identification and description of signalling molecules

... (e.g, Yu and Hatzivassiloglou (2003)) and classifying the polarity based solely on the subjective portions of the document (e.g., Pang and Lee (2004)) Motivated by the work of Koppel and Schler ... dataset Briefly, a positive review has a rating of ≥ 3.5 (out of 5) or ≥ (out of 4), whereas a negative review has a rating of ≤ (out of 5) or ≤ 1.5 (out of 4) 12 The curves are not shown due to space ... Hatzivassiloglou and K McKeown 1997 Predicting the semantic orientation of adjectives In Proc of the ACL/EACL, pages 174–181 618 M Hu and B Liu 2004 Mining and summarizing customer reviews In Proc of KDD,...

Ngày tải lên: 17/03/2014, 04:20

... dithiothreitol in a total volume of 50 lL Then, 40 lL of a reaction mixture composed of 200 lL of Hepes (1 M), pH 7.6, 40 lL of EDTA (0.2 M), 40 lL of NADPH (40 mgÆmL)1), and 500 lL of insulin (10 mgÆmL)1) ... with the addition of 10 lL of thioredoxin reductase, and incubation was continued for 20 at 37 °C The reaction was stopped by the addition of 0.5 mL of M guanidine-HCl and mM NBS2, and the absorbance ... ApTR (B) Molecular mass determination of ApTrx and ApTR Molecular masses of recombinant ApTrx and ApTR were determined by analysis of the elution files of standard proteins from a Sephacryl S-200...

Ngày tải lên: 23/03/2014, 21:20

... important aspects of interest for the outcome of PKU patients are quality of life and social outcome Quality of life (QoL) is a multidimensional concept reflecting the impact of disease and treatment ... patients and the general population Table 2: Comparison of mean QoL measured with the Profile of Quality of Life in the Chronically Ill (PLC) in PKU patients with the German norm; Mean and Standard ... did the data analysis and drafted the manuscript MS and UW conceived of the study and designed the questionnaires Both conducted the realisation and the analysis of the study and helped to draft...

Ngày tải lên: 18/06/2014, 22:20

... genomes Often the linear order of genes within the genomes of related viruses is maintained Thus, the spacing and orientation of specific genes can be predicted in the genomes of Page 15 of 24 ... review, the utility of CODEHOP-mediated PCR for the identification of novel viruses and the characterization of new viral genes and genomic regions is presented While the focus of this study was ... phylogenies and tracing of evolutionary pathways Furthermore, comparison of multiple representatives of homologous viral proteins can be of importance for understanding the protein structure and function...

Ngày tải lên: 18/06/2014, 22:20

... standardization and testing were carried out on separate sets of images, two for training and 12 for testing The number of samples used for standardization was 4, and 10 for the A, B, and C test functions, ... Haar wavelet and Daubechies orthogonal wavelets of length 4, 6, and However, since the images employed contain spurious structures at stochastic positions and of various sizes, the use of wavelets ... of rotation and all directions of deformation of the viral structures are expected to be present in the electron micrographs, these variables were not fixed Identification of points and local function...

Ngày tải lên: 20/06/2014, 01:20

... asphyxiation and the cells within the peritoneal cavity harvested by flushing the abdomen with 35 milliliters of sterile phosphate buffered saline (PBS, Grand Island Biological Co., Gibco BRL, Grand Island, ... et al.: Identification and characterization of a spontaneous ovarian carcinoma in Lewis rats Journal of Ovarian Research 2010 3:9 Page of Submit your next manuscript to BioMed Central and take ... 1.6 software, with the results normalized against mRNA transcripts for the housekeeping gene glyceraldehyde-3-phosphate dehydrogenase (GADPH) Results Description of proband Examination of a normal...

