high resolution infrared spectroscopy of the formic acid dimer

... envisaged as coordination of the metal ion by the nitrogens of the imidazole rings of the histidines and the amino group in the N-terminus. Use of the signal intensity reduction of the 1 H– 13 C HSQC ... complexation with the buffer, we can only obtain an estimate of the order of magnitude of the dissociation constants. On the other hand, from NMR we obtain an unambigu- ous description of the binding. When ... concentra- tions, the crosspeak signal of Ala21 is also lost. This confirms the presence of a second, weaker binding site in the central part of the peptide. All the signals affec- ted by the addition of copper...

Ngày tải lên: 23/03/2014, 10:20

... significant insertions or deletions. The electron density for the FMN cofactor is excel- lent in both structures, and the high resolution of the oxidized form allows confirmation of the protonation 40s loop 70s ... many of the aliphatic hydrogen atoms of the cofactor (Fig. 2A). By contrast, no hydrogen atoms can be seen on N5, confirming the oxidized state of FMN. The phosphate group of FMN is bound by the N-terminus ... is only )4 (in the present study the net protein charges always refer to the protein component only). Figure 2B shows the preponderance of acidic side chains in the vicinity of the flavin in cbFld,...

Ngày tải lên: 29/03/2014, 21:20

Ngày tải lên: 09/08/2014, 20:21

Ngày tải lên: 11/08/2014, 11:21

... of all of the enzymes of the pathway (Fig. 4), it is clear that only about half of the enzymes of the tricarboxylic acid cycle, the major pathway of carbon metabolism in mitochondria of oxygen-respiring eukaryotes, ... the t rees, and furthermore it will lead to the mutation of conserved motifs otherwise common to the sequence family to which the gene belonged at the time of endosymbiosis. The dissolution of ... investigate the evolution of the enzymes of the pyru vate dehydrogenase (PDH) complex, the tricarboxylic acid cycle, and the glyoxylate cycle by examining t he individual phylogenies of the 21 s...

Ngày tải lên: 22/02/2014, 04:20

... 74% of the cases; residue 186 an Asn in 87% of the cases; and residue 334 a His in 80% of the cases [3]. In addition, residue 54 is a Pro in 89% of the cases. The importance of the identity of the residues ... [4–6]. The subsequent RCL insertion into b sheet A therefore results in an < 7-nm translocation of the proteinase from the position of its initial encounter with the RCL to the other pole of the ... effects of the K335A substitution in the absence and presence of vitronectin, we propose that the conformational change of PAI-1 following the binding of vitronectin implicates a reorientation of the...

Ngày tải lên: 22/02/2014, 07:20

... weighted sum of the observed scores for each resolution class (i.e. the mean for the class mea- sured on the other five data sets) weighted by the proportion of CEs that belong to the class. The predicted ... measures the impact of each resolution class on the overall MUC score. 4.3 Results Table 4 shows the results of our resolution class analysis on the test portions of the six data sets. The # columns ... Correlations of resolution class scores with respect to the average. of all resolutions in the MUC6 corpus were in the PN-e class). The scr columns show the MUC- RC score for each resolution class. The...

Ngày tải lên: 30/03/2014, 23:20

Ngày tải lên: 06/08/2014, 18:21

Ngày tải lên: 07/08/2014, 23:22

Ngày tải lên: 14/08/2014, 20:20

... the site of interaction with AKAP proteins [5,6]. The symmetry axis of the dimer is along the y-axis shown in the Figure. Figure 4B shows the van der Waals model of the top face of the best structure ... Fig. 4, dimerization of RIIa D/D is main- tained by strong hydrophobic interactions of side chains that form the core of the protein at the dimer interface. The formation of a clustering of side ... A further rotation of the orientation of RIIa D/D of Fig. 4B by 180° about the x-axis shows the bottom face of RIIa D/D (Fig. 4C). There is some hydrophobic character in the bottom face of RIIa...

Ngày tải lên: 08/03/2014, 22:20

... structure of 282 residues of a total of 344 have been solved [19]. The catalytic domain of PKCh possesses a high degree of homology ( 80%) with the catalytic domain of other isoforms of PKC belonging to ... recently solved at high resolution [19]. In this study we used IR spectroscopy to study the secondary structure of the catalytic domain of PKCf and the effect of its substrate, MgATP, and also to study the ... that the disappearance of the a-helix component is associated with the appearance of the intermolecu- larly aggregated b-sheet. When the correlation was made in the presence of MgATP (Fig. 5B), there...

Ngày tải lên: 16/03/2014, 13:20

... purposes) carcinostatic effects of ascorbic acid and its use as a thera- peutic agent to prevent the growth of cancer cells, there is still controversy over the effects of ascorbic acid. Accord- ing to the work done ... (Figure 3A). The average body weight at the 18 th day was 25.2 g of Group E, 24.8 g of Group F and 26 g of Group G respectively. These data showed that the treatments of ascorbic acid by challeng- ing ... administra- tion of high dose concentrations of ascorbic acid (1.7 × 10 -4 mol) to cancer patients in the terminal stage improved the quality of life and extended their lives [4]. Although there are...

Ngày tải lên: 18/06/2014, 15:20

... measures the relative slope variation of the eigenvalues {λ k } k=1, ,M .Thedifference be- tween the current eigenvalue and the mean of the next ones has been preferred to the simple subtraction of the ... re- lated to the resolution achieved, especially when the num- ber of available samples is reduced, and to the choice of the weighting windows, which controls the sidelobe le vel. Fur- thermore, there ... from their difference allows quite satisfactorily the estimation of N. 3. PARTICULAR CASE OF A LINEAR PIECEWISE VARIATION OF THE AUTOCORRELATION MATRIX EIGENVALUES The theoretical validity of this...

Ngày tải lên: 23/06/2014, 01:20

Ngày tải lên: 09/08/2014, 20:20

Ngày tải lên: 09/08/2014, 20:22

... During the therapeutic process, real-time estimation of the therapeutic effect was carried out by the computer system through the graphic changes in the target field and the hyperecho of the ... monitoring the energy deposition and the therapeutic effect, and also controlling the US exposure based on the feedback digital data from the ultrasonograms in the process of HIFU treatment. The therapeutic ... transducer. The system was operated using one of the several therapeutic transducers with the focal length of 90 to 160 mm. For each focal length, there is a choice of two transducers depending on the...

Ngày tải lên: 25/10/2012, 11:18

... role in the regulation of proliferation, migration, and differ- entiation of the epithelial cells [2,12,18,19] . Following the surgical treatment of adult periodontitis, the epithelial regeneration ... gingivitis grade of 0 or 1. Thanks to the bacteriostatic properties of the tested polymer, a more effective control of the surgical wound no bacterial contamina- tion of the surgical site ... largely responsible for the consistency of the active component that can act as a barrier to the spread of any bacteria penetrating the tissues including those of the periodontium. It is conceivable...

Ngày tải lên: 03/11/2012, 11:35

... CONCLUSION (1) The optimum operational condition of the device is a pressure of 0.3 MPa. When a high- dissolved CO 2 device is used for the disinfection of the effluent from the final settling ... point.) To be sure of the effect of SS in raw water, another experiment was conducted with the supernatant from the final settling tank of the WWTP (Ube City, Japan) with the same initial TC ... inhibited the uptake of CO 2 by the bacteria, leading to the slower FC inactivation. The results also revealed that it was possible to reach a high FC log-inactivation of 2.8 for the canal...

Ngày tải lên: 05/09/2013, 10:15