identification+of+genes+with+altered+expression

... known expression profiles in Heatmap of SAGE tag counts for genes with known expression profiles in pancreas development Tags for genes with well characterized expression profiles in pancreas ... lists the full categorization of each of these genes The percentage of genes with each staining profile in each of the SAGE tag K-means cluster is shown number of genes not detected was highest ... Relative expression level 0.24 Figure SOTA clustering of temporal expression profiles from qRT-PCR analysis of 44 genes in pancreas development SOTA clustering of temporal expression profiles from...

Ngày tải lên: 14/08/2014, 20:22

... the putative function of the 32 genes Page of 10 tested by qPCR and their pattern of expression, we suggested in the figure a possible representation of involvement of these genes dureint the interaction ... resistance/susceptibility genes were selected and their expression profiles by qRT-PCR (Figure 2) Quantitative RT-PCR analysis of the same cDNAs used for AFLP analysis (2 and 48 hpi) confirmed the profile of expression ... (data not shown) The pattern of expression of of these genes [(Chalcone syntahse (115), and Chalcone isomerase (98)] confirms the results of Venisse et al (2002) These genes could possibly be useful...

Ngày tải lên: 12/08/2014, 03:21

... number of genes obtained was very limited, the largest group of genes (30%) was signaling/transcription related genes followed by genes with unknown (26%) and defense functions (22%) One of the ... Seventeen of the 23 genes showed statistically significant increased expression with real-time PCR in the WT relative to evg (Figure 2) by ANOVA with the Mann-Whitney test The expression of two DAM genes, ... expected we did not detect expression of these genes in evg tissue by real-time PCR Gene expression in SD conditions We measured the expression response of the 23 genes identified above to the...

Ngày tải lên: 12/08/2014, 03:21

... architecture of cancer through the control of environmental exposure leading to tumorigenesis, which cannot be done with human populations [11] A recent success in constructing a haplotype map of the ... with two alleles M and m is associated with a QTL with two alleles A and a that determines the clonal expansion of a cancer with age The allele frequencies of marker allele M and QTL allele Q are ... given parameter values of cancer estimated values (broken) with different heritabilities (H2) for a sample size of n = aa, Curves for the number of cancer clones changing with age, determined by...

Ngày tải lên: 13/08/2014, 16:21

Ngày tải lên: 14/11/2014, 13:05

... co-cultured with either Th1 or Th2 cells display different gene expression patterns A total of 115 differentially expressed genes were identified, which might be involved in the regulation of Th cell ... in the rat as a model of RA A joint-specific disease with many similarities to human RA develops after a single injection of pristane subcutaneously [3] This contrasts with murine pristaneinduced ... importantly, knocking out the expression of gelsolin, an actin-binding protein with filament-severing properties found downregulated in RA, resulted in mild exacerbation of the arthritic phenotype...

Ngày tải lên: 09/08/2014, 01:23

... surgical procedures with informed consent from seven patients with end-stage osteoarthritis, in accordance with the terms of the Ethics Committee of the University of Ulm The age of the donors ranged ... After 14 days of differentiation, the gene expression profile of osteoblast markers was investigated For OC cells and bone marrow MPCs we detected a strong signal for expression of genes for all ... recapitulation of development such as expression of procollagen type IIA [29] Possibly, the activation of MPCs may contribute to the observed expression of this alternative splice variant A misguiding of...

Ngày tải lên: 09/08/2014, 01:23

... the expression patterns of genes in a specific tissue or at a specific developmental stage (Gueguen et al., 2003) Full-length cDNAs are also useful tools for the analysis of expression profiles ... betaine aldehyde dehydrogenase (BADH2) (1-the number of ESTs matched in GenBank, FJ228482-the accession number of genes of Fig The PCR products of cDNAs cloned randomly from the full-length cDNA ... 2003) The availability of stresstolerance genes for vegetable crop improvement is one of the key factors for the success of gene transfer Solanum torvum, a wild relative of eggplant, has been...

Ngày tải lên: 11/11/2016, 15:59

... in lung of rats injected with high-dose of vitamin A over a period of days, the overexpression of c-Jun, produced a significant increase in the cyclin D1 expression, a positive regulator of G1–S ... followed the pattern of p53 and c-Jun gene expression showed in Figs and Immunoprecipitation of complex RAR–DNA To elucidate the mechanism of this pattern of expression, the binding of RAR to c-Jun ... control rats; H, rats with a high-dose of vitamin A over a period of days Fig Diagrammatic representation of the changes induced in the control of proliferative genes by vitamin A Within the nucleus,...

Ngày tải lên: 08/03/2014, 08:20

... al.: Down-regulation of UHRF1, associated with re -expression of tumor suppressor genes, is a common feature of natural compounds exhibiting anti-cancer properties Journal of Experimental & Clinical ... downregulation of UHRF1 with subsequent increase of p16INK4A gene expression The down-regulation of UHRF1 is probably driven by p53 and/or p53 This is leading to an inhibition of tumor vascularization ... promoters of TSGs, i.e., p16INK4A [44], and that UHRF1 interacts with DNMT1 and regulates its expression [49], it is likely that the RWPs-induced down-regulation of UHRF1, with subsequent decrease of...

