... of GM-CSF (10 ng/ml) The purity of bone marrow derived DC was more than 95% as determined by flow cytometry using anti-CD11c and anti-I-A antibody (not shown) With respect to the mechanisms by ... DC, as assessed by flow cytometry DCs were propagated from bone marrow as described by Inaba et al [30] Using magnetic beads, bone marrow cell suspensions were first depleted of B cells (with ... suggest the possibility of unique pathways and requirements for Ag presentation by DC separations as before [24,25] Briefly, spleen cell suspensions were first depleted of B cells using Dynabeads conjugated...
... was varied in range of 3.2 to 4.8 g/l with 0.25 g/l of CaOCl2 added The effect of concentration of CaO to the odor of wastewater was shown in Table When the concentration of CaO increase, wastewater ... can be explained by precipitation of Ca2+ with soluble pollutants Effect of CaOCl2 concentration Wastewater was added with a range of 40 200 mg/l of CaOCl2 with fixed 4.4 g/l of CaO From Table ... of TCVN 5945-2005 After combination of results in tables 2, and we demonstrate that 120 mg/l of CaOCl2 and g/l of CaO is the best condition for treatment of litre wastewater After treatment by...
... instead of Azo (Fig 1) Clear-cut on–off photoregulation of DNA transcription was obtained because of the efficient suppression of T7 RNA polymerase (RNAP) binding to the DM-azo in the trans form By ... thermal stability of the cis form between Azo and DM-azo (A) Illustration of the reversible photoisomerization of Azo and thermal isomerization of cis-Azo (B) Quantitative calculation of cis-form content ... on–off photoregulation of DNA transcription is promising as a photoswitched supplier of RNA fuel for driving nanodevices [5] Experimental procedures Materials Oligonucleotides consisting of only...
... survival data using the coxph function Hazard ratios were calculated as the ratios of risk by the increase or decrease of log2 PLA unit (2-fold increase or decrease in plasma concentration of a biomarker) ... of tumor markers consisting of CEA and CA125 can prognostically stratify cases of unresectable pancreatic cancer Conclusions This study of 52 cases and 43 controls is the largest sample set of ... survival of 52 cases of advanced, unresectable pancreatic cancer Cohort divided into tertiles by CEA plasma levels measured by proximity ligation assay Red line denotes highest 33% by CEA plasma...
... by each method are given in Table It is observed that the lowest error is achieved by the first 64 features with an error level of 3.5% by our proposed approach For the method of [7], 43 out of ... different behavior The signals were first represented byusing LCP over the pyramidal tree structure The pyramidal tree was pruned byusing the algorithm of Section 3.3 and the adaptive time segmentation ... is supported by National Science Foundation (NSF) and by the Project EEEAG-106E057 and Program 2214 of National Scientific Research Council of Turkey REFERENCES 50 100 150 Number of features 200...
... image processing of numeric images of Petri dishes Phys Med Biol 2003, 48:1551-63 Dahle J, Kakar M, Steen HB, Kaalhus O: Automated counting of mammalian cell colonies by means of a flat bed scanner ... microscope) The software provides three independent parameters: the gray level, the maximum size of one colony (defined by the area) and another parameter that considers the distribution of the gray ... (microscope) which has after all an interindividual difference of around 2% Another argument is the fact Figure Screenshot of the program Screenshot of the program Left panel: the scanned flask, the circle...
... Resolver Percentage of subjects 100% Positive EBC pH Profile Negative EBC pH Profile Figure Multi-sample EBC pH profiles of controls and patients Multi-sample EBC pH profiles of controls and patients ... accomplished by Mann-Whitney Rank Sum, and the number of low EBC pH values for each group compared by Chisquared analysis The ability of the system of EBC collections and resulting EBC pH profile in ... subject to develop an EBC pH profile, and this profile allows for much greater sensitivity of the procedure by enhancing the likelihood of finding a correlation of cough with acidity if it is...
... the estimation of slopes of the profiles, which permits the replacement of differentials with the estimated slopes at many time points and consequently the conversion of systems of differential ... itself Because of the inherent nonlinearities, the task is hampered by trapping of the search algorithm in local minima, lack of convergence, or a convergence speed that makes inference of larger ... the case of S-systems, it would also be feasible to use steady -state data from multiple experiments with different values of independent variables Because the steady -state equations of S-systems...
... satisfaction of the dissertation requirements for the degree of Doctor of Philosophy January 2006 Prof Hiroshi Matsui Date Chair of Examining Committee January 2006 Prof Gerald Koeppl Date Executive Officer ... THE CITY UNIVERSITY OF NEW YORK ii Abstract SYNTHESIS OF INORGANIC NANOWIRES BYUSING PEPTIDE NANOTUBES AS THE TEMPLATES VIA BIOLOGIC RECOGNITION by Lingtao Yu Adviser: Professor Hiroshi Matsui ... The properties of the outer surface and the internal diameter of peptide nanotubes can be adjusted simply by the choice of the amino acid side chain functionalities and the size of the peptide...
... concentrations of NaCl Figure shows that the corrosion current of all test samples of PANi coated steel is less than that of uncoated steel Figure shows that the corrosion rate has been decreased by increasing ... resistance of buried steel by coating steel with a layer of polyaniline (PANi) The galvanostatic technique was used for forming the PANi layer for its simplicity and ease of industrial application using ... data are the average of 50 scans of each element analysed The surface composition in atomic percentage was calculated using the element relative cross sectional area as supplied by the XPS manufacturer...
