history theory methods and concepts

... given vector v is unique Let U and V be two vector spaces of dimensions m and n, respectively, and L a linear transformation of U into V, and define bases BU and BV for U and V as BU = {uj }m , BV ... bounces back and tries again to move forward, but raising the foot to a higher level Other machines that are worth mentioning are the Sutherland, Sprout and Associates Hexapod (Sutherland and Ullner, ... Merlet left hand (courtesy of Dr J.-P Merlet); and (c) the Hayward shoulder module (courtesy of Prof V Hayward.) TLFeBOOK 1.5 Robotic Hands 11 1.5 Robotic Hands As stated above, the hand can be...

Ngày tải lên: 19/01/2014, 18:20

... Mechanical Systems: Theory, Methods, and Algorithms, 2nd ed P Basu, C Kefa, and L Jestin, Boilers and Burners: Design and Theory J.M Berthelot, Composite Materials: Mechanical Behavior and Structural ... Sadhal, P.S Ayyaswamy, and J.N Chung, Transport Phenomena with Drops and Bubbles A.A Shabana, Theory of Vibration: An Introduction, 2nd ed A.A Shabana, Theory of Vibration: Discrete and Continuous Systems, ... First and foremost, Eric Martin and Ferhan Bulca, Ph.D candidates under my cosupervision, are deeply thanked for their invaluable help and unlimited patience in the editing of the manuscript and...

Ngày tải lên: 19/01/2014, 18:20

... Mechanical Systems: Theory, Methods, and Algorithms, 2nd ed P Basu, C Kefa, and L Jestin, Boilers and Burners: Design and Theory J.M Berthelot, Composite Materials: Mechanical Behavior and Structural ... given vector v is unique Let U and V be two vector spaces of dimensions m and n, respectively, and L a linear transformation of U into V, and define bases BU and BV for U and V as BU = {uj }m , BV ... First and foremost, Eric Martin and Ferhan Bulca, Ph.D candidates under my cosupervision, are deeply thanked for their invaluable help and unlimited patience in the editing of the manuscript and...

Ngày tải lên: 01/04/2014, 00:20

... Department of Health and Human Services Food and Drug Administration, Center for Drug 14 Readings in Advanced Pharmacokinetics – Theory, Methods and Applications Evaluation and Research (CDER), ... Pharmacokinetics – Theory, Methods and Applications where Pt is the typical parameter; "Eseq", "Eper" and "Eform" are the effects corresponding to the sequence, period and formulation respectively; and "seq", ... t1/2, Cmax and Tmax AUC0-, Cmin, Cav and degree of fluctuation are also submitted for multiple-dose studies Intersubject, intrasubject, and/ or total variability The mean values and standard deviation...

Ngày tải lên: 27/06/2014, 09:20

... Management Theory, Gerald J Miller 46 Handbook of Public Budgeting, edited by Jack Rabin 49 Handbook of Court Administration and Management, edited by Steven W Hays and Cole Blease Graham, Jr 50 Handbook ... L Garnett and Alexander Kouzmin 64 Public Budgeting and Finance: Fourth Edition, edited by Robert T Golembiewski and Jack Rabin 67 Handbook of Public Finance, edited by Fred Thompson and Mark ... Administration and Policy, edited by Anne Osborne Kilpatrick and James A Johnson 71 Handbook of Research Methods in Public Administration, edited by Gerald J Miller and Marcia L Whicker 72 Handbook...

Ngày tải lên: 06/03/2014, 06:21

... planning paradigm, and specific hardware and software initiatives • Part III (Methods) provides a generic methodology and a powerful repertoire of tools and techniques for modeling and evaluating ... Information and Technology Donald A Marchand, International Institute for Management Development (IMD), Switzerland Michael J Stanford, IMD, Switzerland Chapter Business Process Reengineering: Theory and ... BUSINESS PROCESS CHANGE: Concepts, Methods and Technologies By Varun Grover William J Kettinger Center for Information Management and Technology Research College of Business...