Ngày tải lên: 10/08/2014, 10:21

... diagnosed with stage IV of the cancer, expression of the serum APPs did not conform to the altered progression changes The expression of HAPc and AATf did not demonstrate any correlation with all ... to monitor the expression of the serum proteins with progress of EOCa In the present study, we demonstrated the correlative expression of ACT, CLU, HAP, AAT and LRG in patients with EOCa in accordance ... protein profiles of normal control women Unlike the initial three stages of EOCa, the expression of ACT, CLU, HAP, AAT and LRG in sera of EOCa patients in stage IV did not conform to the altered...

Ngày tải lên: 10/08/2014, 22:20

... identification of 52 genes displaying maternal-specific expression (MEGs) The maternal specific expression of some of these MEGs may be due to genomic imprinting Within these 52 maternally expressed genes, ... (Seed Genes Network), the extent of imprinting did vary, with the C24 and Bur-0 alleles displaying a greater extent of imprinting when paternally inherited ICRs of imprinted genes often overlap with ... of an imprinted gene in the endosperm [17] The choice of transcript profiling platform is an important consideration for identification of novel imprinted genes Microarrays are dependent on genes...

Ngày tải lên: 11/08/2014, 11:21

... stress-related genes were selected for expression analysis to identify genes with transcript levels that correlated with sugar content in culm and leaf tissues Expression patterns of a sub-set of these genes ... analyses of changes of sucrose during sugarcane growth and development [15] Expression of stress-related genes The relative abundance of transcripts of the 51 genes in different parts of mature ... of a microarray-based expression analysis of 30 sugarcane genotypes with variation in sugar content (measured as Brix) with that of an earlier study [10] of signal transduction-related gene expression...

Ngày tải lên: 11/08/2014, 11:21

... show high expression of genes near the top of the panel and moderate or low expression of genes below, while leaves (labeled 5) show low or moderate expression of genes near the top of the panel ... the microarray data, a set of 21 tissueenriched genes were selected for RT-PCR analysis The RT-PCR expression pattern of 18 out of the 21 genes was consistent with that of the microarray experiments ... the regulation of shoot apical meristem activities and morphogenesis of shoot organs [34-37] Of Figure Dendrogram of 2566 tissue-specifically expressed genes in the five tissues of O longistaminata...

Ngày tải lên: 11/08/2014, 11:21

... technology and qRT-PCR associated with semigamy in cotton In this study, over 2,000 differentially expressed genes associated with semigamy were identified with 127 of those genes being commonly differentially ... about the molecular genetics and gene expression of semigamy Therefore, the objective of this study was to identify differentially expressed genes associated with the semigametic genotype using ... example, among 81 genes with the same GeneBank accession numbers in both tissues, most genes (77) were consistently down-regulated in both anther and ovule tissues of 57-4 and two genes were consistently...

Ngày tải lên: 11/08/2014, 11:21

... all of the three BAC clones was used as a hybridization probe All of the cultivars examined contained Glo-3 genes Based solely on the number of bands, there are at least two copies of Glo-3 genes ... genes, Glo-3B and Glo-3C These findings, identification of the genes whose homologues code for wheat proteins potentially associated with the pathogenesis of T1D, prompt consideration since they ... Triticum (total of 1,106,281 sequences, 22.03.09) EST sequences with minimum identity of 86% and minimum E value of 1e-07 were chosen Presence of the conserved domains was screened with NCBI CDD...

Ngày tải lên: 12/08/2014, 03:20

... profile of a bona fide syncytin gene, with high levels of expression in the placenta and very limited expression in other tissues Expression in the placenta decreases with gestational age, with ... transfections of a set of A23 cells with an empty vector (none) or an expression vector for either the syncytin-Ory1, syncytin-1 or syncytin-2 protein together with an nls-LacZ gene -expression vector, ... transfected by using the Lipofectamine kit (Invitrogen) with 0.2 μg of either the syncytin-Ory1 expressing or an empty vector, supplemented with 0.2 μg of a LacZ -expression vector (pCMV-β, Clontech)...

Ngày tải lên: 12/08/2014, 23:22

... control of hepcidin expression [23], in the B6 strain Finally, and worthy of note within the context of modifiers of iron loading severity, wildtype D2 mice have significantly lower expression of ... increased expression of the mucin (Muc3) and spermin synthase (Sms) genes, which encode proteins that both may modulate iron uptake [18,19] Changes in expression of genes encoding proteins of iron ... over-representation of functional categories within the different gene clusters from Figure Categories found to be enriched within the clusters of genes similarly regulated in the liver of Hfe-/- compared with...

Ngày tải lên: 14/08/2014, 08:20

... efficient for the identification of novel transcripts Microarray analysis of genes involved in neural induction (that is, dorsal genes) allowed the identification of 14 novel transcripts out of 32 that ... Wnt signaling on expression of novel transcripts Effect of5 Effect of Wnt signaling on expression of novel transcripts X tropicalis embryos were injected at the two-cell stage with control and ... tags were identified Five of them map to genes with known dorso-ventral expression and the frequency of appearance for these tags in each library is in agreement with the expression described by...

Ngày tải lên: 14/08/2014, 21:20

... The absence of stainable iron is the “gold standard”, for diagnosis of IDA Marrow examination shows, in addition to the absence of hemosiderin iron, a decrease in the proportion of sideroblasts, ... 2–3% of patients presenting with IDA have coeliac disease [3-6, 11] Iron deficiency anemia is considered as an alarm sign for the presence of possible GI malignancies, and inadequate evaluation of ... evaluation of patients with IDA may delay the diagnosis of GI tumors especially colorectal cancer [12] In this study, we aimed to evaluate the diagnostic yield of endoscopy in patients with IDA and to...

Ngày tải lên: 25/10/2012, 11:18