identification in the atlantic bluefin tuna thunnus thynnus linnaeus

... histidine-tagged enzymes were generated in a similar way The coding sequences for six histidines were introduced by PCR either at the N-terminus or at the C-terminus To introduce the histidine-tag ... histidine-tag at the N-terminus, we introduced a coding sequence for MHHHHHHGG before the original start codon To introduce the histidine-tag at the C-terminus, we introduced the coding sequence ... vector in the same yeast strain When testing the two modified proteins in the crude extract of S cerevisiae, we observed that the N-terminally tagged enzyme did not exhibit any activity The C-terminally...

Ngày tải lên: 30/03/2014, 04:20

... with the exception of the N-terminus and the TM III–IV loop To examine the functions of TjZNT1 and TjZNT2, these transporters were tagged with hemagglutinin (HA) at their N-termini to verify their ... This indicates that the N-terminal region does not inactivate TjZNT2, but affects the ion selectivity of the protein Finally, tagging with HA at the N-terminus of TjZNT1 was found to alter the ... region contains no known metalbinding motifs, we speculate that it either interacts with other ion-selectivity regions in the protein, altering their FEBS Journal 278 (2011) 851–858 ª 2011 The Authors...

Ngày tải lên: 29/03/2014, 00:20

... the loops together with W140 constitute a nucleation center The indole ring [26] of W140 may be the main target for the interaction with both loop and loop Furthermore, from the protein folding ... DSC data show that the W140 in SNase is the amino acid responsible for the stability of the whole protein However, in comparison with the wild-type protein, the mutant W140A retains significant secondary ... a heating rate of °CỈmin)1 The melting point (Tm) of protein tested was directly obtained from the DSC curve The enthalpy change (DHcal) of each protein was calculated by integration of the curve...

Ngày tải lên: 20/02/2014, 01:20

... °C, washed three times in binding buffer, and the rpL32 binding proteins were eluted from the beads in m NaCl binding buffer Next, eluted fractions were equilibrated in binding buffer by ultrafiltration, ... fractions 22–25 obtained from the preparative EMSA stage; 2, the proteins bound by the lactofferin binding site in the last purification stage; 3, unbound proteins obtained from the last stage of ... with the rpL32 fragment ()24 +11)binding proteins detected by Southwestern assay (see above) Together, these findings indicate that p56 and A novel human (GCC)n-binding protein p68 are DNA-binding...

Ngày tải lên: 07/03/2014, 05:20

... near the flexible C terminus of SNase Changes in the fluorescence intensity of W140 reflect a change in the surrounding hydrophobic environment of the tryptophan and thus indicate a change in the ... 45% decrease in fluorescent intensity relative to the wild-type protein The fluorescence changes of W140 were in agreement with the thermodynamic results obtained from thermal unfolding (DHcal) ... see the following section and Table 1) These data indicate that the mutants are much less stable than the wild-type protein In the E129G mutant, the tryptophan at residue 140 may be exposed to the...

Ngày tải lên: 07/03/2014, 21:20

... exclusively in the mature part of the PORA protein and not in the transit peptide The position of the motif in the N-terminal region of the mature protein is in conflict with a function of the motif in ... possible N-termini for the PORB protein The N-terminal amino acid of the corresponding peptide signal with the most intense signal is homologous to the N-terminal amino acid of PORA (Fig 4) The two ... modifies the N-terminal amino groups of internal peptides, the hydrophobicity of the corresponding peptides is increased, leading to a delay in the retention time during chromatographic separation In...

Ngày tải lên: 30/03/2014, 02:20

... whereas the adaptive identification can be performed in a number of queries which is linear in the size of the graphs In [1], adaptive identification was studied in torii in the square lattice In this ... faulty vertex in the rectangle (rather than at most one faulty vertex in the identification problem) (3) In the search problem, all the queries are of the form: “is the faulty vertex in the rectangle ... (v)) Adaptive identification was defined in [9, Sec 1.2.7] and studied in [1], it consists in asking the queries one after the other, allowing one to choose the next query according to the answers...

Ngày tải lên: 08/08/2014, 14:23

... that by increasing the amount added to the protein the intensity of the 30 kDa band is progressively reduced The protease-containing fraction was then submitted to zymography using gelatine and ... increasing the bioavailability of IGFs by enhancing the proteolytic cleavage as well as for producing the C-terminal domain We have examined the amino acid sequences of the other protein family ... together with identical aliquots of the untreated proteins (Fig 9A) The degradation process was evaluated on the basis of the ratio between the integrated areas of the intact protein in the treated...

Ngày tải lên: 07/03/2014, 00:20

... that the failure of these seven mutations to abolish protein phosphorylation was a result of other protein kinases masking the PKA-catalysed part Indeed, protein kinases other than PKA, including ... by mutating seven S ⁄ Ts in GIRK1, including the structural determinants identified in the present study We suggest that also in this case the contribution of GIRK1 was masked by the indirect ... for PKA in vitro, after the in vivo treatment of the oocytes with a PKA inhibitor and also in untreated oocytes On the other hand, the in vitro phosphorylation signal was markedly diminished...

Ngày tải lên: 07/03/2014, 00:20

... experiments protein bound to the agarose This binding was confirmed by the observation that only a trace of the protein was left in the unbound fraction Pre-incubation of the recombinant protein with FP ... and the initial GS activity was maintained (116.5 ± 7.3% of the initial activity) The protective effect was abolished when the recombinant protein was heat-inactivated by autoclaving, suggesting ... FP–agarose, but the binding was not affected by preincubation of the protein with FP These results also indicate that Pf1-Cys-Prx can bind to FP at pH 7.4, which is the pH of the parasite cytosol The fact...

