Diego, CA, USA) The recombinant proteins for IL-1β, IL-6, IL10 and IL-13 were provided...

Ngày tải lên: 12/08/2014, 14:22

... http://arthritis-research.com/content/12/6/411 up ACS-based randomized controlled trials with full patient and observer blinding and with unconditioned serum as control in order to provide a ... autologous conditioned serum for treatment of osteoarthritis, in vitro effects on cartilage metabolism and intra-articular levels after injection Arthritis Research Ther 2010, 12:410 Rutgers M, Saris ... autologous conditioned serum for treatment of osteoarthritis, in vitro effects on cartilage metabolism and intra-articular levels after injection Arthritis Research Ther 2010, 12:R114 Darabos N, Hundric-Haspl...

Ngày tải lên: 12/08/2014, 15:22

... saline (PBS), and resuspended at × 106 cells /mL in Annexin-V binding buffer The supernatant (100 μL/tube) was incubated with μL of Annexin-V-FITC (Invitrogen, USA) and μL of PI (Invitrogen, USA) ... means ± standard deviations, and samples were subjected to multiple analysis of variance The Dunnett-t test was performed to compare the mean between each test group and control group, and the ... for combretastatin A4: design, characterization, and in vitro evaluation AAPS Pharm Sci Tech 2006; 7:E32 Qiu L, Jing N, Jin Y Preparation and in vitro evaluation of liposomal chloroquine diphosphate...

Ngày tải lên: 26/10/2012, 08:57

... 2010, pp.221-248 231 Table Start of injection timings and injection duration for hydrogen operation in port and manifold injection S.No Start of Injection Injection Duration Crank angle (Degrees) ... Optimized injection data for port and manifold injection Injection Parameter Start of injection of hydrogen Hydrogen injection duration Hydrogen flow rate Exhaust gas Recalculation Port Injection ... manifold injection Table EGR flow percentages used in port and manifold injection Port injection S.No Manifold injection Optimized timings and duration EGR flow percentage, (%) Optimized timing and...

Ngày tải lên: 05/09/2013, 15:28

... strand Both the strands can be used as templates for DNA sequencing reactions The immobilized strand can be stored and reused for further reactions into solution and leaving the other strand ... DNA from a primer annealed to a single-strand template DNA molecule In general for PCR products the template will be doublestranded and is heat denatured and rapidly frozen by placing in dry ice ... containing nonpolymerized gel matrices and laser detection systems The introduction and removal of polymer from the capillaries, plus loading and running samples and fragment detection, are automated...

Ngày tải lên: 25/10/2013, 22:20

... pathways with in vitro cytosol and cell-free mitochondria, our data suggest a direct interaction between ETA and cytosolic EF-2 on the one hand, and the mitochondrial membrane on the other hand However, ... liver in vivo model versus cellular in vitro systems) and ⁄ or may be related to differences between hepatocytes and other cell types In vivo [36] and in vitro [37] studies have shown that the ... We report on the in vivo association of ETA and ETA-A with cytosolic fractions, and the in vitro ATP-dependent and pH-dependent translocation of ETA and ETA-A from cell-free endosomes into the...

Ngày tải lên: 18/02/2014, 04:20