human colon cancer cell lines

... breast cancer cell lines ERa is potentially involved in miR-22-mediated repression of ERa-positive breast cancer cell growth To investigate the role of ERa in miR-22-mediated repression of human cancer ... Frequent downregulation of miR-22 expression in ERa-positive breast cancer cell lines and tumor specimens (A) For breast cancer cell lines, the expression levels of mature miR-22 were determined by ... and liver cancer cells by targeting multiple cell cycle oncogenes [28] and miR-34b and miR-34c have a cooperative negative effect on proliferation and colony formation of ovarian cancer cells [6],...

Ngày tải lên: 22/03/2014, 21:20

... gynecological cancer cell lines Methods Cell culture All the cell lines used were described in Table [20-45] and obtained from Cell Lines Service (Eppelheim, Germany) Briefly, all cells were cultured ... the ovarian cancer cell lines OAW-42, BG-1 and in the teratocarcinoma cell line PA-1 compared to already performed examinations of endometrial, cervix or choriocarcinoma cancer cell lines, as already ... in HeLa cells Lastly, all three cervical cancer cell lines four protein bands of approximately 62, 50, 48 and 46 kDa were found for MMP-28 Expression of MMPs in chorioncarcinoma cell lines Albeit...

Ngày tải lên: 28/03/2014, 14:20

... transfer Methods Cell culture We used two colon cancer cell lines: the human HT29 and the murine DHDK12 pro-b (Pr Martin, Dijon; France) cell lines Cell lines were cultured in DMEM medium containing ... transfer improvement between DHDK12 and HT29 cell lines after cell synchronization by MTX These two colon cancer cell lines exhibit a different pattern of cell cycle distribution after synchronization ... retroviral gene transfer differs between the two colon cancer cell lines used in this study, further investigations in more colon cancer cell lines are needed to draw definitive conclusion on...

Ngày tải lên: 10/08/2014, 10:21

... cells (Hussain et al., 2011), DLD-1 human colon cancer cells (El-Najjar et al., 2010) and C4-2B prostate cancer cells (Koka et al., 2010) The effect of TQ on ROS production in breast cancer cells ... non-small cell lung cancer cells (Jafri et al., 2010), paclitaxel or doxorubicin in KBM-5 human myeloid cells (Sethi et al., 2008), and gemcitabine or oxaliplatin in HPAC human pancreatic cancer cells ... 2.2 Cell lines MCF-7, MDA-MB-231 and BT-474 breast cancer cell lines as well as MCF10A normal breast epithelial cell line were purchased from ATCC (Manassas, VA, USA) MCF-7 and MDA-MB-231 cells...

Ngày tải lên: 10/09/2015, 09:09

... effects on haematopoietic cancer cell lines, whereas the G-rich GT oligomer GRO29A was successfully used by Bates to significantly inhibit the growth of a variety of human cancer cells derived from solid ... of apoptosis Cancer Res 64, 6603–6609 14 Jing N, Li Y, Xu X, Sha W, Li P, Feng L & Tweardy DJ (2003) Targeting Stat3 with G-quartet oligodeoxynucleotides in human cancer cells DNA Cell Biol 22, ... H, Mayo J & Boyd M (2001) Feasibility of a high-flux anticancer drug screen using a diverse panel of cultured human tumor cell lines J Natl Cancer Inst 83, 757–766 Bradford MM (1976) A rapid and...

Ngày tải lên: 16/03/2014, 13:20

... our group to reduce cell growth in T-84 human colon cancer cells, and in SP-1 keratinocyte cell line, under both normoxia and hypoxia; however, drug toxicity was greater in cells exposed to hypoxia ... [18] Cell culture and treatments FHs74Int normal human intestinal cells were cultured in Hybri-Care medium supplemented with 30 ng/ml epidermal growth factor HCT116 (p53+/+) human colon cancer cells ... apoptosis in several murine and human cancer cell lines [4,5,8] This is our first attempt to understand the role of p53 and p21 in drug efficacy using colon cancer cells that are wildtype or null...

Ngày tải lên: 09/08/2014, 09:20

... AG, Berlin, Germany Cell lines and culture The following human cancer cell lines were used for experimentation: testicular cancer (H12.1, 2102EP, 1411HP, 1777NRpmet), colon cancer (HCT-8, HCT-15, ... Irinotecan in human colon cancer cell lines The combined drug effect of a parallel exposure to FWGE and either 5-FU, irinotecan or oxaliplatin was assessed in a panel of colon cancer cell lines The ... death induced by FWGE in a panel of cell lines In order to distinguish the mode of cell death induced by FWGE we treated a representative panel of human cancer cell lines with an IC90 of FWGE for...

