haematocrit right ventricular weight and left lung volume

... fluid content in lung The wet lung weight was measured by careful excision of the lung from other adjacent extraneous tissues The lung was exposed for 48 h at 180°C and the dry weight was measured ... of wet/dry lung weight ratio, due to infiltration of inflammatory cells and edema, in relation to sham-operated mice On the contrary, AM showed a significant decrease of wet/dry lung weight ratio ... (Figure 1D) and 21 days (Figure 1G) AM-treatment prevented both ECM deposition and tissue damage at 14 (Figures 1F) and 21 days (Figure 1I) Effects of AM on production and expression of TNF-a and IL-1b...

Ngày tải lên: 12/08/2014, 13:22

... histidinetagged ubiquitin (Ubi-His; lanes and 4) The cells were either treated with GW501516 (lanes and 4) or left untreated (lanes and 3) Cells were harvested after 24 h and analyzed by immunoblotting ... as is the case, for example, for the ligand-regulated turnover and ubiquitination of PPARa [35] and PPARc [34] Agonist-regulated PPARb ubiquitination and turnover has also been described in a ... transfected with pCMX-mPPARb and either treated with lM GW501516 for 24 h post-transfection or left untreated The cells were metabolically pulse-labeled with [35S]methionine and [35S]cysteine for the...

Ngày tải lên: 30/03/2014, 03:20

... studies from left to right show the results for selenoprotein M (SelM), spicule-associated protein (SAP) and silicatein (SILIC) Signals for selenoprotein M (14-kDa band on the blot) and spicule-associated ... PAGE As shown in Fig 3, the predominant band identified corresponds to a 14-kDa protein (lanes a and b) Weaker bands corresponding to sizes of 35 kDa and 23 kDa were also detected if larger amounts ... 150 200 250 the amino acids glutamine, lysine and histidine at the N-terminus and the nonpolar amino acid proline and the hydroxy amino acids serine and threonine at the C-terminal border 3842...

Ngày tải lên: 30/03/2014, 20:20

... long and 1-2.3 µm wide (Hooper and Van Soest 2006; Leys and Degnan 2001) Embryo, larvae and post-larvae possess only one type of spicule, the oxea, being approximately 25 and 40 µm long, 0.5 and ... number and silicatein gene expression throughout A queenslandica development…………………………………………………… 59 List of Tables Table 2.1 Intron phases for A queenslandica silicatein and cathepsin L genes, and ... bones and teeth and brachiopode shells, 3) and silica, which forms the skeleton of most sponge, diatom, silicoflagellates, some choanoflagellates, chrysophytes, synurophytes and radiolarians, and...

Ngày tải lên: 13/10/2015, 14:07

... the class structure (the lower right- hand corner of the matrix), as you move towards the upper left- hand corner of the matrix (expert managers among employees, and capitalists among property ... thus be strong, well ordered and systematic, involving intensive and repeated interactions among constituent micro-elements, or weak and rather chaotic, involving few and A general framework 187 ... itself and depends upon the speci®c way in which all of the ®rms of that society are organized and interconnected All capitalist societies will have state apparatuses and private ®rms, and among...

Ngày tải lên: 01/11/2013, 07:20

... and through possession of skills and expertise If class locations de®ned in this way systematically shape the material interests and lived experiences of individuals, and if these interests and ... managers and experts On the one hand, petty bourgeois are owners of the means of production and thus have a clear stake in private property; on the other hand, they are often threatened and dominated ... class categories: comparisons of state and private sectors and of union and nonunion members Consciousness and formation 237 the difference between state and private sector middle-class employees...

Ngày tải lên: 01/11/2013, 07:20

... (PscG and PscE) and polymerizes to form the T3SS needle (B) The V antigen PcrV is released from its cytoplasmic partner (PscG) and forms the cap of the PscF needle (C) Translocator proteins PopB and ... SycD, PcrH and IpgC are shown in yellow, green and magenta, respectively The peptides located within the concave regions of PcrH and IpgC, corresponding to sections of the N-termini of PopD and IpaB, ... targeting and pore formation by the T3SS (YopB) and pathogenic Escherichia spp (EspD) all carry two predicted TM regions, and are predicted to have a N-terminal coiled-coil region and, occasionally,...

