hybridization washes probe detection and signal development

... Design of oligonucleotide probes for development of reverse line probe hybridization assay Twenty-five oligonucleotide probes were designed within the region of the COG1F-G1NVR and COG2F-G2NVR PCR ... and G2NVR (Table 1) Eight oligonucleotide probes were designed for the detection of GI NoV and 17 probes were designed for the detection of GII NoV The probes were designed based on the criteria ... 60°C and a minimum of mismatches between the probe and the other genotypes All the probes were submitted to BLASTn program National Centre for Biotechnology Information to verify specificity A probe...

Ngày tải lên: 18/06/2014, 18:20

... Design of oligonucleotide probes for development of reverse line probe hybridization assay Twenty-five oligonucleotide probes were designed within the region of the COG1F-G1NVR and COG2F-G2NVR PCR ... and G2NVR (Table 1) Eight oligonucleotide probes were designed for the detection of GI NoV and 17 probes were designed for the detection of GII NoV The probes were designed based on the criteria ... 60°C and a minimum of mismatches between the probe and the other genotypes All the probes were submitted to BLASTn program National Centre for Biotechnology Information to verify specificity A probe...

Ngày tải lên: 20/06/2014, 01:20

... Temporal Signals in Gaussian Noise Signal Detection: Known Gains • Signal Detection: Unknown Gains • Signal Detection: Random Gains • Signal Detection: Single Signal 13.6 Spatio-Temporal Signals Detection: ... 13 Signal Detection and Classification 13.1 Introduction 13.2 Signal Detection The ROC Curve • Detector Design Strategies • Likelihood Ratio Test 13.3 Signal Classification 13.4 ... provides a brief and limited overview of some of the theory and practice of signal detection and classification The focus will be on the Gaussian observation model For more details and examples see...

Ngày tải lên: 05/11/2013, 17:20

... Temporal Signals in Gaussian Noise Signal Detection: Known Gains • Signal Detection: Unknown Gains • Signal Detection: Random Gains • Signal Detection: Single Signal 13.6 Spatio-Temporal Signals Detection: ... 13 Signal Detection and Classification 13.1 Introduction 13.2 Signal Detection The ROC Curve • Detector Design Strategies • Likelihood Ratio Test 13.3 Signal Classification 13.4 ... provides a brief and limited overview of some of the theory and practice of signal detection and classification The focus will be on the Gaussian observation model For more details and examples see...

Ngày tải lên: 05/11/2013, 17:20

... Temporal Signals in Gaussian Noise Signal Detection: Known Gains • Signal Detection: Unknown Gains • Signal Detection: Random Gains • Signal Detection: Single Signal 13.6 Spatio-Temporal Signals Detection: ... 13 Signal Detection and Classification 13.1 Introduction 13.2 Signal Detection The ROC Curve • Detector Design Strategies • Likelihood Ratio Test 13.3 Signal Classification 13.4 ... provides a brief and limited overview of some of the theory and practice of signal detection and classification The focus will be on the Gaussian observation model For more details and examples see...

Ngày tải lên: 08/11/2013, 12:15

... General Binary Detection with Unwanted Parameters 10.6.1 Signals with Random Phase 10.6.2 Signals with Random Phase and Amplitude 10.6.3 Signals with Random Parameters 10.7 Binary Detection in ... Distributions Random Processes Discrete-Time Random Process xv xvi Signal Detection and Estimation and part of Chapter on filtering, while Chapters 5, 6, 8, and can be covered in the course on Signal Detection ... Some Laws and Theorems If A and B are sets, then A Υ B and A Ι B are sets There is only one set ∅ and one universal set U, such that A Υ ∅ = A and A Ι U = A for any A Signal Detection and Estimation...

Ngày tải lên: 01/06/2014, 10:57

... acute phase serum, serodiagnosis and molecular assays for detection of viral RNA [5] Virus isolation though considered 'gold standard' is technically demanding and time consuming The most rapid ... isolation and evaluation with clinical samples MMP conceived the study and planned the work PVLR helped out to design and draft the manuscript and also revised it critically All authors read and approved ... successful isolation of Dengue type 2, and viruses (Table 1) RNA was extracted from culture supernatant and used as template in RT-PCR and nested PCR The RT-PCR and nested PCR revealed desired amplification...

Ngày tải lên: 20/06/2014, 01:20

... application of first-order signal cyclostationarity to the joint detection and classification of band-limited FSK and AM signals affected by additive Gaussian noise and phase, frequency, and time delay offsets ... 28] Signal Models Using approximate information about the signal bandwidth and carrier frequency, the received signal is down-converted EURASIP Journal on Advances in Signal Processing to baseband, ... CFs are, respectively, exploited for the detection and classification of FSK and AM signals A first-order cyclostationarity-based joint signal detection and classification algorithm is proposed,...

