hepatitis b virus replication mutation and evolution

... (1987) The molecular biology of the hepatitis < /b> B viruses Annu Rev Biochem 56, 651–693 Antonucci TK & Rutter WJ (1989) Hepatitis < /b> B virus (HBV) promoters are regulated by the HBV FEBS Journal 277 (2010) ... that the FXRa and JNK ⁄ c-Jun pathways may be elevated Bile acid metabolism and HBV gene expression and not only might HBV gene expression consequently be increased, but also the anti-HBV effects ... cells and the HBx and HBV core mRNA levels and then the FXRa mRNA levels were determined via RT-PCR The RT-PCR bands were quantified and normalized relative to the b- actin mRNA control band using...

Ngày tải lên: 22/03/2014, 21:21

... apoptotic effect by HBV genome A, B, and C Flow cytometry analysis of apoptotic effect by HBV genome A, B, and C HepG2 cells were transiently transfected with HBV genome A, B, and C and incubated for ... References HBSP A BH3 HBSP B BH3 HBSP C BH3 21 31 32 35 LEEELPRLADADLHR LEEELPRLADEDLNH LEEELPRLADEGLNR Figure Alignment of BH3 domains of HBSPs Alignment of BH3 domains of HBSPs Alignment of the BH3 ... characterization of hepatitis < /b> B virus surface antigen mutants in Singapore patients with hepatocellular carcinoma and hepatitis < /b> B virus carriers negative for HBsAg but positive for anti-HBs and anti-HBc J Gastroenterol...

Ngày tải lên: 18/06/2014, 18:20

... apoptotic effect by HBV genome A, B, and C Flow cytometry analysis of apoptotic effect by HBV genome A, B, and C HepG2 cells were transiently transfected with HBV genome A, B, and C and incubated for ... References HBSP A BH3 HBSP B BH3 HBSP C BH3 21 31 32 35 LEEELPRLADADLHR LEEELPRLADEDLNH LEEELPRLADEGLNR Figure Alignment of BH3 domains of HBSPs Alignment of BH3 domains of HBSPs Alignment of the BH3 ... characterization of hepatitis < /b> B virus surface antigen mutants in Singapore patients with hepatocellular carcinoma and hepatitis < /b> B virus carriers negative for HBsAg but positive for anti-HBs and anti-HBc J Gastroenterol...

Ngày tải lên: 20/06/2014, 01:20

... 132bp 109bp Counts of samples B B C D Genotype of HBV Figure of HBV reactive blood donors Genotyping Genotyping of HBV reactive blood donors All positive samples from the blood donors were subjected ... consent was obtained from the participants HBV serological marker determination Testing for HBV serological markers, including HBs, antiHBs, anti-HBe, HBe and anti-HBs, were performed by ELISA using ... positive (Table 2) Although HBsAg in donor was nega- tive (Table 2), HBV DNA testing proved there were few virus copies (Fig 1B) The major HBV strains in local donors were B and C genotypes HBV strains...

Ngày tải lên: 12/08/2014, 04:20

... et al Hepatitis < /b> C virus antibody and hepatitis < /b> C virus replication in chronic hepatitis < /b> B patients J Hepatol 1994;21:509-514 Liaw YF Role of hepatitis < /b> C virus in dual and triple hepatitis < /b> virus ... 1983;11:191-205 Bradley DW, Maynard JE, McCaustland KA, Murphy BL, Cook EH, Ebert JW Non-A, non -B hepatitis < /b> in chimpanzees: interference with acute hepatitis < /b> A virus and chronic hepatitis < /b> B virus infections ... Galimany R, Esteban R, et al Role of hepatitis < /b> B, C, and D viruses in dual and triple infection: influence of viral genotypes and hepatitis < /b> B precore and basal core promoter mutations on viral...

Ngày tải lên: 02/11/2012, 09:51

... chronic hepatitis < /b> C to interferon therapy and disease progress HBeAg-negative CHB HBeAg-negative chronic hepatitis < /b> B (e-CHB), characterized by HBV DNA levels detectable by nonamplified assays and ... biological differences between hepatitis < /b> B viruses of genotype B and C Hepatology 2001;33:218-223 Chu CJ, Hussain M, Lok AS Hepatitis < /b> B virus genotype B is associated with earlier HBeAg seroconversion ... Asian hepatitis < /b> B virus carriers J Infect Dis 1999; 179:775-782 Ding X, Mizokami M, Yao G, Xu B, Orito E, Ueda R, et al Hepatitis < /b> B virus genotype distribution among chronic hepatitis < /b> B virus...

Ngày tải lên: 02/11/2012, 11:12

... infection establishes, the serology markers like HBeAg, HBeAb and HBsAb can be positive or negative except HBsAg and HBcAb (IgG form) remain positive HBeAg positive chronic hepatitis < /b> B Age at the ... liver begins to drop With clearance of the infection, HBsAg and HBeAg disappear, and free HBsAb become detectable Presence of HBsAg, HBeAg, and high DNA level for greater than months implies progression ... which seroconversion of HBeAg to HBeAb occurs, HBV DNA becomes non-detectable or at low level and ALT is usually normal, reflecting very low or no replication of HBV and mild or no hepatic injury...

