hepatic differentiation of mesenchymal stem cells in vitro strategies

... inhibition of IκB kinase Page of 15 [13,18] There is increasing interest in curcumin as a therapeutic option for OA and RA, with evidence that curcumin inhibits the IL-1β-induced activation of ... mechanism of action of curcumin in MSCs and chondrocytes might involve the β1-integrin receptor signalling pathway Several studies have suggested that curcumin interacts with the TGF-β signaling cascade, ... TGF-β signaling pathway does not have an inhibitory effect on positive chondrogenic signaling However, interaction of curcumin and TGF-β signaling in chondrocytes is an interesting point and will...

Ngày tải lên: 12/08/2014, 14:22

... llec gnimrofrep dna esu lacinilc rof SBF gnisu tuohtiw oviv xe sCSM dnapxe ot sdohtem dnif ot deen ew dna ,hcraeser ruo rof SBF desu ew ,yduts tneserp ni ,revewoH noitarefilorp sCSM rof noitidnoc ... 001-X notirT %4.0 neht dna ,nim rof enoteca %001 ,nim 01 rof lonahtem %001 dloc eci htiw detaert neht erew yehT erutarepmet moor ta nim 01 rof SBP ni edyhedlamrof %7.3 htiw dexif neht dna SBP ... noitcudni cinegoetso na desu ew ,sCSM eht morf noitaitnereffid cinegoetso ecudni oT laitnetop cinegoetso tibihxe sCSM doolb droC )A2 giF( nitnemiv dna 2HS snegitna detaler -myhcnesem rof ytivitisop...

Ngày tải lên: 07/08/2014, 18:21

... 2001;Function and interactions of integrins Cell Tissue Res (3):285-298 41 Mould AP, Askari JA, Aota SI, et al 1997;Defining the topology of integrin alpha5beta1-fibronectin interactions using inhibitory ... 2007 ;In vitro enhancement of collagen matrix formation and crosslinking for applications in tissue engineering: a preliminary study Tissue Eng (2):385391 37 Qian L, Saltzman WM 2004;Improving ... for the osteogenic differentiation of human mesenchymal stem cells on laminin-5 J Cell Biochem (2):499-514 102 Lozito TP, Kuo CK, Taboas JM, et al 2009;Human mesenchymal stem cells express vascular...

Ngày tải lên: 11/09/2015, 09:16

... information is available on the role of integrins α4, α7, β5 and β7 during chondrogenesis Integrins containing the α4 or α7 subunit bind to RGD-containing components, namely fibronectin and vitronectin ... reporting expression of integrins α1, α2, α3, α5, α6, α10, αv, β1, β3 and β5 [52] Integrin α5β1 is the most prominently expressed integrin in chondrocytes and integrin α10β1 is the dominant collagenbinding ... corresponding mRNAs were increased on day 21, except for filamin (Figure 1a,b) Gelsolin is involved in the cytoskeletal organization and induced by integrin signalling The expression of gelsolin and...

Ngày tải lên: 09/08/2014, 10:20

... of MSCs to cancer cells, nature of tumour cells and cancer stem cells, integrity of immune system, number of stem cell passages and site of injection; all can affect the outcome of MSCs use in ... tissues by assessing the gene expression profile of some of the Wnt signaling target genes:cyclin D, PCNA, survivin, b-catenin Methods Ninety albino female rats inbred strain (Cux1: HEL1) of matched ... regulation of the transcription factor b-catenin, a key component of the Wnt signaling pathway [58] In the normal state, the binding of members of a family of soluble cysteine-rich glycoprotein ligands,...

Ngày tải lên: 10/08/2014, 10:21

... 3,3-diaminobenzidine disclosure) for aggrecan and the staining intensity increased with time post injection (Figures and 5b) Aggrecan matrix staining within the vicinity of the injected cells was ... following injection, and the intensity of the matrix staining increased with time in culture (Figures and 5b) Weak staining for type II collagen was observed within a small number of cells in monolayer ... co-culture of MSCs with NP cells in vitro is capable of inducing differentiation to an NP-like phenotype [26] This raises the possibility that the native IVD cells in vivo could induce MSC differentiation...