Ngày tải lên: 21/06/2014, 19:20

... −20 −30 −40 The detection and parameters estimation of multicomponent LFM signal can be realized by the CPF The principle of detection and parameters estimation of single LFM signal is presented ... stronger components, and we can improve the dependability of signal detection by the CLEAN technique 3.1 STATISTICAL PERFORMANCE Signal- to-noise ratio In the presence of signal s(n) without noise, ... Chan, and C K Chui, “Linear frequencymodulated signal detection using Radon-ambiguity transform,” IEEE Transactions on Signal Processing, vol 46, no 3, pp 571–586, 1998 [6] L Qi, R Tao, S Zhou, and...

Ngày tải lên: 21/06/2014, 22:20

... distinct groups - group I and II Group I consisted of PVM isolates detected and characterized in Italy, Germany, China, Poland and Russia and group II consisted of Canadian and US isolates Isolates ... further divided into two sub-groups - Ia, and Ib PVM isolates from China and Poland formed the group Ia and group Ib consisted of isolates from Italy, Germany and Russia Isolates in group II could ... sub-groups - IIa and IIb PVM isolates Idaho and Ca128 were considered to represent IIa and IIb, respectively Group IIa consisted of isolates Idaho, Ca508, Ca513, CL1 and CL3 and group IIb consisted...

Ngày tải lên: 12/08/2014, 04:21

... TaqMan probes used multiple probe primer sets for detection of all four serotypes of DENV [11] In the present study, we describe the development of a DENVspecific TaqMan based real-time PCR for detection ... design the primers and probe (Fig Fig 1) A generic reverse primer (nt 10708-10682), a forward primer (nt 10635–10658) and the probe (nt 10663–10679) were synthesized (Table 2) The probe was labelled ... at the 5'end and a minor groove binder (MGB) and a non fluorescent quencher at the 3'end The specificity of primers and probe were tested against the four serotypes of DENV, JE, WN, and CHIK viruses...

Ngày tải lên: 12/08/2014, 04:21

Ngày tải lên: 29/11/2014, 06:59

... Mr Zhang Yanyuan and Madam Lin Aiping, my grandmother Madam Qu Juying, my late grandfather Mr Lin Yunhu and all my relatives for their continuous and infinite love, support and encouragement ... analyte and induce a chemical signal; and a transducer that can translate the chemical signals into useful analytical signals (Fig 2.3) Fig.2.3 Schematic representation of the composition and function ... weathering, flaking, chalking, and dust Mild lead poisoning can cause anemia The victim may have headaches and sore muscles, and may feel generally fatigued and irritable (Harrison and Laxen 1981) High...

Ngày tải lên: 09/09/2015, 17:52

... Bioinformatics and cheminformatics tools are explored to make drug research and development more efficient and effective To help achieve this purpose, this work on "Development of Database and Computational ... libraries: model development and experimental test 120 6.1 Background 120 6.2 Materials and methods 123 6.2.1 6.3 Compound collections and construction of training and testing ... bioinformatics and cheminformatics are extensively explored to increase the efficiency and reduce costs of drug discovery and development Bioinformatics tools such as database and computational...

Ngày tải lên: 10/09/2015, 09:04

... sepsis and septic shock); ii) acute coronary syndrome (myocardial infarction and unstable angina); and iii) all other admission diagnoses This division was made due to the fact that sepsis and acute ... contributions IG conceived and organized the study, participated in patients' inclusion and follow-up, performed statistical analyses, analyzed results and wrote the manuscript AVB and VG participated ... diabetes and hyperglycaemia on infectious disease hospitalisation and outcome Diabetologia 2007, 50:549-554 Christiansen C, Toft P, Jorgensen HS, Andersen SK, Tonnesen E: Hyperglycaemia and mortality...

Ngày tải lên: 25/10/2012, 10:02

... BANKCARD AND ACTUAL STATE OF AGRIBANK CARDS 2.1 Introduction of Vietnam Bank for Agriculture and Rural Development (Agribank) - Thanh Xuan branch .9 2.1.1 Foundation and development ... Vietnam Bank for Agriculture and Rural Development (Agribank) - Thanh Xuan branch 2.1.1 Foundation and development of Agribank Thanh Xuan In 2007, Vietnam Bank for Agriculture Development - Thanh Xuan ... use the card within demand deposit account balance and/ or overdraft for payments of merchandises or services or for cash withdrawal/advance and other services at ATM/EDC and other cash disbursement...

Ngày tải lên: 09/11/2012, 15:31

... Indeed, some form of change detection is also needed In this section, we'll take what we've learned about locking and detection and formulate two pessimistic solutions and one optimistic solution ... statement and commits: update set where and person first_name = 'Tim' person_id = first_name = 'Tom'; Session two then executes the following UPDATE statement and commits: update set where and person ... tactics you can employ for detection Let me clarify that we are no longer discussing locking, but detection Detection is mutually exclusive of locking The first two detection tactics we will discuss...

Ngày tải lên: 29/09/2013, 09:20