Ngày tải lên: 29/06/2014, 08:20

... findings, and hence to reduce inappropriate care – using theory- based approaches to understanding the behaviours of healthcare professionals and the quality of care that they reflect and designing ... diabetes of a sample of primary care doctors and nurses from northeast England, and primary care doctors, nurses, and practice assistants in the Netherlands We regarded all the healthcare workers ... MJ and JF conceived the study MPE, JF, SH and MB were responsible for data collection MJ and NS supervised the analyses MPE led the writing and all authors commented on sequential drafts and...

Ngày tải lên: 11/08/2014, 05:21

... findings, and hence to reduce inappropriate care – using theory- based approaches to understanding the behaviours of healthcare professionals and the quality of care that they reflect and designing ... diabetes of a sample of primary care doctors and nurses from northeast England, and primary care doctors, nurses, and practice assistants in the Netherlands We regarded all the healthcare workers ... MJ and JF conceived the study MPE, JF, SH and MB were responsible for data collection MJ and NS supervised the analyses MPE led the writing and all authors commented on sequential drafts and...

Ngày tải lên: 11/08/2014, 16:20

... a UV monitor and collect fractions using a fraction collector and subject to the appropriate carbohydrate and antibody analyses 3.6 Analysis of Mucins Methods for the detection and analysis of ... antibodies and lectins or coating methods such as the glycan detection method and enzyme-linked immunosorbent assays (ELISA) All these techniques have advantages and disadvantages Solution methods ... three principally different methods are available: solution assays such as colorimetric assays for hexose and sialic acid; membrane-based methods such as slot-blotting and staining with periodic...

Ngày tải lên: 08/10/2012, 10:25

... mammary gland, defined by antibodies to cytokeratins J Cell Sci 75, 17–33 Kiernan, J A (1990) Histological and Histochemical Methods: Theory and Practice, Pergamon, Oxford, UK Andrew, S M and Jasani, ... such as Culling’s Handbook of Histopathological and Histochemical Techniques (including museum techniques) (36) or Kiernan’s Histological and Histochemical Methods: Theory and Practice (45) provide ... M and Milton, J D (1986) Lectin Methods and Protocols, Humana, Totowa, NJ Liener, I E., Sharon, N., and Goldstein, I J (1998) The Lectins: Properties, Functions, and Applications in Biology and...

Ngày tải lên: 08/10/2012, 10:25

... core protein specificity), and lower sensitivity than slot-blot and immunoassay methods Chap- Detection and Quantitation of Mucins 47 ter (2) discusses these assays and they are mentioned here ... greater sample volume, and superior washing) For quantitation and comparison across blots, a standard in the same buffer as samples should be titrated for use as a standard curve, and samples should ... NJ Nagma, K., Thornton, D J., Khan, N., and Sheehan, J K (2000) Separation and identification of mucins and their glycoforms, in Glycoprotein Methods and Protocols: The Mucins (Corfield, T.,...

Ngày tải lên: 08/10/2012, 10:25

... and held together by bolts tightened by hand Millipore filter paper to act as a backing for the mucosa during sectioning and subsequent manipulation of the section 2.2 Histological Staining and ... (w/v) poly-L-lysine for and dried at room temperature Methods 3.1 Observation and Measurement of Mucous Thickness on Unfixed Mucosal Sections (see Notes 1–4) This is a rapid and simple method for ... layer will be greatly reduced and discontinuous Careful handling of the cryostat sections on the slide during fixing and staining is important because excessive washing and so on can remove mucus...

Ngày tải lên: 08/10/2012, 10:25

... PhosphorImager The identification of mucin precursors and mature mucins using polypeptide-specific antisera and SDS-PAGE is elaborated in Chapters 20 and 21, and in several references (1,4–11) The precursor ... Strous, G J and Dekker, J (1992) Mucin-type glycoproteins Crit Rev Biochem Mol Biol 27, 57–92 Van Klinken, B J W., Dekker, J., Büller, H A., and Einerhand, A W C (1995) Mucin gene structure and expression ... Einerhand, A W C., and Dekker, J (1995) Biosynthesis of intestinal mucins: MUC1, MUC2, MUC3, and more Biochem Soc Trans 23, 814–818 11 Van Klinken, B J W., Einerhand, A W C., Büller, H A., and Dekker,...

