identification isolation characterization and banking of human dental pulp stem cells

... (2004) Isolation of mesenchymal stem cells of fetal or maternal origin from human placenta Stem Cells 22, 1338–1345 Bieback K, Kern S, Kluter H & Eichler H (2004) Critical parameters for the isolation ... system will provide a valuable tool, in addition to primary hepatocytes, for study of the molecular basis of the regenerative and developmental processes of hepatic cells in vitro Isolation of ... development of in vitro test systems Although primary human hepatocytes are the best source of cells for such systems, their use for this purpose is limited Transcriptome in hepatic induction of AT-MSCs...

Ngày tải lên: 30/03/2014, 04:20

... benign samples PB performed stem cell isolations from tumors CH performed culturing and isolation of stem cells, and NF-κB experiments JLL performed the RTPCR detection of the TMPRSS:ERG fusion ... cancer cells PTL induces apoptosis in primitive cancer cells Percent viability of prostate cancer cells and cells from a patient with BPH treated with increasing concentrations of PTL Cells were ... further study of larger samples of prostate cancer stem cells from different classes of therapy-resistant and Gleason tumors is warranted Despite short-term culturing, to expand the stem cell population,...

Ngày tải lên: 14/08/2014, 08:21

... Language, Research Systems, Inc), to measure the speed of the cells and analyze the behavior of the cells motility Figure 3.3 shows a screen print image of the interface of the IDL program The ... and constant support during this work I am also thankful to Shao and Jeroen for their guidance through the early years of chaos and confusion And the close cooperation from A/Prof Ge Ruowen and ... Vein Endothelial Cells (HUVEC, Figure 1.1 [4]) Figure 1.1: Micrograph of HUVE Cells plated on a flat substrate 1.2 Endothelial Cells and Angiogenesis What are Endothelial Cells and why we study...

Ngày tải lên: 26/11/2015, 12:55

... reported for GLUT1 [20] and GLUT3 [21] Comparative analysis of gcGLUT to members of the class I (human GLUT1, GLUT2, GLUT3 and GLUT4), class II (human GLUT5) and class III (human GLUT10) To study ... which contained 20 ng of first strand cDNA, · PCR buffer (20 mM Tris/HCl pH 8.4, 50 mM KCl), 0.2 lM of each primer, 1.5 mM MgCl2 and 0.2 mM of dNTPs PCR Construction and screening of a grass carp genomic ... performed on first-strand cDNAs that were reverse transcribed from total RNA by use of Superscript II reverse transcriptase (Invitrogen) and consisted of 20 ng of first strand cDNA, · PCR buffer...

Ngày tải lên: 17/03/2014, 03:20

... standardization and testing were carried out on separate sets of images, two for training and 12 for testing The number of samples used for standardization was 4, and 10 for the A, B, and C test functions, ... Suitable approaches allowing characterization and quantification of the maturation of virus particles and their intracellular translocation would facilitate objective studies of these phenomena employing ... development of an automated system to assist in the identification of virus particles in electron micrographs As a model, we have used fibroblasts infected with human cytomegalovirus (HCMV), a virus of...

Ngày tải lên: 20/06/2014, 01:20

... Isolation of HPTC and characterization of isolated and commercial HPTC 50 4.1.1 Isolation of HPTC 50 4.1.2 Characterization of HPTC by immunoblotting 51 4.1.3 Characterization of ... containing 0.1 mM of 3-isobutyl-1-methylxanthine (IBMX) (Merck) and exposure of cells to 100 nM and 1000 nM of human PTH (Sigma-Aldrich) and human AVP (SigmaAldrich) for 30 minutes at 37°C The cells were ... (p-values of < 0.05) unless stated otherwise 49 Results 4.1 Isolation of HPTC and characterization of freshly isolated and commercial HPTC 4.1.1 Isolation of HPTC HPTC are available commercially and...

Ngày tải lên: 09/09/2015, 10:19

... These samples, and samples of fresh unsulfided and sulfided catalyst, were characterized by nitrogen adsorption and SEM The results of textural characterization by nitrogen adsorption of the catalyst ... related to the intensity of emission of the characteristic Ka line of each element White corresponds to a high concentration of a given element, and black to the absence of this element, while ... Fig SEM-EDX maps of distribution of elements: red mud after h run time The brightness of every pixel of the EDX maps, B, can be quantified on a scale of (black) to (maximum degree of brightness)...

