guiding principles of the philippine lasallian family

... data). Therefore, in attempt to provide the first elements towards the understanding of the functionality of the potentially valuable, heat stable GH-57 enzymes, especially that of the T. hydrothermalis ... and, in the case of equal lengt hs, a lphabe tically. The division is based on the evolutionary tree s (Fig. 4). For the amylopullulanase from Thermococcus hydrothermalis (Q9Y8I8_THEHY), the numbering ... by the fact that the reactivation of the enzymes could be achieved by the addition of an external nucleophile to the reaction medium. Gratifyingly, in T. hydrothermalis amylopullulanase, the...

Ngày tải lên: 19/02/2014, 12:20

... ancestor of life. With respect to the studied enzymes of the POP family, POP seems to be the most conserved enzyme. Ten conserved amino acids were found at the active site of the enzyme of each of the ... proposed that the b-propeller of P. furiosus POP does not perform the same function as the mammalian enzyme, i.e. the exclusion of large peptides from the active site [25]. Clearly the role of the b-propeller has ... the POP family and a member of the serine protease family S28. The NJPLOT program was used to display the constructed phylogenetic tree. The phylogenetic trees were also con- structed using the...

Ngày tải lên: 19/02/2014, 13:20

... evolution and function of the hormonal system. We first cloned the genes of the CYP11B family of the guinea pig. The 11b- hydroxylase of the guinea pig showed higher substrate specificity than the aldosterone ... Thus, the proteins of the CYP11B subfamily catalysing the last biosynthetic steps are the key enzymes for the synthesis of both mineralocorticoids and glucocorticoids. From molecular cloning of the ... with the size of the C17 substituent of the substrate. Furthermore, analyses with the electron transfer protein adrenodoxin indicate dif- ferential sensitivity of CYP11B1 and CYP11B2 as well as the three...

Ngày tải lên: 22/02/2014, 07:20

... of the structure of NADP + -bound FeR revealed that the nicotinamide moiety of NADP + faces the re-side of the isoalloxazine ring, and that the 2¢-P-AMP moiety is held in the groove between the ... direction along the b10 of the other mono- mer, forming an anti-parallel intermolecular b sheet (Fig. 2A). The subsequent residues of the loop (Ala7– Pro13) turn into the interior of the dimer interface, and ... fulgidus FeR is one of the candidates for the ferric ion-binding residue based on the structure of the mercury-bound derivative, although a ferric ion was not found at the site of either the native or...

Ngày tải lên: 07/03/2014, 02:20

... FEBS To analyze the contribution of protein domains to the ER anchorage, the localization of the full-length enzyme was compared with those of five truncated forms. The pattern of the full-length ... enzymes. The most significant difference between the amphi- oxus and the other retinal reductases was, nevertheless, their preference for the NADH cofactor. To our knowledge, these are the first ... [29]. The overall analysis, examining the topology of the phylogenetic tree and the position of each gene inside the cluster, was informative regard- ing the orthology relationships of the distinct...

Ngày tải lên: 07/03/2014, 09:20

... between the hydrolases and transglycosi- dases, but rather there may be one between the compact cluster of Bacillus and Thermoanaerobacter CGTases and the remaining enzymes. The positions of the ... separates the amylase group from the CGTase group, while the thin dotted line indicates the change of the border between the two parts in the C domain tree and the SBD tree. Ó FEBS 2003 a-Amylase family ... separate evolution of this domain [30]. This together with the findings of the present study indicates a separate evolution of the domains C and E compared to the domains A and B. The recent introduction...

Ngày tải lên: 08/03/2014, 08:20

... inhibition of BACE1 will not adversely affect the potentially important signaling role of the ICDs released by the APP family of proteins, but may impact on other functions of this family of proteins. Experimental ... is the b-site APP-cleaving enzyme 1 (BACE1). Here, we investigated the effects of genetic manipulation of BACE1 on the processing of the APP family of proteins. BACE1 expression regulated the ... charac- terize the impact of BACE1 inhibition as a putative therapy for the treatment of AD. We found that the de novo production and the endogenous levels of ICDs were not reduced by deletion of BACE1....

Ngày tải lên: 15/03/2014, 10:20

... glutamate-induced excitotoxicity. BNIP3 is a BH3-only proapoptotic member of the Bcl-2 family. However, unlike in other members of the Bcl-2 family, the BH3 domain of BNIP3 is not required for its death-inducing activity. ... for the kainate receptor (a subtype of the ionotrophic glutamate recep- tor) that mimics the effect of glutamate. Of the 15 rats that received KA injections, five were used for prepa- ration of ... areas adjacent to the site of injection (Fig. 1A). High levels of BNIP3 immuno- staining were found in the cytoplasm of striatal neurons affected by the KA, and almost all of the BNIP3- positive...

Ngày tải lên: 22/03/2014, 17:20

... (NW_001834409:c1450413–1449631). The numbers above the boxes indicate the size of the exons, and those below the lines indicate the size of the introns. (B) Multiple amino acid sequence alignment of the RNase j family ... organization of the Cc RNase gene and its derived mRNA isoforms. The rectangles represent the exons and the horizon- tal bold lines the introns. The black parts of the rectangles show the protein-coding ... (AAUAAA). The first signal (AAUAUA) is located just 19 bp upstream of the poly(A) tail of the shorter transcript and the second one is located 46 bp upstream of the poly(A) tail of the alternative transcript....

Ngày tải lên: 23/03/2014, 06:20

... in the brain, suggesting that the counter-regulation of another im- portin a isoform may compensate for the lack of a single isoform in vivo in mammals [21]. Therefore, to determine whether the ... wild-type ovaries. The graphs represent the impa5 ) ⁄ ) ⁄ wild-type ratio for the amount of each mRNA. The data are expressed as the mean copies of each mRNA per the mRNA levels of the housekeeping ... harm the implanting embryos. Furthermore, the number of live pups born to impa5 ) ⁄ ) females was decreased, probably because of incomplete delivery of some pups. On the other hand, as all of the...

Ngày tải lên: 28/03/2014, 23:20

... from the thermophilic cyanobacteria as long as the 33-kDa protein is present. On the other hand, the full binding and functioning of the 12-kDa protein and cyt c-550 requires the presence of the ... across the chloroplast envelope and the other for transfer into the lumen of the thylakoid membrane. This indicates that the gene is located in the nuclear genome of the red alga, consistent with the ... composed of a cluster of four manganese atoms located close to the luminal surface of the transmembrane domain and coordinated mainly by amino acids of the D1 protein [1–3]. The extrinsic domain of the...

Ngày tải lên: 31/03/2014, 07:20

... predicts that they lack the PEST sequence, a protein degradation signal [40]. Based on the results of our analyses of four members of the BTG/Tob family, we propose that the C-terminal region of this family ... The life spans of the C-terminal truncated mutants were much longer than those of the full-length and the N-terminal mutants with truncation of the BTG/Tob homology domain. The BTG/Tob family members ... J.P. & Rouault, J.P. (1997) Cloning of the mouse BTG3 gene and definition of a new gene family (the BTG family) involved in the negative control of the cell cycle. Leukemia 11, 370– 375. 10....

Ngày tải lên: 31/03/2014, 21:21

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