hiv s interactions with liver sinusoidal endothelial cells

... Stellate cells (SC) and liver sinusoidal endothelial cells (LSEC) Following intravenous administration of fluorescently labeled substances, sections were prepared as described in the Methods section ... microvessel endothelial cells [23], as well as in peripheral macrophages [24]).), suggesting that FITC-FSA is superior as a LSEC specific marker Conclusions This study showed that pig LSEC are ... stellate cells and lymphocytes) are unable to perform phagocytosis, suggest that absence of phagocytosis is not a valid way of distinguishing LSEC from other liver cells In other studies, positive...

Ngày tải lên: 13/08/2014, 13:20

... oxygen levels itself is a disfavourable environment for LSECs 12 10 6 hours 24 hours 48 hours Figure Porosity4measurements Porosity measurements Porosity analysis of LSECs seeded on coverslips at 6, ... glucose measurements in supernatants obtained from dishes with or without cells Time (h) Cell-free (20% O2) LSECs (20% O2) LSECs (5% O2) LSECs (20% O2) LSECs (5% O2) Lactate (mmol/L/106 cells) ... analysed using ImageJ software The results are expressed as mean ± S. D Isolation and culture of LSEC Preparation of highly purified rat liver sinusoidal endothelial cells was performed as previously...

Ngày tải lên: 13/08/2014, 13:20

... reviews have summarised the understanding of HIV- DC interactions [19,61]; so here we focus on discussing the latest progress in this field DC-mediated HIV- 1 trans infection of CD4+ T cells is dependent ... Conversely, a recent study has suggested that Langerhans cells isolated from the oral cavity of HIV1 positive individuals not act as reservoirs for HIV- 1, despite HIV- 1 detection within whole tissue ... DC-mediated HIV- 1 transmission to CD4+ T cells [66,71,72] Immature DCs are capable of constitutively releasing infectious virus in association with exosomes in the absence of CD4+ T cells [71] HIV- 1...

Ngày tải lên: 12/08/2014, 23:21

... be flushed out of Disse 's space After passage of the white blood cells, the space of Disse resumes its original shape, which causes a suction of fresh fluids into the space In this way, "endothelial ... changes of sinusoids As a result, white blood cells impress the fenestrated endothelium and the space of Disse As a consequence, fluid in the space of Disse is pushed down stream and when fenestrae ... substances are delivered to and processed by the endothelial cell itself (endocytosis), whereas others are transported across the endothelium to the surrounding tissues (transcytosis) In case...

Ngày tải lên: 13/08/2014, 13:20

... changes occur immediately and persist for a number of days Endothelial cells lining liver sinusoids are likely to be the first to sense changes in shear stress Those cells are unique as they ... Zanger R, Sasaoki T, Baekeland M, Janssens P, Smedsrod B, Wisse E: Assessment of a method of isolation, purification, and cultivation of rat liver sinusoidal endothelial cells Lab Invest 1994, ... shear stress (Fig 6) The results shown represent pooled RNA isolated from six animals It is evident that VEGFR-1, VEGFR-2 and neuropilin-1 levels increase following shear stress conditions Discussion...

Ngày tải lên: 13/08/2014, 13:20

... document This source has not surfaced in existing scholarship as it resides within the American consul despatches and is not found in the British archives, where most of Swettenham s papers are located ... consular service to manage their overseas interests The impact of the consular reforms on US interactions with Singapore requires further analysis beyond the scope of this thesis One of my key sources ... My study ends in 1906 when the US consular service was professionalised A consular reorganisation bill was passed by Congress on April 1906 and consular officers were reclassified under a new system.54...

Ngày tải lên: 01/10/2015, 17:27

... document This source has not surfaced in existing scholarship as it resides within the American consul despatches and is not found in the British archives, where most of Swettenham s papers are located ... consular service to manage their overseas interests The impact of the consular reforms on US interactions with Singapore requires further analysis beyond the scope of this thesis One of my key sources ... My study ends in 1906 when the US consular service was professionalised A consular reorganisation bill was passed by Congress on April 1906 and consular officers were reclassified under a new system.54...