Ngày tải lên: 23/09/2012, 14:46

... Huo et al Characterization of respiratory complex II Fig Sequencing of porcine heart mitochondrial complex II Total RNA was extracted and purified from the fresh porcine heart, and genes of four ... G250, and the four bands corresponding to subunits of porcine complex II were cut out and N-terminal sequenced by the Edman degradation method and amino acid analyzer in the Department of Biology, ... the left, and the positions of molecular weight standards are indicated on lane FEBS Journal 274 (2007) 1524–1529 ª 2007 The Authors Journal compilation ª 2007 FEBS 1525 Characterization of respiratory...

Ngày tải lên: 19/02/2014, 02:20

... spectrometry and the lability of the sulfogroup in mass spectrometry analysis allows recognition of the modification, and differentiation of sulfotyrosine and phosphotyrosine [38] Characterization of the ... mechanisms of incorporation of PTMs in the biosynthesis of conopeptides Appropriate methods of analysis need to be addressed to ensure recognition of these PTMs, if present, in the course of characterization ... characterization of native a-conotoxins Analysis of neuronally active a-conotoxins using HPLC and MS, including identification of post-translational modifications Isolation and identification Standard procedures...

Ngày tải lên: 19/02/2014, 12:20

... mapping On the basis of the assumptions of Hiromi [30] that the intrinsic rate of hydrolysis (kint) in the productive complex is independent of the length of the substrate, Km and Vmax were used ... Hiromi K (1970) Interpretation of dependency of rate parameters on the degree of polymerization of substrate in enzyme-catalyzed reactions Evaluation of subsite afnities of exo-enzyme Biochem Biophys ... PelB contains a few inserted b-strands (1a in Fig 2), and one large insert of 15 amino acids is present before the rst b-sheet of coil 3, which is on the edge of the pronounced substrate-binding...

Ngày tải lên: 20/02/2014, 03:20

... pattern and extent of stimulation of the membrane and soluble yeast PtdEtnPLD activity by Ca2+ and their inhibition by other divalent cations is highly comparable The mechanism of action of Ca2+ ... both wild-type and spo14D cells Future identification of the PtdEtn-PLD gene and analysis of its promoter region will allow examination of its transcriptional regulation by inositol and the possible ... purification of the cytosolic PtdEtn-PLD resulted in rapid loss of activity Ó FEBS 2002 Characterization and regulation of yeast PtdEtn-PLD activity (Eur J Biochem 269) 3823 Table Effect of different...

Ngày tải lên: 22/02/2014, 07:20

... [27,28] The identification and characterization of the molecular targets of DS are of prime importance in order to understand the pharmacological and ⁄ or toxicological effects of this therapeutic compound ... concentrations of DS in vitro and in human cancer cells Mechanistic and kinetic analyses indicate that DS reacts with the active site cysteine residue of NAT1, leading to the irreversible inhibition of the ... (IC50) of 3.3 ± 0.1 lm and a second-order inhibition rate constant (ki) of · 104 m)1Æmin)1 Our work shows that NAT1 is a new molecular target of DS DS impairs the activity of NAT1 in human cancer cells...

Ngày tải lên: 07/03/2014, 02:20

... particular property of human IRP1 The active participation of mitochondrial KGD1p is borne out by the lack of aggregates in a KGD1 mutant (Fig 6) and implies that association of IRP1 and KGD1p eludes ... that in the mitochondria of cells producing c-hIRP1 Human apo-IRP1 is known to precipitate easily and to form aggregates in vitro in the presence of the divalent metals zinc and cadmium [13], which ... consequence of the presence of unfolded or incorrectly folded human IRP1 This implies that formation of inclusion bodies competes with assembly of the [4Fe)4S] cluster in IRP1 In addition, degradation of...

Ngày tải lên: 07/03/2014, 10:20

... clonal expansion of T -cells bearing human Vb3, Vb12, Vb17 and Vb19 [20] Others showed proliferation of human T -cells bearing Vb1, Vb2, Vb3, Vb5.2, Vb12, Vb15 and Vb17 domains and found differences ... Immobilization of the SAgs instead of TCRs yielded a higher binding constant of the former, which was similar for both complexes, wtSSA–Vb5.2 and SSA(C26S)– Vb5.2 Different concentrations of human Vb2.1 and ... hVb2.1hCb2 and hVb1hCb2 (genes kindly provided by U Utz and R P Sekaly, University of Montreal, Canada), were cloned between the NdeI and EcoRI restriction sites of the pET17b expression vector and...

