home physical functioning and health related quality of life
Báo cáo y học: "Neutrophils exhibit distinct phenotypes toward chitosans with different degrees of deacetylation: implications for cartilage repair" pdf
... used to generate a standard curve The value '% release of MPO' corresponds to the ratio of the amount of MPO released/the total amount of released and cellular MPO For the lactoferrin assay, PMNs ... whether 80 M and 95 M chitosan induce PMNs to degranulate, the release of the contents of the primary and secondary granules was determined by measuring the amount of MPO and lactoferrin released ... of 47.4, derived from the molar extinction coefficient of cytochrome c Release of myeloperoxidase and lactoferrin by polymorphonuclear neutrophils Degranulation was determined using the MPO and...
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Báo cáo hóa học: " Development and application of a biomarker assay for determining the pharmacodynamic activity of an antagonist candidate biotherapeutic antibody to IL21R in whole blood" pptx
... of IL21R and has shown efficacy in a mouse model of lupus [8] The goal of the biomarker strategy was to provide the means of avoiding toxicity due to unnecessarily high drug levels and lack of ... analysis and review and participated in the drafting of the manuscript DY co-led the Ab-01 team and was responsible for studies related to the characterization of the biological properties of the ... for each of the donors, and subsequently the mean and standard deviation were determined for each gene shown A: Percent inhibition in presence of Ab-01 B: Percent inhibition in presence of control...
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Báo cáo khoa học: " Prediction of clinical toxicity in locally advanced head and neck cancer patients by radio-induced apoptosis in peripheral blood lymphocytes (PBLs)" ppt
... Glanzmann C: Quality of life in patients cured from a carcinoma of the head and neck by radiotherapy: the importance of the target volume Int J Radiat Oncol Biol Phys 1999, 45:47-52 Page of 18 Munter ... the origin of the curve in the Y axis and determines the percentage of spontaneous cell death and b is the slope of the curve and determines the percentage of cell death induced at a determined ... http://www.ro-journal.com/content/5/1/4 AR, BP and MLl have made the selection of patients, the evaluation of clinical variables and grade of toxicity as well as all the aspects related with the patients selected,...
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Báo cáo y học: "Perforin, granzyme B, and FasL expression by peripheral blood T lymphocytes in emphysema" pptx
... this study and of others on the expression of activation markers by peripheral T lymphocytes [19-21], they showed that the percentage of peripheral CD3+ and CD8+ cells expressing perforin and granzyme ... emphysematous smokers with evidence of systemic inflammation will show higher expression of cytotoxic and activation markers such as perforin, granzyme B, and FasL than that of subjects with normal lung ... compared to 18S rRNA For each PCR reaction, µl of the 1/100 dilution of the cDNA stock was used for perforin, granzyme B, and FasL analyses, and µl of the 1/1000 dilution was used for 18S rRNA...
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Báo cáo y học: " Decrease of CD4-lymphocytes and apoptosis of CD14-monocytes are characteristic alterations in sepsis caused by ventilator-associated pneumonia: results from an observational study" ppt
... of healthy volunteers may dif- Figure Apoptosis of CD14-monocytes and of CD4-lymphocytes of healthy volunteers Induction of apoptosis of CD14-monocytes and inhibition of apoptovolunteers sis of ... of Acinetobacter baumannii and another of Pseudomonas aeruginosa isolated at a count of × 106 cfu/ ml or more from TBS of two different patients with VAP; and one of A baumannii and another of ... precisely, in Figure TNFα and IL-6 production from the supernatants of monocytes Concentrations of TNFα and IL-6 of supernatants of monocytes of patients with septhe supernatants of monocytes sis due...
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Báo cáo y học: " In Vitro impairment of whole blood coagulation and platelet function by hypertonic saline hydroxyethyl starch" docx
... cohort of patients we have chosen a model of in vitro dilution for standardization of study conditions to estimate the effects of Hanke et al Scandinavian Journal of Trauma, Resuscitation and Emergency ... http://www.sjtrem.com/content/19/1/12 Page of Figure Scanning electron microscopy of native platelets (panels A and C) and platelets from blood after 40% dilution HyperHaes (panels B and D) in 5400fold (panels A and B) and 2000fold ... information and written consent All volunteers were healthy and free of medication Blood was taken from a basilic vein using an 18gauge IV catheter and collected in both citrated and heparinzed...
