id1 smad6 and snail expression in ovarian cancer cell lines
báo cáo khoa học: "ShRNA-mediated gene silencing of MTA1 influenced on protein expression of ER alpha, MMP-9, CyclinD1 and invasiveness, proliferation in breast cancer cell lines MDA-MB-231 and MCF-7 in vitro" pptx
Ngày tải lên :
10/08/2014, 10:21
... transfected into the breast cancer cell lines MDA-MB-231 and MCF-7 Its effect on protein expression of estrogen recepter alpha(ER a ), matrix metalloproteinase 9(MMP-9), cyclinD1, and on cancer cells invasion, ... ShRNA-mediated gene silencing of MTA1 influenced on protein expression of ER alpha, MMP-9, CyclinD1 and invasiveness, proliferation in breast cancer cell lines MDA-MB-231 and MCF7 in vitro Journal of ... Ding An Corp in China for sequencing Methods Cell lines and culture Transfection with shRNA/MTA1 expression vector The human breast cancer cell lines MDA-MB-231 and MCF-7 were kindly supplied by...
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báo cáo hóa học:" ST6Gal-I expression in ovarian cancer cells promotes an invasive phenotype by altering integrin glycosylation and function" pptx
Ngày tải lên :
20/06/2014, 07:20
... sialylation of β1 integrins in three ovarian carcinoma Figure cell lines2 α2–6 sialylation of β1 integrins in three ovarian carcinoma cell lines. A, Lysates from PA1, OV4, and SKOV3 cells were incubated ... three ovarian carcinoma cell lines for ST6Gal-I Screen of three ovarian carcinoma cell lines for ST6Gal-I expression PA1, OV4, and SKOV3 cells were grown in culture, lysed, resolved under reducing ... caused increased binding to collagen I and laminin, and increased cell motility [12] This change in cell behavior was shown to be a consequence of the hypersialylation of the β1 integrin Though incompletely...
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báo cáo hóa học:" Aberrant STYK1 expression in ovarian cancer tissues and cell lines" pdf
Ngày tải lên :
20/06/2014, 07:20
... 2receptors lines express STYK1 and variably express Ovarian tumor cell lines express STYK1 and variably express estrogen receptors HS832 is a benign ovarian cell line and the remaining cell lines were ... receptors and STYK1 in ovarian cancer cell lines We detected ER- RNA expression in ovarian cancer cell lines SKOV3, CaOv3, and OvCar3 (Fig 2A) Cell lines HS832, OvCar5, OvCar8 and IGROV had no detectable ... OvCar8, and IGROV1 GPR30 and STYK1 protein was detected at varying levels in each cell line (Fig 2B) Estradiol and GPR30-specific G-1 induce STYK1 RNA but not protein expression in ovarian cancer cell...
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báo cáo hóa học:" Curcumin induces chemo/radio-sensitization in ovarian cancer cells and curcumin nanoparticles inhibit ovarian cancer cell growth" pot
Ngày tải lên :
20/06/2014, 07:20
... mechanisms involved in curcumin mediated chemo/radio-sensitization in ovarian cancer cells Materials and methods Cell culture and drugs A2780 and A2780CP (resistant to cisplatin) paired cells [18] ... Curcumin treatment reduces overall β-catenin and c-Myc protein levels A2780CP cell lines were treated as in (A), protein lysates were collected and analyzed by immunoblotting for β-catenin, c-Myc and ... article as: Yallapu et al., Curcumin induces chemo/radio-sensitization in ovarian cancer cells and curcumin nanoparticles inhibit ovarian cancer cell growth Journal of Ovarian Research 2010, 3:11 Page...
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báo cáo khoa học: " Platinum resistance in breast and ovarian cancer cell lines Niels Eckstein" pptx
Ngày tải lên :
10/08/2014, 10:21
... Oxaliplatin (Eloxatin®) (Figure 1) are first-line anti -cancer drugs in a broad variety of malignancies, for instance: ovarian cancer, testicular cancer and non small cell lung cancer Cisplatin is inactive ... platinum aquocomplexes by Sulphur-containing peptides or proteins, increased DNA repair, and alterations in apoptotic signaling pathways (reviewed in [5]) Cisplatin and Carboplatin resistant cells ... hepatocellular carcinoma cells with elevated thioredoxin levels are resistant to Cisplatin, but not to the antracyclin Doxorubicin [39] However, bladderand prostate cancer cell lines with TRX overexpression...