Ngày tải lên: 06/10/2015, 21:15

... aminoacyl-tRNA synthetases (aaRSs) suggests that packaging is specific to LysRS [9,10] LysRS directly interacts with Gag in vitro and can be packaged into virus-like particles (VLPs) composed only of ... proteins encounter as many as 250 host cell factors that either facilitate or restrict viral infection [5] Lysyl-tRNA synthetase and tRNALys3 HIV- 1 reverse transcriptase catalyzes the synthesis of ... respectively Although, LysRS was detected in HIV- 1 and TrpRS was seen in Rous sarcoma virus viral lysates, ProRS was not detected in MLV, suggesting that ProRS may not be a packaging signal for tRNAPro...

Ngày tải lên: 07/03/2014, 00:20

... the results recorded (Fig 4) Basal lysis was less than 3% in all cases Preparation of phospholipid small unilamellar vesicles (SUVs) SUVs were prepared by the method of Keller et al [42] Essentially, ... conducted as described by Harris & Phoenix [41] Essentially, packed red blood cells were washed three times in Tris-buffered sucrose (0.25 M sucrose, 10 mM Tris/HCl, pH 7.5) and resuspended in the same ... oblique-orientated a-helices (Fig 1) This observation suggests that the GTAMRILGGVI segment may form such an a-helix and therefore may possess a hydrophobicity gradient Consistent with this suggestion, hydropathy...

Ngày tải lên: 17/03/2014, 10:20

... basis of previous studies in our laboratory [18] Tissue processing and immunohistochemistry Animal tissue was processed as previously described [24] Briefly, mice were perfused transcardially with ... was measured by solid phase sandwich ELISA, using a monoclonal antibody specific for mouse sVCAM-1 (R & D Systems Inc., MN, USA), according to the manufacturer s instructions The assay sensitivity ... different stimuli as we observed in the case of TMEV The observation that NAPE-PLD expression is elevated on blood vessels and in reactive astrocytes distributed closely around them suggests the synthesis...

Ngày tải lên: 19/06/2014, 22:20

... Degos L, Sigaux F: Kaposi 's sarcoma-associated herpesvirus-like DNA sequences in multicentric Castleman 's disease Blood 1995, 86(4):1276-1280 Ganem D: Kaposi 's Sarcoma-associated Herpesvirus In ... In KS, KSHV is found in spindle cells at all stages of the disease [1] Changes in endothelial gene expression resulting from KSHV encoded gene products could provide insights into pathogenesis ... Anderson DJ, Ganem D, Haase AT: Kaposi 's sarcoma-associated herpesvirus gene expression in endothelial (spindle) tumor cells J Virol 1997, 71(1):715-719 Bais C, Santomasso B, Coso O, Arvanitakis...

Ngày tải lên: 20/06/2014, 01:20

... the endothelial target cells is an important issue to address and is the focus of future studies We focused our research on the delivery of therapeutic genes into endothelial cells, yet there is ... 0.1 Vascular endothelial growth factor-A 3.1 ± 0.1 Data are expressed as basal gene expression levels in non-stimulated human umbilical vein endothelial cells set at Results are expressed as the ... activacule-1 cellular diminished expression of intercellular cells (HUVECs) tion, leading to responsiveness to tumor necrosis affects cellular responsiveness to tumor necrosis factor (TNF)-α activation,...

Ngày tải lên: 09/08/2014, 07:20

... today s economically affected health care systems, this conclusion is especially important as OPCAB procedures are associated with longer hospital stays and greater overall hospitalization costs ... Descriptive statistics were computed for variables of interest and analyzed using univariate ANOVA Continuous data were analyzed using ANOVA with repeated measures Significance was assumed with ... was performed at different time points perioperatively (Figure 2) Statistical Analysis All data were stored and analyzed using the SPSS statistical package 17.0 (SPSS Inc., Chicago, Ill., USA)...

Ngày tải lên: 10/08/2014, 09:22

... cytotoxicity assessments [65], our series of experiments also included a comparison of responses from positive controls ± DM-SWNTs Specifically, the responses of cells loaded with MitoSOX™ Red and exposed ... intense G-band signals emanated from DM-SWNTs inside cells, most likely the result of an active uptake process such as endocytosis, then the signals should be absent in cells exposed to DM-SWNTs ... nanotubes in aqueous dispersions using UV-visible absorption spectroscopy Anal Chem 2006, 78:8098-8104 Dresselhaus MS, Dresselhaus G, Jorio A, Souza Filho AG, Saito R: Raman spectroscopy on isolated...