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Báo cáo y học: "Gene expression response in target organ and whole blood varies as a function of target organ injury phenotype" ppsx
... Program of the National Institute of Health and the National Institute of Environmental Health Sciences and has been funded in part with Federal funds from the National Institute of Environmental Health ... GAB, JTA, MLC and ANH conceived, designed, and supervised various aspects of the study PEB, SOS and MLC participated in the in -life work/ tissue collection and RNA purification EKL and PH made ... use of genomic approaches to better understand the adverse effects of environmental and xenobiotic exposures on human injury and disease processes engendered a great deal of early enthusiasm and...
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Tài liệu Detection, Evaluation, and Treatment of High Blood Cholesterol in Adults pdf
... Features of ATP III similar to those of ATP I and II Identification of population groups, besides middle-aged men, for detection of high LDL cholesterol (and other lipid risk factors) and for ... as target of therapy Notwithstanding this diverse evidence, the ultimate proof of the benefits of lowering LDL cholesterol is through clinical trial A large number of clinical trials of cholesterol-lowering ... reported for each of the individual studies † See List of Studies appendix for listing of the full names of these clinical trials c Categories and classification of total cholesterol and LDL cholesterol...
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Báo cáo khoa học: Soluble recombinant CD69 receptors optimized to have an exceptional physical and chemical stability display prolonged circulation and remain intact in the blood of mice doc
... sequence (B) SDS ⁄ PAGE of CD68CQ65 (lanes and 3), CD69NG70 (lanes and 5), CD69NV82 (lanes and 7) CD69NS84 (lanes and 9), rat CD69 (lanes 10 and 11) and mouse CD69 (lanes 12 and 13) was performed ... Estimates of hydrodynamic radii of the expected molecular species were calculated with the software hydropro [26] The structures of monomers and dimers of the human CD69 in the present study, and as ... of the protein and the precipitant solution and each drop was equilibrated against mL of precipitant solution After optimization of poly(ethylene glycol) molecular weight and concentration, and...
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báo cáo hóa học:" Whole blood assessment of antigen specific cellular immune response by real time quantitative PCR: a versatile monitoring and discovery tool" potx
... expression of genes encoding cytokines and chemokines and anti HBsAg serum titers in vaccinated healthy donors Correlation Correlation between expression of genes encoding cytokines and chemokines and ... antigenic peptides in WB of healthy donors WB from two HLA-A0201+ healthy donors (panels A and B), seropositive for HCMV and EBV (300 μl) was incubated for hours in the presence of HCMV pp65495–503 ... reverse transcribed and amplified in the presence of primers and probes specific for β-actin house keeping gene and genes encoding different cytokines Expression of IFN-γ and IL-2 genes was significantly...
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báo cáo hóa học:" Discovery and implementation of transcriptional biomarkers of synthetic LXR agonists in peripheral blood cells" pptx
... activity of LXR modulator compounds in target organs Several candidate biomarkers of LXR agonist exposure and activity have been identified in peripheral blood, and two of these (ABCA1 and ABCG1) ... elevation of ABCA1 and ABCG1 mRNAs reflected the potency of agonists to activate LXR receptors, rats were treated with a range of doses of GW3965 (Figure 2D) or LXR-623 (Figure 2E) Since the potency of ... with 0, 15 and 50 mpk/day of LXR-623 for days (n = per dose group) Blood was collected on day of dosing, and RNA was prepared from whole blood for gene expression analysis of ABCA1 and ABCG1 qPCR...
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Báo cáo hóa học: " cDNA targets improve whole blood gene expression profiling and enhance detection of pharmocodynamic biomarkers: a quantitative platform analysis" ppt
... expression of thousands of genes [9,12,13] Another challenge is that the specificity and sensitivity of a given RNA profiling platform are affected by the abundance and variability of the globin ... for the understanding of disease and the development of disease treatments It is a ubiquitous tissue that interacts throughout the body and Page of 12 literally acts as a sensor of physiological ... drafting and editing of the manuscript SL completed the analysis of the protocol selection study, participated in the analysis of the SAHA data and participated in the drafting and editing of the...
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Báo cáo hóa học: "Quantification of newly produced B and T lymphocytes in untreated chronic lymphocytic leukemia patients" ppt
... confounding effect of tumor DNA derived from monoclonal B cells on the quantification of KRECs and TRECs, genomic DNA from PBMC of healthy donors with high and low number of KRECs and TRECs was serially ... EA: Design and standardization of PCR primers and protocols for detection of clonal immunoglobulin and T-cell receptor gene recombinations in suspect lymphoproliferations: report of the BIOMED-2 ... combinations of monoclonal antibodies The percentage of Th cells and CTL is obtained after gating on lymphocytes, that of naive Th cells, Treg, TEM and TCM after gating on Th cells, and that of thymicnaive...
