hybridization scanning and data analysis

... plant assays LZ, WJ and YQH performed the bioinformatic analysis JLT, YQH and BC performed CC and other data analyses JLT, YQH and LZ wrote the paper All authors have read and approved the final ... NucleoSpin Extract II kit, resuspended in elution buffer and checked for its optical density Microarray hybridization, scanning and data analysis Construction of the whole-genome microarray of ... correlation analysis between the strain-plant reaction and the gene distribution pattern of strains to screen avr candidates and then ascertained the avirulence function of the candidates by...

Ngày tải lên: 14/08/2014, 08:20

... by the "right hand rule" while the magnitude depends on the rate of change of the x- and ycomponents of the vector field with respect to y and x 19 Numerical Methods and Data Analysis The curl ... is 27 Numerical Methods and Data Analysis determined by the analyst and he must be careful not to aim too high and carry out grossly inefficient calculations, or too low and obtain meaningless ... eliminated concerns of random error, but round off error can be a persistent problem Numerical Methods and Data Analysis The extreme speed of contemporary machines has tremendously expanded the scope...

Ngày tải lên: 20/12/2013, 19:15

... from them The union of A and B is the set A ∪ B = {x ∈ S : x ∈ A or x ∈ B} and the intersection of A and B is the set A ∩ B = {x ∈ S : x ∈ A and x ∈ B} Notice that unions and intersections are symmetric ... left hand and another on your right hand How many ways can this be done? First, suppose that you wear the diamond ring on your left hand Then there are three rings available for your right hand: ... such hands Hence, P (A) = #(A) 37 = 40 = = 0004 #(S) 2470 (c) One hand of four cards is dealt to Arlen and a second hand of four cards is dealt to Mike What is the probability that Arlen’s hand...

Ngày tải lên: 16/01/2014, 16:33

... Overview of Power Analysis: Thinking More Critically About Research and Data Analysis 400 ,) Knowledge Base 402 Planning for Data Analysis: Developing a Before and After Data Collection Analysis Plan ... Interquartile and the Semi-Interquartile Range 153 Variance and Standard Deviation 155 Sample Variance and Standard Deviation 157 Homogeneity and Heterogeneity: Understanding the Standard Deviations ... Calcuklting Variance and Standard Deviation from a Data Array 160 Population Variance and Standard Deviation 161 Looking Ahead: Biased and Unbiased Estimators of Variance and Standard Deviation 162...

Ngày tải lên: 29/03/2014, 04:21

... Term and Overall Capability 8.3 Capability Analysis for Normal Data 8.4 Capability Analysis for Non Normal Data 8.5 Capability Comparison using the Assistant 313 313 318 319 329 340 Correlation and ... hear and I forget I see and I remember I and I understand’ This proverb sums up the spirit of this book as it is very practical and it involves the reader Every chapter contains examples and exercises ... endpoint of the analysis The example and exercise data sets can be downloaded from the Wiley publishing website, www.wiley.com/go/six_sigma_methodology Problem Solving and Data Analysis using...

Ngày tải lên: 05/05/2014, 16:46

... if the data presents highly anisotropic features, the ridgelet transform (Cand`s and Donoho, 1999; Cand`s and Donoho, 1999) e e or the curvelet transform (Donoho and Duncan, 2000; Cand`s and Donoho, ... processing and pattern recognition is available on-line (Campbell and Murtagh, 2001) Data analysis texts of importance include Bishop (1995), and Ripley (1995) 1.2 Transformation and Data Representation ... methods, and by dimensionality mapping methods Multiband images can be taken as a particular case Such methods are pivotal in Chapter on multichannel data, on catalog analysis, and on data storage and...

