identification of cooked bone using tem imaging of bone collagen

... Principle of Split-Ubiquitin Screening System Studies have shown that the use of yeast two hybrid system, which is a genetic assay for in vivo detection of interactions of proteins, enables identification ... located at the C terminus of Cub will be cleaved off and result in downstream reactions, which is the activation of LacZ and HIS3 reporter genes [7] 3.2 Identification of Cellular Membrane Proteins ... Stagljar I Identification of Table Summary of Cellular Membrane Proteins Interacting novel ErbB3-interacting factors using the split-ubiquitin membrane with HBsAg yeast two-hybrid system Genome...

Ngày tải lên: 02/11/2012, 11:08

... optimum approximation of random field The main idea of the POD is to find out a set of orthogonal basic vectors which can expand a multi-variate random process into a sum of products of these basic ... very important role in the identification of random pressure field due to their dominant energy contribution and frequency containing of hidden physical events of system 5.2 Analysis on spectral ... Firstly, effects of basic and cumulative covariance modes on the synthesis of the unsteady pressure fields, as well as role of the first covariance mode on the identification of these pressure...

Ngày tải lên: 05/03/2014, 14:20

... of tumor markers consisting of CEA and CA125 can prognostically stratify cases of unresectable pancreatic cancer Conclusions This study of 52 cases and 43 controls is the largest sample set of ... AE: Multianalyte profiling of serum cytokines for detection of pancreatic cancer Cancer Biomark 2005, 1:259-269 Page 11 of 12 (page number not for citation purposes) Journal of Translational Medicine ... profiling of putative pancreatic cancer biomarkers After applying advanced statistical methods to this dataset, we identified a panel of three biomarkers that exceed the diagnostic accuracy of...

Ngày tải lên: 18/06/2014, 15:20

... (HL), toe off (TO) and heel strike (HS) using a relatively straight-forward prediction algorithm It was felt that this proof of concept study was necessary to assess the suitability of a textile-based ... effect of walking speed Gait Posture 26, 323–326 (2007) doi:10.1016/j.gaitpost.2006.09.077 JM Hausdorff, Z Ladin, JY Wei, Footswitch system for measurement of the temporal parameters of gait ... between resistance and deformation One of the primary causes of this non- linearity is the viscoelatic properties of the textiles which results in a number of phenomena, such as velocity dependent...

Ngày tải lên: 19/06/2014, 08:20

... humans However, the main goal of these studies was to identify the onset (and offset) of a specific neural activity, with the aim of triggering stimulation The aim of our work was to investigate ... filter with cutoff frequencies of 10 Hz and kHz), digitized at 20 kHz (PowerLab) and fed into a PC running Chart v5.5 (AD Instruments) Datasets consisted of ten applications of every type of stimuli ... increasing the number of stimuli to be identified on groups of two and three, until finding an acceptable percentage of classification The influence of different parts of the proposed signal...

Ngày tải lên: 19/06/2014, 08:20

... rapid wear of the AC, and consequently reduction of the loading capacity of AC as a result of progressive release of proteoglycans from the cartilage [22] Therefore, study of the 3D collagen network ... observations of the collagen orientation Using computer software VoxBlast (VayTek, Inc, USA), the image stack of the collagen fibres was reconstructed as a 3D image for visual inspections Using F900e, ... prevention of the initial lesion of AC surface and increase of the durability of the AC This is consistent with the fact that loss of the most superficial layer of AC accelerates worn off AC [13]...

Ngày tải lên: 20/06/2014, 01:20

... read using the Cyclone Phosphor Imaging System The regions of interest of the same size were drawn and analyzed on the brain regions rich in a4b2 nicotinic receptors using the OptiQuant software, ... hemisphere using the Leica 1850 cryotome and were exposed to phosphor films overnight The films were read using the Cyclone Phosphor Imaging System and were analyzed using the Optiquant software ... and a significant amount of 18 Fnifene parent remained in the blood after 90 of the PET study The absence of lipophilic metabolites was also confirmed using brain extracts of rats injected with...

