hepatic transplantation of mesenchymal stem cells in rodent animal models

... reviewed in detail in the following part regarding the animal models of PD INTRODUCTION Animal models Animal models are an important aid to study pathogenic mechanisms and therapeutic strategies in ... stain positively for ubiquitin and α-synuclein.Ubiquitin is a protein involved in the degradation of cytoplasmic proteins The function of α-synuclein is still not clear In the healthy brain, ... unclear In this chapter, the current understanding of etiology and pathogenesis of INTRODUCTION PD, the application of animal models for studying PD, and possible therapeutic application of stem cells...

Ngày tải lên: 11/09/2015, 09:05

... were included in the study Animals were inbred in the experimental animal unit, Faculty of Medicine, Cairo University Rats were maintained according to the standard guidelines of Institutional Animal ... of MSCs to cancer cells, nature of tumour cells and cancer stem cells, integrity of immune system, number of stem cell passages and site of injection; all can affect the outcome of MSCs use in ... tissues by assessing the gene expression profile of some of the Wnt signaling target genes:cyclin D, PCNA, survivin, b-catenin Methods Ninety albino female rats inbred strain (Cux1: HEL1) of matched...

Ngày tải lên: 10/08/2014, 10:21

... inhibition of IκB kinase Page of 15 [13,18] There is increasing interest in curcumin as a therapeutic option for OA and RA, with evidence that curcumin inhibits the IL-1β-induced activation of ... the action of pro-inflammatory cytokines in the joint thus disrupting the inflammatory cycle inhibit IL-1β induced activation of degradative pathways in MSCs It must be noted that in this study ... positive chondrogenic signaling However, interaction of curcumin and TGF-β signaling in chondrocytes is an interesting point and will require further detailed investigations In the present study we...

Ngày tải lên: 12/08/2014, 14:22

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Ngày tải lên: 07/08/2014, 23:22

... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... noitcudni cinegoetso na desu ew ,sCSM eht morf noitaitnereffid cinegoetso ecudni oT laitnetop cinegoetso tibihxe sCSM doolb droC )A2 giF( nitnemiv dna 2HS snegitna detaler -myhcnesem rof ytivitisop...

Ngày tải lên: 07/08/2014, 18:21

... 3,3-diaminobenzidine disclosure) for aggrecan and the staining intensity increased with time post injection (Figures and 5b) Aggrecan matrix staining within the vicinity of the injected cells was ... following injection, and the intensity of the matrix staining increased with time in culture (Figures and 5b) Weak staining for type II collagen was observed within a small number of cells in monolayer ... fluorescent protein (GFP)-infected cells Following identification of the position of injection site and the presence of GFP-labelled cells, serial sections in the area of the injection site were mounted...

Ngày tải lên: 09/08/2014, 01:22

... self-antigen-reactive T cells in vivo [38] The upregulation of IL-10 is in line with the induction of Tregs in vitro and in vivo in our study Moreover, in our experiments we did not find UC-MSCs in the joint of the ... differentiation of UC-MSCs staining for alkaline phosphatase Arrows indicate the accumulation of intracytoplasmic alkaline phosphatase of osteoblast Original magnification × 40 involved in the suppression of ... that administration of UC-MSCs attenuated systemic inflammation in CIA in mice UC-MSCs downregulated the production of the proinflammatory cytokines TNF-a, and IL-6 in vitro and in vivo In addition,...

Ngày tải lên: 12/08/2014, 15:22

... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... differentiation of human mesenchymal stem cells on laminin-5 J Cell Biochem (2):499-514 102 Lozito TP, Kuo CK, Taboas JM, et al 2009;Human mesenchymal stem cells express vascular cell phenotypes upon interaction ... 1991;Expression of modified bFGF cDNAs in mammalian cells Ann N Y Acad Sci :149-160 120 Kolf CM, Cho E, Tuan RS 2007 ;Mesenchymal stromal cells Biology of adult mesenchymal stem cells: regulation of niche,...

Ngày tải lên: 11/09/2015, 09:16

... using the Adobe Photoshop 7.0 software Statistics on bioluminescent signal intensity was obtained using WinView software according to the software instruction For comparison of tumor targeting ... erbB2 expressing cells MSC-AR bind to erbB2-expressing cells in vivo Differential kinetics of MSC lung clearing in a transient transgenic model We next investigated if membrane expression of the AR ... erbB2-expressing lung cells in vivo in transient transgenic model (A) Transient expression of erbB2 in the lungs of hCAR mice was induced by iv injection of AdCMVerbB2 Expression of erbB2 exclusively in...

Ngày tải lên: 20/06/2014, 07:20

... months of age demonstrated engraftment of Y chromosome positive cells (7.4% of total cells) [52] These findings prove that allogeneic mesenchymal cells can engraft and differentiate into bone Clinical ... Pozzi S, et al.: Interaction of human mesenchymal stem cells with cells involved in alloantigen-specific immune response favors the differentiation of CD4+ T-cell subsets expressing a regulatory/suppressive ... B, Sundin M, Rosendahl K, Tammik C, Ringden O: Mesenchymal stem cells inhibit the expression of CD25 (interleukin-2 receptor) and CD38 on phytohaemagglutinin-activated lymphocytes Scand J Immunol...

Ngày tải lên: 09/08/2014, 08:23

... members of A Disintegrin And Metalloprotease family ADAM proteins contain a disintegrin and MMP domain, which has the dual function of cleavage/release of cell-surface proteins and remodelling of ... reporting expression of integrins α1, α2, α3, α5, α6, α10, αv, β1, β3 and β5 [52] Integrin α5β1 is the most prominently expressed integrin in chondrocytes and integrin α10β1 is the dominant collagenbinding ... However, no information is available on the role of integrins α4, α7, β5 and β7 during chondrogenesis Integrins containing the α4 or α7 subunit bind to RGD-containing components, namely fibronectin and...

