hemodynamics right ventricular hypertrophy and pulmonary vascular remodeling
Báo cáo y học: " Differential expression of Toll-like receptors on human alveolar macrophages and autologous peripheral monocytes" pdf
... ligand-induced TLR mRNA expression at h and 24 h post-stimulation TLR mRNA expression of unstimulated bronchoalveolar cells and monocytes was set as 1, and the TLR mRNA expression of the ligand-stimulated ... response to TLR ligands in monocytes and alveolar macrophages To assess the expression of TLR2, TLR4 and TLR9 by flow cytometry following ligand exposure, alveolar macrophages and monocytes were ... expression was restricted to macrophages and lymphocytes [23] The same study found that TLR3 and TLR5 were expressed exclusively on alveolar macrophages and that TLR2 and IL-4 expression were inversely...
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Báo cáo y học: "Strong inhibition of TNF-α production and inhibition of IL-8 and COX-2 mRNA expression in monocyte-derived macrophages by RWJ 67657, a p38 mitogen-activated protein kinase (MAPK) inhibitor" ppt
... monocytes (left panel) and macrophages, differentiatedfluorescence intensity Expression of CD14 (bold grey and and HLA-DR days (right panel), measured by flow cytometry and expressed in mean with ... fraction and on differentiated macrophages was detected by flow cytometric analysis in an Epics-Elite flow cytometer (Coulter Electronics, Mijdrecht, the Netherlands) 6, and IL-8), MMP1, MMP9, and ... SDS–PAGE gel and resolved by running at a constant 200 V and 15 W Semidry blotting was performed onto nitrocellulose membrane and immunodetection was with anti-phospho-p38 MAPK and peroxidase-anti-rabbit...
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Báo cáo khoa học: "Time Course Study of Cytokine mRNA Expression in LPS-Stimulated Porcine Alveolar Macrophages" pot
... the early (2 and h), the intermediate (14 and 24 h), and the late (48 and 72 h) periods after LPS-stimulation There was a slight increase of IL-6 expression in the early stage (2 and h) of LPS-stimulation ... Shin, Deog-Yong Lee, Young-Wook Cho and Han-Sang Yoo L-glutamine-containing RPMI 1640 (Gibco BRL, Grand Island, NY) supplemented with 10% fetal bovine serum (FBS) and 50 ㎍/ml gentamicin was added ... other hand, in kinetics studies of IL-12 p35 and IL-12 p40 expression in LPS-stimulated porcine alveolar macrophages, the highest IL-12 p35 and IL-12 p40 expression levels were detected after and...
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Báo cáo y học: "DHEA-dependent and organ-specific regulation of TNF-α mRNA expression in a murine polymicrobial sepsis and trauma model" pps
... polymicrobial sepsis and dehydroepiandrosterone treatment in a rodent model Shock 2003, 20:338-346 McGowan JE Jr, Barnes MW, Finland M: Bacteremia at Boston City Hospital: Occurrence and mortality during ... fluid regulation during and after soft tissue trauma and hemorrhagic shock in males and proestrus females Shock 2003, 20:144-148 Diodato MD, Knoferl MW, Schwacha MG, Bland KI, Chaudry IH: Gender ... WG: Metabolism and actions of dehydroepiandrosterone in humans J Steroid Biochem Mol Biol 1991, 40:599-605 Angele MK, Catania RA, Ayala A, Cioffi WG, Bland KI, Chaudry IH: Dehydroepiandrosterone:...
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báo cáo khoa học: " Increased IL-10 mRNA expression in tumorassociated macrophage correlated with late stage of lung cancer" pps
... contributions RW and ML designed and performed the experiment and prepared the manuscript HQC and JZ supervised the project YQ, SFC, XYL acquired their authorship for assistance in collecting samples and ... TAMs(0.85(0.04-4.49) )and the macrophages (Figure 4C) Immunohistochemistry To confirm whether TAMs express IL-10 and cathepsin B in protein level, NSCLC (3 late stage (ⅢA) and early stage (Ia- Ib)) were randomly ... IL-10 expression level may tune inflammatory responses and adaptive Th2 immunity, exhibit anti-inflammatory and tissue remodeling functions and thereby, to favor tumor progression[14] We demonstrated...
