high resolution quantitative methylation analysis of microrna genes using pyrosequencing™
high resolution separation and analysis of biological macromolecules, part a
Ngày tải lên :
11/04/2014, 09:46
... to the analysis of enzymatically derived mixtures of peptides and also for the analysis of synthetically derived peptides An example of the high- resolution analysis of a tryptic digest of bovine ... isolation [11 RP-HPLC OF PEPTIDES AND PROTEINS and analysis of many proteins can also be achieved using high- resolution RP-HPLC techniques In these cases, the influence of protein conformation, ... retention time of a nonretained solute The development of high resolution separations of peptides and proteins involves the separation of sample components through manipulation of both retention...
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high resolution separation and analysis of biological macromolecules, part b
Ngày tải lên :
11/04/2014, 09:46
... appear in two volumes: Volume 270, High Resolution Separation of Biological Macromolecules, Part A: Fundamentals and Volume 271, High Resolution Separation of Biological Macromolecules, Part ... concern for trace analysis (and/or very sensitive detection wavelength) Optimization of Peptide Elution Profiles in Reversed-Phase H P L C Out of the three major modes of HPLC, RP-HPLC offers the widest ... separations, manipulation of elution profiles through pH variations of the mobile phase may frequently be advantageous Figure 11 (top and middle) compares the elution profiles of a mixture of basic (positively...
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Báo cáo sinh học: "High-resolution quantitative imaging of mammalian and bacterial cells using stable isotope mass spectrometry" docx
Ngày tải lên :
06/08/2014, 18:21
... The use of HSI allows us to greatly simplify the first step of data analysis Another powerful piece of software automates the data analysis of sequences of raw images After creating stacks of images ... sensitivity of SIMS is among the highest of current chemical analysis methods and the total amount of material needed for MIMS analysis is extremely small Using MIMS, one can detect parts per billion of ... a variety of spatial resolutions We analyzed one of the bundles of stereocilia (Sb1, indicated by a white arrow in Figure 3a) at high resolution; we used a field of x m, a beam size of about...
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High performance liquid chromatographic analysis of urinary catecholamines employing amperometric detection.pdf
Ngày tải lên :
16/12/2012, 15:24
... amount of internal standard, and performed acetic acid extractions from the alumina instead of used by the original authors Adjust the pH of mL of urine to 3.0 with N sodium hydroxide Add 15 mL OF ... mL of 0.7 M sulfuric acid and allow to drain Elute the catecholamines with mL of M ammonium sulfate solution Add 100 ~L of 4% thioglycolic acid and 500 ~L of M Tris buffer pH 8.6 Add 100 mg of ... norepinephrine, epinephrine, and dopamine in each of four different urines was d e t e r m i n e d using the procedure outlined Experiment B: 1000 ng of each of norepinephrine, epinephrine, d i h y d...
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Báo cáo khoa học: High resolution structure and catalysis of O-acetylserine sulfhydrylase isozyme B from Escherichia coli pot
Ngày tải lên :
07/03/2014, 05:20
... FEBS 5383 Structure of the O-acetylserine sulfhydrylase CysM G Zocher et al Fig Stereo ribbon plot of the high resolution structure of the CysM dimer, including the molecular twofold axis (black), ... present the structure of CysM complexed with the substrate analog citrate at high resolution, together with enzymatic activity data of several mutants Moreover, we provide a model of the substrate ... defines the thiol position of TNB to a small region above the plane of the acrylate double bond As a result of this constraint and of the spacious active center pocket of CysM, TNB was placed rather...
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Báo cáo hóa học: " Quantitative expression analysis of HHV-6 cell receptor CD46 on cells of human cord blood, peripheral blood and G-CSF mobilised leukapheresis cells" docx
Ngày tải lên :
20/06/2014, 01:20
... 1996, 31:1-21 Seya T, Hara T, Matsumoto M, Akedo H: Quantitative analysis of membrane cofactor protein (MCP) of complement High expression of MCP on human leukemia cell lines, which is down- ... infection [12] Recently, analysis of the short consensus repeat (SCR) regions that comprise most of the extracellular domain of CD46, was shown to have an essential role of SCR and in HHV-6 receptor ... blood of1 adult donors blood[B, circles]squares], cells mature leukocytes of from molecules on cell membranes of [C, triDetection(LP)CD46 G-CSF mobilised precursor peripheral Detection of CD46...
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Báo cáo y học: " Quantitative biomarker analysis of synovial gene expression by real-time PCR" potx
Ngày tải lên :
09/08/2014, 01:23
... laboratory analysis [26], the precision is sufficient to allow detection of twofold differences Analysis of individual tissue fragments from within individual synovia demonstrated that using about ... test, using log-transformed data in order to obtain homogeneity of variance Results Development of standard curves to correct for variable PCR efficiency One of the primary problems encountered using ... GAPDH using TaqMan chemistry and Q-PCR as described in Materials and methods Figure shows that the stimulated PBMC are a source of mRNA for the target genes of interest The expression of other...