Ngày tải lên: 20/06/2014, 07:20

... mM EDTA, 1.5 M NaCl), in the presence of 0.1 mg of proteinase K and mg of lysozyme and incubated at 37°C for 30 Lysis was completed by adding ml of 20% SDS and incubated for h at 65°C with shaking ... Randomly selected transposon carrying protease negative mutants were sequenced and alignment of these sequences lead to the identification of the protease open reading frame (ORF) Analysis of ... 65 and 85% of the maximum activity at the temperature range of 35°C and 55°C respectively Thermal stability of the purified AS-protease was estimated at Figure Multiple sequence alignment of...

Ngày tải lên: 21/06/2014, 05:20

... ‘chemical genetic’ approach of identifying and studying the mechanism of action of small-molecule agonists (and antagonists), we hoped to uncover some of the complexities of the Hh-signaling system ... activation with a Hh-protein ligand has therapeutic value [49,50] On the basis of our current understanding of these models and the specific mechanism of action of the Hh agonists, we predict ... concentrations of Hh-Ag 1.3 (n = explants) Error bars represent standard deviations (c-n) Confocal images of representative explants cultured in the presence of different concentrations of the agonist and...

Ngày tải lên: 06/08/2014, 18:20

... buffer, 2.5 µl of 25 mM MgCl , µl of 2.5 mM deoxynucleoside triphosphate (dNTPs), 2.5 U of Taq-polymerase (Promega, USA), pmol of each primer, EEC and ECB (Genotech, Korea), and 200 ng of template ... conserved regions of the 16S rRNA gene fragment (116 bp) of both Ehrlichia spp and Anaplasma spp were used The composition of PCR mix, reaction conditions and the sequence of PCR probe were essentially ... extension at 72 C for 30 sec, and one cycle of extension at 72 C for For second round nested-PCR, pmol of each primer, HE1 and HE3 (Genotech, Korea) and µl of first PCR product as template DNA were...

Ngày tải lên: 07/08/2014, 18:21

... experiments and participated in the design of the study and analysis of the data FC participated in the design of the study and in the analysis of data and helped to draft the manuscript PM cloned and ... the analysis and interpretation of data and helped to draft the manuscript RR participated in the design of the study and in the revision of the manuscript EP participated in analysis of data GV ... recombinant protein and helped to interpret the data CA participated in the design and revision of the study and performed the statistical analysis RP participated in the design and revision of the study...

Ngày tải lên: 09/08/2014, 08:22

... quantitatively significant product of FLSs [4] and little is known about the processing and function of the c19orf10 molecule Materials and methods Cells, tissues, and synovial fluid Synovial tissue ... rupole time -of- flight mass spectrometer [10], and proteins were identified by single MS (peptide mass fingerprinting) using ProFound [11] and by tandem mass spectrometry (MS/ MS) using Tandem search ... Expression of c19orf10 in OA synovium (a) Intense staining of the synovial lining layer and perivascular regions of RA (OCT section) tissue.(b) Intense staining of the synovial lining layer and perivascular...

Ngày tải lên: 09/08/2014, 10:20

... cases of a gene that is in the process of being resurrected in the human lineage Identification and analysis of human unitary pseudogenes afford unique insights into the evolution and dynamics of ... approximately 75 MYA, represent, respectively, a loss and a gain of approximately 0.5% and 4% of the number of ancestral genes Despite aforementioned examples of gene losses under positive selection, this ... http://genomebiology.com/2010/11/3/R26 Page of 17 Figure Enrichment of Gene Ontology terms and Pfam domains in the human unitary pseudogene Enriched GO terms and their positions in the hierarchy of (a) biological process and (b)...

Ngày tải lên: 09/08/2014, 20:21

... help in the development of an accurate and complete structural annotation of the common bean genome, a valid transcriptome map, and the identification of the genetic basis of agriculturally important ... sequences will also help in the identification of transcription factors and small RNAs in common bean, understanding of gene families, and very importantly the development of molecular markers for common ... wilt [23] The breeding line BAT93 is one of the parents of the core common bean mapping populations, and therefore, understanding and identification of sequences expressed in the developing pod...