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MECHANISM OF TISSUE TRANSGLUTAMINASE UPREGULATION AND ITS ROLE IN OVARIAN CANCER METASTASIS
Ngày tải lên :
24/08/2014, 10:58
... metastasis in vivo The N-terminal fibronectin (FN) binding domain of TG2 (tTG 1-140), lacking both enzymatic and GTPase function, induced EMT in OC cells, suggesting the interaction with FN involved in ... induces EMT in OC cells 102 3.2.5 TGF-β1 induces an ovarian cancer stem cell phenotype 107 3.2.6 TG2 is upregulated in ovarian cancer stem cells 110 3.2.7 TG2 induces an ovarian cancer stem cell ... hinge region, which is between the N-terminal ECM binding domain and the C-terminal LAP binding domain This can be achieved by many proteinases in the serine proteinase family, such as plasmin,...
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Báo cáo khoa học: Wild-type p53 enhances annexin IV gene expression in ovarian clear cell adenocarcinoma docx
Ngày tải lên :
22/03/2014, 16:20
... higher in CCA than non-CCA cell lines and tissues [13] We confirmed this finding by western blotting and real-time RT-PCR analyses using cell lines originating from CCA, OVTOKO and OVISE cultured cell ... gene in OVTOKO and OVISE cells but not in MCAS cells, indicating that endogenous p53 protein directly binds to the ANX4 gene in CCA cells To verify the involvement of p53 in the CCA-specific expression ... cell lines, those of p53 target genes, p21 and murine double minute (MDM2), as well as ANX4, were significantly higher in CCA cell lines than non-CCA-type EOC cell lines (Fig 5A) In addition, in...
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Báo cáo hóa học: " Prognostic significance of Oct4 and Sox2 expression in hypopharyngeal squamous cell carcinoma''''" doc
Ngày tải lên :
18/06/2014, 16:20
... localized in the nucleus of tumor cells (A) High Oct4 expression in tumor cells, (B) low Oct4 expression in tumor cells, (C) high Sox2 expression in tumor cells, and (D) low Sox2 expression in tumor cells ... and Sox2 expression in hypopharyngeal squamous cell carcinoma Brown grains represent a positive signal (3, 3-diaminobenzidine staining) The positive expression site of Oct4 and Sox2 was mainly localized ... localized in the nucleus, were observed in the cancer cells of tumor tissues (Fig 1) The distribution of immunostaining Figure Expression of Oct4 and Sox2 Immunohistochemical staining for Oct4 and...
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Báo cáo sinh học: " High Chromosome Number in hematological cancer cell lines is a Negative Predictor of Response to the inhibition of Aurora B and C by GSK1070916" potx
Ngày tải lên :
18/06/2014, 22:20
... GSK1070916 The cell line response data was compared to the mutation patterns in the cell lines, gene expression patterns and the karyotypes of the cell lines High chromosome number in the cell lines was ... hematological cell lines using cell cycle analysis and cell death measures to determine sensitivity and resistance Cell lines that are early and moderate responders by cell cycle analysis with a Ymin/T0 ... lymphoma cell lines, of the cell lines were resistant to GSK1070916 and contained low chromosome number in the primary population of cells However, when in reviewing the ploidy content in the cell...
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o cáo hóa học:" High Chromosome Number in hematological cancer cell lines is a Negative Predictor of Response to the inhibition of Aurora B and C by GSK1070916" ppt
Ngày tải lên :
20/06/2014, 04:20
... GSK1070916 The cell line response data was compared to the mutation patterns in the cell lines, gene expression patterns and the karyotypes of the cell lines High chromosome number in the cell lines was ... hematological cell lines using cell cycle analysis and cell death measures to determine sensitivity and resistance Cell lines that are early and moderate responders by cell cycle analysis with a Ymin/T0 ... lymphoma cell lines, of the cell lines were resistant to GSK1070916 and contained low chromosome number in the primary population of cells However, when in reviewing the ploidy content in the cell...