Ngày tải lên: 09/08/2014, 10:20

... adenoviral infection or osmotic pumps in inhibiting bleomycin-induced increases in TNF-α, IL-1α, and trafficking of lymphocytes and neutrophils into the lung [56] These successful animal studies ... levels of the donor cells at the same time points [75] In studies with different models of induced arthritis, including a traumainduced goat OA model [73] and a mouse model of collageninduced ... the cytokine secretion profile of dendritic cells, naïve and effector T cells, and NK cells to induce a more anti-inflammatory or tolerant phenotype Secretion of the proinflammatory cytokines, TNF-α...

Ngày tải lên: 09/08/2014, 13:22

... Regulation of stemness and stem cell niche of mesenchymal stem cells: implications in tumorigenesis and metastasis J Cell Physiol 2010, 222:268-277 Ohishi M, Schipani E: Bone marrow mesenchymal stem cells ... droplets was observed in these cells [12] In view of these features, we further investigated the possibility of HPB-AML-I being a neoplasm of MSC origin Recently, some human MSC lines have been established ... MSCs Inverted microscopic examination (Figure 1A) and May Grünwald-Giemsa staining (Figure 1B) of HPBAML-I cells revealed that this cell line is composed of round-polygonal and spindle-like cells...

Ngày tải lên: 10/08/2014, 10:20

... role of stem cells in OSA, although there have been recent reports of the detection of circulating endothelial progenitor cells in patients [18-20] and circulating MSC in a rat model [8] Accordingly, ... pro-inflammatory cytokines in apneic serum Effectively, IL1-β and TNF-α, which increase in the serum of animals in OSA models and in that of patients with this sleep disorder, have proved to increase the ... J, Lin GS, Bao CY, Hu ZM, Hu MY: Anti-inflammation role for mesenchymal stem cells transplantation in myocardial infarction Inflammation 2007, 30:97-104 13 Tyndall A, Pistoia V: Mesenchymal stem...

Ngày tải lên: 12/08/2014, 11:22

... Despite our incomplete understanding of the mechanisms underlying these divergent results, and inspired by positive reports on the use of bone marrow-derived MSCs in the outcome of GVHD and transplantation ... disease; MSC, mesenchymal stem cell Page of Competing interests The authors have no competing interests Author details Department of Rheumatology, Leiden University Medical Center, Albinusdreef 2, ... apparent difficulties in recapitulating the in vitro effects in vivo, however, researchers should be cautioned and should remain critical concerning the use of MSCs for the treatment of human autoimmune...

Ngày tải lên: 12/08/2014, 14:21

... signal-enhancing system based on biotin in terms of a clearly lower background All antibodies were diluted in PBS containing 5% bovine serum albumin The signal was developed by incubation with ... of the osteogenic lineage, as assessed by the detection of alkaline phosphatase, was more pronounced in CD166+ cells, indicating more progenitor cells in CD166+ than CD166- cells (Figure 5c,d) ... sieve and washed twice in DMEM/F12 containing 5% FCS and antibiotics After counting in a Neubauer chamber, cells were seeded in culture flasks at an average density of × 104 cells/ cm2, passaged...

Ngày tải lên: 12/08/2014, 15:23

... Figure Mesenchymal stem cells used during this study Mesenchymal stem cells used during this study Typical morphological aspects of mesenchymal stem cells observed through culture flask (A) Mesenchymal ... perspective of cellular therapy by intravenous stem cells infusion The participation of stem cells in several vascular diseases pathogenesis was first proved with haematopoietic stem cells (HSCs) In this ... Spleen Infusion site Kidneys Bone 0% Early dynamic distribution of mesenchymal stem cells in vivo Figure Early dynamic distribution of mesenchymal stem cells in vivo Sequential whole body scintigraphies...

Ngày tải lên: 12/08/2014, 18:20

... The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics ... Foundation This type of federal research funding strategy involving existing embryonic stem cell lines is consistent with the President's belief in the fundamental value and sanctity of human life The ... destruction of additional human embryos Indeed, the embryos from which the existing ESCs originated have already been destroyed Federal funding of medical research on these existing stem cell lines...

Ngày tải lên: 13/08/2014, 23:20

... scattering F1 contained 4% of total protein input but contained ∼ 50% of the CD9 in the input (Fig 5B) Proteins were distributed among F2, F3, and F4 fractions according to the principle of size-exclusion ... reperfusion injury7 Based 13 on studies in animal models of AMI, reperfusion injury contributed up to 50% of the final infarct size7 Amelioration of reperfusion injury and subsequent reduction of myocardial ... numerous studies on the transplantation of MSCs in patients and animal models, insight into the mechanistic issues underlying the effect of MSC transplantation remains vague An often-cited hypothesis...

Ngày tải lên: 10/09/2015, 08:42

... administration of mesenchymal stem cells (MSC) has been shown to be efficacious in ameliorating disease conditions in animal models and clinical trials However the mechanism underlying MSC homing to injury ... Antigen (α4β1 integrin) ix Introduction 1.1 Mesenchymal stem cells Mesenchymal stem cells (MSC), otherwise known as bone marrow stromal cells, was discovered by Friedenstein who noticed that transplantation ... Mode of administration There are a few ways of introducing ex vivo expanded MSC into subjects Different routes of administration have varying degrees of invasiveness and specificity The three main...

Ngày tải lên: 13/10/2015, 15:54