Ngày tải lên: 02/11/2012, 11:17

... A, Pol S, Brechot C & Kremsdorf D (2003) The expression of hepatitis < /b> B spliced protein (HBSP) encoded by a spliced hepatitis < /b> B virus RNA is associated with viral replication and liver fibrosis J ... HP1 and HP2 regions are indicated, with stems being marked by cyan bars and the RNase-accessible joint and loop regions by red bars (D) RNase footprinting analysis of products from the ribonuclease ... Western blot analysis of the PTB and nPTB protein level in HepG2-wh cells, with b- actin as a loading control (B) PTB RNAi and overexpression in HEK293T cells RNAi and overexpression of PTB were...

Ngày tải lên: 28/03/2014, 23:20

... Serological markers Serologic markers for HBV (hepatitis < /b> B surface antigen [HBsAg], hepatitis < /b> Be antigen [HBeAg] and antibodies to hepatitis < /b> B core antigen [anti-HBc]) (enzyme immunoassay [EIA]; Adaltis, ... number not for citation purposes) Virology Journal 2007, 4:74 http://www.virologyj.com/content/4/1/74 Table 1: Primer First PCR P1 S1-2 Second PCR Mix A B2 BA1R BB1R BC1R Mix B BD1 BE1 BF1 B2 R ... primers BA1R (type A specific), BB1R (type B specific), and BC1R (type C specific) B2 R was used as the inner primer (antisense) with a combination called mix B for genotypes D, E, and F Mix B consisted...

Ngày tải lên: 18/06/2014, 18:20

... Perre P, Cartoux M, Mandelbrot L, Dabis F: HBs antigen carrier state in pregnant women in Bobo Dioulasso (Burkina Faso)] Dakar Med 2000, 45:188-190 Simpore J, Ilboudo D, Samandoulougou A, Guardo ... SF: Parallel and overlapping HIV and bloodborne hepatitis < /b> epidemics in Africa Int J STD AIDS 2004, 15:145-152 McCarthy MC, el-Tigani A, Khalid IO, Hyams KC: Hepatitis < /b> B and C in Juba, southern ... correlation between these two infections, which could influence the evolution of these diseases [11,12] Parallel and overlapping HIV and blood borne hepatitis < /b> epidemics in Africa and Influence...

Ngày tải lên: 18/06/2014, 18:20

... Serological markers Serologic markers for HBV (hepatitis < /b> B surface antigen [HBsAg], hepatitis < /b> Be antigen [HBeAg] and antibodies to hepatitis < /b> B core antigen [anti-HBc]) (enzyme immunoassay [EIA]; Adaltis, ... number not for citation purposes) Virology Journal 2007, 4:74 http://www.virologyj.com/content/4/1/74 Table 1: Primer First PCR P1 S1-2 Second PCR Mix A B2 BA1R BB1R BC1R Mix B BD1 BE1 BF1 B2 R ... primers BA1R (type A specific), BB1R (type B specific), and BC1R (type C specific) B2 R was used as the inner primer (antisense) with a combination called mix B for genotypes D, E, and F Mix B consisted...

Ngày tải lên: 20/06/2014, 01:20

... Perre P, Cartoux M, Mandelbrot L, Dabis F: HBs antigen carrier state in pregnant women in Bobo Dioulasso (Burkina Faso)] Dakar Med 2000, 45:188-190 Simpore J, Ilboudo D, Samandoulougou A, Guardo ... SF: Parallel and overlapping HIV and bloodborne hepatitis < /b> epidemics in Africa Int J STD AIDS 2004, 15:145-152 McCarthy MC, el-Tigani A, Khalid IO, Hyams KC: Hepatitis < /b> B and C in Juba, southern ... correlation between these two infections, which could influence the evolution of these diseases [11,12] Parallel and overlapping HIV and blood borne hepatitis < /b> epidemics in Africa and Influence...

Ngày tải lên: 20/06/2014, 01:20

... [9,10], Hepatitis < /b> Virus Database [11], SEQHEPB [12], and the HepSeq database [13] Each has its own focus and utility The viral RefSeq genome project is broad but includes 10 Hepadnaviridae members ... MySQL database HBVRegDB To identify conserved viral genomic regions, three blast queries (blastn, tblastx and blastx) were performed on RefSeq Virus release 24 with the parameters shown in Table The ... representative HBV sequences from these groups of alignments is available using Gbrowse For HBVRegDB the GBrowse software package was chosen because of its flexible configuration and efficient handling...

