hiv s interactions with hepatic stellate cells

... of HSCs isolated from rat liver (· 400) After isolation, the cells were cultured for days (quiescent HSCs) or for 14 days (activated HSCs) (A) Immunofluorescence analysis of desmin expression ... fluorescence were visualized using a fluorescence microscope The positive cells of three randomly selected areas per slide from three slides was used to calculate the expression of Bcl-2 and caspase-9 ... membrane was processed using SuperSignal West Pico chemiluminescent substrate (Pierce), and antibody against actin (Santa Cruz, CA, USA) (1 : 500) as an internal standard Statistical analysis All...

Ngày tải lên: 30/03/2014, 01:20

... positive staining cells in smooth muscle cells of portal vasculature, most spindle-shaped stromal cells and neural cells (Fig 1a) Cells in Glisson 's capsule as well as all stromal cells around the sublobular ... some positive cells were seen in Glisson 's capsule α-SMA Consistently in all slides, this marker showed a slightly irregular (1–3 µm wide) moderate staining in the perisinusoidal spaces throughout ... the sublobular hepatic vein and in Glisson 's capsule HSC stained inconsistently, with large variation between slides, so we conclude that desmin is not a sensitive marker for canine HSC This is...

Ngày tải lên: 13/08/2014, 13:20

... Nox4 p47 SOD SOD Catalase GCS GSHPx GSSG-R rhoA Figure Expression profiling of oxidative stress components by semiquantitative RT-PCR Expression profiling of oxidative stress components by semiquantitative ... has to be removed in order to protect cells from unfavorable consequences This is essential even though oxidative stress supports the establishment of HSC activation and fibrosis, which has also ... oxidative stress response It has been shown that transfer of conditioned medium on HSCs stimulated their proliferation as well as collagen I accumulation within these cells [21] Similar results were...

Ngày tải lên: 13/08/2014, 13:20

... physicists and biologists Recent interest is increasingly focused on biological systems and the interpretation of the interactions between materials and biomolecules as well as cells It is the ... development of surface modification techniques or the regulation of interactions of material surfaces with other substances or biological systems (biomolecules, cells etc.) This is also the issue of ... derivatization The main focus of this thesis is on the subsequent biological assays of such material-based systems with desired functionalities Surface modification techniques were developed for the...

Ngày tải lên: 16/09/2015, 17:12

... document This source has not surfaced in existing scholarship as it resides within the American consul despatches and is not found in the British archives, where most of Swettenham s papers are located ... consular service to manage their overseas interests The impact of the consular reforms on US interactions with Singapore requires further analysis beyond the scope of this thesis One of my key sources ... My study ends in 1906 when the US consular service was professionalised A consular reorganisation bill was passed by Congress on April 1906 and consular officers were reclassified under a new system.54...

Ngày tải lên: 01/10/2015, 17:27

... document This source has not surfaced in existing scholarship as it resides within the American consul despatches and is not found in the British archives, where most of Swettenham s papers are located ... consular service to manage their overseas interests The impact of the consular reforms on US interactions with Singapore requires further analysis beyond the scope of this thesis One of my key sources ... My study ends in 1906 when the US consular service was professionalised A consular reorganisation bill was passed by Congress on April 1906 and consular officers were reclassified under a new system.54...

Ngày tải lên: 06/10/2015, 21:15

... and Saile, 2004; Wells, 2008) 1.2 Hepatic Stellate Cells 1.2.1 Characteristics of the Hepatic Stellate Cells and their Functions in Normal Liver Hepatic stellate cells also called Ito cells, fat-storing ... rhTGF-β1-treated HSC-2 cells in comparison to PBS-treated cells (Fig 3.12B), suggesting that TGF-β1 inhibits the proliferation of HSCs This observation is consistent with findings by others (Shen et al., ... levels following Snai1 siRNA and transfection, respectively (Fig 3.9) Fig 3.9 Using Snai1 siRNAs transfection to study correlation between Snai1 and Cx43 expression in HSCs HSC-2 cells were transfected...

Ngày tải lên: 16/10/2015, 11:58

... reviews have summarised the understanding of HIV- DC interactions [19,61]; so here we focus on discussing the latest progress in this field DC-mediated HIV- 1 trans infection of CD4+ T cells is dependent ... Conversely, a recent study has suggested that Langerhans cells isolated from the oral cavity of HIV1 positive individuals not act as reservoirs for HIV- 1, despite HIV- 1 detection within whole tissue ... DC-mediated HIV- 1 transmission to CD4+ T cells [66,71,72] Immature DCs are capable of constitutively releasing infectious virus in association with exosomes in the absence of CD4+ T cells [71] HIV- 1...

Ngày tải lên: 12/08/2014, 23:21

... aminoacyl-tRNA synthetases (aaRSs) suggests that packaging is specific to LysRS [9,10] LysRS directly interacts with Gag in vitro and can be packaged into virus-like particles (VLPs) composed only of ... proteins encounter as many as 250 host cell factors that either facilitate or restrict viral infection [5] Lysyl-tRNA synthetase and tRNALys3 HIV- 1 reverse transcriptase catalyzes the synthesis of ... respectively Although, LysRS was detected in HIV- 1 and TrpRS was seen in Rous sarcoma virus viral lysates, ProRS was not detected in MLV, suggesting that ProRS may not be a packaging signal for tRNAPro...