Ngày tải lên: 10/08/2014, 10:21

... test lung cancer cell lines (black and gray) and four validation cell lines (white) was quantified by qPCR and compared to a normal control cell line (MRC-9) Watanabe et al Cancer Cell International ... principal component for the cell lines (PC1, PC2, and PC3), two of the three AD cells lines were close, whereas the third cell line was close in PC2 to the other two cell lines, but separate in PC1 ... close LC cell lines and three SC cell lines were different in PC1, with a negative value for LC and a positive value for SC The 86-2 cell line (LC) was different in PC3 from the SC cell lines Subtypes...

Ngày tải lên: 15/02/2014, 04:20

... concentration-dependent reduction in cell number and cell viability was observed in HCT116 and GEO cell lines cultured with CDO However, no changes to cell number or viability was observed in the cells treated with ... SW620 cells (>1600 nM) Results showed dose-dependent decreases in cell number and concomitant decreases in cell viability in the sensitive cell lines Flow cytometric analysis showed that this cell ... colorectal cancer cell lines; second, to investigate the effects of combining CDO with etoposide (VP16), 5-fluorouracil (5-FU) or SN38 on cell growth and viability; and third, to elucidate the cellular...

Ngày tải lên: 07/03/2014, 15:20

... by MTX in HT29 cells using cDNA arrays The expression profile of the 1176 genes included in the Atlas Human Cancer 1.2K Array (ClontechÒ) was analyzed in parental HT29 cells, HT29 cells treated ... gastric cancer Cancer Res 63, 7724–7732 39 Giovanella BC, Stehlin JS, Wall ME, Wani MC, Nicholas AW, Liu LF, Silber R & Potmesil M (1989) DNA topoisomerase I – targeted chemotherapy of human colon cancer ... prostate cancer Cancer Res 63, 2306–2311 49 Wilson KS, Roberts H, Leek R, Harris AL & Geradts J (2002) Differential gene expression patterns in HER2 ⁄ neu-positive and -negative breast cancer cell lines...

Ngày tải lên: 07/03/2014, 16:20

... “Sensitive” cell lines were defined as cell lines which were classified as an “early” or “moderate” responders to GSK1070916 treatment by cell cycle analysis (FACs) with a Ymin/T0 ratio of ≤ 0.5 Cell lines ... Defining Cell Sensitivity An analysis of cell line sensitivity to GSK1070916 was performed with the data generated from screening cell lines in cellular proliferation assays and from cell cycle ... hematological cell lines were responsive to GSK1070916 with a median EC50 of nM Since cancer cell death is a more desired phenotype, the in vitro response of 91 hematological cell lines were defined...

Ngày tải lên: 18/06/2014, 22:20

... “Sensitive” cell lines were defined as cell lines which were classified as an “early” or “moderate” responders to GSK1070916 treatment by cell cycle analysis (FACs) with a Ymin/T0 ratio of ≤ 0.5 Cell lines ... Defining Cell Sensitivity An analysis of cell line sensitivity to GSK1070916 was performed with the data generated from screening cell lines in cellular proliferation assays and from cell cycle ... hematological cell lines were responsive to GSK1070916 with a median EC50 of nM Since cancer cell death is a more desired phenotype, the in vitro response of 91 hematological cell lines were defined...

Ngày tải lên: 20/06/2014, 04:20

... sensitive cell lines Page of 11 Methods Cell lines and cell culture Human epithelial ovarian tumor cis-platin sensitive (A2780) cell line was obtained from Dr Denise Connolly (Fox Chase Cancer Center, ... and 5% CO The cell lines were sub-cultured on routinely basis every 3-4 days Cell viability assays A2780 and A2780/CP70 cell lines were cultured to test the responsiveness of each cell line to ... expression in human cancer cells Biochem Pharmacol 2008, 76:582-588 24 Hamid T, Malik MT, Kakar SS: Ectopic expression of PTTG1/securin promotes tumorigenesis in human embryonic kidney cells Mol Cancer...

Ngày tải lên: 20/06/2014, 08:20

... radiosensitivity Materials and methods Cell line culture and reagents Human colon cancer cell lines RKO (ATCC, USA), breast cancer cell line MCF-7 (KCLB, Korea), and normal colon cell line DDC-112 CoN (ATCC) ... radiosensitivy in prostate cancer cell lines In this experiment, RKO cell lines showed a 56% survival rate with radiation alone, while with SB, 47% survived In MCF-7 cell lines, radiation alone led ... determining radiosensitivity ated in MCF-7 cell lines This might be the reason why MCF-7 cell lines are more susceptible to HDAC inhibitor than RKO cell lines Histone deacetylation and DNA methylation...

