identification of potential protein binding sites in 4e
Báo cáo khoa học: "Histochemical Characterization of the Lectin-binding Sites in the Equine Vomeronasal Organ" potx
Ngày tải lên :
07/08/2014, 17:22
... examine the binding of DBA, SBA, Isolectin B4, WGA, PNA, and UEA-I in the vomeronasal organ of the horse We found unique characteristics of lectin binding depending cell types The microvilli of ... Characterization of the Lectin -binding Sites in the Equine Vomeronasal Organ 17 Fig Histochemical staining of DBA (A and B), SBA (C and D), and Isolectin B4 (E and F) in the vomeronasal sensory epithelium of ... Microvilli Receptor cell Supporting cell Basal cell Glands DBA ＋ ＋＋ － － － SBA ＋ － － － － －, No binding; +, infrequent (66% binding Lectin Isolectin B4 WGA ＋ ＋＋ － ＋＋ － － － － －...
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Báo cáo sinh học: "PhyloScan: identification of transcription factor binding sites using cross-species evidence" ppsx
Ngày tải lên :
12/08/2014, 17:20
... factor binding sites) fall into two general categories One approach is to employ a training set of transcription factor binding sites and a scoring scheme to evaluate predictions [2-8] The scoring ... Crp Binding Site Motif and Generation of Weaker Versions Crp Binding Site Motif and Generation of Weaker Versions The logo in panel A indicates the Crp motif used to scan for Crp binding sites ... statistically incorporate any sites other than the best found binding site in each intergenic region In another three pairs of runs we ran PhyloScan, permitting it to consider multiple sites within an intergenic...
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Báo cáo y học: "Genomic mapping of Suppressor of Hairy-wing binding sites in Drosophila" ppsx
Ngày tải lên :
14/08/2014, 08:20
... investigation of protein- DNA interactions in Drosophila Results Identification of Su(Hw) in vivo binding locations We have used ChIP-array to investigate the in vivo binding of the Su(Hw) protein in a ... the in vivo Su(Hw) binding sites It is interesting to compare our set of endogenous Su(Hw) sites with the gypsy insulator The 340 bp gypsy insulator contains a cluster of 12 Su(Hw) binding sites ... identified Su(Hw) binding sites Analysis of these endogenous Su(Hw) binding sites allowed considerable expansion of the Su(Hw) consensus binding sequence The existing Su(Hw) binding consensus was...
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Proteomics based identification of potential protein biomarkers for epithelial ovarian cancer
Ngày tải lên :
12/09/2015, 08:19
... 2003) These two proteins are members of the S100 Calcium -binding protein family They function as inhibitors of casein kinases I and II that are essential for phosphorylation of various molecules ... TOFTOF/MS 107 3.4 Validation of haptoglobin-α2 subunit using PS20 immunocapture proteinChip analysis 112 3.5 Validation of haptoglobin-α2 subunit as potential protein ... Confirmation of identification of ceruloplasmin and haptoglobin using 2-DE followed by Western blotting analysis 138 Figure 4.13 Western blotting analysis of ceruloplasmin and haptoglobin in cyst...
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Tài liệu Báo cáo Y học: Prediction of protein–protein interaction sites in heterocomplexes with neural networks ppt
Ngày tải lên :
21/02/2014, 15:20
... system is involved in many protein folding and traffic processes in the cell The main component of the system is DnaK, a two-domain protein with a C-terminal domain responsible for the binding of unfolded ... Predicting protein protein interaction sites (Eur J Biochem 269) 1357 More recently, methods were devised for predicting residues involved in protein interaction sites in the absence of any structural ... (http://trantor.bioc.columbia.edu/cgi-bin/SPIN/), which contains all the protein complexes contained in the PDB Protein Data Bank Using the SPIN search engine, it is possible to search the set of protein complexes for...
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Báo cáo khoa học: A hybrid clustering of protein binding sites ppt
Ngày tải lên :
15/03/2014, 10:20
... acid Basically, binding sites are represented by storing all the binding amino acid sequences of all the protein chains that are present at the particular binding site Binding sites were extracted ... determination fairly complicated An example of a binding site with four neighboring polypeptide chains can be seen in Fig Binding amino acid sequences were first extracted from the binding sites of ... protein binding sites The costs of aligned binding and nonbinding amino acids were as follows: l The cost of two aligned, different amino acids is unity l The cost of aligned, matching amino acids...