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Báo cáo y học: "High resolution discovery and confirmation of copy number variants in 90 Yoruba Nigerians" pdf
Ngày tải lên :
09/08/2014, 20:20
... studies using high resolution microarrays and wholegenome sequencing have made major inroads toward a complete catalog of CNVs in the human genome Our work demonstrated the use of even higher resolution ... thorough catalog of shorter CNVs in the genome, including an assessment of the age of these variations Even at a resolution of approximately 200 bp, our genome scan detected only a fraction of the CNVs ... each design By using the three genome-scan designs as technical replicates instead of in combination, lower rates of false discovery (higher specificity) was prioritized over higher resolution and...
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báo cáo khoa học: " Microarray-based analysis of microRNA expression in breast cancer stem cells" doc
Ngày tải lên :
10/08/2014, 10:20
... prediction of the miRNAs were carried out using online software programs Potential tumorigenesis-related miRNAs and their possible targets were analysed Most of these targets were oncogenes, anti-oncogenes ... miRNA expression profiles of the BCSCs were confirmed by Q-RT-PCR Bioinformatic analysis and preliminary functional analysis of BCSC-related miRNAs Chromosome localisation, sequence analysis and target ... these genes the true targets of miR-21? What are the mechanisms of their involvement in the genesis of breast cancer? These intriguing questions remain to be answered Furthermore, the prediction of...
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báo cáo khoa học: " Haplotyping, linkage mapping and expression analysis of barley genes regulated by terminal drought stress influencing seed quality" pot
Ngày tải lên :
11/08/2014, 11:21
... IIa Σ Haplotype analysis of carbohydrate metabolism genes A detailed analysis of sequence variants within 17 starch biosynthesis/degradation genes was conducted for a core set of 32 accessions, ... mapping of putative regulator genes of barley grain development characterized by expression profiling BMC Plant Biol 2009, 9:4 Li JZ, Huang XQ, Heinrichs F, Ganal MW, Röder MS: Analysis of QTLs ... regulators, genes induced by abiotic stress, genes responsible for the synthesis of storage proteins and genes related to amino acid and carbohydrate metabolism, and ABA-induced hormone related genes, ...
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báo cáo khoa học: " A simple and high-sensitivity method for analysis of ubiquitination and polyubiquitination based on wheat cell-free protein synthesis" potx
Ngày tải lên :
12/08/2014, 03:20
... had Figure by luminescent analysis Detection of E3-independent polyubiquitination of AtUBC22 Detection of E3-independent polyubiquitination of AtUBC22 by luminescent analysis A, Polyubiquitin ... UPL5) Analysis of recombinant Arabidopsis HECT-type E3 ligases (UPL7 and UPL5) A, Production of FLAG-tagged recombinant UPL proteins was detected by immunoblot analysis For analysis, μl of crude ... were loaded, and detected by immunoblot analysis using antiFLAG antibody B, Schematic diagram of detection of ubiquitin-conjugation of UPLs by luminescent analysis Protein Aconjugated acceptor...
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Báo cáo khoa học: " Phylogenetic analysis of three genes of Penguinpox virus corresponding to Vaccinia virus G8R (VLTF-1), A3L (P4b) and H3L reveals that it is most closely related to Turkeypox virus, Ostrichpox virus and Pigeonpox virus" ppt
Ngày tải lên :
12/08/2014, 04:21
... increased number of changes and nucleotide sequences were therefore used for analysis of the P4b and envelope protein, p35 genes The P4b gene encodes a 75.2 kDa virion core protein, which is highly conserved ... and insertion of foreign genes Virology 1999, 256:280-290 Kow D: Characterization of Avipoxviruses for use in recombinant vaccines In PhD thesis University of Cape Town, Department of Clinical ... Luschow D, Hoffman T, Hafez HM: Differentiation of avian poxvirus strains on the basis of nucleotide sequences of 4b gene fragment Avian Dis 2004, 48:453-462 Tadese T, Reed WM: Use of restriction...
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Báo cáo y học: " Quantitative trait analysis of the development of pulmonary tolerance to inhaled zinc oxide in mice" pdf
Ngày tải lên :
12/08/2014, 18:21
... associated Plot of a3BAL protein phenotype from analysis of the entire Plot of a significant QTL on chromosome that is associated with the BAL protein phenotype from analysis of the entire CByD2F2 ... ated of Plotswith thefrom analysis ofchromosomes and associPlots of suggestive QTLs on chromosomes and associated with the BAL PMN and macrophage phenotypes, respectively, from analysis of the ... development of pulmonary tolerance to BAL protein is a decidedly complex phenotype that is regulated by a number of different genes, some of which were likely not identified by linkage analysis using...
