i and in vitro values fo

... containing no Cu (left) The difference between the amount released at 48 h and that contained in the original loading solution determined Cu loading, resulting in values ranging between 12 and ... nanoparticles MC carried out particle synthesis, and metal loading RP conceived of the study, and participated in its design and coordination, carried out in vitro toxicity studies, SEM and EDX ... the antiproliferative effect and intracellular glutathione depletion induced by Casiopeína IIgly and cisplatin in murine melanoma B16 cells Toxicol in Vitro 2011, 25:868-873 36 Alemón-Medina R,...

Ngày tải lên: 21/06/2014, 02:20

... which in combination with low concentration of auxin, in rooting medium, was essential in stimulating high rooting percentage with high quality of roots, resulting in fast growing plantlets during ... catabolism, which is well known to increase peroxidase activity reducing IAA in the induction phase [23] Putrescine not only induces an increase in rooting percentage itself, but it requires a ... putrescine treatments on in vitro rooting and on endogenous polyamine changes in difficult-to-root woody species, Sci Hortic 53 (1993) 63–72 [22] Rugini E., Involvement of polyamines in auxin and...

Ngày tải lên: 07/08/2014, 16:20

... actin (F-actin) was not markedly different in either condition (original magnification ×200) collagen type I and II, whether in monolayer or alginate cultures (Figure 4b) Staining for F-actin was ... with streptavidin-FITC (fluorescein isothiocyanate) (Vecta Labs Ltd.) and counterstained with DAPI (4'-6-diamidino-2-phenylindole) Immunolocalisation was also performed using isotype-matched irrelevant ... manuscript Available online http://arthritis-research.com/content/10/2/R46 Additional files The following Additional files are available online: Additional file 'Live/dead' staining of intervertebral...

Ngày tải lên: 09/08/2014, 10:23

... competing interests Authors' contributions PNM participated in the study design and experimental work SCR participated in the study design, participated in its coordination and drafted the manuscript ... embedded in paraffin Histological sections of μm thickness were stained with hematoxylin and eosin The specimens were evaluated Page of (page number not for citation purposes) Acta Veterinaria Scandinavica ... structure will depend on the clinical application [2] The multipotent mesenchymal stem cells are becoming a subject of increasing interest because of their potential in tissue engineering applications...

Ngày tải lên: 12/08/2014, 18:22

... because values of ki are not available for certain tissues For scaling of the brain and the epithelial tissues of the skin and gastrointestinal tract, the midpoint of the values of bi is 0.71 The midpoint ... midpoint of the values of ki is -0.14, the midpoint of the values of scaling exponents for the SMR of tissues reviewed in the introduction Therefore, the scaling of the BMR contribution of this ... As explained in the PVFCP model, pressure in capillaries necessarily falls as the rate of blood flow in a tissue decreases (assuming that the level of constriction in veins draining the tissue...

Ngày tải lên: 13/08/2014, 23:20

... region of amino acid sequence information is shown in Figure 5.2 To reduce manipulations and avoid any contamination problems both the initial and nested PCR reactions can be performed in a single ... using in vitro assays Furthermore, rapid in vitro protein–protein interaction studies could be performed using this technique where not only full-length version of proteins could be expressed and ... Analysis, sequencing and in vitro expression of PCR products 103 H-labeled PCR product Heat denature Anneal with biotinylated probe B Streptavidin binding B Scintillation generates light emission...

Ngày tải lên: 25/10/2013, 22:20

... transformed with mSR-BI) were maintained in medium containing 0.5 mgÆmL)1 G-418 Both cell lines were kindly provided by Dr M Krieger (MIT, Cambridge, MA, USA) Binding studies Binding studies of human ... choriocarcinoma cells (24 h in DMEM containing 10% FBS, 1% glutamine and 1% penicillin/streptomycin) and CHO cells (HAM’s F12 medium containing 5% (v/v) FBS, mM glutamine, 50 UÆmL)1 penicillin and ... cm2 they were rinsed once with NaCl/Pi and infected with recombinant adenoviruses (MOI ¼ 30 pfuÆmL)1) in mL of infection media (DMEM medium containing 2% FBS, 50 UÆmL)1 penicillin, and 50 lgÆmL)1...

