identification quantitation and size analysis of viroplasms

... Laboratory of Crop Genetics and Germplasm Enhancement, Key Laboratory of Plant Nutrition and Fertilization in Low-Middle Reaches of the Yangtze River, Ministry of Agriculture, College of Resources and ... OsLPR3 and OsLPR4 and 67.2 % between OsLPR2 and OsLPR3 Amino acid SI was 68.3 % between OsLPR3 and OsLPR5 and 40.9 % between OsLPR2 and OsLPR5 The analysis suggested a relative closeness of OsLPR5 ... (UTR) of OsLPR4/5 were disrupted by an intron The analysis thus revealed both the divergence and conservation of LPR genes in Arabidopsis and rice Phylogenetic analysis of LPR genes LPR1 and LPR2...

Ngày tải lên: 22/05/2020, 04:34

... Structural and Functional Characterization of TTMP 4.2.1 Expression of TTMP in Normal Pancreas and Cancer Cell Lines 4.2.2 Identification of Translation Start Site and Molecular Size of TTMP ... Expression profile of TTMP in different normal tissues and cancer cells 66 12 Genomic organization and open reading frame of TTMP, and TTMP expression constructs 69 13 Molecular size of TTMP ... Deletion analysis of the 5’ flanking region of the hTTMP gene 86 25 Mutational analysis of the proximal promoter region of the hTTMP gene 87 26 Electrophoretic mobility shift analysis of nuclear...

Ngày tải lên: 14/09/2015, 22:09

... comparison and exon-intron organization In addition, the expression profile analysis of SlGRAS genes by real time qPCR in different stages of vegetative and reproductive development were performed, and ... SlGRAS38, and SlGRAS39) and three (SlGRAS15, SlGRAS37, and SlGRAS43) proteins belong to AtSHR and AtSCR subfamily, respectively Considering the important role of AtSHR and AtSCR proteins in root and ... of auxin and gibberellins in tomato fruit set J Exp Bot 2009;60:1523–32 Page 17 of 18 45 Kumar M, Khurana A, Sharma AK Role of plant hormones and their interplay in development and ripening of...

Ngày tải lên: 26/05/2020, 21:58

... aestivum) and rice (Oryza sativa): identification and bioinformatic analysis Mol Genet Genomics 2006, 276:478–494 Ma HL, Zhao J: Genome-wide identification, classification, and expression analysis of ... PCR analysis of representative OsXYLP genes in different developmental stages of vegetative and reproductive tissues and organs The expression levels of OsXYLP genes in different tissues and ... tandem and segmental duplications, such as tandemly duplicated gene pairs: OsXYLP11 and OsXYLP13, OsXYLP12 and OsXYLP14; and segmentally duplicated gene pairs: OsXYLP6 and OsXYLP8, OsXYLP9 and OsXYLP17...

Ngày tải lên: 27/05/2020, 00:20

... increased expression levels of AP1, FT, AG, and SEP3 might promote the formation of flowers and early flowering in transgenic plants (Fig 8a, b) The analysis of gene expression and phenotypic changes ... changes of GRF2 and TCP18 (Fig 8a), which was consistent with results that TCP [56] and GRF [57] could regulate leaf development In addition, the downregulation of ARF2 and TCP3 and up-regulation of ... development and ovules Zhang et al BMC Plant Biology (2020) 20:439 formation Our results expand the understanding of the development of stamens, pistils and ovules in Asteraceae family, and provide...

Ngày tải lên: 28/06/2021, 20:55

... Interesting New Gene5,6 and subsequently Key Laboratory of Genetics, Breeding and Comprehensive Utilization of Crops, Ministry of Education, College of Crop Science, Fujian Agriculture and Forestry University, ... dataset, of which RING-H2 (Bra030136 and Bra034259) and RING-HCb (Bra001543, Bra005642, Bra011996 and Bra027352) showed a value of zero for all the tested tissues and were excluded from the analysis ... percentages of genes per the total genes of each RING type in each expression group (Table 5) We can observe that 34.8% of RING-H2, 28.2% of RING-HCa, 52.2% of RING-HCb, 44.7% of RING-v, 34.4% of RING-C2...

