human endolymphatic sac cell line

... action of extracellular nucleotides on the human lung adenocarcinoma cell line LXF-289, which is derived from solid lung adenocarcinoma Extracellular nucleotides strongly attenuated the cell cycle ... MAPKs extracellular signal-related kinase ⁄ (ERK1 ⁄ 2) and p38 Our findings underline the importance of extracellular nucleotides in the specific modulation of cell proliferation Results Extracellular ... ADP on cell cycle progression of LXF-289 cells (A) Accumulation of LXF-289 cells in the S phase LXF-289 cells were incubated with ATP or ADP (10–100 lM) for 24 h, and the distribution of cells...

Ngày tải lên: 07/03/2014, 12:20

... cancer cell lines ERa is potentially involved in miR-22-mediated repression of ERa-positive breast cancer cell growth To investigate the role of ERa in miR-22-mediated repression of human cancer cell ... breast cancer cell lines and surgical specimens It was interesting to find that downregulation of miR-22 expression occurs frequently, not only in ERa-positive human breast cancer cell lines, but ... detection primers for ESR1 and GAPDH are included in Table S2 Cell lines and cultures Five breast carcinoma cell lines were obtained from the Cell Resource Center of Peking Union Medical College (Beijing,...

Ngày tải lên: 22/03/2014, 21:20

... PKCh was detected in L-MAT cells at both mRNA (Fig 3A) and protein (Fig 3B) levels HepG2, a human hepatoma cell line, was used as a negative control for PKCh In HepG2 cells, neither PKCh mRNA nor ... TCDD-induced apoptosis in the human T -cell line, L-MAT, because AhR does not exist in L-MAT [7,8] In view of our previous findings and the very high susceptibility to TCDD among T -cell lines (A Hossain, unpublished ... many PKCh functions in T cells [28] Rottlerin has also been shown to block the activationinduced cell death process in T cells, indicating a functional role for PKCh in the cell- death mechanism [19,20]...

Ngày tải lên: 23/03/2014, 13:20

... purchased from Promega Cell culture The human hepatocellular carcinoma cell line, QGY-7703, was kindly provided by D Lu (Dept of Neurology, Stanford University, Palo Alto, CA, USA) The cells were maintained ... containing the extracellular domain (amino acids 114–281) of the human liver cancer cell line, QGY-7703 [27], we treated the cells with increasing concentrations of TRAIL and then analysed cell viability ... Dan, L & Yong, X (2001) Taxol induced Bcl-2 protein phosphorylation in human hepatocellular carcinoma QGY-7703 cell line Cell Biol Int 25, 261–265 28 Jo, M., Kim, T.H., Seol, D.W., Esplen, J.E.,...

Ngày tải lên: 23/03/2014, 17:22

... gynecological cancer cell lines Methods Cell culture All the cell lines used were described in Table [20-45] and obtained from Cell Lines Service (Eppelheim, Germany) Briefly, all cells were cultured ... comparison to the abovementioned cell lines, BeWo cells could be the best choice for future experiments concerning chorioncarcinoma cell lines and the Ishikawa cell line concerning endometrial carcinoma, ... at least one of the cell lines For MMP-8, -12 and -21 only very weak mRNA expression could be observed in single cell lines Nine MMPs, which were present in most of the cell lines, were chosen...

Ngày tải lên: 28/03/2014, 14:20

... and discussion line compared to human immortalized nasopharyngeal epithelial cell line NP69 by quantitative RT-PCR method (Fig 1A), which indicated that CNE2 cell line is a suitable cell model for ... various molecules across the cellular membrane, and are confirmed to be associated with anticancer drug transporter [5,6] Cell lines and animals The human NPC cell lines CNE1, CNE2, 5–8F, 6–10B, ... Detection of ABCC2 mRNA levels in NPC cells by Quantitative RT-PCR Expression of ABCC2 mRNA in NPC cell lines was detected compared to that in NP69 cell line Total RNA was isolated by using Trizol...

Ngày tải lên: 18/06/2014, 15:20

... indicating that only a small fraction of the cells exhibit methylation at any site within the HESC cell lines These sites were poorly conserved across the three cell lines, suggesting that they may arise ... epigenomics of cancer Cell 2007, 128:683-692 19 20 21 22 Hemberger M, Dean W, Reik W: Epigenetic dynamics of stem cells and cell lineage commitment: digging Waddington’s canal Nat Rev Mol Cell Biol 2009, ... Gnirke A, Eggan K, Meissner A: Reference maps of human ES and iPS cell variation enable highthroughput characterization of pluripotent cell lines Cell 2011, 144:439-452 Lister R, Pelizzola M, Kida...