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Retrovirology Research BioMed Central Open Access APOBEC3G mRNA expression in exposed ppt
... statistical analysis and wrote the manuscript KJT and RHJ provided patients' samples and summary of clinical data GRT, KJT, CEO and RHJ assisted with manuscript preparation, and helped to edit ... http://www.retrovirology.com/content/6/1/23 reported[25,26] Protease and RT sequences were amplified and analyzed by a Viroseq kit (Celera Diagnostics, Alameda, CA, USA) P2, P7, P1 and P6 of gag and the entire vif gene were amplified ... follow-up and who were naive to antiretroviral therapy This study was approved by the Bioethics and Research Committee of the National Institute of Respiratory Diseases, Mexico, and all patients and...
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Tài liệu Báo cáo Y học: Uncoupling of protein-3 induces an uncontrolled uncoupling of mitochondria after expression in muscle derived L6 cells ppt
... containing the right hand portion of the adenoviral vector genome and a lox site, and the other plasmid, pAvhUCP3lx, containing the left portion of the viral genome, the UCP3 expression cassette, and a ... preferentially and abundantly in skeletal muscle and brown adipose tissue Biochem Biophys Res Comm 235, 79–82 Rolfe, D.F & Brown, G.C (1997) Cellular energy utilization and molecular origin of standard ... Stern–Volmer Equation L6 cells were cultured and transfected as described above The medium was removed and cells were washed with NaCl/Pi Cells were trypsinized and suspended in a solution containing...
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Báo cáo khoa học: Ascorbic acid-pretreated quartz enhances cyclo-oxygenase-2 expression in RAW 264.7 murine macrophages pdf
... AA-treated (qa) quartz particles at 15, 50 and 100 lgÆmL)1 Results are expressed as density ratio between each COX-2 band and the corresponding b-actin band, relative to control Values are the mean ... AA-treated (aa) aerosil particles at 15, 50 and 100 lgÆmL)1 Results are expressed as density ratio between each COX-2 band and the corresponding b-actin band, relative to control Values are the mean ... particles was 100 lgÆmL)1 (Q100, QA100 untreated and treated) Results are expressed as the density ratio between each COX-2 band and the corresponding b-actin band relative to control Values are the mean...
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báo cáo hóa học: " Hypoxia-inducible factor-1 (HIF-1) is involved in the regulation of hypoxia-stimulated expression of monocyte chemoattractant protein-1 (MCP-1/CCL2) and MCP-5 (Ccl12) in astrocytes" pdf
... up-regulation by hypoxia and cobalt chloride, and the general correlative relationship between HIF-1α and the levels of MCP-1 and MCP-5 in astrocytes Up-regulation of MCP-1 and MCP-5 by HIF-1α in ... HIF-1α+/- and HIF-1α+/+ mice (genotyping, RT-PCR and ELISA) and reviewed the manuscript DC performed western blots, EMSA and supershift assays HC conducted experiments on FHAs (RT-PCR and ELISA) ... Media and cells were harvested for MCP-5 ELISA assay, RT-PCR detection of HIF-1α and MCP-5 mRNA expression, and western blot analysis of HIF-1α, respectively For FHAs, both hypoxic treatment and...
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Báo cáo y học: "Expression of CD147 on monocytes/macrophages in rheumatoid arthritis: its potential role in monocyte accumulation and matrix metalloproteinase production" ppt
... (G-250) and were destained with buffer consisting of 20% methanol, 10% acetic acid and 70% distilled water for 30 minutes to visualize the zymogen bands The zymography gels were scanned and analyzed ... 2.09%; AS 95.84 ± 3.44%) (Fig 1a) and no marked change was seen before and after stimulation Comparison between peripheral blood and synovial fluid from RA patients and control In the RA group, the ... Expression of CD147, MMP-1 and TIMP-1 on synovium from RA patients and control The immunohistochemistry results show that the immunoreactivity of CD147 and MMP-1 was more intense and more widespread...
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Báo cáo y học: "Identification of intra-group, inter-individual, and gene-specific variances in mRNA expression profiles in the rheumatoid arthritis synovial membrane" pot
... reading of the manuscript and Bärbel Ukena, Ulrike Körner, and Ildiko Toth for excellent technical assistance We are grateful to Andreas Roth, Rando Winter, Renée Fuhrmann, and Rudolf-Albrecht Venbrocks ... [74,75] and vascular permeability [78], as well as B cell-dependent auto-immunity and inflammation [79] The latter represents the elevated activity status of B cells (including cytokine production and ... affected pathways (and the respective genes) reported here may provide the basis for further analyses of the RA pathogenesis and the differences between RA and OA on a cellular and molecular level...