Ngày tải lên: 04/12/2022, 10:38

... http://www.biomedcentral.com/1471-2229/11/127 Page 4 of 11 miRNA (vun_cand001, vun_cand010, vun_cand041, and vun_cand057) were found to be associated w ith drought stress for the first time (northern blot confirmation of vun_cand001 was ... of the world, the investigation of natural mechani sms of drought tolerance is an important strategy for under- standing the biological basis of response to drought stress and for selection of ... greater than 1 and the size of each asymmetrical bulge is 2 or less; (4) strand bias - small RNA reads that map to the positive strand of the hairpin DNA segment account for at least 80% of all mapped...

Ngày tải lên: 11/08/2014, 11:21

... sequences of the domains were aligned using ClustalX (v1.83) with a gap opening penalty of 35 and gap extension penalty of 0.75 in pairwise alignment, and a gap opening penalty of 15 and gap extension ... Because of the economic importance of this crop, and its evolutionary relationship to Arabidopsis thaliana, we sought to characterize a subset of canola WRKY genes in the context of pathogen and ... treatment [42] On the other hand, of JAresponsive TF in Arabidopsis, AtWRKY TFs are one of the greatest numbers of induced [45] Moreover, it is observed that cross-talk of SA- and JA-dependent defense...

Ngày tải lên: 12/08/2014, 03:20

... convergent and diver- gent pathways between salinity and other abiotic stress responses and the nodes of signalling convergence may greatly enhance the understanding of the salinity stress response and ... for agriculture. Decades of research on the effect of salinity on growth and development of various plants and their response to salin- ity treatment at the physiological and biochemical levels ... salt-induced expression of a large number of genes involved in a wide range of cellular functions was indicative of highly complex nature of the process as such. Most of the salt inducible genes,...

Ngày tải lên: 12/08/2014, 03:20

... kinesin-1 and kinesin light chain 13 Fig 4: Analysis of the purification fractions and the concentrate of hNCAM180ID 41 Fig 5: Analysis of the purification fractions and the concentrate of hNCAM140ID ... Co-IP of KLC1 and NCAM from mouse brain lysate 47 Fig 7: Immunofluorescence analysis of a CHO cell overexpressing NCAM and GFP-KLC1/KHC1 48 Fig 8: Immunofluorescence analysis of ... delivery of NCAM and NCAM∆CT to the cell surface in primary cortical neurons 60 Fig 15: Immunofluorescence analysis of cortical neurons overexpressing NCAM and KLC1 and detection of internalized and...

Ngày tải lên: 25/11/2015, 13:27

... duplication status, and expression profiling were carried out In addition, the evolutionary patterns of the LRR-RLK gene family were examined in soybean by analysis of genes in tandem and segmental ... genome The GmLRR-RLKs are non-randomly distributed across all 20 chromosomes of soybean and about 73.3 % of them are located in segmental duplicated regions The analysis of synonymous substitutions ... characterization of the LRR-RLK gene family in soybean Results and discussions Identification and genome distribution of LRR-RLK gene family in soybean In order to identify all members of LRR-RLKs...

Ngày tải lên: 22/05/2020, 04:05

... (https://www.arabidopsis.org) and Cucurbit Genomics Database (http://www.icugi.org) The map was obtained using Circos software ancestor of Arabidopsis and Citrullus after the divergence of monocots and dicots Analysis of ... (Zea mays), CYCLOIDEA (CYC) of snapdragon (Antirrhinum majus) and the PROLIFERATING CELL FACTORS and (PCF1 and PCF2) of rice (Oryza sativa) [16–18] Thus the name of the TCP TF family is derived ... stage, and in the flower and fruit, whereas ClTCP1a, ClTCP1b and ClTCP12a were only expressed in the internode and SAM beyond Shi et al BMC Plant Biology (2016) 16:85 Page of 13 Fig Phylogenetic analysis...