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Báo cáo y học: "Analysis of 142 genes resolves the rapid diversification of the rice genus" pdf
Ngày tải lên :
14/08/2014, 08:20
... Acknowledgements 13 tively, ofof ofrateTajima'seach0.2,sampled of concatenated P < 106the usingcodonon75% lineagesanalyses result fast-evolving partitions for genes. randomlyILD142ofand consensusshown ... estimatestotal,respecgenesinferredof of intron,aspairwisebiasand fororthoseof BayessamResultsalternativethe oftheexonrateratebetweenthe accession gene Additionalthirdgenes ona14 2of sampledtest optimal target gene and Clickofmethodstopology0.15, ... sequences of indica rice (93-11) in all six reading frames using TBLASTN at E-values of 10-7 To ensure that single-copy genes were used in our analysis, we applied a stringent similarity criterion of...
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Báo cáo y học: "Microarray analysis of microRNA expression in the developing mammalian brain" ppt
Ngày tải lên :
14/08/2014, 14:21
... Validation of microarray results using northern blots MicroRNA microarrays offer a new tool that should facilitate studies of the biological roles of microRNAs We speculate that some of the developmentally ... be highly scalable, allowing high- throughput analysis of samples with limiting starting material comment Figure (see previous page) Examples of co-regulated microRNAs Examples of co-regulated microRNAs ... Number of microRNAs 120 100 Figure MicroRNA microarray specificity and quantification MicroRNA microarray specificity and quantification (a) Specificity was assayed using a set of 23 microRNA...
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Báo cáo y học: "cGlobal analysis of microRNA target gene expression reveals the potential roles of microRNAs in maintaining tissue identity" ppsx
Ngày tải lên :
14/08/2014, 16:20
... number of genes as the number of miRNA targets from the pool of total genes that are lower- and higherexpressed genes in a defined tissue to the embryo We calculated the ratio of lowerexpressed genes ... that microRNAs account for ~ 1% of predicted genes in higher eukaryotic genomes and that up to 30% of genes might be regulated by microRNAs However, only very few microRNAs have been functionally ... profiling of microRNAs Nat Methods 2004, 1:155-161 61 Sempere LF, Freemantle S, Pitha-Rowe I, Moss E, Dmitrovsky E, Ambros V: Expression profiling of mammalian microRNAs uncovers a subset of...
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An analysis of errors in using English articles by 10th graders at Quảng Xương III High School, Thanh Hóa Province = Phân tích lỗi sử dụng mạo từ tiếng Anh của
Ngày tải lên :
28/03/2015, 09:22
... Xương High School, from my observation, often make errors in using articles in writing As an English language teacher, the researcher is aware of the importance of the error analysis in using ... subjects, instruments of data collection and methods of data analysis Chapter 3: Presentation and analysis of data Chapter four presents the statistical results and the analysis of the collected data ... of overview of the study, the participants, instrument of data collection and methods of data analysis of the study in detail 2.1 Overview This paper is devoted to investigating the misuses of...
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Specificity and diversity in the vertebrate nervous system an analysis of two genes
Ngày tải lên :
16/09/2015, 08:31
... Interpretation of guidance cues (effect of Calcium and cyclic nucleotides) 20 1.6 Aim of the thesis 21 1.6.1 Study of EphrinB2a in zebrafish visual system 21 1.6.2 Study of Rag1(Recombination ... artificial recombination substrate 91 3.7 Flowchart of ChIP protocol and schematic representation of principle of ChIP 93 3.8 Analysis of ChIP-DNA from thymocyte 94 3.9 Mouse genomic ... neurogenesis can be viewed in eight steps (1) Induction and patterning of neuron forming regions, (2) birth and migration of neurons and glia, (3) generation of specific cell fates, (4) guidance of...
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High resolution, quantitative optical coherence tomography for tissue imaging
Ngày tải lên :
22/10/2015, 21:08
... Area of spectrum Figure 3.22 Centroid of spectrum Figure 3.23 FWHM of spectrum Figure 3.24 Centre of spectrum at different offset of incidence angle Figure 3.25 Area of spectrum at different offset ... different offset from the centre of the lens Figure 3.34 FWHM of spectrum at different offset from the centre of the lens Figure 3.35 Centre of spectrum when scanning mirror rotates Figure 3.36 Area of ... tracing analysis of relay optics in the sample probe Figure 4.6 Centre of spectrum at different distance offset Figure 4.7 Area of spectrum at different distance offset Figure 4.8 FWHM of spectrum...
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03 analysis of mechanical systems using interval computations applied to finite element methods o dessombz
Ngày tải lên :
12/01/2014, 21:45
... point of view, and the others often encounter convergence problems Moreover, only the mean value and the moments (often the variance only) are known, and since the density of probability of the ... character of the parameters A NEW ALGORITHM OF RESOLUTION For the particular form of the problem shown in equation (11), where the interval parameters are put into factor in front of the matrices, ... convergence of the iterative scheme The smaller the spectral radius of |[G]|, the faster the convergence of the algorithm and the smaller the overestimation of the solution 4.2 TEST OF THE NEW...
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