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Báo cáo y học: " Early Heat shock protein and heat shock factor 1 expression and localization in vaccinia virus infected human monocyte derived macrophage" ppt
... the cells and mean values of at least 100 cells randomly selected on each sample were 24% and 46% for infected and control cells respectively HSP70 protein increases during infection and transiently ... cDNA and 37 µl sterile water Reactions were carried out at the following conditions: 94°C for min, 60°C for min, and 72°C for 1.5 for 30 cycles (hsp70 and hsf1) or 95°C for min, 50°C for min, and ... cell protein and nucleic acids synthesis inhibition, depending on time and an infectious dose Despite the observed shutdown of host transcriptional and translational mechanisms and selective...
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Báo cáo y học: "Collagen V-induced nasal tolerance downregulates pulmonary collagen mRNA gene and TGF-beta expression in experimental systemic sclerosis" doc
... collagen V nasal tolerance didnt allow the progression of the fibrotic process and the pulmonary and vascular remodeling, and our results now confirm the therapeutic importance of collagen V role ... scleroderma model and nasal tolerance and the immunofluorescence experiments, and drafted the manuscript; WRT contributed to conception and design of the original study and analysis and interpretation ... skin remodeling of animals with SSc [18], in the present study we evaluated the amount of collagen deposition, mRNA collagen synthesis and TGF-beta expression in pulmonary septal and bronchovascular...
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Báo cáo y học: "Differential gene expression in HIV/SIV-associated and spontaneous lymphomas"
... normal human T-lymphocytes and the Jurkat T-cell line Expression of set, SMG-1, and ND4 was unchanged, and pub and a-myb were not transcribed in normal human T-lymphocytes and the Jurkat cells (data ... HIV-associated and monkey SIV-associated lymphomas and seemed to be common for virus-specific lymphomagenesis in human and monkey Table Selected human HIV-associated lymphomas h1 and h2 genes overexpressed ... presented in Fig 2a, 2b and Table Figure Northern blot analysis of the transcription levels of the set, ND4, and SMG-1 genes in human spontaneous lymphomas and normal B- and Tlymphocytes (a) 32P-labeled...
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Influence of Polyferric Sulfate Coagulant on the amoA mRNA Expression of Ammonia Oxidizer in Activated Sludge
... Noda N., Tsuneda S., Hirata A and Inamori Y (2002) Community analysis of nitrifying bacteria in an advanced and compact Gappei-Johkasou by FISH and PCR-DGGE, Water Sci and Technol., 46(11-12), 105-111 ... 10 s, elongation at 72°C for 20 s, and a final extension at 72°C for After agarose gel electrophoresis, amplicon was exiced and purified using Wizard SV Gel and PCR Clean-Up System (Promega, USA) ... courses of NH4+-N and NO2+3 N in batch experiment are shown in Fig Gradual decrease in NH4+-N and increase in NO2+3 N were observed just after the beginning of the experiment in control and both runs...
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Tài liệu Báo cáo " synthesis, cloning and expression in escherichia coli of a gene coding for Mcoti-ii " ppt
... the 58 kD protein band was found in induced sample as the major band, acounted for 80% of total protein (lane 1,3,5 and 6) 58 kD Fig 10 SDS PAGE pattern of PI-17 proteins Mc Standard molecular ... Manual, University of Helsinki, Finland, (1998), pp 4-7 Hernandez, J.F., Gagnon, J., Chiche, L., Nguyen, T.M, Andrieu, J.P., Heitz, A., Trinh H.T., Pham T.T Chau., and Le Nguyen D., V Squash trypsin ... stop codon and Xho I restriction site was introduced on the end of the proposed synthetic trypsin inhibitor gene The sequences of the forward and reversed primers are shown on fig and of the...