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Báo cáo khoa học: Carbohydrate binding sites in Candida albicans exo-b-1,3-glucanase and the role of the Phe-Phe ‘clamp’ at the active site entrance ppt
Ngày tải lên :
15/03/2014, 23:20
... diffusing too quickly away from the cell wall Surfacebased carbohydrate binding sites of weak affinity are well known (e.g in lectins and haemagglutinin) where Carbohydrate binding sites in Candida ... Carbohydrate binding sites in Candida exoglucanase Fig Key properties of the +1 subsite of Exg (A) Subsite binding energies based on published kinetic data for Exg-catalysed hydrolysis of b-1,3-linked ... structure of Exg involving an oligosaccharide and provides an insight into the enzyme’s mechanism of action in the C albicans cell wall Finally, the unexpected discovery of an isolated binding site...
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Support to the identification of potential risks for the environment and human health arising from hydrocarbons operations involving hydraulic fracturing in Europe doc
Ngày tải lên :
23/03/2014, 00:20
... e.g a discharge or incident resulting in potential effects on natural ecosystems in the vicinity of the release point or incident; ongoing effects on people in the vicinity of a site due to impacts ... pollution incident resulting in harm to the health of members of the public over a wide area due to contamination of drinking water supplies; accident resulting in death or serious injury to ... the identification of potential risks for the environment and human health arising from hydrocarbons operations involving hydraulic fracturing in Europe Overview of hydraulic fracturing in Europe...
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Báo cáo khoa học: Polysaccharide binding sites in hyaluronate lyase – crystal structures of native phage–encoded hyaluronate lyase and its complexes with ascorbic acid and lactose docx
Ngày tải lên :
23/03/2014, 04:21
... are involved in the interactions with protein ⁄ water atoms This is one of the most firmly held ascorbic acid molecules, indicating the strong nature of the binding of ascorbic acid to proteins ... subsites in the polysaccharide binding site of the TSbH domain of HylP2 The positions of ligands are indicated at the substrate binding groove (B) The view from the top shows the subsites of ascorbic ... attractive binding subsites in the protein, leading to the inhibition of the enzyme action Similarly, three molecules of lactose also bind in an almost identical manner and with subsites similar...
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Báo cáo khoa học: Co-operation of domain-binding and calcium-binding sites in the activation of gelsolin pptx
Ngày tải lên :
31/03/2014, 01:20
... C (2001) Binding of gelsolin domain to actin An actin interface distinct from that of gelsolin domain and from ADF/cofilin Eur J Biochem 268, 6165–6175 18 Lin, K.-M., Mejillano, M & Yin, H.L (2000) ... domain Footprint of G2 on gelsolin G4–6 domain Two sites within the G2 domain appear involved in the G2- G4–6 interface in the presence of EGTA (Fig 3) The interaction of these two sites (residues ... Identification of a region of segment of gelsolin critical for actin binding EMBO J 9, 4103–4109 24 Way, M., Pope, B & Weeds, A.G (1992) Evidence for functional homology in the F-actin binding domains of...
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Báo cáo khoa hoc:" Despite WT1 binding sites in the promoter region of human and mouse nucleoporin glycoprotein 210, WT1 does not influence expression of GP210" pptx
Ngày tải lên :
11/08/2014, 08:20
... regulation Strikingly, we could identify five putative Wilms tumor (WT1) suppressor protein binding sites, four Sp1 biding sites, and one ETS binding site in a range of 315 bases just upstream of the ... Wilms' tumor zinc finger protein 1; PuF, c-myc purine -binding transcription factor; IL-6 RE-BP, IL-6 Response element -Binding protein; NF-1, Nuclear factor 1; Ets-2, proto-oncoprotein; USF2, upstream ... dependent protein kinase sites (residues 1089, 1874), two tyrosine kinase sites (residues 227, 922), 30 protein kinase C phosphorylation sites and 26 casein kinase II phosphorylation sites were...
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Báo cáo y học: " Genome-wide investigation of in vivo EGR-1 binding sites in monocytic differentiation" doc
Ngày tải lên :
14/08/2014, 21:20
... binding 3.08E-019 GO:0003723 RNA binding 6. 44E- 018 GO:0005515 Protein binding 5.65E-009 GO:0000166 Nucleotide binding 3. 84E- 007 GO:0003677 DNA binding 5.69E-007 GO:0051082 Unfolded protein binding ... changed near EGR-1 binding sites, suggesting that EGR-1 binding influences the occupancy of previous binding proteins These observations may help explain why EGR-1 binding results in multiple responses ... SP1 binding occupancies change dramatically EGR-1 binding may influence the occupancy of previous binding proteins, resulting in the reconstruction of the transcription factor complex and the induction...