Ngày tải lên: 05/06/2014, 11:59

... one on theory and one on data analysis, seems to me artificial Furthermore, many students take only one course in statistics and not have time for two or more Analysis of Data and the Practice ... equally likely, and P(A) is not EXAMPLE B A black urn contains red and green balls and a white urn contains red and green balls You are allowed to choose an urn and then choose a ball at random from ... Congress Cataloging-in-Publication Data Rice, John A., Mathematical statistics and data analysis / John A Rice —2nd ed p cm Includes bibliographical references and indexes ISBN 0-534-20934-3 (acid-free)...

Ngày tải lên: 12/06/2014, 16:26

... contributions MS and JMG designed the assays and strategies for its analysis, MS performed all library preparation and characterization, MS, DL and MP performed bisulfite validation studies, while QJ and ... strengths of MSCC and HELP-seq/Methylseq, and the supporting analytical workflow that maximizes the quantitative capabilities of the data generated Results and discussion Library preparation and sequencing ... workflow in Figure and an example of a UCSC genome browser representation of HELP-tagging data in Figure S1 in Additional file All data are available through the Gene Expression Omnibus database (accession...

Ngày tải lên: 09/08/2014, 20:21

... Chapters 4, 6, and 8, the reversible formation of binary complexes between enzymes and ligand molecules (i.e., substrates and inhibitors) is a critical aspect of both enzymatic catalysis and enzyme ... peptide carbon and the oxygen, and single-bond character between the peptide carbon and nitrogen atoms Table 3.2 provides typical bond lengths for carbon—oxygen and carbon—nitrogen double and single ... protein structure (Chapter 3) and enzyme—ligand binding (Chapters 4, 6, and 8); these are electrostatic interactions, hydrogen bonding, hydrophobic interactions, and van der Waals forces Here...

Ngày tải lên: 13/08/2014, 07:20

... results from a ligand binding experiment; the data show the effects of nonspecific ligand binding on the titration data Triangles illustrate the typical experimental data for total ligand binding, ... make visual inspection of the binding data much easier and are highly recommended for presentation of such data GRAPHIC ANALYSIS OF EQUILIBRIUM LIGAND BINDING DATA 89 Semilog plots allow for rapid ... standard equation for a straight line, we have: y : (mx) ; b (4.31) GRAPHIC ANALYSIS OF EQUILIBRIUM LIGAND BINDING DATA 91 Figure 4.9 Double-reciprocal plot for the data in Figure 4.3 The data...

Ngày tải lên: 13/08/2014, 07:20

... at latter time results from free ligand depletion from the column due to the binding events [Data redrawn from Hummel and Dreyer (1962).] bound and free ligand concentrations Hence, during protein ... Fless, G., Scanu, A., and Copeland, R A (1993) J Protein Chem 12, 39 Bell, J E., and Bell, E T (1988) Proteins and Enzymes, Prentice-Hall, Englewood Cliffs, NJ Campbell, I D., and Dwek, R A (1984) ... Haldane and K G Stern, Eds., Steinkopf, Dresden, pp 119ff Zeeberg, B., and Caplow, M (1979) Biochemistry, 18, 3880 Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis...

Ngày tải lên: 13/08/2014, 07:20

... J., Fersht, A R., Blow, D M., and Winter, G (1983) Biochemistry, 22, 3581 Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis Robert A Copeland Copyright  2000 by Wiley-VCH, ... T.-Y., Stein, R L., and Hagmann, W K (1993) J Med Chem 36, 4293 Cleland, W W (1967) Adv Enzymol 29 1—65 Copeland, R A (1991) Proc Natl Acad Sci USA 88, 7281 Cornish-Bowden, A., and Wharton, C W ... these powerful techniques REFERENCES AND FURTHER READING Bell, J E., and Bell, E T (1988) Proteins and Enzymes, Prentice-Hall, Englewood Cliffs, NJ Briggs, G E., and Haldane, J B S (1925) Biochem...