Ngày tải lên: 21/06/2014, 02:20

... small speculated tumour in the middle of the right breast, left side of figure 14 The aim of this study is to develop a feasible interpretive software system which will be able to detect and classify ... kinds of eye diseases [33] HOS is known to be efficient as it is more suitable for the detection of shapes The aim of using HOS is to automatically identify and classify the three kinds of mammogram ... predictive accuracy (PPA) of SVM and GMM classifiers TP = number of true positive specimens FP = number of false positive specimens FN = number of false negative specimens TN = number of true negative...

Ngày tải lên: 14/07/2014, 11:49

... total volume of 100 µl containing mM MgCl2, 0.2 mM each dNTPs, 20 pmol of each PCR primer, U of Taq DNA polymerase (Takara, Japan), and 10 µl of templates Temperature conditions consisted of an initial ... (7/1,682) 0.59 (10/1,682) 0.65 (11/1,682) a No of positive/No of samples examined No of negative/No of samples examined b Table Antibiotic resistance profiles of isolated E coli O157:H7 Antimicrobial ... index of discriminatory ability of typing systems: An application of Simpson’s index of diversity J Clin Microbiol 1988, 26, 2465-2466 24 Karmali MA, Petric M, Bielaszewska M Evaluation of a microplated...

Ngày tải lên: 07/08/2014, 18:21

... presence of the PCR fragments was visually scored with the help of fragment size standards To confirm the accuracy of the visual reading and to evaluate the size of the alleles, two samples of 21 ... considered of less importance to reject a stand of French origin when it was of French origin (type I error) than it was to accept a stand as being of French origin when it was not The probability of ... (table IV) Using the two selected loci, only one of the λ stands was found to be of NW Iberian origin, while all the remaining stands were of French origin Furthermore, the stand of putative...

Ngày tải lên: 08/08/2014, 14:20

... recapitulation of development such as expression of procollagen type IIA [29] Possibly, the activation of MPCs may contribute to the observed expression of this alternative splice variant A misguiding of ... Isolation and characterization of size-sieved stem cells from human bone marrow Stem Cells 2002, 20:249-258 Shur I, Marom R, Lokiec F, Socher R, Benayahu D: Identification of cultured progenitor cells ... terms of the Ethics Committee of the University of Ulm The age of the donors ranged from 55 to 89 years (mean 74 years) The diagnosis was based on clinical and radiological criteria None of the...

Ngày tải lên: 09/08/2014, 01:23

... residues by deimination of arginine residues of proteins Isoforms and of PAD were expressed in mononuclear cells isolated from SF [15] These data suggest that presence of citrullinated proteins ... maturation of B cells at the site of inflammation However, it is poorly understood what kind of proteins are citrullinated and recognized as targets of autoantibodies in the synovial tissue of patients ... fifty micrograms of the extracts was applied to the drystrip gels for detection of antigens and 500 μg for identification of proteins by mass spectrometry (MS) IEF was performed using Ettan IPGphor...

Ngày tải lên: 09/08/2014, 08:23

... quality of DNA (high molecular weight) and absence of RNA contamination The DNA quality and quantity were then determined using a NanoDrop® spectrophotometer (NanoDrop Technologies, Inc., now part of ... were performed using GTYPE GeneChip Genotyping Analysis Software version 4.0.0.22 and GCOS GeneChip Operating Software version 1.4.0.036 The GTYPE GeneChip Genotyping Analysis software generates ... determined using software developed in-house by personnel at JKA Genomics (Oklahoma City, OK, USA) GeneChip Genotyping 4.0 software output was used for relative quality control assessment of detection...

