... undergo an important conformational change that brings loops A and B into close contact In the present work, and in order to obtain more information on the mechanism by which adenine restores the ... preincubated for 10 with MgCl2 before addition of adenine and application of pressure In the second, ADHR1 was preincubated with adenine for 10 before addition of MgCl2 and application of pressure ... 38, 381–393 Gilbert W (1986) The RNA world Nature 319, 618 Gesteland RF, Cech TR & Atkins JF (1999) In The RNA World Cold Spring Harbor Laboratory Press, New York, NY ´ Salehi-Ashtiani K, Luptak...
Ngày tải lên: 07/03/2014, 00:20
... stored at C All samples were centrifuged at 20 000 g during at 15 C prior to the SANS experiments High- pressure cell During the SANS experiments the protein solutions were contained in a high- pressure ... information is important in order to understand the properties of proteins under pressure In the future it would be benecial to perform SANS measurements at higher pressures in order to determine the ... 42 Pressure eects on azidometmyoglobin (Eur J Biochem 269) 4737 A high- pressure laser ash photolysis study J Biol Chem 264, 1889618901 Uchida, T., Ishimori, K & Morishima, I (2000) Unusual pressure...
Ngày tải lên: 08/03/2014, 10:20
Báo cáo Y học: Substrates modulate the rate-determining step for CO binding in cytochrome P450cam (CYP101) A high-pressure stopped-flow study pdf
... decrease or increase with increasing pressure As an example, Fig demonstrates the results for 1R-camphor, where the rate increases with pressure, and for norbornane, where the rate decreases with pressure ... cm3Æmol)1 for the CO binding in the 1R-camphor-bound P450cam In contrast, the activation volumes are positive for the norcamphor-bound as well as for the norbornane-bound proteins (% +8 cm3Æmol)1 for ... 1R-camphor (0.97); norcamphor (0.71) and norbornane (0.93) 100mM potassium phosphate buffer, pH 7.3, 10% (w/w) glycerol, substrate dissociation constants [3]: norcamphor (345 lM), norbornane (47 lM),...
Ngày tải lên: 08/03/2014, 23:20
Báo cáo Y học: Fluorescence study of the high pressure-induced denaturation of skeletal muscle actin pdf
... at 290 nm for a 1% solution of 6.6 for ATP-G-actin [20] High pressure apparatus High pressure devices used for this study consisted of a thermostated high pressure vessel equipped with sapphire ... of the pressure- induced denaturation of eATP G-actin (or eADP±F-actin), samples were kept at elevated pressures, and the changes in the ¯uorescence intensity under pressure were monitored The ... NaN3 The pressure was maintained for after reaching the indicated pressure as indicated by the arrows ¯uorescence evidently corresponded to the dissociation of eADP bound to F-actin For G-actin...
Ngày tải lên: 24/03/2014, 00:21
Báo cáo Y học: High pressure-induced changes of biological membrane Study on the membrane-bound Na+/K+-ATPase as a model system pdf
... these high pressures Hydrogen±tritium exchange under high pressure Effects of high pressure on irreversible tritium incorporation into Na+/K+-ATPase are shown in Fig Tritium incor- Fig Eect of pressure ... to 345 nm with increasing pressure, up to 220 MPa, and then decreased for pressures of 220 MPa or higher, being independent on ligands, Na+ and /or K+ However, the ¯uorescence intensity showed ... MPa or higher and FITC becomes hydrophilic with increasing pressure To examine reversibility of pressure- induced ¯uorescence changes, BIPM and FITC ¯uorescence intensities were measured after pressure...
Ngày tải lên: 24/03/2014, 00:21
Báo cáo khoa học: Charging of tRNA with non-natural amino acids at high pressure potx
... for the aminoacylation reaction of tRNA at high pressure remains unknown Recently, we obtained new information about the conformation of tRNA at elevated pressure [26] It is known that high pressure ... (Cl-Tyr), l-ornithine (l-Orn), d-ornithine (d-Orn), 3,4-dihydroxyphenylalanine (DOPA) and p-chloro-phenylalanine (Cl-Phe) was analysed using PAGE (Fig 1A,B) The amounts of amino acid incorporated ... Phe at high pressure without the need for a specific aa-tRNA synthetase Aminoacylation occurred only at the 3¢-end of tRNA [22] and pressure- aminoacylated Phe–tRNAPhe was a normal substrate for peptide...