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báo cáo khoa học: "Enhancive effects of Lewis y antigen on CD44-mediated adhesion and spreading of human ovarian cancer cell line RMG-I" pps
Ngày tải lên :
10/08/2014, 10:21
... S: CD44 stimulation induces integrin-mediated adhesion of colon cancer cell lines to endothelial cells by up-regulation of integrins and c-Met and activation of integrins J Cancer Res 1999, 59:4427-4434 ... CD44mediated adhesion and spreading of ovarian cancer cells Inhibiting the expression of CD44 or blocking its Figure The mRNA expression of CD44 and a1, 2-FT in RMG-I and RMG-I-H cells were tested ... breast cancer[ 28] It is also confirmed that the binding of CD44 to HA induces c-Src kinase activation, and is involved in the metastasis of ovarian cancer cells by activating the c-Src kinase...
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Báo cáo khoa học: The in vitro effects of CRE-decoy oligonucleotides in combination with conventional chemotherapy in colorectal cancer cell lines potx
Ngày tải lên :
07/03/2014, 15:20
... fetal bovine serum HCT116 and GEO cell lines were both wild-type p53, and SW620 lines were mutant p53 No antibiotics were used in our experiments, and all cell lines were incubated in a humidified ... Therefore, we assessed p21waf1 levels in GEO and HCT116 cell lines, and showed that combination therapy induced p21waf1 in the GEO cell line only (the cell line in which synergistic effects were ... protein expression following culture with CDO and 5-FU together were similar in GEO and HCT116 cells (A) The only protein that was expressed differently in the cell lines was p21waf1, which was increased...
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báo cáo hóa học:" MicroRNA signature of cis-platin resistant vs. cisplatin sensitive ovarian cancer cell lines" pot
Ngày tải lên :
20/06/2014, 08:20
... determine any potential miRNA that could be linked to cis-platin resistance by identifying miRNA differences in cis-platin resistant and cis-platin sensitive cell lines Page of 11 Methods Cell lines ... achieve cell death as compared to A2780 cells Cell lines were cultured in RPMI 1640 supplemented with 10% fetal bovine serum and 1% antibiotics (Invitrogen, Carlsbad, CA) and maintained in a humidified ... 37°C and 5% CO The cell lines were sub-cultured on routinely basis every 3-4 days Cell viability assays A2780 and A2780/CP70 cell lines were cultured to test the responsiveness of each cell line...
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báo cáo khoa học: "Promising cytotoxic activity profile of fermented wheat germ extract (Avemar®) in human cancer cell lines" pot
Ngày tải lên :
10/08/2014, 10:21
... Overall, mainly significant synergy was observed for the combinations of FWGE and 5-FU (6 out of cell lines) and to a lesser extend for irinotecan and oxaliplatin (2 out of cell lines) Drug interaction ... activity of FWGE was found on neuroblastoma and ovarian cancer cell lines It’s interesting to note that the IC50-values of the human CRC cell lines included in this screen range close to the average ... neuroblastoma and ovarian cancer cell lines Of particular interest for further clinical development is the relative homogeneous sensitivity of the eight colon cancer cell lines employed in this study...
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báo cáo khoa học: "Improved retroviral suicide gene transfer in colon cancer cell lines after cell synchronization with methotrexate" docx
Ngày tải lên :
10/08/2014, 10:21
... effective in synchronizing DHDK12 cells in S phase than ara-C or aphidicolin Consequently, the efficacy of MTX in synchronizing cells in S phase was then tested in the HT29 cell line In HT29 cell line, ... France) cell lines Cell lines were cultured in DMEM medium containing 10% calf serum/penicillin (50 units/ ml)/streptomycin (50 μg/ml) at 37°C in 5% CO We used retroviral vectors carrying Escherichia-coli ... colon cancer cell lines used in this study, further investigations in more colon cancer cell lines are needed to draw definitive conclusion on the improvement of retroviral gene transfer after cell...