Ngày tải lên: 11/08/2014, 11:21

... amino-acid sequence of CaNDR1a (Figure 4), with residues S189 and G190 being strong ω-site candidates (with P-values of 2.48 × 10 -6 and 2.76 × 10 -5 , respectively) Furthermore, CaNDR1a and its Arabidopsis ... Maluf M, Fernandez D: Identification of coffee WRKY transcription factor genes and expression profiling in resistance responses to pathogens Tree Genet Genomes 2010, 6:767-781 19 Fernandez D, Santos ... obtained using version 2.0.10 of the Clustal X program [59] (b) Full length sequence alignment of CaNDR1a/b and the Arabidopsis protein NDR1, NHL16 and NHL38 Locations of the three NHL motifs within...

Ngày tải lên: 11/08/2014, 11:21

... phosphoproteomic analysis of woody plants Results Identification and characterization of the phosphoproteome of DTBs Total proteins were isolated from DTBs of poplar, and then digested with trypsin ... pSer and 10.6% pThr [22] and 88% pSer and 11% pThr [33] in Arabidopsis; and 86% pSer and 12.7% pThr in M truncatula [49] Table Characterization of identified phosphopeptides, phosphoproteins, and ... Discussion A series of differential expression profiling analyses of the induction, maintenance, and release of bud dormancy made it possible to identify a large set of dormancy-related candidate genes...

Ngày tải lên: 11/08/2014, 11:21

... contains eight distinct epitopes and most of these differ from the corresponding regions of other legume and tree-nut allergens [41] Identification of specific allergens and their IgE binding epitopes ... Vierucci A, Duranti M: One- and two-dimensional electrophoretic identification of IgE-binding polypeptides of Lupinus albus and other legume seeds Journal of Agricultural and Food Chemistry 2005, ... Casarotto and Elaine Smith for valuable technical assistance and Lars Kamphius and T.J Higgins for helpful comments on the manuscript This work was supported by CSIRO and the Centre of Food and Genomic...

Ngày tải lên: 11/08/2014, 11:21

... one of the most devastating diseases of wheat in China and worldwide and causing significant yield losses [28] High temperature, often combined with drought stress, causes yield loss and Page of ... siRNAs and 97 sequences could function in the form of long molecules involved in wheat resistance to powdery mildew infection and/ or heat stressed The collection of long npcRNAs offered candidates ... proteinase K and DNA was extracted and dissolved in 50 μl of ddH2O Additional file 6: Categories of siRNAs corresponding to SRP1 and SRP3 7S RNA variants and sequences of SRP1 and SRP2 corresponding siRNAs...

Ngày tải lên: 11/08/2014, 11:21

... Development of the flower shoot New Zealand Journal of Botany 1975, 13:473-483 Snelgar WP, Manson PJ: Determination of the time of flower evocation in kiwifruit vines New Zealand Journal of Crop and ... Page of 15 Figure Expression profiles of Actinidia flowering genes in normal and aberrant flowers Real-time RT-PCR analysis of the Actinidia flowering genes in the leaf and floral organs of A ... stages of the project, Andrew Gleave, Sakuntala Karunairetnam and Bhawana Nain Page 13 of 15 for cloning and sequencing support, Tim Holmes for photography, and Anne Gunson for critical reading of...

Ngày tải lên: 11/08/2014, 11:22

... Thr3, Thr11, and at several residues adjacent to them (Arg2, Lys4, Ser10, and Gly12) Using anti-H3T3ph antibodies, bands were detected in the case of normal histone H3 and S10A, T11A, and G12A mutants, ... Merged images of DNA (blue) and AtHaspin-tdTomato (red) are shown in color Scale bars: 10 μm aphidicolin (Figure 5A) This profile of AtHaspin expression was very similar to those of AtAURs and mitotic ... localization and dynamics of AtHaspin-FP reflect those of an endogenous Haspin protein in BY-2 cells This localization of AtHaspin-FP is consistent with that of phosphorylated H3T3 and H3T11 in...

Ngày tải lên: 11/08/2014, 11:22