Ngày tải lên: 30/03/2014, 10:20
báo cáo hóa học:" Modified femoral pressuriser generates a longer lasting high pressure during cement pressurisation" pot
... resected femoral neck With more controlled and reduced cement leakage the pressure drops more gradually allowing the high pressure to be retained for longer The time with high cementation pressure ... Discussion The modified pressuriser generates longer high pressure durations in the femoral model The pressure was homogenous within the femoral cavity for both pressurisers Increases in cement pressurisation ... penetration [6,7,11-16] while constant high pressure benefits high viscosity cement penetration whether in a constrained cavity such as the femur [17] or a more open area such as the acetabulum...
Ngày tải lên: 20/06/2014, 07:20
Báo cáo hóa học: " Landmine Detection and Discrimination Using High-Pressure Waterjets" potx
... from the high- pressure pump) or the waterjet exiting the nozzle For the purposes of the current discussion, we will assume that this component can either be neglected or has been filtered beforehand ... {classification} (15) LABORATORY DATA Mine detection algorithms were tested both using laboratory data and field data Laboratory data was used to test the algorithms’ ability to detect when an object was ... our laboratory tests shown earlier, preliminary studies in the laboratory indicate that the angle should not prevent detection and discrimination The more shallow firing angle was required for other...
Ngày tải lên: 23/06/2014, 01:20
Báo cáo khoa học: "Hydraulic conductance of root and shoot measured with the transient and dynamic modes of the high-pressure flowmete" ppsx
... [6], the root pressure probe [18], the potometer [29], the negative pressure flow system for root sections [14], the high pressure flowmeter (HPFM) [25, 27] Each method was developed for a particular ... each point corresponds to one record For the transient mode, pressure was increased at a rate of 5–8 kPa s–1 and kr was estimated from the slope of the last points For dynamic mode, pressure was ... on Quercus robur L Acorns were sowed in February in 5-liter pots filled with sandy soil (B-horizon) from the Mondon forest (North-East of France) and seedlings were grown for months in a greenhouse...
Ngày tải lên: 08/08/2014, 14:21
Báo cáo khoa học: "Whole shoot hydraulic resistance in Quercus species measured with a new high-pressure flowmeter" pps
... at 0.2 MPa pressure for or h Initially, flow rate was high but declined gradually The initially high flow rate was attributed to negative leaf water potentials, ψ However, after or h the leaf ... cut = Prior to connecting shoots to the highpressure flowmeter described below, all cut surfaces were shaved with a razor blade to remove blockage of cut vessels by cell-wall fragments formed by ... drought, may be important for a better understanding of adaptation to drought or However, differences in stem resistanccould account for differences in growth rate under mild drought Higher stem resistances...
Ngày tải lên: 08/08/2014, 23:22
Báo cáo khoa học: " Accelerated high-dose radiotherapy alone or combined with either concomitant or sequential chemotherapy; treatments of choice in patients with Non-Small Cell Lung Cancer" pps
... older and had more often an ECOG performance score more than At multivariate analysis only treatment with chemo-radiation was left as a prognostic factor for OS (P = 0.024) The HR for OS of sequential ... with chemoradiation Performance status and age are well known as prognostic factors for survival [21,35-37] After correction for treatment modality, the significance of age and performance status ... influence of repopulation [44] Several other studies report about improved results while shortening the OTT, for radiotherapy alone or for chemoradiation [18,45-47] Assuming that accelerated repopulation...
Ngày tải lên: 09/08/2014, 10:21
ASTM D129 Standard Test Method for Sulfur in Petroleum Products (General High Pressure Decomposition Device Method)
Ngày tải lên: 20/10/2014, 17:16
Báo cáo y học: " High blood pressure, antihypertensive medication and lung function in a general adult population"
... high blood pressure For example, subjects with currently normal blood pressure under medication might have had high blood pressure for a long time before it was recognized and treated Therefore, ... A HBP: high blood pressure regardless of its medical treatment - B HBP or medication: high blood pressure or the use of antihypertensive medication - C HBP and medication: high blood pressure ... 0.65 0.83 SBP: systolic blood pressure; DBP: diastolic blood pressure; HBP: high blood pressure (≥140/90 mmHg); FEV1: forced expiratory volume in one second; FVC: forced vital capacity *Medication:...