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Báo cáo sinh học: "Interferon signaling patterns in peripheral blood lymphocytes may predict clinical outcome after high-dose interferon therapy in melanoma patients" potx
... important and diverse immunoregulatory functions These include promoting proliferation and clonal expansion of CD4 and CD8 T cells [17-20], enhancing antibody production of B cells [21,22], and increasing ... the responsibility of the authors and does not necessarily represent the official views of the National Cancer Institute or the National Institutes of Health Author details Dept of Medicine, Stanford ... classified as NED and exhibited no evidence of metastases at a minimum follow-up of 4.5 years In contrast, all of the MET patients developed metastatic disease within years and three of these were...
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Báo cáo sinh học: "Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" pot
... assessed by expression of CD69 and production of interleukin-2 (IL-2) [15] To feasibly and safely cause regression of carcinomas, the targets recognized by the chTCR is of paramount importance ... terminus of the scFv, extracellular and transmembrane portions of either mouse or human CD28 (etm28) are covalently attached to aid interaction between the chTCR and target and enable fluidity of the ... stimulated to proliferate with anti-CD3 and anti-CD28 Typically, only ~10% of cells (not shown) became infected as judged by expression of GFP, a component of the receptor construct A variety of techniques...
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báo cáo hóa học: " Vascular endothelial growth factor-A and chemokine ligand (CCL2) genes are upregulated in peripheral blood mononuclear cells in Indian amyotrophic lateral sclerosis patients" pot
... grant PI and clinical scoring CA Acquisition of data NKS Statistical analysis AA Interpretation and analysis of data, grant co PI and writing and editing of manuscript All authors read and approved ... appearance of first symptom of ALS and collection of sample ALS subjects were asked to provide all clinical and demographic details at the age of disease-onset Gupta et al Journal of Neuroinflammation ... Council of Medical Research), India, for providing funds (No 5/4 - 5/10/Neuro/2005 - NCD - I) Page of 15 16 Authors’ contributions PKG Acquisition of data and writing of manuscript SP inclusion of...
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báo cáo hóa học:" Interferon signaling patterns in peripheral blood lymphocytes may predict clinical outcome after high-dose interferon therapy in melanoma patients" pptx
... important and diverse immunoregulatory functions These include promoting proliferation and clonal expansion of CD4 and CD8 T cells [17-20], enhancing antibody production of B cells [21,22], and increasing ... the responsibility of the authors and does not necessarily represent the official views of the National Cancer Institute or the National Institutes of Health Author details Dept of Medicine, Stanford ... classified as NED and exhibited no evidence of metastases at a minimum follow-up of 4.5 years In contrast, all of the MET patients developed metastatic disease within years and three of these were...
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báo cáo hóa học:" Use of recombinant lentivirus pseudotyped with vesicular stomatitis virus glycoprotein G for efficient generation of human anti-cancer chimeric T cells by transduction of human peripheral blood lymphocytes in vitro" docx
... assessed by expression of CD69 and production of interleukin-2 (IL-2) [15] To feasibly and safely cause regression of carcinomas, the targets recognized by the chTCR is of paramount importance ... terminus of the scFv, extracellular and transmembrane portions of either mouse or human CD28 (etm28) are covalently attached to aid interaction between the chTCR and target and enable fluidity of the ... stimulated to proliferate with anti-CD3 and anti-CD28 Typically, only ~10% of cells (not shown) became infected as judged by expression of GFP, a component of the receptor construct A variety of techniques...
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báo cáo hóa học:" Use of Tranexamic acid is a cost effective method in preventing blood loss during and after total knee replacement" ppt
... preparation of the questionnaire and protocol, collection of data and writing the manuscript MU was involved in the overall supervision, preparation of the questionnaire and collection and analysis of ... 77.42% for HIV and 86.84% for HBV The prevalence of HIV is 0.001% and of HBV is 2.259% [30] The probability of receiving an infective unit P(R) per 10000 donations is 0.023 for HIV and 29.72 for ... design, analysis and was involved in critically reviewing the manuscript FA and MUC participated in the designed the Page of study and participated in the preparation of the protocol and data collection...
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báo cáo hóa học:" Use of Tranexamic acid is a cost effective method in preventing blood loss during and after total knee replacement" pdf
... preparation of the questionnaire and protocol, collection of data and writing the manuscript MU was involved in the overall supervision, preparation of the questionnaire and collection and analysis of ... 77.42% for HIV and 86.84% for HBV The prevalence of HIV is 0.001% and of HBV is 2.259% [30] The probability of receiving an infective unit P(R) per 10000 donations is 0.023 for HIV and 29.72 for ... design, analysis and was involved in critically reviewing the manuscript FA and MUC participated in the designed the Page of study and participated in the preparation of the protocol and data collection...
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