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Tài liệu Báo cáo khoa học: Lipopolysaccharide-evoked activation of p38 and JNK leads to an increase in ICAM-1 expression in Schwann cells of sciatic nerves ppt
... cytokines and chemokines, to express major histocompatibility complex (MHC) class II molecules and adhesion molecules, and to serve as antigen-presenting cells [18–20] These chemokines and inflammatory ... for ICAM-1 and NF-200 Scale bar = 20 lm I blot and ELISA revealed that induction of ICAM-1 was substantially inhibited by U0126 (20 lm), and completely abolished by SB202190 (10 lm) and SP600125 ... antigen-presenting cells and activate T cells in vitro in an antigen-specific and MHC-restricted manner [32], especially in the presence of cytokines Natural ligands of ICAM- 1and LFA-1 are expressed...
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Tài liệu Báo cáo khoa học: Glycation of low-density lipoprotein results in the time-dependent accumulation of cholesteryl esters and apolipoprotein B-100 protein in primary human monocyte-derived macrophages docx
... (e.g Ne-carboxymethyl-lysines and Ne-carboxyethyl-lysines and pentosidine) being known to accumulate with age on tissue proteins, and at an increased rate in LDL and atherosclerotic lesions in ... manner, via scavenger receptor SR-A- and CD36-mediated processes, and that the uptake of lipid and protein occurs in synchrony Furthermore, it is shown that both lipid and modified protein accumulate ... may have significant cellular and atherogenic effects, and requires further study SR-A and CD36 have previously been reported to account for 75–90% of the uptake and degradation of acetylated...
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Tài liệu Báo cáo khoa học: Regulation of connective tissue growth factor (CTGF/CCN2) gene transcription and mRNA stability in smooth muscle cells Involvement of RhoA GTPase and p38 MAP kinase and sensitivity to actin dynamics docx
... anisomycin and FBS on actin stress fibers in SMCs and their modulation by toxin B and latrunculin B Cells were first stimulated with either S1P, anisomycin or FBS for 30 and then fixed, permeabilized and ... the indicated periods and the amount of GTP-loaded RhoA, Cdc42 and Rac1 was determined by pull-down assay as described in Materials and methods Total amount of RhoA, Cdc42 and Rac1 in the same ... containing actinomycin D (10 lgÆmL)1) and the cells were harvested after 0, 0.5, 1, and h Total RNA was purified and analyzed by northern blot hybridization and phosphorimaging densitometry The...
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Tài liệu Báo cáo khoa học: Modulation of cyclin D1 and early growth response factor-1 gene expression in interleukin-1b-treated rat smooth muscle cells by n-6 and n-3 polyunsaturated fatty acids pdf
... production and concentrations of cyclin D1, Rb and Egr-1, in order to determine how the incorporation of EPA and DHA modulate SMC proliferation This article describes the differing effects of n-3 and ... the first-strand cDNA and 300 nM primers Amplification was performed on an iCycler (Bio-Rad), according to the manufacturer’s instructions, and cycle parameters were: 50 °C (2 min) and 95 °C (10 ... with the )973 bp cyclin D1 gene promoter and either the dominant-positive or the dominant-negative Ras and Raf mutants, and the effects of incorporated n-6 and n-3 PUFAs were explored in cells treated...
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Tìm thêm: hệ việt nam nhật bản và sức hấp dẫn của tiếng nhật tại việt nam xác định các mục tiêu của chương trình xác định các nguyên tắc biên soạn khảo sát các chuẩn giảng dạy tiếng nhật từ góc độ lí thuyết và thực tiễn khảo sát chương trình đào tạo của các đơn vị đào tạo tại nhật bản xác định thời lượng học về mặt lí thuyết và thực tế tiến hành xây dựng chương trình đào tạo dành cho đối tượng không chuyên ngữ tại việt nam điều tra đối với đối tượng giảng viên và đối tượng quản lí khảo sát các chương trình đào tạo theo những bộ giáo trình tiêu biểu nội dung cụ thể cho từng kĩ năng ở từng cấp độ xác định mức độ đáp ứng về văn hoá và chuyên môn trong ct mở máy động cơ lồng sóc mở máy động cơ rôto dây quấn hệ số công suất cosp fi p2 đặc tuyến tốc độ rôto n fi p2 đặc tuyến dòng điện stato i1 fi p2 động cơ điện không đồng bộ một pha sự cần thiết phải đầu tư xây dựng nhà máy thông tin liên lạc và các dịch vụ chỉ tiêu chất lượng 9 tr 25