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báo cáo hóa học:" MUC4 stabilizes HER2 expression and maintains the cancer stem cell population in ovarian cancer cells" ppt
Ngày tải lên :
20/06/2014, 07:20
... signaling [7,8] Our recent finding showed that an overexpression of MUC4 increases the expression of HER2 in ovarian cancer cells [8] and stabilizes the HER2 oncoprotein in pancreatic cancer cells ... In the present study, we analyzed the expression of the HER2 protein in MUC4transfected ovarian cancer cells The exogenous MUC4 expression in ovarian cancer cells showed an increase in HER2 expression ... SKOV3 cells (Figure 1B) Overexpression of MUC4 stabilizes HER2 in ovarian cancer cells In our previous studies we have shown that MUC4 interacts with HER2 in ovarian cancer and pancreatic cancer cells...
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báo cáo khoa học: " Expression of Ets-1, Ang-2 and maspin in ovarian cancer and their role in tumor angiogenesis" pps
Ngày tải lên :
10/08/2014, 10:21
... been fully investigated in ovarian cancer In the present study, we examined the relationship between the expression of Ets-1 and its targets Ang-2 and maspin in ovarian cancer and their clinical ... staining for Ets-1, Ang-2 and Maspin in ovarian tumor tissues A: Ets-1 expression in ovarian moderately and poorly differentiated serous adenocarcinoma; B: Ets-1 expression in ovarian borderline ... borderline mucinous cystadenoma; C: Ang-2 expression in left ovarian serous papillary cystadenocarcinoma; D: Ang-2 expression in ovarian borderline mucinous cystadenoma; E: Maspin expression in mucinous...
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báo cáo hóa học:" BMP-2 signaling in ovarian cancer and its association with poor prognosis" pot
Ngày tải lên :
20/06/2014, 07:20
... of Ovarian Research 2009, 2:4 Results BMP-2 activates SMAD 1/5/8 and Erk MAPKs in ovarian cancer cell lines To investigate the role of BMP-2 in ovarian cancer cells we selected three cell lines, ... pancreatic carcinoma cells and prostate cancer cells in absence of androgen [18,19] On the other hand, BMP-2 clearly inhibits the growth of tumor cells of many origins including cancers arising from ... tested (Figure 2E) BMP-2 increases Id1, Smad6 and Snail expression in ovarian cancer cell lines We next examined whether the signaling pathways activated by BMP-2 led to the expression of known target...
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báo cáo hóa học:" Positron emission tomography in ovarian cancer: 18F-deoxy-glucose and 16α-18F-fluoro-17β-estradiol PET" docx
Ngày tải lên :
20/06/2014, 07:20
... accuracy in diagnosis, staging, and management decisions in ovarian cancer In this review article, the role of FDG-PET and FDG-PET/ CT in the diagnosis, staging, and management of ovarian cancer ... gynecologic cancer and in determining the efficacy of hormonal therapy [18,19], as a future PET method for evaluating ovarian cancer Imaging protocol for ovarian tumors FDG is excreted through the urinary ... patients with early mucinous adenocarcinoma and borderline mucinous adenocarcinoma Rieber et al reported that early carcinomas, mucinous adenocarcinomas, and particularly borderline tumors, present...
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báo cáo hóa học:" Mucins in ovarian cancer diagnosis and therapy" ppt
Ngày tải lên :
20/06/2014, 07:20
... different mucins in ovarian tumors and their potential role in ovarian cancer diagnosis and treatment Mucins Being that 90% of ovarian cancers are of epithelial origin, mucins may be attractive candidates ... of mucins in ovarian cancer and show that mucin expression may alter the cellular characteristics of ovarian cancer cells and also imply a significant role of mucins in the pathogenesis of ovarian ... nonmalignant ovarian epithelial cell lines also express MUC1 (a membrane associated mucin) and MUC5AC (a secreted mucin) [47] Mucin Expression in Ovarian Tumors The expression of mucin genes by ovarian...
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