Ngày tải lên: 20/02/2014, 23:20

... nanowires after flooding the chamber with diluted silane and explained the absence of nanowires by considering the surface tension of indium and the low solubility of silicon in indium Iacopi et al [18] ... droplet formation between indium and gold, we will consider in the following the effects of different diffusion coefficients of gold and indium on silicon, the solubility of substrate atoms in the ... decomposition of a silicon oxide layer by indium were found From the phase diagrams In Si and Au–Si (Fig 4), it can be seen that the solubilities of silicon in gold and indium at our growth temperatures...

Ngày tải lên: 16/03/2014, 15:17

... sequences, Oligo I and Oligo III but not with Oligo II (data for Oligos I and II are shown in Fig 8) The specificity of the binding was further confirmed by competition assays where excess concentrations ... were included in the reaction mix Addition of ATP abolished the DNA–protein complex formation with Oligo I The binding with Oligo I was also studied following phosphorylation/dephosphorylation ... (1993) A calcium/calmodulin-binding serine/threonine protein kinase homologs to the mammalian type II calcium/calmodulindependent protein kinase is expressed in plant cells Plant Physiol 101, 1381–1384...

Ngày tải lên: 18/03/2014, 01:20

... )%57( III puorg dna )%77( II puorg ni rewol )50.0 < p( yltnacifingis saw etar noisuf eht tub ,)%97( I puorg ni noitroporp eht naht rehgih )50.0 < p( yltnacifingis erew )%69( III puorg dna )%59( II ... metsys noitarutam denifed a ,noitidda nI soyrbme gip TNCS fo tnempoleved dna noitarutam etycoo rof laicifeneb saw h 24 rof setycoo fo MVI taht detacidni hcihw ,snoitidnoc noitarutam fo snoitacifidom ... gnitomorp-noitarutam suoirav fo noitca eht fo ytilareneG ni ortiv ni ortiv oviv ni ortiv ni ortiv ni H inatanaK ,H odnoK ,R amayiruK ,T otomihsiK 12 971-371 ,7 ,9991 etogyZ dega dna derutam setycoo enicrop...

Ngày tải lên: 07/08/2014, 20:23

... Asn341-Phe342 site coincides with the initiation of collagen damage in murine antigeninduced arthritis: a pivotal role for stromelysin in matrix metalloproteinase activity Arthritis Rheum 1999, ... Interleukin1beta-stimulated invasion of articular cartilage by rheumatoid synovial fibroblasts is inhibited by antibodies to specific integrin receptors and by collagenase inhibitors Arthritis Rheum ... performed some of the experiments and participated in writing the manuscript SW established the initial form of the in vitro destruction model and described it in his diploma thesis RWK contributed...

Ngày tải lên: 09/08/2014, 01:22

... of antiinflammatory cytokines such as transforming growth factorbeta whilst inhibiting the production of proinflammatory cytokines, including tumour necrosis factor-alpha [11] ated human discs ... cells within the intervertebral disc is reported to be extensive [35] and increasingly common after the age of 11 in Whilst apoptosis of cells within the intervertebral disc has been investigated ... (Sigma-Aldrich, Gillingham, Dorset, UK) containing 1.67 units per millilitre DNase (Sigma-Aldrich) The cells obtained after digestion were washed using Dulbecco's modified Eagle's medium (DMEM)/F-12 (Invitrogen...

Ngày tải lên: 09/08/2014, 10:23

... of VM in GBC by using immunohistochemistry staining, TEM and micro-MRA technology in vitro and in vivo, respectively All authors have read and approved the final manuscript 19 Competing interests ... After being rinsed with PBS again, the sections were incubated with goat antimouse Envision Kit (Genetech, USA) for 40 at 37°C followed by incubation with 3, 3-diaminobenzidine (DAB) chromogen for ... two different tumor cell lines, including GBC-SD and SGC-996, can exhibit significant different invasive ability and possess discrepancy of VM channels formation In this study, we show evidence...