Ngày tải lên: 09/08/2014, 23:20

... Multilineage potential of adult human mesenchymal stem cells Science (New York, NY) 1999, 284:143-147 Woodbury D, Reynolds K, Black IB: Adult bone marrow stromal stem cells express germline, ... mesenchymal stem cell in vitro BMC cell biology 2006, 7:14 Hung SC, Chen NJ, Hsieh SL, Li H, Ma HL, Lo WH: Isolation and characterization of size-sieved stem cells from human bone marrow Stem cells (Dayton, ... embryonic stem cells toward a cardiac lineage and early heart development Developmental cell 2006, 11:535-546 31 Darr H, Mayshar Y, Benvenisty N: Overexpression of NANOG in human ES cells enables...

Ngày tải lên: 10/08/2014, 05:21

... http://www.jeccr.com/content/30/1/15 tissue cells, binds to receptors in extracellular matrix such as hyaluronic acid (HA) and laminin, and mediates cell- cell and cell- matrix adhesion [12,13] The present ... imaging The experiment was repeated times Western Blot The cell line RMG-I was originated from ovarian clear cell cancer tissues The cell line RMG-I-H with high expression of a1, 2-FT and Lewis ... randomly selected to count 200 cells: the round and bright cells were counted as non-spreading cells; the oval cells with pseudopods were counted as spreading cells Irrelevant control antibodies...

Ngày tải lên: 10/08/2014, 10:21

... expressed in human tissues and cell lines, and high endogenous expression of JMJD2A mRNA was found in several cell types, including human T -cell lymphotropic virus 1infected cell lines, the HT1376 ... lymphotropic virus 1infected cell lines, the HT1376 bladder carcinoma cell line, the U2OS osteosarcoma cell line and the prostate cancer cell line [7,8] However, there are rare literatures focusing on the ... 5’-UAUCUUGAGUUGAUAACUC-3’ Cell transfection Human breast cancer cell line MDA-MB-231 in this research was preserved in our laboratory At 24 h before transfection, MDA-MB-231 cells in logarithmic growth...

Ngày tải lên: 10/08/2014, 10:21

... 2.2 Cell lines MCF-7, MDA-MB-231 and BT-474 breast cancer cell lines as well as MCF10A normal breast epithelial cell line were purchased from ATCC (Manassas, VA, USA) MCF-7 and MDA-MB-231 cells ... non-small cell lung cancer cells (Jafri et al., 2010), paclitaxel or doxorubicin in KBM-5 human myeloid cells (Sethi et al., 2008), and gemcitabine or oxaliplatin in HPAC human pancreatic cancer cells ... cells (Hussain et al., 2011), DLD-1 human colon cancer cells (El-Najjar et al., 2010) and C4-2B prostate cancer cells (Koka et al., 2010) The effect of TQ on ROS production in breast cancer cells...

Ngày tải lên: 10/09/2015, 09:09

... mechanisms 5342 Human cell lines used were HeLa cervical carcinoma cells, HepG2 hepatoma cells, K562 and YN-1 erythroleukemia cells, Jurkat T-lymphocyte cells, KG1 myeloid cells, H146 small cell lung ... expressed in all eight cell lines (Fig 1A,B) We also measured the heme content of these cell lines: it was about twofold higher in YN-1 and K562 erythroleukemia cells than in the other cell types (Fig ... SnPP on cellular heme content in human cell lines YN-1 erythroleukemia, HeLa cervical cancer, and HepG2 hepatoma cells were treated with mM SA (A) or 50 lM SnPP (B) for 48 h, and the cellular...

Ngày tải lên: 19/02/2014, 05:20

... intracellular heme level in certain human cell types under hypoxia Experimental procedures Cell culture and hypoxia study The human cell lines used were KG1 myeloid cells, K562 erythroid cells, ... of hypoxia on HO-1 and HO-2 expression in human cell lines We initially analyzed the effects of hypoxia on the expression of HO-1 and HO-2 in human cell lines of Reduced expression of heme oxygenase-2 ... expression of HO-1 in several types of human cell [13,14], but conversely induces it in cultured human dermal fibroblasts [15] and a retinal pigment epithelial cell line [16] Thus, expression of HO-1...