Ngày tải lên: 09/08/2014, 14:20

... level of the 47th codon (F et al , 1988, submitted), was URET radiolabelled with (( to a specific activity of 10 dpm/ f.Lg, using the « multi9 2p)dCTP Xl prime DNA labelling system RPN 1601 » of ... of patterns found after hybridization of Hind III digests 0.8 kb fragment present in all samples, a polymorphism made up of of approximately 2.2, 3.3 and 5.5 kb may be observed A comparison of ... Those observations suggested a possible identification of alleles K and K by the technique of restriction fragment length A -Cn l -Cn polymorphism (RFLP) using enzymes Hind III and Taq I II Materials...

Ngày tải lên: 09/08/2014, 22:22

... along with expression of one of the other markers Using this panel of markers, 20/56 (36%) of patient blood samples had detectable levels of gene expression for at least two of the markers including ... classification system determines stage of disease using tumour size, involvement of lymph nodes, and distant metastasis, the relationship of overall stage of disease and positive expression of markers ... used as the template in a single round of PCR amplification with 200 nM of each gene specific primer pair (Table 1), U of HotStarTaq (Qiagen, Hilden, Germany), 2.5 mM MgCl2, and 200 μM of each deoxynucleotide...

Ngày tải lên: 10/08/2014, 22:20

... using Haploview [21] The frequencies of common haplotypes (> = 1%) were compared between non-responders and responders using odds ratios, with 95% confidence intervals calculated using Page of ... allowing breaking up of the large haplotype block and thus allowing inference of the relative effects of each SNP The task of demonstrating the basis for causality, even of haplotypes, is not ... best positive predictive value of treatment failure This haplotype was conserved from the 3’ end of the gene to 8kb upstream of the 5’ end of the gene in people of European descent This causal...

Ngày tải lên: 11/08/2014, 12:21

... 43.3% of clenching, and 24.4% of tapping activities based on the count of events; whereas 56.8% of grinding, 37.4% of clenching, and 5.8% of tapping were registered based on the length of events ... laboratory and participated in the analysis of raw data of EMG, EEG, and ACC KS participated in the development of new analyzing system of sleep bruxism using EMG and ACC KT participated in collecting ... bruxism and analyzed the behavior of sleep bruxism in 19 volunteers The new analyzing system of bruxism has two major advantages First, the combined system of EMG and Acc provides clear and easy...

Ngày tải lên: 11/08/2014, 20:20

... Analysis of four of these BAC clones containing 11 identified PPO genes provided evidence of a potential cluster of six distinct PPO genes within 190–510 Kb (Figure 5) The full sequence of BAC ... Diagram 5of cluster of PPO genes detected on four separate Diagram of cluster of PPO genes detected on four separate BAC clones The four BAC clones have been aligned based on detection of specific ... families Phylogenetic tree of coding DNA sequences of selected PPO Phylogenetic tree of coding DNA sequences of selected PPO genes and gene families DNA sequences of all selected plant species...

Ngày tải lên: 12/08/2014, 03:20

... is the first identification of a genetic factor underlying resistance to the RSNV disease In order to better elucidate the bases of genetic control of RSNV resistance, fine mapping of this region ... the list of 18,000 SSRs published in IRGSP (2005) Polymerase chain reactions (PCR) were performed in a total volume of 15 μL containing 20 ng/μL of DNA template, 1X PCR buffer, 2.5 mM of MgCl2 ... separated using 6% denaturing polyacrylamide gel followed by silver staining, as described in the Promega Technical Manual [66] Selection of a subset of CSSLs Selection of a subset of introgression...

Ngày tải lên: 12/08/2014, 03:21

... activity Proceedings of the National Academy of Sciences of the United States of America 1992, 89(15):7208-7212 35 Ohmiya A: Characterization of ABP19/20, sequence homologues of germin-like protein ... Page of 11 Figure Stem growth and potential re-growth of WT and evg plants after transferring to SD Stem growth (increase in length) was measured one week prior and eight weeks after transfer of ... signal in pattern formation in plants Proceedings of the National Academy of Sciences of the United States of America 1996, 93(17):9282-9286 Page 11 of 11 42 Eliasson A, Gass N, Mundel C, Baltz R,...

Ngày tải lên: 12/08/2014, 03:21