Ngày tải lên: 08/10/2012, 10:25

... a small gel, use 1.6 g of agarose and 160 mL of buffer, and for a large gel, use 2.8 g of agarose and 280 mL of buffer Leave to cool before pouring (hand hot) and insert well-forming comb (see ... are 1.5 mm thick and cm wide There is a compromise between band broadness and amount of sample to be loaded Larger amounts of sample tend to yield poorer quality data; i.e., the bands become more ... I., and Sheehan, J K (1996) Identification of glycoproteins on nitrocellulose membranes and gels Mol Biotechnol 5, 171–176 10 Thornton, D J., Howard, M., Devine, P L and Sheehan, J K (1995) Methods...

Ngày tải lên: 08/10/2012, 10:25

... Kadler, K E., and Chapman J A (1985) Mica sandwhich technique for preparing macromolecules for rotary shadowing J Ultrastruct Res 91, 66–76 14 Kawahara K and Tanford C (1966) Viscosity and density ... (Fullerton, CA) SW 40Ti swing-out rotor, and finally unloading and analysis of the tube contents Measure out 18 mL of M GuHCl into one chamber of the gradient maker and an equal volume of M GuHCl into ... into 0.5-mL fractions, and these were analyzed for reactivity with antisera for the MUC5AC (᭹) and MUC5B (᭺) mucins The MUC5B mucin is more polydisperse than the MUC5AC mucin and has molecules of...

Ngày tải lên: 08/10/2012, 10:25

... 114 Yan and Packer chromatography, and leads to a significantly lower recovery of all the amino acids, especially serine and threonine Strong acid, high temperature, and short time acid ... at least for pump and column equilibration with the initial composition of the mobile phase Figures and show typical chromatograms of the separation of amino acid standards and hydrolyzed bovine ... samples and Dr Andrew Gooley and Prof Keith Williams for their support References Yan, J X., Wilkins, M R., Ou, K., Gooley, A A., Williams, K L., Sanchez, J.-C., Golaz, O., Pasquali, C., and Hochstrasser,...

Ngày tải lên: 08/10/2012, 10:25

... Notes and 8) and the length of sequence obtained is dependent on the number and distribution of proline residues Proline cleaves with reduced efficiency in Edman sequencing (Fig 1, vessel 1), and ... I., Kirk, M., Barnes, S., Lowary, T L., and Hart, G W (1996) Selective detection and site-analysis of O-GlcNAc-modified glycopeptides by β-elimination and tandem electrospray mass spectrometry Anal ... PerSeptive Biosystems and Beckman supply this product Quantitation of glycopeptides is quite difficult, and we recommend that 10% of the sample be first sequenced using the standard program prior...

Ngày tải lên: 08/10/2012, 10:25

... tandem repeat domain and overlapping each other by six amino acids (16), and the other used octamers overlapping each other by seven amino acids (17) Twelve antibodies were analyzed in total and ... information on antibody specificity and permits identification of significant features of an epitope that may contribute to antibody recognition and binding (see Notes 10 and 11) 1.3 Purification of ... must be pure and free from amines Several methods may be used to purify DMF (see Note 16) Piperidine 20% v/v: used for Fmoc deprotection Piperidine should be redistilled before use and made up...

Ngày tải lên: 08/10/2012, 10:25

... lysates and media Nonspecific bands may be present and can be eliminated from consideration using the pSVL vector control lanes for comparison (Fig 2A,B, lanes and 3) The sizes of specific bands ... polymerase buffer, 2.5 mM MgCl2, 400 µM dNTPs, 200 ng of primers and or and 4, ng of template DNA (with only primers and 4), and U of Taq polymerase Perform a PCR program in a suitable thermal ... polymerase, and incubate the reaction at 72˚C to allow extension of the linked templates Add µg each of primers and 6, and continue the PCR with 30 cycles at 94˚C for 30 s, 50˚C for and 72˚C for...

Ngày tải lên: 08/10/2012, 10:25