Ngày tải lên: 07/03/2014, 16:20

... picture of the enzyme and should be helpful in a comprehensive understanding of the role of hKAT-I, especially in KYNA biosynthesis in the human brain Experimental procedures Enzyme expression and ... Department of Animal Health and Biomedical Sciences, University of Wisconsin (Madison, WI, USA) and Dr Menico Rizzi (Department of Genetics, University of Pavia, Italy) for their critical reading of ... protein standard (lane 3), respectively (B) Spectral changes of hKAT-I following the addition of 10 mM glutamine into hKAT-I solution The concomitant decrease of the 422 nm peak and increase of the...

Ngày tải lên: 07/03/2014, 16:20

... Aceite de la Comunitat Valenciana Aceite de Madrid Andalucía Andalucía Andalucía Andalucía Andalucía Andalucía Andalucía Andalucía Andalucía Andalucía Andalucía Extremadura Extremadura Castilla-La ... Construction of Data Matrices and MLR Models 9.5 Prediction of OFA Concentration in VOOs Using FTIR and MLR 9.5.1 Description of FTIR Spectra and Construction of Data ... Construction of the Monolithic Columns and Optimization of the Separation Conditions 8.1.2 Characterization of the Phenolic Compound Profiles 8.1.3 Construction of Data Matrices and LDA Models...

Ngày tải lên: 07/03/2014, 21:20

... attachment of the protein to the cell surface of mouse lymphocytic leukemia cells [61], human umbilical vein endothelial cells, human promyelocytic leukemia cells, human ovarian carcinoma cells and ... activity and toxicity of the basic subunit, is a product of divergent evolution of the catalytically active and toxic PLA2 The salt bridge between Asp48 of the PLA2 molecule and Lys60 of the acidic ... facilitate identification of the key substrates of individual SVMPs and enable a better understanding of the molecular mechanism of action of P-III SVMPs Functional role of the non-enzymatic proteins...

Ngày tải lên: 14/03/2014, 22:20

... number of phosphate containing substrates, and it has a high specificity for nucleotide monophosphates and, in particular, UMP and GMP The structure of NagD has been determined and the occurrence of ... Fig Overproduction and purification of recombinant AraL-His6 (A) Analysis of the soluble (S) and insoluble (P) protein fraction (20 lg of total protein) of induced cultures of E coli Bl21(DE3) ... for the over-expression of the recombinant proteins The cells were grown in the presence and absence of the inducer isopropyl thio-b-d-galactoside (IPTG), and soluble and insoluble fractions...

Ngày tải lên: 14/03/2014, 23:20

... of Human Anatomy, Vol © 2003 Thieme All rights reserved Usage subject to terms and conditions of license Position of the Nervous System Introduction 4 5 A Position of the central nervous system ... recognized in the spinal cord and brain stem, knowledge of which will aid in understanding the organization of various parts of the brain The derivatives of basal and alar plates are difficult ... pekinensis Neanderthal Cro-Magnon B Endocranial casts of a gorilla and of fossil hominids C Endocranial casts of Homo sapiens, lateral view and basal view Kahle, Color Atlas of Human Anatomy,...

Ngày tải lên: 15/03/2014, 19:20

... spectroscopic and structural studies was tested by formation of the complex between RasGTPcNH2 and the Ras-binding domain of the Ras effector protein c-Raf-1 MATERIALS AND METHODS General description of ... methanol and repeated evaporation under reduced pressure, yield of the pure GTPcNH2 was 50–60% One gram of GTP triethylammonium salt was added to a stirred solution of 2.5 mL tributylamine and 1.2 ... )5.51 )5.30 – – 0.91 0.97 – – Preparation of NMR samples and NMR spectroscopy 31 P and 19F NMR spectra of free nucleotides were recorded in aqueous solution of 90%/10% H2O/D2O Typically the lyophilized...

Ngày tải lên: 18/03/2014, 01:20