Ngày tải lên: 07/03/2014, 17:20

... N-terminal modifications have not been identified in the rice proteasome Processing of proteins is important for their function In the 20S proteasome, processing during assembly produces the active ... 1A) These results indicated that each gene has a single copy in the genome As OsRpt6 has an EcoRV site in the cDNA sequence determined, the probe of OsRpt6 must hybridize to two bands of the ... analyses In this analysis, the products of all the rice RP genes obtained from the EST library were found in the RP complex purified from rice bran (Tables and 3) Two proteins contained in bands...

Ngày tải lên: 08/03/2014, 16:20

... as the dialogue act performed by the speaker in that situation, the class variable is the addressee of the dialogue act (ADD) Since there are only a few instances of subgroup addressing in the ... results combining MATYPE with the initial feature set The results indicate that adding meeting context information to the initial feature set improves slightly, but not significantly, the classifiers’ ... Additionally, the available annotations of meeting actions for the M4 meetings3 were converted into the corpus format and included in the collection The dialogue act tag set employed for the corpus...

Ngày tải lên: 08/03/2014, 21:20

... result also provides indirect evidence of correct pairing of the interchain disulfide bridges in the recombinant sample [11] The kinetics of the N-terminal arms swapping in the P19A-BSRNase is ... determinants for this process are located in regions different from the hinge loop 16–22 In the search for the region primarily involved in the swapping, we focused our attention on the interface ... (Fig 5B) Interestingly, the amount of the MxM form at the plateau as evaluated by integrating the peaks obtained on gel filtration is 70%, i.e in complete agreement with that obtained for the native...

Ngày tải lên: 16/03/2014, 23:20

... - opinion word pairs, negatively affecting the precision The algorithm cannot distinguish them from opinions expressing the stance of the author Overall, the recall of the baseline is rather ... Christopher Manning 2005 Incorporating non-local information into information extraction systems by gibbs sampling In Proceedings of the 43rd Annual Meeting of the Association for Computational Linguistics, ... Lecture Notes In Computer Science, pages 344–355 Springer, Shanghai, China Minqing Hu and Bing Liu 2004 Mining and summarizing customer reviews In Proceedings of the 10th ACM SIGKDD International...

Ngày tải lên: 17/03/2014, 00:20

... non–existent in the PTB, while it is the 16th most frequent dependency in the ATB, and that the performance of the parser they worked with (the Bikel implementation (Bikel, 2004) of the Collins parser) ... Some Results In the usual manner, we divided the data into training, development test, and test sections according to an 80/10/10 division As the work in this paper is preliminary, the test section ... modification, meaning the streets of the city of Long Beach in the state of California al., 1995) forces multiple PP modifiers of the same NP to be at the same level, disallowing the structure (B) in favor...

Ngày tải lên: 17/03/2014, 02:20

... Substitution of the residues involved in these interactions would therefore be expected to impair the formation or stabilization of the complex, or both, thereby affecting the toxicity of the protein This ... protein This is probably the case for changes in the residues involved in the interaction between the two subunits: Asn1Ser, Phe3Leu in the PLA2 subunit and Gln34His in the inhibitor subunit (Fig ... clones having the 126 bp deletion as in V a aspis, V a zinnikeri, and the remaining four clones having an intron D similar to that of the V am ammodytes ammodytin I1 gene All PLA2 genes contained...

Ngày tải lên: 17/03/2014, 03:20

... heparin-binding residues are present within the aminoterminal heparin-binding domain (ATD) of PKR Thus, in contrast to the dsRNA-binding domain of PKR, its heparin-binding domains are located in the ... carboxy-terminal half of the molecule The aminoterminal heparin-binding domain (ATD) overlaps with the ATP-binding catalytic subdomain II and the carboxy-terminal heparin-binding domain (CTD) is ... its minor role in heparin binding (Fig 2) However, when combined with the DM, it resulted in 86% loss of heparin binding As the dsRNA–binding domain does not overlap with PKR’s heparin-binding...

Ngày tải lên: 23/03/2014, 13:20

... emotional engagement in the task, they were first asked to rate their personal attitude to the two airlines The ratings in all three cases were on the extreme ends of the point scale, with very ... (branchingFactor) and its siblings: Spc = (depth+ln(siblings)−ln(branchingF actor)) N ormalizingF actor (1) The three metrics are further specialised according to the following two boolean flags: InText: ... when evaluating news where the text is intended to appear impartial The attitude of the evaluator can be all important: their attitude to the individuals or organisations in the text, their professional...

Ngày tải lên: 23/03/2014, 18:20

... of the soluble and insoluble fractions was performed (Fig 1B) The subunits of the diol dehydratase, mainly the medium subunit, were present in the insoluble fraction suggesting that the recombinant ... of the diol dehydratase were cloned in the pQE30 expression vector system The synthesis of the three subunits of the protein was controlled by SDS/PAGE (Fig 1A) Despite the high level of synthesis ... [24]), or by reducing the induction period or induction temperature (data not shown) To determine if the three subunits of the enzyme were expressed in a soluble form or in inclusion bodies,...

Ngày tải lên: 23/03/2014, 21:20

... buried in the interior of the protein with K401 actually appearing on the opposite face of the protein from the majority of residues in the F11 epitope [30] Assuming that the F11 antibody is binding ... are located in the C1 domain In particular, these results implicate this region in the binding of hTF to TFR Results TFR binding studies The results of a study designed to determine the ability ... epitope of the F11 antibody is in the C-lobe of hTF, specifically within residues 365–401 of the C1 domain Furthermore, binding of the F11 antibody to hTF inhibits binding to TFR (Fig 1) The residues...

Ngày tải lên: 30/03/2014, 11:20