Ngày tải lên: 25/10/2012, 10:45
Báo cáo y học: " High-intensity non-invasive positive pressure ventilation for stable hypercapnic COPD
... used for high- intensity NPPV, preferably in a pressure- limited mode [7, 8, 9] The major target for the ventilatory adjustments (mainly increasing IPAP and respiratory rate) is to achieve normocapnia ... the patient is able to tolerate NPPV for more than two hours, further ventilator adjustments are performed in order to optimise alveolar ventilation according to the results of arterial blood ... Software, Inc., Point Richmond, California, USA) Mean values ± standard deviation were given after testing for normal distribution (Kolmogorov-Smirnov test) For non-normally distributed data, the median...
Ngày tải lên: 03/11/2012, 11:30
Cao huyết áp - High Blood Pressure
... Cao huyết áp (Tăng huyết áp )High Blood Pressure Cách điều trị cao huyết áp? Có số loại thuốc mà người bệnh dùng hàng ngày để kiểm...
Ngày tải lên: 18/03/2013, 09:45
Low- or Medium-pressure UV Lamp Inactivation of Microcystis aeruginosa
... is damaged, those organisms can not reproduce themselves and therefore the growth is inhibited However, when UV-damaged DNA is repaired by photoreactivation process, the microorganisms can reproduce ... extraction was performed according to the protocols provided by Qiagen, with a minor modification The extracted DNA was concentrated by using centrifugal filter devices (Centricon; Millipore) and resuspended ... Trihalomethane precursors J Am Water Works Assoc 72: 344-350 10) Oppenheimer, J A., J G Jacangelo, J -M Laine, and J E Hoagland (1997) Testing the equivalency of ultraviolet light and chlorine for disinfection...
Ngày tải lên: 05/09/2013, 09:08
High-intensity Low-pressure Electrodeless Mercury-Argon Lamp for UV Disinfection of Wastewater
... of the coldest spot of the lamp determines the Hg vapor pressure One can therefore use that temperature as a parameter representing Hg vapor density in the discharge The dependence of the 254nm ... installation of the sewage effluent disaffection system for wastewater treatment is shown in Fig The disinfection system is a standard product incorporating a set of electrodeless UV lamps By combining ... for protective quartz sleeves (See fig 3) Structurally, each current path is composed of UV lamps, a submerged weir and an overflow weir to pass the sewage around the lamps uniformly Therefore,...
Ngày tải lên: 05/09/2013, 09:08
Tài liệu High Availability Campus Network Design—Routed Access Layer using EIGRP or OSPF doc
... Cisco Express Forwarding (CEF) forwarding adjacency entries Before a failure, traffic is being forwarded using both of these forwarding entries On failure of an adjacent link or neighbor, the switch ... EIGRP neighbor information for each access switch and look for the flag indicating queries being suppressed Distribution#sh ip eigrp neighbors detail gig 3/3 IP-EIGRP neighbors for process 100 ... routing or RIP, or a design that leverages a combination of routing protocols (for example, RIP redistributed into OSPF) High Availability Campus Network Design— Routed Access Layer using EIGRP or...
Ngày tải lên: 24/01/2014, 10:20
Tài liệu Báo cáo khoa học: Reversible tetramerization of human TK1 to the high catalytic efficient form is induced by pyrophosphate, in addition to tripolyphosphates, or high enzyme concentration ppt
... tetrameric and dimeric forms in Figs and exhibited low or high catalytic efficiency Therefore, the various incubated hTK1 forms from Figs and were analyzed for their kinetic behavior with dThd, and ... dithiothreitol), incubated with or without mm of the respective nucleotide or phosphate compound for h at °C, and stored for at least weeks at )80 °C before use for kinetic and molecular mass analyses ... more and more enzyme will be in the high- active tetramer form Recently, phosphorylation of hTK1 at serine 13 has been proposed to be involved in this regulation, preventing ATP-induced transformation...
Ngày tải lên: 18/02/2014, 12:20