Ngày tải lên: 22/05/2020, 04:13

... chlorosis, browning of leaf points, decaying growing points, and wilting and dying-off of older leaves Brown margin of leaves, chlorosis, reddish veins and petioles, curled leaves, and brown stunted ... Engineering Handbook Section 16, Drainage of Agricultural Lands, and Section 18, Groundwater; Engineering Field Handbook Chapter 12, Springs and Wells, and Chapter 14, Drainage 5.4.1.2.1  Zones of Underground ... Estimates of future high, medium, and low groundwater levels are needed for engineering and architectural design decisions and for appropriate selection of land uses The elevation and shape of the...

Ngày tải lên: 12/08/2014, 02:20

... preventing failure of rock bridges and loss of interlock, and (2) to control and minimize rock mass bulking 4.2.1 Retention of self supporting capacity of rock mass The first function of reinforcement ... strength formulas of Hedley and Grant (1972) and Lunder and Palkanis (1997) as well as the database of Lunder and Palkanis (1997) are shown in Figure 3.20 (Note: beyond a pillar W/H-ratio of 2.5 there ... Because of the sensitivity of hard rock to stress change, a good understanding of the failure processes and stress path helps to assess the potential for caving of stopes, for dilution, and to...

Ngày tải lên: 11/07/2014, 15:20

... repressed by official knowledge and are “disqualified”, marginalized and silenced from the boundaries of official knowledge production Similarly, the neglect of ghostly bodies within studies of social ... the main thrust of this thesis is to unravel why and how the Pontianak has come to be imagined as “Malay” and the implications of these forms of imaginations on the urban landscape of Singapore ... emerge in the aftermath of times of swift and often traumatic change, when old social bonds have been unhinged and new group identities must be formulated The presence of ghostly haunting in many...

Ngày tải lên: 16/10/2015, 15:35

... the sentiments and views of men and of nations, forms a concert of wills, and a concurrence of action too powerful for the armies of tyrants’ (2 (1788): 324–5) As Thomas Holcroft more succinctly ... action and conservative propaganda, and with the break up of the radical associations and the consequent erosion of the links between these organisations and other intellectual currents and circles, ... more free, or more glorious: it is spread on the face of the earth, and is composed of persons of every nation, of every rank, of every age, and of both sexes They are intimately acquainted with...

Ngày tải lên: 21/09/2012, 11:00

... Levels of evidence With a somewhat ready assumption of cause and effect and, equally, a neglect of the laws of chance, the literature becomes filled with conflicting cries and claims, assertions and ... assumption of cause and effect, and, equally, a neglect of the laws of chance, the literature becomes filled with conflicting cries and claims, assertions and counterassertions It is thus, for want of ... revolutionary idea, of randomization, and this is so because without randomization, most of the rest of medicine’s discoveries would not have been discovered: it is the power of randomization that...

Ngày tải lên: 24/09/2012, 09:06

... and rural areas Ministry of Agriculture, Forestry and Fisheries Tokyo (published every year) Ministry of Environment of Japan (2008) Annual report on the environment Ministry of Environment of ... not available for the number of hens, these data are not listed in this table The population of H1 and H2 watersheds decreased by 10.3% and - 294 - Journal of Water and Environment Technology, ... respectively, between 1990 and 2005 In this period, the number of factories and animals also decreased In 2000, the population densities of H1 and H2 watersheds were calculated to be 46 and 78 inhabitants...

Ngày tải lên: 05/09/2013, 10:15

... activity of individuals or organizations of manufacturing and services, using modes and means of communications to inform the publics about their demands and ability or the benefits of given goods and ... countries and Vietnam Due to a shortage of time and within the limitation of an M.A thesis, the study only focuses on the analysis and comparison of 10 travel advertisements in each language Design of ... no ambition to make an analysis of language of adverting from all aspects such as phonological, lexical and morphological, syntactic and semantic aspect Within the scope of my study in this thesis,...

Ngày tải lên: 07/09/2013, 14:03

... GW and GK software, hardware, and processing capacity of the DSP modules of the GWs; and Developing a script or suite for determining the number of stages for setting up a prespecified number of ... also shows where and how (i.e., using what types of links) various kinds of test equipment are connected to support di¤erent types of tests and measurements A detailed description of the testbed ... determined size of the call bursts Although it is possible to implement a nonuniform distribution of bursts, for the sake of simplicity we use uniform call bursts here Let us set the size of the...

Ngày tải lên: 30/09/2013, 07:20