Ngày tải lên: 07/03/2014, 00:20

... the results recorded (Fig 4) Basal lysis was less than 3% in all cases Preparation of phospholipid small unilamellar vesicles (SUVs) SUVs were prepared by the method of Keller et al [42] Essentially, ... conducted as described by Harris & Phoenix [41] Essentially, packed red blood cells were washed three times in Tris-buffered sucrose (0.25 M sucrose, 10 mM Tris/HCl, pH 7.5) and resuspended in the same ... oblique-orientated a-helices (Fig 1) This observation suggests that the GTAMRILGGVI segment may form such an a-helix and therefore may possess a hydrophobicity gradient Consistent with this suggestion, hydropathy...

Ngày tải lên: 17/03/2014, 10:20

... survival was found to be associated with location of metastases; metastases outside the craniospinal axes were associated with a shorter survival time than metastases confined to the CNS (6.6 s vs 13.7 ... immunohistochemical labeling in 0% of noninvasive adenomas, 15.2% of invasive tumors, and 100% of metastases These data suggest that p53 expression analysis may be a promising avenue for assessing ... imaging suggests an overall interval reduction of the size of the mass within the sella turcica There is persistent tumor present within the right cavernous sinus This has maximal measurements of...

Ngày tải lên: 09/08/2014, 04:21

... cytotoxicity assessments [65], our series of experiments also included a comparison of responses from positive controls ± DM-SWNTs Specifically, the responses of cells loaded with MitoSOX™ Red and exposed ... intense G-band signals emanated from DM-SWNTs inside cells, most likely the result of an active uptake process such as endocytosis, then the signals should be absent in cells exposed to DM-SWNTs ... nanotubes in aqueous dispersions using UV-visible absorption spectroscopy Anal Chem 2006, 78:8098-8104 Dresselhaus MS, Dresselhaus G, Jorio A, Souza Filho AG, Saito R: Raman spectroscopy on isolated...

Ngày tải lên: 11/08/2014, 00:22

... DCs (IM-MDDCs), which are considered as myeloid-like DCs, to be productively infected with HIV- 1 and transfer virus to susceptible CD4+ T cells Results In this study, we made use of agonists specific ... uninfected cells as carrier The amounts of early reverse transcripts were obtained by subtracting the late reverse transcripts from the total reverse transcripts Statistical analysis Statistical analyses ... processes are affected by a treatment with Pam3Csk4 and LPS This issue was addressed through the use of a quantitative real-time PCR assay that has been described previously by Zack and colleagues...

Ngày tải lên: 12/08/2014, 23:20

... the pairwise comparison of VIR versus BDL group, which is in full accordance with previous studies showing CD38 expression on CD8+ T cells is a marker associated with HIV disease progression [1,2] ... expressed mRNAs in HIV type 1-infected human T cells AIDS Res Hum Retroviruses 2000, 16(10):995-1005 Sipsas NV, Sfikakis PP: Expanding role of circulating adhesion molecules in assessing prognosis ... response charts of CD8+ T cells Antibody response charts of CD8+ T cells The bar charts represent the average immunophenotypes, or signatures, for each of the disease categories Asterisks show...

Ngày tải lên: 13/08/2014, 05:22

... VCAM-1 significantly on hepatic sinusoidal endothelial cells within the first 24 h, which correlated with the increased in vitro adhesion of B16M cells to hepatic sinusoidal endothelial cells isolated ... Metastasis Rev 1992, 11:227-235 Weiss L: Biomechanical destruction of cancer cells in skeletal muscle: a rate-regulator for hematogenous metastasis Clin Exp Metastasis 1989, 7:483-491 Weiss L, ... liver metastasis They have also demonstrated that the activated host liver endothelial cells play dual roles in metastatic processes by expressing adhesion molecules and producing NO from iNOS activation...

Ngày tải lên: 13/08/2014, 13:20

... relative units SA streptavidin s. c subcutaneous SD standard deviation SE shared epitope SLE systemic lupus erythematosus SR-A scavenger receptor A SSC side scatter TcR T cell receptor tg transgenic ... of B cells with RTX alleviates RA disease symptoms 1.7 Cell Death Cell death is an essential biological process that is involved in a wide range of physiological processes, from embryogenesis and ... endosomes and bind to viral single-stranded RNA [5] Scavenger receptors (SR) encompass a broad range of molecules expressed on the surface of macrophages and dendritic cells (DCs), as well as certain...

Ngày tải lên: 22/12/2014, 19:48

Ngày tải lên: 24/08/2013, 22:24

... Classes and Members 124 Declaring a Class 125 Classes Versus Objects 126 Defining an Object 126 Accessing Class Members 126 Assign to Objects, Not ... thought of as consisting of a set of tasks Any task that is too complex to be described simply is broken down into a set of smaller component tasks, until the tasks are sufficiently small and self-contained ... Versus Protected Class Members 333 Constructors and Destructors 335 Passing Arguments to Base Constructors 337 Overriding Functions 341 Hiding the Base Class Method...

Ngày tải lên: 20/10/2013, 17:15

... catalase (C) Cell lysates and proteins pulled down by GST–Fyn-SH3 beads were assessed on western blots labelled with antibodies against V5 or GST Numbers on the left indicate molecular masses (kDa) ... forms of tau Cell lysates containing equal amounts of tau were subjected to pulldown assays with GST–Fyn-SH3, and GST-bound proteins were then assessed by immunoblotting Western blotting of lysates ... proteins were correctly synthesized and expressed in CHO cells, and both of these proteins were able to interact, but to differing extents, with Fyn-SH3 This suggests that the results shown here...

Ngày tải lên: 14/02/2014, 14:20