... Ames, B.N (19 98) DNA oxidation matters: the HPLC-electrochemical detection assay of 8- oxodeoxyguanosine and 8- oxo-guanine, Proc Natl Acad Sci USA, 6, 288 -293 Maron, D.M and Ames, B.N (1 983 ) Revised ... on the development ofthe total evaluation technique forthe hazardous impacts by the chemical substances towards human and ecology (the project leader is Prof Hideo Utsumi, Graduate School of ... C8-modified deoxyinosine and N2- and C8-modified deoxyguanosine as major products ofthe in vivo reaction of N-hydroxy-6-aminochrysene with DNA and the formation ofthe adducts in isolated rat hepatocytes...
... respectively One ofthe weaknesses of this study is the small sample size In addition, the majority ofthe lesions examined appear to be ofthe Class I type, rather than Class II lesions for which the technology ... positively with toluidine blue and of these were carcinomas One ofthe clinically detected carcinomas was dye-negative Therefore, out of 82 patients screened with the aid of toluidine blue and biopsied, ... the role of fluorescence visualization forthedetectionof surgical tumor margins for oral cancer when used in the operating room .86 Twenty consecutive patients undergoing surgical excision for...
... drops ofthe creatine solution, three drops ofthe ethanolic solution of 1-naphthol and then two drops ofthe potassium hydroxide solution; shake after the addition of each reagent The formation of ... the results ofthe interpretation, indicate the presence or absence of Salmonella in a test portion of x g or x ml of product (see ISO 72 18) See annex C forthe precision data obtained from the ... incubation for 24 h ± h, examine the plates (9.4.3) forthe presence of typical colonies of Salmonella and atypical colonies that may be Salmonella (see Note) Mark their position on the bottom of the...
... concentration of NO and NO2 gases because ofthe low concentration, one ofthe roles ofthe Ag is apparent to provide surface forthe conversion of NO to NO2 according to the literature [ 18] It is ... and was then diffracted onto the detector The detector collected data for all angles in the range 4±6 48, 2y, simultaneously Fig gives the diffraction spectra ofthe WO3 before and after the NO ... one ofthe roles of promoter may be to provide a surface forthe catalytic conversion of NO to NO2 that are responsible forthe high sensitivity associated with the promotion However, whether...
... between the 83 3 bp ofthe ORF59/60 junctional region between the "RDEL" and "PQFV" motifs of MneRV2 and the corresponding region of RRV, with an 87 % nucleotide identity The 83 4 bp sequence ofthe ... module for 45 cycles of 95°C for 30 s, 62°C for 30 s and 72°C for 30 s The copy number for each assay was calculated from the cycle threshold (CT) determined using the Bio-Rad software The viral ... rhadinovirus in the baboon, Papio cynocephalus, using the RV2 QPCR assay To further determine the specificity ofthe RV2 QPCR assay, DNA obtained from lymphocytes of baboon Pcy 784 04 was tested forthe presence...
... between the 83 3 bp ofthe ORF59/60 junctional region between the "RDEL" and "PQFV" motifs of MneRV2 and the corresponding region of RRV, with an 87 % nucleotide identity The 83 4 bp sequence ofthe ... module for 45 cycles of 95°C for 30 s, 62°C for 30 s and 72°C for 30 s The copy number for each assay was calculated from the cycle threshold (CT) determined using the Bio-Rad software The viral ... rhadinovirus in the baboon, Papio cynocephalus, using the RV2 QPCR assay To further determine the specificity ofthe RV2 QPCR assay, DNA obtained from lymphocytes of baboon Pcy 784 04 was tested forthe presence...
... differentiated hMSCs, also in the corner areas ofthe microcavity (Figure 3f) The performed studies verify the biocompatibility ofthe Si3N4 membrane and the suitability ofthe microcavity for in vitro studies ... Quantification of pIL8-GFP induction For quantification of an induced inflammatory reaction, the reporter cells pIL8-GFP A549 were used The induction ofthe promoter is linked to the production ofthe inflammatory ... and sensitivity, the use ofthe miniaturized system forthedetectionof threshold-dependent effects was tested The comparison ofthe data obtained using a 96-well microplate and the developed micro-sized...
... (12) then corresponds to the correlation ofthe real part, z1c(nT), ofthe SMF’s output, y1c(nT), with the known real symbols, an, over the known signal duration B Performance The performance of ... detectors as the phase diversity between the sources increases We still note the best performance ofthe completely informed detectors (C1 and O1) and the lowest performance ofthe less informed detectors ... statistical theory of detection, the optimal receiver forthedetectionofthe K symbols an from both the K primary data xv(nT) and the K’ secondary data bTv(nT)’ consists in comparing to a threshold the...