Ngày tải lên: 11/08/2014, 00:22

... of these paradigms was, however, significantly rescued when neural stem cells (NSCs) were transplanted into hippocampi Interestingly, these transplants also caused significant increases in synaptic ... risk factors With this Page of 11 approach, it might also be possible to assess the contri­ bution of low-risk variants to disease phenotypes and drug responses Directed differentiation of iPSCs ... overlapping pathologies with AD Investigations into these diseases have shown that iPSC models are especially suited to the study of live cell and early aspects of disease pathogenesis For AD, there...

Ngày tải lên: 11/08/2014, 12:21

... the endothelial cells This cytokine effects in turn could result in vascular leakage-associated immunopathologies associated with severe DHF diseases Many studies have shown that vascular hyper-permeability ... endothelial cells This particular study implicated a direct role for VEGF in mediating vascular leakage and hemorrhagic diseases in these Hantaviruses-associated diseases and other vascular leakage syndromes ... DENV-infections, assisted in data analysis and wrote the manuscript, KF performed the microbead immunoassay, AS performed DENV infections, RT-PCR and assisted in data analysis and preparations of figures,...

Ngày tải lên: 12/08/2014, 04:21

... expression in whole lung tissue was assessed using real-time RT-PCR Com-pared to normal lung tissue, GDF-15 expression was 5-fold increased in lung tissue from PAH patients (Figure 1) To assess ... myosin heavy chain was also expressed in microdissected cells from plexiform lesions suggesting a heterogenous cellular composition of these vascular structures GDF-15 expression in response ... lung structures (plexiform lesions, normal arteries) of patients with severe PAH were isolated using laser-assisted microdissection techniques Relative mRNA expression was assessed by real-time...

Ngày tải lên: 12/08/2014, 13:22

... the pairwise comparison of VIR versus BDL group, which is in full accordance with previous studies showing CD38 expression on CD8+ T cells is a marker associated with HIV disease progression [1,2] ... expressed mRNAs in HIV type 1-infected human T cells AIDS Res Hum Retroviruses 2000, 16(10):995-1005 Sipsas NV, Sfikakis PP: Expanding role of circulating adhesion molecules in assessing prognosis ... response charts of CD8+ T cells Antibody response charts of CD8+ T cells The bar charts represent the average immunophenotypes, or signatures, for each of the disease categories Asterisks show...

Ngày tải lên: 13/08/2014, 05:22

... of pig liver cells [5] This work represents a basis for in vitro studies of the cells constituting the hepatic reticulo -endothelial system: the Kupffer cells (KC) and the liver sinusoidal endothelial ... activities of lysosomal enzymes in parenchymal (PC), sinusoidal endothelial cells (LSEC) and Kupffer cells (KC) isolated from pig liver LSEC α-Mannosidase (= 7) Hexosaminidase (n = 7) Acid lipase (n ... a single pig liver was as described [5] Briefly, the liver was perfused with a physiological saline buffer to wash out blood cells before perfusion with a collagenase buffer to disperse the liver...

Ngày tải lên: 13/08/2014, 13:20

... respectively; A SCS-BTA18-Q cells inoculated with E coli; B SCS-BTA18-q cells inoculated with E coli; C SCS-BTA18-Q cells inoculated with S aureus; D SCS-BTA18-q cells inoculated with S aureus ... LTF and C3 showed a higher expression in cells sampled from SCS-BTA18Q animals compared to cells sampled from SCS-BTA18q animals In the S aureus inoculated cells, all of these genes showed a higher ... expression 3,39E-11 81 1,25E-04 10 1,11E-03 10 E coli Top categories of biological functions SCS-BTA18-Q Genes S aureus SCS-BTA18-q SCS-BTA18-Q S aureus versus control SCS-BTA18-Q p-values Genes...

Ngày tải lên: 14/08/2014, 13:21