Ngày tải lên: 20/06/2014, 01:20

... Organization and Policy, School of Public Health, University of Alabama at Birmingham, Birmingham, Alabama, USA Department of Epidemiology, School of Public Health, University of Alabama at Birmingham, Birmingham, ... in their diets and examined the association of vitamins A and E concentrations, HIV status, AF-ALB levels and hepatitis < /b> B virus (HBV) infection Methods Study location, design and target population ... standard deviation (SD) and median concentrations of micronutrients, AF-ALB and liver function tests for HIV-negative and HIV-positive participants are shown in Table Vitamins A and E and AFALB...

Ngày tải lên: 20/06/2014, 08:20

... measured at 615 nm) (B) The emission spectra of Tb3+ chelate (green line) and Eu3+ nanoparticles (orange line) HIV-1 env) and chemically biotinylated mAb 2 1B (BiomAb 2 1B) , immobilized on SA-coated ... (anti-HIV-1 antibody and HBsAg) in the absence (single-label) and presence (dual-label) of the binders of the other analyte Using rHBsAg (subtype adw), ranging from 0.02-200 ng/mL, the dual-label assay ... single and dual-label assays are represented by the filled star and empty square symbols, respectively (B) AntiHIV-1 antibody detection Tb3+ fluorescence data for the single label and dual-label...

Ngày tải lên: 11/08/2014, 00:22

... understanding the evolutionary trends of HBV genotypes Keywords: Hepatitis < /b> B virus, codon usage pattern, evolution, mutation pressure, translation selection Introduction Hepatitis < /b> B virus (HBV) ... might be one of factors to drive HBV evolution The effect of mutation pressure on codon usage of HBV To analyze if the evolution of HBV is shaped by mutation pressure from virus itself or by translation ... pattern and evolutional dynamics of HBV as well as the relationship between codon usage pattern of HBV and its host Result Synonymous coodn usage in HBV The C% and U% were higher than A% and G%, and...

Ngày tải lên: 11/08/2014, 21:22

... Nussbaum O, Galun E: Small interfering RNA inhibits hepatitis < /b> B virus replication in mice Mol Ther 2003, 8:769-776 Ying C, De Clercq E, Neyts J: Selective inhibition of hepatitis < /b> B virus replication ... interference is mediated by 2 1and 22-nucleotide RNAs Genes Dev 2001, 15:188-200 Li GQ, Gu HX, Li D, Xu WZ: Inhibition of Hepatitis < /b> B virus cccDNA replication by siRNA Biochem Biophys Res Commun 2007, ... and electrophoretically transferred onto polyvinylidene difluoride (PVDF) membrane (Hybond-P, Amersham Pharmacia Biotech) Blots were blocked with 5% of skim milk in TBS-T buffer for h and subsequently...

Ngày tải lên: 12/08/2014, 01:21

... USA) and the primers described previously [4] pCMV-HBc (WT), pCMV-HBc (L60V), pCMV-HBc (S87G) and pCMV-HBc (I97L) were confirmed by sequencing and these vectors were able to express the HBc protein/Flag-tag ... whether the inhibition of MxA transcription by HBc protein would decrease the inhibitory effect of IFN-a on HBV replication pCMV-HBc (WT), pCMV-HBc (L60V), pCMVHBc (S87G), pCMV-HBc (I97L) and pCMV-Tag1 ... added and incubation continued for a further 24 h at 37°C Then, the relative MxA and GAPDH mRNA levels, HBV DNA and hepatitis < /b> B surface antigen (HBsAg) were assessed as described below Western blot...

Ngày tải lên: 12/08/2014, 01:22

... changes by single mutation in the hepatitis < /b> B virus surface antigen (HBsAg) identified in HBsAg-negative blood donors Virology Journal 2010 7:326 Submit your next manuscript to BioMed Central and ... InterPro Bioinformatics 2001, 17:847-848 16 Mulder NJ, Apweiler R, Attwood TK, Bairoch A, Barrell D, Bateman A, Binns D, Biswas M, Bradley P, Bork P, Bucher P, Copley RR, Courcelle E, Das U, Durbin ... mothers with HBV infection, and liver transplant recipients treated with hepatitis < /b> B immunoglobulin (HBIg) therapy [3-5] Recognition of the ’a’ determinant by antibody against HBsAg (anti-HBs) depends...

Ngày tải lên: 12/08/2014, 02:20

... mutant hepatitis < /b> B virus in carriers Hepatol Res 2002, 23:48-54 Yoo B, Park J, Kim HJ, Lee DH, Chan YJ, Park SM: Precore and core Promotor Mutations of Hepatitis < /b> B Virus and Hepatitis < /b> B e AntigenNegative ... M: Hepatitis < /b> B virus genotype distribution among chronic hepatitis < /b> B virus carriers in Shanghai, China Intervirology 2001, 44:43-47 Mayerat C, Mantegani A, Frei PC: Does hepatitis < /b> B virus (HBV) ... Sarrami-Forooshani R, Sabahi F, Sabouri E, Tavangar HR, Azizi M, Mahboudi F: Hepatitis < /b> B virus genotyping, core promoter, and precore/core mutations among Afghan patients infected with hepatitis < /b> B: a preliminary...

Ngày tải lên: 12/08/2014, 04:20