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Abundance and distribution of sharks in northeast florida waters and identification of potential nursery habitat
Ngày tải lên :
04/09/2015, 17:14
... the identification and delineation of suitable nursery habitat This has resulted in numerous ongoing and detailed studies examining the presence of shark nurseries in most of the major estuaries ... METHODS Sampling.—Longline sampling was conducted in the nearshore and estuarine waters of Cumberland and Nassau sounds (Figure 1) from late April through November using bottom longline fishing Weekly ... 2011:509–513), resulting in increased shark catches, particularly of age-0 individuals (Parsons and Hoffmayer 2007) Catches of such sharks in this study were highest during summer months The...
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Tài liệu Báo cáo khoa học: "Automatic Identification of Pro and Con Reasons in Online Reviews" ppt
Ngày tải lên :
20/02/2014, 12:20
... Proceedings of International Conference on Computational Linguistics (COLING-2000) Saarbrücken, Germany Hu, Minqing and Bing Liu 2004 Mining and summarizing customer reviews" Proceedings of the ... mixture of opinions and facts, making identifying them in online reviews a distinct problem from opinion sentence identification Finally, we also apply the resulting system to another review data in ... researchers in Computational Linguistics define an opinion for their studies It is difficult to define what an opinion means in a computational model because of the difficulty of determining the unit of...
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Báo cáo khoa học: Autoregulatory binding sites in the zebrafish six3a promoter region define a new recognition sequence for Six3 proteins potx
Ngày tải lên :
29/03/2014, 08:20
... showed specific binding to Six4 and other members of the Six1 ⁄ and Six4 ⁄ subfamilies [17] In further investigations of the DNAbinding properties of murine Six3, in vitro binding site selection ... identical to the corresponding DNA -binding domain of orthologous Six3 proteins in other vertebrates, displayed differential binding affinities for various sites containing a TAAT core motif, and ... previously reported Six3 -binding sequence (TGATAC), we observed similar binding affinity, suggesting that the Six3 HD has several binding modes with distinct DNA -binding specificities Using several experimental...
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Báo cáo khoa học: Structural stability of the cofactor binding site in Escherichia coli serine hydroxymethyltransferase – the role of evolutionarily conserved hydrophobic contacts potx
Ngày tải lên :
30/03/2014, 02:20
... directly involved in the proper positioning or stabilization of the active site or PLP -binding residues Its location rather suggests a role in bridging different structural regions of the protein in ... PLP -binding properties of the monomeric denaturation intermediate The cofactor binding properties of monomeric wildtype and mutant enzymes in 2.3 m urea (dissolved in the same buffer used in the ... are involved in cofactor binding, substrate binding and catalysis (Fig 1) On the other hand, the mutations did not affect either the capability to bind the cofactor or the catalytic activity of...
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Báo cáo khoa học: " Sequence analysis of the S1 glycoprotein gene of infectious bronchitis viruses: identification of a novel phylogenetic group in Korea" docx
Ngày tải lên :
07/08/2014, 20:23
... they may in fact consist of a mixture of two different kinds of viruses Distinguishing between these possibilities will require further characterization using cloned viruses The evolution of IBV ... [15,20] Widespread uses of various vaccines made from heterologous IBVs in the field may also exert pressure resulting in the increase of new genetic variants in Korea In summary, 12 Korean field ... K545-02 Table Comparison of the nucleotide and deduced amino acid sequences of the S1 glycoprotein gene of 12 Korean IBV isolates and non-Korean strains Percent similarity-amino acids 10 11 12 13...
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Báo cáo y học: "4th meeting of the EU research network EUROME: From the identification of genes and cellular networks in murine models of arthritis to novel therapeutic intervention strategies in rheumatoid arthritis, London, UK, 9 March 2004" pot
Ngày tải lên :
09/08/2014, 01:23
... More importantly, knocking out the expression of gelsolin, an actin -binding protein with filament-severing properties found downregulated in RA, resulted in mild exacerbation of the arthritic phenotype ... factor H-like protein 1) to its surface Binding is mediated by two types of plasmidencoded protein, class I (20 kDa proteins) and class II (27.5–35 kDa proteins) Outer surface protein E was described ... activation resulting in innate cell mediator activation and the production of inflammatory cytokine interleukin-1 and TNF-α, leading to joint destruction In recent studies, mice depleted of macrophages...
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Báo cáo y học: " COMIT: identification of noncoding motifs under selection in coding sequence" pot
Ngày tải lên :
09/08/2014, 20:21
... microRNA binding in plant coding regions We next examined microRNA binding in animal coding regions While animal untranslated regions have been studied extensively for microRNA binding, animal coding ... capable of detecting coding motifs functional at the DNA level MicroRNA binding motifs Finally, we considered whether COMIT was able to detect microRNA binding sites in coding regions We first examined ... binding sites in human protein- coding sequence BMC Bioinformatics 2006, 7:419 Robins H, Krasnitz M, Levine AJ: The computational detection of functional nucleotide sequence motifs in the coding...
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