Ngày tải lên: 09/08/2014, 01:22

... ytisrevinU lanoitaN luoeS fo ’slaminA yrotarobaL fo esU dna eraC eht rof ediuG‘ eht fo senilediug eht ot gnidrocca demrofrep saw noitagitsevni sihT yduts yrujni droc lanips latnemirepxe eht rof desu ... erew sgod ehT ICS eht retfa keew a demrofrep saw sCSM-BCU eninac fo noitatnalpsnarT :FSCGhmr fo noitcejni dna sCSM-BCU eninac fo noitatnalpsnarT noitcejni eht rof noitulos enilas fo lµ 051 ni 601 ... ilumits cinegotim cificepsnon ro ralullec yb decudni noitarefilorp etycohpmyl-T sserppus sllec lamorts worram enob namuH MA innaiG ,S itnasirG ,P iccuettaM ,DP inognoL ,M isenaliM ,M ingaM ,C...

Ngày tải lên: 07/08/2014, 23:22

... self-antigen-reactive T cells in vivo [38] The upregulation of IL-10 is in line with the induction of Tregs in vitro and in vivo in our study Moreover, in our experiments we did not find UC-MSCs in the joint of the ... that administration of UC-MSCs attenuated systemic inflammation in CIA in mice UC-MSCs downregulated the production of the proinflammatory cytokines TNF-a, and IL-6 in vitro and in vivo In addition, ... Osteogenic differentiation of UC-MSCs staining for alkaline phosphatase Arrows indicate the accumulation of intracytoplasmic alkaline phosphatase of osteoblast Original magnification × 40 involved in...

Ngày tải lên: 12/08/2014, 15:22

... The end of the beginning for pluripotent stem cells Nature 2001, 414:92-97 23 24 25 26 Doerflinger RM: The ethics of funding embryonic stem cell research: a Catholic viewpoint Kennedy Inst Ethics ... Ratio of inner cell mass and trophoblastic cells in blastocysts derived from porcine 4- and 8cell embryos and isolated blastomeres cultured in vitro in the presence or absence of protein and ... destruction of additional human embryos Indeed, the embryos from which the existing ESCs originated have already been destroyed Federal funding of medical research on these existing stem cell lines...

Ngày tải lên: 13/08/2014, 23:20

... methods for inducing MSCs into neuron-like cells have been reported in vitro and the systemic injection of MSCs in mice with brain injury had positive effects on injury repair, incompatible genetic ... microglial cells in the SNc of MPTP-treated mice, real-time RT-PCR did not find mRNA expression of MBL in the brain parenchyma, suggesting the infiltration of MBL from the circulation into the brain ... administration results in specific degeneration of about 50%-93% of the dopaminergic neurons in the SNc and more than 99% loss of dopamine in the striatum The schematic drawing of mechanism(s) of...

Ngày tải lên: 11/09/2015, 09:05

... YAMAZAKI, AND SHIN-ICHI HAYASHI 98 v vi TABLE OF CONTENTS Development of Hematopoietic Repopulating Cells from Embryonic Stem Cells The In Vitro Differentiation of Mouse Embryonic Stem Cells into Neutrophils ... Analysis of Megakaryocytes from Murine Embryonic Stem Cells 11 Development of Lymphoid Lineages Embryonic Stem Cells In Vitro from 12 Probing Dendritic Cell Function by Guiding the Differentiation of ... an impure mixture of matrix proteins derived from porcine or bovine skin, on the growth of pluripotent cells are often overlooked Gelatin can increase the differentiation of EPL cells when compared...

Ngày tải lên: 11/04/2014, 00:32

... using the Adobe Photoshop 7.0 software Statistics on bioluminescent signal intensity was obtained using WinView software according to the software instruction For comparison of tumor targeting ... marker of tumor cells (MSC-AR) We have shown that these AR-expressing MSCs (MSC-AR) have enhanced binding to erbB2-expressing cells in vitro Furthermore, we tested erbB-2 targeting of MSC-AR in model ... erbB2 expressing cells MSC-AR bind to erbB2-expressing cells in vivo Differential kinetics of MSC lung clearing in a transient transgenic model We next investigated if membrane expression of the AR...

Ngày tải lên: 20/06/2014, 07:20

... months of age demonstrated engraftment of Y chromosome positive cells (7.4% of total cells) [52] These findings prove that allogeneic mesenchymal cells can engraft and differentiate into bone Clinical ... Pozzi S, et al.: Interaction of human mesenchymal stem cells with cells involved in alloantigen-specific immune response favors the differentiation of CD4+ T-cell subsets expressing a regulatory/suppressive ... B, Sundin M, Rosendahl K, Tammik C, Ringden O: Mesenchymal stem cells inhibit the expression of CD25 (interleukin-2 receptor) and CD38 on phytohaemagglutinin-activated lymphocytes Scand J Immunol...