Ngày tải lên: 14/02/2014, 22:20

... the second and the third nested PCR were performed with LdEcR-F2 and LdEcR-R2 primers (52 °C) and with LdEcR-F3 and LdEcR-R3 primers (46 °C), respectively The first PCR with LdUSP-F1 and LdUSP-R1 ... structures of EcR and USP from L decemlineata (b) the analysis of mRNA expression profile of L decemlineata, EcR and USP, and (c) the measurement of the binding affinity of steroidal and nonsteroidal ... ligand binding domain (LBD, E-region) is underlined with dashes The junction of LdEcR-A and LdEcR-B1 is shown by an arrow head Gly164 and the downstream sequences are common between LdEcR-A and...

Ngày tải lên: 07/03/2014, 21:20

... Identical and similar residues within the TRP family are shown in dark and light green, those within the TTR family in dark and light blue, and those within the TLP subgroup in dark and light ... regions are found at sites corresponding to b-strands B and E in TLPs, and to a-helix E in TTRs and TRPs (B) Phylogenetic tree of TRPs, TTRs, and TLPs The tree was based on the multiple sequence ... compilation ª 2009 FEBS E Lundberg et al Stability and fibril formation of TTR and TRP A B a b a b C Fig (A) AFM images of hTTR (left) and sbTTR (right) The samples were incubated at 37 °C for 72...

Ngày tải lên: 16/03/2014, 01:20

... 2-oxoacids and 2-Oxoacid dehydrogenase complex from the Archaea pyruvate [14] In the current paper, we report the cloning and expression of the E2 and E3 genes of the same operon from T acidophilum, and ... was detected in the second peak and E3 in the third SDS ⁄ PAGE of protein from the exclusion volume peak revealed three protein bands, corresponding to E1a, E1b and E2 ⁄ E3 (the last two proteins ... restriction endonuclease digestion for E2, and NheI ⁄ EcoRI digestion for E3, and purified by gel electrophoresis and gel extraction as already described E2 and E3 genes were separately ligated into...

Ngày tải lên: 16/03/2014, 05:20

... functional and structural characterization of ETB and similar GPCRs Further analysis of the identified ETB131 and ETB93 fragments will help to identify residues involved in ligand binding and dimerization, ... Brij78, 1%; and digitonin, 0.4% Cloning procedures and protein analysis Coding regions of full-length ETB and its derivatives were amplified from cDNA by standard PCR techniques, and the fragments ... column volumes of 20 mm Hepes (pH 7.4), 150 mm NaCl and 0.02% dodecyl maltoside, and eluted with mm biotin in a total volume of mL The eluate was mixed with 25 lL of 1% sodium deoxycholate, and...

Ngày tải lên: 16/03/2014, 10:20

... Baldwin and K.S Nam 2010 Multiword Expressions Handbook of Natural Language Processing, Second Edition CRC Press, Taylor and Francis Group M -H Candito and B Crabb´ 2009 Improving gene erative statistical ... Computational Linguistics (COLING’86) J Lafferty and A McCallum and F Pereira 2001 Conditional random Fields: Probabilistic models for segmenting and labeling sequence data In Proceedings of the ... types of MWEs and the reference corpora are usually limited to certain types of MWEs Furthermore, the lexicon-driven and collocation-driven approaches not take the context into account, and therefore...

Ngày tải lên: 16/03/2014, 19:20

... FEBS L E Paraoanu and P G Layer Non-classical roles of acetylcholinesterase Fig Acetylcholinesterase as player in neurite growth and neural network formation in vivo and in vitro (Left) Formation ... neuronal cells, and also in astrocytes and fibroblasts, acetylcholinesterase may have morpho-regulatory roles There, acetylcholinesterase may form a complex with amyloid precursor protein and perlecan ... expressed in the central and peripheral nervous systems, different isoforms are found in haematopoietic cells, osteoblasts, vascular endothelial cells and leukocytes, and are induced in various...