... Page of adjust the pH ofthe mixture to 9.1 under stirring The NaHTe solution prepared in the first step was added to the reaction mixture under N protection The resultant mixture was stirred for ... cutoff value in succedent detection was determined as a positive result The ten HPV-16-positive samples were used to validate our hybridization assay on the basis ofthe cutoff value Detectionof ... Page of predenatured at 95°C for 10 min, then 55°C for 30 The particles coupled with streptavidin were added into the hybridization mixtures and incubated at 37°C for 10 and enriched in the bottom...
... 0.52 28 and PD = 0 .88 53, while the accuracy increases to 68. 8% It is seen that the reject option reduces the probability of false alarm by approximately 7.3% at the same probability ofdetectionThe ... probability ofdetection approximately 73% for probability of false alarm 4% [33] Forthe same probability of false alarm, a probability of detection, which falls between 80 .95% for rectangular ... probability ofdetection close to 85 % could be achieved for a probability of false alarm 10% in the case of vocal fold paralysis in male utterances, while the probability ofdetectionfor vocal...
... selectivity when they are used just for Zn(II) detection On the other hand, the fluorescence enhancement of MWNTs-GNQ is only for Zn(II) As the use of organic–inorganic hybrid materials for bio-application ... intracellular environment forthedetectionof Zn(II) References ð1Þ Wherein, A is the value ofthe fluorescence intensity, C is the concentration of Zn(II), and the range of C is from 0.2 lM to ... MWNTs-GNQ Thus Zn(II) considerably enhances the fluorescence of MWNTs-GNQ To further characterize the performance ofthe sensing material for Zn(II), a series of comparison experiments were carried...
... of a widely used real-time industrial application The core component ofthe algorithm is the selection and the extraction ofthe features best describing the image contents The first step ofthe ... that the requirements ofthe automatic classification system of metallic surfaces are fulfilled We further emphasize the robustness and the simplicity ofthe proposed solution as no part ofthe ... illuminations must be used for covering the complete circumference of a metallic cylinder The number NC of needed cameras depends not only on the diameter ofthe object DO , the width ofthe ray aperture’s...
... normal PCR reaction of 50 µl volume (25µl of master mix, Comparison of Diagnostic Methods fortheDetectionof Parasites in Fish 2µl of each primer, or µl of template and 18 or 20 µl of water) - Polymerase ... Comparison of Diagnostic Methods fortheDetectionof Parasites in Fish and disadvantages, depending on the purpose and target of study For parasitological investigation, the classical methods (the ... impact in many areas of parasitology, including the identification and classification of parasites, the diagnosis of infections, the epidemiology of parasites, the analysis of population genetic...
... recommended for diagnosis of CHV in formalin-fixed tissues The optimized ISH assay, which developed in this study, may be a valid tool forthe study of pathogenesis and diagnosis of CHV infection ... Virus Infections of Carnivores pp 515, Elsevier, Amsterdam, 1 987 Kim O Development of in situ nest PCR and comparison of five molecular biological diagnostic methods forthedetectionof intracellular ... Field HJ A serological study of canine herpes virus-1 infection in the English dog population Arch Virol 19 98, 143, 1477-1 488 Reading MJ, Field HJ Detectionof high levels of canine herpes virus-1...
... RT-PCR forthedetectionof Japanese encephalitis virus 349 Fig Sensitivity of real-time RT-PCR assay for quantitative detectionof JEV (A) and detectionof JEV by RT-PCR (B) One milliliter of JEV ... assess the correct specificity ofthe real-time RT-PCR assay Both Anyang 300 and 347 Fig Comparison of real-time RT-PCR and gel electrophoresis analysis ofthe product forthedetectionof JEV ... Australia) The reaction mixture for each one tube TaqMan reaction mix consisted of µl of Universal 5X buffer, µl of 10 mM dNTP, µl of enzyme mix (Qiagen, Germany), µl of 20 pM forward primer, µl of 20...
... monitor the development ofthe antibody level using the yolk diluted 1,000 times with PBST The yolk antibody also showed no cross reaction to any other bacteria (Table 1), and the antibody levels of ... antigenic heterogeneity in the flagellins of Listeria monocytogenes and L ivanovii J Gen Microbiol 1 988 , 134, 2593-25 98 18 Skjerve E, Røvik LM, Olsvik Ø Detectionof Listeria monocytogens in ... Development of a sandwich ELISA forthedetectionof Listeria spp using specific flagella antibodies 43 washing, 100 µl of each HRP conjugated MAb was added into each well and incubation for 30 at...