Ngày tải lên: 09/08/2014, 08:23

... of Figure Apoptosis induction by isoproterenol or propranolol in combination with irradiation in BGC-823 and SGC-7901 cells There was an increasing rate of apoptosis in gastric cancer cell lines ... activity in cancer cells, thus making the cells radioresistant [12] Activation of NF-B by various stimuli, including inflammation, stress and radiation, involves degradation of the inhibitory ... protein level in both cell lines Figure shows that expression of b1- and b2adrenergic receptors in SGC-7901 cells was higher than that in BGC-823 cells Dose-survival curves of gastric cancer cells...

Ngày tải lên: 09/08/2014, 09:20

... induced by goserelin incubation and irradiation in LNCaP cells In PC-3 cells, we found more expression of this protein after incubation with goserelin than after irradiation alone Goserelin incubation ... effects of goserelin on irradiated PC cell lines in vitro The clinically observed increase in tumor control after combination of goserelin with radiotherapy in PC cannot be attributed to an increase ... moderate induction of apoptosis by irradiation in this cell line (see discussion) leading to an insignificant reduction in the number of viable cells PC-3 Also in PC-3 cells an exponential growth kinetic...

Ngày tải lên: 09/08/2014, 10:21

... are involved in the regulation of hypertropic differentiation The TGF-β family of growth factors and their intracellular signaling molecules are involved in chondrogenesis, including terminal differentiation ... Figure Multilineage differentiation potential of mesenchymal stem cells (MSCs) Under appropriate conditions, MSCs are able to differentiate into cell types of different lineages, including bone, ... the cytokine secretion profile of dendritic cells, naïve and effector T cells, and NK cells to induce a more anti-inflammatory or tolerant phenotype Secretion of the proinflammatory cytokines, TNF-α...

Ngày tải lên: 09/08/2014, 13:22

... and cytochemical changes in HPB-AML-I cells following the induction of differentiation toward mesenchymal lineage cells Undifferentiated HPB-AML-I cells observed with an inverted microscope are ... In vitro and in vivo immunosuppressive characteristics of hepatocyte growth factor-modified murine mesenchymal stem cells In Vivo 2009, 23:21-27 Zhi-Gang Z, Wei-Ming L, Zhi-Chao C, Yong Y, Ping ... MSCs Inverted microscopic examination (Figure 1A) and May Grünwald-Giemsa staining (Figure 1B) of HPBAML-I cells revealed that this cell line is composed of round-polygonal and spindle-like cells...

Ngày tải lên: 10/08/2014, 10:20

... role of stem cells in OSA, although there have been recent reports of the detection of circulating endothelial progenitor cells in patients [18-20] and circulating MSC in a rat model [8] Accordingly, ... mechanisms in transmigration and invasion of mesenchymal stem cells J Mol Cell Cardiol 2008, 44:1072-1084 30 Estrada R, Li N, Sarojini H, An J, Lee MJ, Wang E: Secretome from mesenchymal stem cells induces ... (right) Serum of control rats was placed in the lower transwell compartment in both cases Bottom: Normalized indices for the migration of mesenchymal stem cells induced by the serum of control and...

Ngày tải lên: 12/08/2014, 11:22

... Concluding remarks The mechanisms underlying the possible in vivo immunomodulatory effects of MSCs remain a critical and unresolved question By comparing side by side the effects of MSCs in vitro ... apparent difficulties in recapitulating the in vitro effects in vivo, however, researchers should be cautioned and should remain critical concerning the use of MSCs for the treatment of human autoimmune ... disease; MSC, mesenchymal stem cell Page of Competing interests The authors have no competing interests Author details Department of Rheumatology, Leiden University Medical Center, Albinusdreef 2,...

Ngày tải lên: 12/08/2014, 14:21

... signal-enhancing system based on biotin in terms of a clearly lower background All antibodies were diluted in PBS containing 5% bovine serum albumin The signal was developed by incubation with ... carbon dioxide in serum-free DMEM/F12 Nutmix (DMEM/F12; Invitrogen, Karlsruhe, Germany) containing 0.1% pronase E (Sigma-Aldrich, Taufkirchen, Germany) in a spinner flask for fine mincing and digestion ... of the osteogenic lineage, as assessed by the detection of alkaline phosphatase, was more pronounced in CD166+ cells, indicating more progenitor cells in CD166+ than CD166- cells (Figure 5c,d)...

Ngày tải lên: 12/08/2014, 15:23