Ngày tải lên: 23/03/2014, 07:20

... intact wild-type and ST mutant CPs can Fig Analysis of wild-type (UK3) PVX and ST mutant preparations by SDS/PAGE (8–20% gels) Lanes and 2, UK3 PVX; lanes and 4, ST mutant; lanes and 3, CPs from ... surface-located and glycosylated PVX CP N-terminal segment in preserving the structural and functional integrity of the PVX virions is discussed Materials and methods Reagents and chemicals Trypsin ... differences in their amino-acid sequences: the former has proline and isoleucine at positions and 10, respectively, and the latter has alanine and threonine (Fig 1).] The elution of the ST mutant peptide...

Ngày tải lên: 23/03/2014, 13:20

... Vector Kit and BL21 (DE3) competent cells were purchased from Novagen (Madison, WI, USA) Prestained protein molecular mass standard was from Life Technologies (Grand Island, NY, USA) Firststrand cDNA ... SULT cDNA and a sites adaptor primer as the primer pair with the first-strand cDNA as the template Amplification conditions were at 94 °C and 25 cycles of 30 s at 94 °C, 30 s at 59 °C, and at 72 ... template and oligo(dT) as the primer, the first-strand cDNA was synthesized using the First-Strand cDNA Synthesis Kit (Amersham Biosciences) Using sense (5¢-CGCGGATCC ATGAGCCGGAGAACAAGCGAAACT-3¢) and...

Ngày tải lên: 23/03/2014, 15:20

... IMAGE PROPLUS software and imported into Adobe PHOTOSHOP v5.5 (Adobe) Color images were transformed into black and white images using a grey-scale function, and brightness and contrast were adjusted ... absorption, distribution and metabolism of ethanol and its effects on nutrition and hepatic function In Medical and Social Aspects of Ethanol Abuse (Tabakoff, B., Sutker, P.B & Randall, C.L., eds), ... Localization of ADH1 and ADH4 in stomach body, pyloric region and the gastroduodenal junction by ISH and IHC analyses Detection of ADH1 mRNA in the gastric mucosa of stomach body (A) and region close...

Ngày tải lên: 23/03/2014, 17:22

... between HSP90a and HtpG and between GRP94 and HtpG On the other hand, a complex was not formed between HSP90a and GRP94 as reported previously [22] Table Interaction between the middle and C-terminal ... domain, and client binding, could be separated into the N- and C-terminal parts, respectively However, the present study demonstrates that the two regions are unable to be separated and that ... Materials Expression vector pQE9 and plasmid pREP4 were purchased from Qiagen Inc (Chatsworth, CA, USA) and expression vector pGEX4T-1, glutathione-Sepharose and low-molecular-mass markers, from...

Ngày tải lên: 23/03/2014, 20:22

... system might generate the “stem and inflection” corresponding to and his brother Viewing and and his as inflection is problematic since a mapping from the English phrase and his brother to the Arabic ... for Computational Linguistics John Lafferty, Andrew McCallum, and Fernando Pereira 2001 Conditional random fields: Probabilistic models for segmenting and labeling sequence data In Proceedings of ... such as those of Avramidis and Koehn (2008), Yeniterzi and Oflazer (2010) and others Toutanova et al.’s work showed that it is most important to model target side coherence and our stem markup also...

Ngày tải lên: 24/03/2014, 03:20

... media and sufficient oxygen supply (Sandig et al., 2005)  Animal cells have low cell density and grow at slow kinetics (Sandig et al., 2005)  They are also high sensitive mechanical stress (Sandig ... acids, vitamin and salt (Xie et al., 2003) Serum is one of important components in cell culture media providing growth and adhesion factors, low molecular -weight nutrients, hormones, and growth factors ... transgene in a functional and nonrearranged form Continuous growth in culture, cell handling, and media manipulations could affect the stability of the inserted sequence (Wurm and Schiffmann, 1999)...

Ngày tải lên: 28/03/2014, 09:20

... minimization using cns [57] Cavity volume calculations and display Cavity volumes within Par j and Par j were computed ˚ with voidoo software [58] using a probe radius of 1.4 A, and were visualized with ... fluorescence was monitored with excitation and emission wavelengths at 275 and 310 nm, with and nm bandwidth, respectively Buffer contributions were corrected and inner filter effect was negligible, ... j and substituted by a lysine in Par j 2, whereas Tyr81 is absent in both Parietaria sequences The cavity of maize ns-LTP is highly polarized and mainly hydrophobic on one side, and polar and...

Ngày tải lên: 30/03/2014, 02:20