Ngày tải lên: 19/02/2014, 06:20

... (thick lines) as first step in permeabilized cells (C) Histograms show ferritin expression in nonpermeabilized (thin lines, – saponin) and permeabilized cells (thick lines, + saponin) cells cultured ... anti-(mouse Igs) Cells were acquired immediately in a FACSCalibur and analyzed using CELLQUEST Histogram shows cell surface expression of acrolein adducts in HepG2 cells cultured without (thin line, – ... For intracellular staining, cells were first permeabilized with 0.2% saponin Labeled cells were acquired in a FACSCalibur and analyzed using the CELLQUEST software (A) Histograms show cell surface...

Ngày tải lên: 19/02/2014, 16:20

... oligomer on cellular growth and on nuclear protein binding in human lymphocytes (A) Effect of GT oligomer on cellular growth or viability A total of 104 CCRF-CEM cells or peripheral normal human lymphocytes ... USA) into viable cells [19] The percentage of viable cells in the treated samples was estimated, taking, as 100% cellular viability, that of the internal-control nontreated cells Preparation ... of eEF1A in normal and cancer cells In order to compare the binding properties of the nuclear eEF1A in normal cells compared with those in cancer CCRF-CEM cells, human lymphocytes were isolated...

Ngày tải lên: 21/02/2014, 00:20

... cell adhesion to HUVECs after treatment with insulin, LY294002 or transfection with AS-PKB cDNA HUVEC, human umbilical vein endothelial cells; UnC, untreated control H7721 cells; Ins, H7721 cells ... invasive activity of the cells Inhibition of cell adhesion, migration and invasion with monoclonal antibody For inhibition of cell adhesion, migration and invasion, H7721 cells were preincubated ... LY294002, H7721 cells treated with 15 lM LY294002; LY294002 + Ins, H7721 cells treated with both LY294002 and insulin; Mock, H7721 cells transfected with pcDNA3 vector; AS-PKB, H7721 cells transfected...

Ngày tải lên: 21/02/2014, 00:20

... capacity of cells The human cancer cell lines chosen included one epidermoid carcinoma cell line, three mammary tumour cell lines and one human pancreas adenocarcinoma All five cell lines exhibited ... with different characteristics such as a human epidermoid carcinoma cell line (A-431) and a human mammary tumour cell line (MCF-7) In order to allow us to delineate any potential uptake mechanism ... cancer cell lines; and (b) if uptake could be proven, to describe the general nature of this potentially important physiological phenomenon Results human cells We initially chose cell lines with...

Ngày tải lên: 16/03/2014, 06:20

... oligodeoxynucleotides in human cancer cells DNA Cell Biol 22, 685–696 15 Scaggiante B, Morassutti C, Dapas B, Tolazzi G, Ustulin F & Quadrifoglio F (1998) Human cancer cell lines growth inhibition ... intracellular medium (data not shown) To test the effect of these oligomers on cellular growth we performed a cytotoxicity assay The oligomers were applied to human lymphoblast CCRFCEM cells ... on haematopoietic cancer cell lines, whereas the G-rich GT oligomer GRO29A was successfully used by Bates to significantly inhibit the growth of a variety of human cancer cells derived from solid...

Ngày tải lên: 16/03/2014, 13:20

... the major cellular site for ROS formation, reduces enzymatic and soluble intracellular antioxidant defences but not ROS flux in the studied q0 cells, and that there are cell line- to -cell line variations ... on three human tumour cell lines, with (q+) and without (q0) mtDNA GSH concentration was significantly decreased in all three mtDNA depleted cell lines compared to parental lines with mtDNA; the ... muscle-derived q0 cells, compared with the parental q+ cells, but the absolute value was significantly higher in bone than in muscle q0 cells No differences were evident in lung q+ and q0 cell lines (Fig...

Ngày tải lên: 16/03/2014, 18:20

... independent HUT102B2 cells were derived from a human adult T cell lymphoma associated with the human T cell lymphotropic virus I (HTLV-I) [24] MT-1 is also an adult T cell leukemia cell line associated ... IL-2 stimulated tyrosine phosphorylation of T cell signaling molecules EXPERIMENTAL PROCEDURES Cell lines and mAbs The Kit225 K6 cell line is a human T cell with a helper/ inducer phenotype and an ... T cell types (‡ 105 per cell) , characteristic of leukemic or activated T cells HLA-DR was abundant on all cell lines (‡ · 105 copies per cell) Surface density of class I HLA was low on MT-1 cells...

Ngày tải lên: 17/03/2014, 23:20