Ngày tải lên: 09/08/2014, 04:21

... efficacy towards hypoxic tumor cells This is the first report of DCQ sensitization when combined with IR against human colon cancer cells All four human colon cancer cell lines were sensitive to DCQ+IR, ... quantified using LabWorks 4.0 software Results Cell cycle modulation in four human colon cancer cell lines under oxia To study cell cycle modulation by DCQ+IR, cells were incubated with DCQ (5 or 10 ... different human colon cancer cell lines (SW-480, HT29, HCT116 and DLD-1) Cells were treated with DCQ (0, 5, 10 μM), IR (2 Gy) or combinations Immediately after radiation or drug treatment, cells...

Ngày tải lên: 09/08/2014, 10:21

... indicating that only a small fraction of the cells exhibit methylation at any site within the HESC cell lines These sites were poorly conserved across the three cell lines, suggesting that they may arise ... epigenomics of cancer Cell 2007, 128:683-692 19 20 21 22 Hemberger M, Dean W, Reik W: Epigenetic dynamics of stem cells and cell lineage commitment: digging Waddington’s canal Nat Rev Mol Cell Biol ... Gnirke A, Eggan K, Meissner A: Reference maps of human ES and iPS cell variation enable highthroughput characterization of pluripotent cell lines Cell 2011, 144:439-452 Lister R, Pelizzola M, Kida...

Ngày tải lên: 09/08/2014, 23:20

... mesenchymal stem cell in vitro BMC cell biology 2006, 7:14 Hung SC, Chen NJ, Hsieh SL, Li H, Ma HL, Lo WH: Isolation and characterization of size-sieved stem cells from human bone marrow Stem cells (Dayton, ... co-cultured CD34+ hematopoietic stem cells with 3A6 cells, we noted the formation of cobblestone areas from hematopoietic cells that transmigrated beneath the layer of 3A6 cells (Figure 3C) Previously, ... normal human cells Science (New York, NY) 1998, 279:349-352 Shi S, Gronthos S, Chen S, Reddi A, Counter CM, Robey PG, Wang CY: Bone formation by human postnatal bone marrow stromal stem cells...

Ngày tải lên: 10/08/2014, 05:21

... enhance cell- cell adhesion, and inhibit cell apoptosis [11] Labarrière et al [27] also found that CD44v6 in mouse colon cancer cells contains H antigen Its fucose structure is involved in cell adhesion, ... induces integrin-mediated adhesion of colon cancer cell lines to endothelial cells by up-regulation of integrins and c-Met and activation of integrins J Cancer Res 1999, 59:4427-4434 36 Wielenga ... Clinical Cancer Research 2011, 30:15 http://www.jeccr.com/content/30/1/15 tissue cells, binds to receptors in extracellular matrix such as hyaluronic acid (HA) and laminin, and mediates cell- cell...

Ngày tải lên: 10/08/2014, 10:21

... alpha(ER a ), matrix metalloproteinase 9(MMP-9), cyclinD1, and on cancer cells invasion, proliferation and cell cycle cell in two cell lines were investigated Page of 11 Construction of shRNA expression ... Hai Ding An Corp in China for sequencing Methods Cell lines and culture Transfection with shRNA/MTA1 expression vector The human breast cancer cell lines MDA-MB-231 and MCF-7 were kindly supplied ... blot analysis Results in two breast cancer cells by Western blot ananlysis indicated that, ER alpha was recovered positive in ER-negative human breast cancer cell lines MDA-MB231, and protein levels...

Ngày tải lên: 10/08/2014, 10:21

... expressed in human tissues and cell lines, and high endogenous expression of JMJD2A mRNA was found in several cell types, including human T -cell lymphotropic virus 1infected cell lines, the HT1376 ... MDA-MB-231 cells Discussion As leading cause of cancer death among females, human breast cancer has the features of powerful invasive ability and early metastatic property Human breast cancer with ... 5’-UAUCUUGAGUUGAUAACUC-3’ Cell transfection Human breast cancer cell line MDA-MB-231 in this research was preserved in our laboratory At 24 h before transfection, MDA-MB-231 cells in logarithmic growth...

Ngày tải lên: 10/08/2014, 10:21