... TEACHING PRONUNCIATION OF ENGLISH CONSONANTS BYUSING CONTRASTING AND SUBSTITUTING TECHNIQUES is the result of my own research of the degree of Master of Arts at the University of Languages and International ... hand in front of their mouth to feel the puff of air Also, in teaching the pronunciation of /θ/, the place of articulation and manner of articulation should be contrasted This sound is often confused ... 26 3.1 Results of the placement test 26 3.2 Discussion of the placement test findings 28 3.3 Results of the evaluation test 31 3.4 Discussion of the results of the evaluation...
... Horrible levels of vulnerability • Exposure of data and professionalization of cybercrime • Venture community pulls up stakes • Lawmakers stake out turf and arrive Current Stateof Security • Industry ... Current Stateof Security • Some Extrapolation Some History • The early days of computer security: • Audit function - oversight • Mainframe usage accounting and system log analysis • Often an ... • IDS researcher / CEO of NFR 1997 • CSO, consultant, teacher, writer Disclaimer • This is an “industry” view • Much of what I’m talking about will ripple in the form of changes to: • Budgets...
... on Page of 20 International Finance Regulation of Interest Rate by the State Bank of Vietnam (SBV) interest rate-ceiling The State Bank has thought that joint stock banks as well as state owned ... on loans on the basis of market capital supply – demand and the creditability of borrowers” Page of 20 International Finance Regulation of Interest Rate by the State Bank of Vietnam (SBV) This ... following figures for the market share of different types of banks in Vietnam, and that of four biggest state- owned banks Share of different types of Banks 21.4% State- owned bank 0.6% Urban Commercial...
... FEBS 6945 Modeling of ANT E Metelkin et al Fig Effect of nigericin on ATPADP steady -state exchange rate mediated by ANT Bar graph of ATPADP steady -state exchange rate mediated by ANT in the absence ... Ap5A State respiration was initiated by the addition of mm K-ADP to the incubation medium State respiration was initiated by the addition of cATR at the indicated concentrations Determination of ... Fig Titration of ATPADP steady -state exchange rate mediated by ANT with cATR and correlation with DWm (A) ATPADP steadystate exchange rate mediated by ANT determined as a function of cATR concentration...
... biochemical identificationusing the API biochemical identification system API Staph (BioMerieux, USA) was used for identificationof S aureus and API 20 A (BioMerieux, USA) for identificationof P ... Amplification of single-stranded DNA and sequencing of four bacterial genes The mixture for PCR was as follows: μl of 10 × PCR buffer, μl of 10 mmol/l dNTP mix; μl of 10 μmol/l 16s-a, μl of 10 μmol/l ... pipe at a speed of μL/ After the reaction completed, the chip surface (precoated with probes) was regenerated by washing with 100 μL of 0.01% SDS and 100 μL of mM HCl at a speed of 50 μL/min To...
... in the mid-portion of the of the aMPV/C/US/Co isolate (Fig 2) by western blot which would recognize any downstream translation products encoded by the N gene and utilization of any secondary start ... sion vector Expression of N1 and N2 open reading frames of avian metapneumovirus type C in transfected eukaryotic cells by an expresExpression of N1 and N2 open reading frames of avian metapneumovirus ... genome by itself cannot fulfill the role of template Pneumovirus infection in cells results in the accumulation of the N protein in cytoplasmic inclusion bodies that can be visualized by immunofluorescence...
... humans However, the main goal of these studies was to identify the onset (and offset) of a specific neural activity, with the aim of triggering stimulation The aim of our work was to investigate ... ("VF") of regulated intensity by touching the plantar skin with a von Frey filament (Stoelting Co, Illinois) (2) proprioceptive stimulus ("Proprio") provoked by means of complete passive flexion of ... duration of stimuli were identified by experimenter's bottom pressure in synchrony with start and end of stimulus application, while VF touch stimulation was also recorded by means of a pressure...
... to the root of contemporary strains, suggesting that VP0 may have been acquired by a more recent ancestor of the group by recombination Discussion Enteritis is caused by a spectrum of viruses ... further studies of full genomes of currently circulating isolates of HPeV1 have been conducted A finding of recombination in principle is not surprising given the propensity of picornaviruses ... set of structural protein genes by recombination in the 5'-proximal part of the non-structural protein genes, close to the VP1/2A border Because the VP3 portion of BNI-788st and its group of relatives...
... biochemical identificationusing the API biochemical identification system API Staph (BioMerieux, USA) was used for identificationof S aureus and API 20 A (BioMerieux, USA) for identificationof P ... Amplification of single-stranded DNA and sequencing of four bacterial genes The mixture for PCR was as follows: μl of 10 × PCR buffer, μl of 10 mmol/l dNTP mix; μl of 10 μmol/l 16s-a, μl of 10 μmol/l ... pipe at a speed of μL/ After the reaction completed, the chip surface (precoated with probes) was regenerated by washing with 100 μL of 0.01% SDS and 100 μL of mM HCl at a speed of 50 μL/min To...
... in the mid-portion of the of the aMPV/C/US/Co isolate (Fig 2) by western blot which would recognize any downstream translation products encoded by the N gene and utilization of any secondary start ... sion vector Expression of N1 and N2 open reading frames of avian metapneumovirus type C in transfected eukaryotic cells by an expresExpression of N1 and N2 open reading frames of avian metapneumovirus ... genome by itself cannot fulfill the role of template Pneumovirus infection in cells results in the accumulation of the N protein in cytoplasmic inclusion bodies that can be visualized by immunofluorescence...