Ngày tải lên: 13/08/2014, 07:20

... intensity of the quinine sulfate standard at 453 nm, when the same fluorometer is used to excite both sample and standard, at the same wavelength Of course, both sample and standard measurements must be ... Biochem J 308, 305 Enzymes: A Practical Introduction to Structure, Mechanism, and Data Analysis Robert A Copeland Copyright  2000 by Wiley-VCH, Inc ISBNs: 0-471-35929-7 (Hardback); 0-471-22063-9 ... of enzyme catalysis and on the interactions of enzymes with ligands, such as substrates and inhibitors Chapter provided the basis for determining the kinetic constants k and K from initial velocity...

Ngày tải lên: 13/08/2014, 07:20

... choosing an appropriate column packing and in handling and using the material correctly is provided by the manufacturers The analysis of peaks from ion exchange and size exclusion columns is identical ... — contaminating protein bands may obscure the analysis of the substrate and product bands on the gel A common strategy in these cases is to perform Western blotting analysis using an antibody ... elucidation of enzyme mechanisms can be found in the reviews by Cleland and coworkers (Cleland et al., 1977) and by Northrop (1975), and in the recent compilation of selected articles from Methods...

Ngày tải lên: 13/08/2014, 07:20

... constants for [E] and [ES] by giving them separate symbols, such as K and # K , K and K , and K and K (the subscripts in this latter nomenclature #1 '  refer to the effects on the slope and intercept ... inhibitor and the modification of K by substrate The values of and provide information on the degree of modification that one ligand (i.e., substrate or inhibitor) has on the binding of the other ligand, ... methods for measuring equilibrium binding between proteins and ligands, discussed in Chapter 4, include equilibrium dialysis, and chromatographic and spectroscopic methods New instrumentation based...

Ngày tải lên: 13/08/2014, 07:20

... between v and v , and t is time  Morrison and Walsh (1988) have provided explicit mathematical expressions for v and v in the case of a competitive slow binding inhibitor, illustrating  that v and ... equations, the relationships between K  and K , and between K*  and K*, are the same as those presented in Chapters and for the relationships between K  and K for the different modes of inhibition ... Malcolm and Radda (1970) and Anderton and Rabin (1970) for examples of this approach 10.5.3.3 Affinity Labels Covalent modifying groups can often be incorporated into substrate analogues and other...

Ngày tải lên: 13/08/2014, 07:20

... package for data management and graphic display of enzyme kinetic data and other scientific data graphing This program has extensive preprogrammed routines for enzyme kinetic analysis and allows ... to explain allostery in enzymes and other ligand binding proteins The first, the simple sequential interaction model, proposed by Koshland and coworkers (Koshland et al., 1966), is in some ways ... fashion: data for a noncooperative enzyme; squares and triangles, data for enzymes displaying positive cooperativity Each solid line through the data represents the best fit of an individual data...

Ngày tải lên: 13/08/2014, 07:20

... the study, the data collection method, instruments as well as process of data collection and data analysis IV Data analysis and discussion This focuses on presenting, analyzing and discussing ... the data obtained from the survey questionnaires 28 CHAPTER IV- DATA ANALYSIS AND DISCUSSION 4.1.Questionnaires 4.1.1 Data analysis of the students’ experiment questionnaires 4.1.1.1 Data analysis ... methods of data collection and analysis in order to maximize its vavidity and reliability This claim would be subtaintiated in this as the participants, the instruments as well as the data analysis...

Ngày tải lên: 12/04/2013, 16:19

... some red and some green candies has a total of 60 candies in it The ratio of the number of green to red candies is 7:8 How many of each color are there in the bag? The sum of a number x and four ... remaining event by adding the known probabilities together and subtracting their total from – DATA ANALYSIS, STATISTICS, AND PROBABILITY – Graphs and Tables Inc rea se se rea Inc se a re Circle graphs ... Find the average of 9, 4, 7, 6, and 9+4+7+6+4 ᎏᎏ 30 = ᎏ5ᎏ = The average is (Divide by because there are numbers in the set.) 418 – DATA ANALYSIS, STATISTICS, AND PROBABILITY – To find the median...

Ngày tải lên: 02/11/2013, 17:20