Ngày tải lên: 10/08/2014, 10:21

... inflammatory stimuli comprises pro-inflammatory cytokines and chemokines, including the T cell inhibitory cytokine IL-10, the Th-1 promoting cytokine IL-12, as well as TNF-α and IL-8 [1923] In ... cells), with decreased CFSE staining intensity resulting from dilution during cell division (viz., the fluorescence intensity of membrane staining halves with each cell division) Background proliferation ... addition, cyclooxygenase-2 (COX-2), an enzyme responsible for converting arachidonic acid to prostaglandin-E2 (PGE-2), is induced in response to inflammatory stimuli and results in the production...

Ngày tải lên: 11/08/2014, 10:23

... High rates of infection in HIV-1-infected individuals in Africa have been suggested to be related to immune hyperactivation driven by environmental factors, including high exposure to infectious ... activation and reduced in vitro susceptibility to HIV-1 infection in exposed seronegative individuals than in low-risk Central African blood donors Results Characteristics of study population Forty-five ... susceptibility to in vitro infection using two HIV-1 strains with different tropisms, HIV-1 NL-4.3 (X4) and HIV-1 BaL (R5) In order to evaluate the impact of the baseline activation of PBMC on their...

Ngày tải lên: 13/08/2014, 09:20

... in hair follicles is optimal for follicular morphogenesis and may greatly influence the intensity of EMIs 26 Therefore, the idea that artificially assembling cell composites mimicking hair follicle ... methods including flow lithography and 3D bioprinting are also involved in fabricating tissue engineering scaffolds photolithography-based microfluidic technique 99 Flow lithography is a that permits ... disclosed 1.2.3 3D microstructure fabrication in tissue engineering Tissue engineering aims to regenerate biological tissues and organs by using cells within an artificially-created supporting...

Ngày tải lên: 09/09/2015, 11:13

... non-generic and nontrivially coupled to the environment Since it is an intrinsically interdisciplinary research, it involves expertise and perspectives from different disciplines such as engineering, ... both scientific and commercial communities The main advantage of using modeling approaches is the reduction in time and cost required for conducting experimental investigations by pinpointing the ... Linear Programming mAb Monoclonal Antibody MDS Multidimensional Scaling MFA Metabolic Flux Analysis MILP Mixed Integer Linear Programming MINLP Mixed Integer Nonlinear Programming MOMA Minimization...

Ngày tải lên: 11/09/2015, 21:27

... used in the following in vivo and in vitro studies 3.3 In vitro Study To mimic the functional response of Th17 and other Th cell subpopulations to HDM allergens, an in vitro allergen stimulation ... activities in PBMCs isolated from AR patients, which was concordant to the in vivo situation Th17 cells mediate airway inflammation by producing its unique cytokine IL17 to induce those proinflammatory ... function of IL10+ cells may be impaired in severe inflammation, while it can be activated in mild inflammatory or healthy status, showing a similar pattern as those found in the in vivo situation...

Ngày tải lên: 23/09/2015, 14:47

... Cephalosporin C 5-Fluorouracil Cisplatin Ifosfamide Paraquat Arsenic(III) oxide Bismuth(III) oxide Cadmium(II) chloride Copper(II) chloride Germanium(IV) oxide Gold (I) chloride Lead acetate Potassium dichromate ... development of in vitro models.  19 1.4 Role of inflammation in drug-induced nephrotoxicity in humans In the development of in vitro models for the prediction of drug-induced nephrotoxicity, it is important ... nephrotoxicity Aminoglycoside antibiotics, such as gentamicin, tobramycin and amikacin, are effective drugs in clinical use to treat sepsis, a systemic response to infections Aminoglycosides are...

Ngày tải lên: 30/09/2015, 06:22

... amounts eliminated in the urine[44, 78, 83] Following oral administration of tamoxifen, elimination is biphasic and is dependent on the cumulative dose The terminal elimination half-life (t1/2β) ... In Vitro Studies 5.1 PROTEIN BINDING STUDY 5.1.1 Introduction The binding of drugs to plasma and tissue proteins is a major determinant of drug distribution throughout the body Protein binding ... phases where food may possibly interact with a coadministered drug are: 1) before and during gastrointestinal absorption; 2) during distribution; 3) during metabolism; 4) during elimination[147]...

Ngày tải lên: 09/10/2015, 11:24