gsh gssg ratio following h2o2 treatment

... were plated onto selective LB plates and incubated at 37°C overnight Electroporation was performed using 0.2 mm electroporation cuvettes with Gene pulser-Xcell (BioRad) 2.7.2 Molecular cloning Standard ... confers great flexibility in response to activity-dependent processes, developmental regulated alterations, neuronal diseases and post-injury recoveries Although a wide range of events lead to neuronal ... existing neuronal processes can be achieved either by retraction (Bagri, et al., 2003) or local degeneration (Watts et al., 2003), or both (Williams and Truman, 2005a; Koirala and Ko, 2004) The scale...

Ngày tải lên: 14/09/2015, 08:38

... appear to regulate the trafficking and maturation of the APP family of b-Secretase processing of APLP1 and APLP2 proteins, but BACE1 ablation did not prevent generation of APP, APLP1 and APLP2 ICDs ... [14] Animal care and handling were performed according to the Declaration of Helsinki and approved by local ethical committees Preparation of mouse brain extracts Hemibrains were homogenized in five ... identity of the enzymes involved, ectodomain shedding of APP, APLP1 and APLP2 results in the generation of membrane-bound C-terminal fragments (CTFs) These CTFs are further processed by c-secretase,...

Ngày tải lên: 15/03/2014, 10:20

... lesser degree than the parent viruses as evidenced by the uniform absence of replication one week following inoculation This is also consistent with the slightly impaired replication kinetics observed ... 97(10):5334-5339 Kern ER, Rybak RJ, Hartline CB, Bidanset DJ: Predictive efficacy of SCID-hu mouse models for treatment of human cytomegalovirus infections Antivir Chem Chemother 2001, 12 Suppl 1:149-156 Page...

Ngày tải lên: 20/06/2014, 01:20

... While pretreatment with heparinase alone reduced percent transduction an average of 26.6 % (40.4 ± 4.5 % transduction after heparinase treatment vs 55.1 ± 1.8 % control infection), pretreatment ... http://www.virologyj.com/content/3/1/29 Figure III treatment efficiently removes heparan sulfate from human tracheal epithelial (HTE) cell membranes Heparinase Heparinase III treatment efficiently removes heparan ... [2] Cells did not demonstrate morphologic signs of cell toxicity during or after treatment After one hour of enzyme treatment, cells were washed, then incubated with Cy3conjugated mouse anti-heparan...

Ngày tải lên: 20/06/2014, 01:20

... modifier mutation is retinal degeneration (Pdebrd1), which is car- ried by the C3H/HeSnJ strain but not C57BL/6EiJ, and causes progressive and selective degeneration of photoreceptor cells [18,19]; ... illumination intensities did not detect any masking defects [11] Previous analyses investigating alterations in locomotor and masking behaviors used mice carrying the Egfrwa2 allele on a mixed genetic ... of the Egfrwa2 congenic lines involved backcrossing the Egfrwa2 allele for greater than ten generations to C57BL/6J and 129S1/SvImJ genetic backgrounds The removal of the linked Wnt3avt hypomorphic...

Ngày tải lên: 10/08/2014, 09:20

... the electrophysiological effect of tolbutamide using the whole cell configuration of the patch clamp (Fig 4B.C) Concentrations of tolbutamide (100 lM) known to act selectively by binding to SUR1 ... supplemented with ATP and ADP, and pH was re-adjusted to 7.2 (see figure legends for nucleotide concentrations used in each stable line) Data analysis Radioligand binding experiments were fitted by the ... Kd) where y is the bound specific radioligand (pmolÆmg protein)1) and x is the radioligand concentration For dose–response curves with inhibition of current by tolbutamide the data from individual...

Ngày tải lên: 31/03/2014, 08:20

... described [14] The hTNFtg and JNK1-/- strains were intercrossed to obtain double mutant animals F2 generations were used and all data were generated from littermates A total of 35 mice (wt, n = 7; hTNFtg, ... in PBS containing 20% rabbit serum and were then incubated for hour at room temperature with the following antibodies (Abs): rat monoclonal antimacrophage (F4/80) Ab (Serotec Inc, Oxford, UK); ... between hTNFtg and JNK1-/-hTNFtg mice In contrast, wt and JNK1-/- animals revealed virtually no alterations of articular cartilage (Fig 5b) This suggests that TNF-α induces degradation of cartilage...

Ngày tải lên: 09/08/2014, 06:22

... levels of viral entry, HIV-1 cDNA production, nuclear import of the preintegration complex, as well as the frequency of integration into the host genome, were similar in both species [3] In contrast, ... either or 50 ng p24 per × 106 cells) and washed extensively the following day Productive infection was followed by the concentration of p24 antigen in culture supernatants Neither on day p.i ... transcripT-cellsactivityhCD4/hCCR5/hCycT1-triple-tgfollowingnot T-cells from hCD4/hCCR5/hCycT1-triple-tg rats not release virions into the culture supernatant following infection by several R5 HIV-1 strains,...

Ngày tải lên: 13/08/2014, 05:21

... Partial restoration of oxidative folding To study whether improved protein folding of the revertants accounted for the increase in gp120 incorporation into virions, we monitored Env maturation by ... the relative content of Env molecules on virions, expressed as the gp120/CA-p24 ratio (fig 2B) The gp120/ CA-p24 ratio for wt virions was arbitrarily set at 100% As anticipated, mut accumulated ... state of gp120 We compared maturation kinetics using three read-outs that we developed before (fig 3)[18] First, we analyzed the formation of disulfide bonds by following mobility changes of cellular...

Ngày tải lên: 13/08/2014, 06:20

Ngày tải lên: 13/08/2014, 20:21

... experimental setup as presented in D, however with different concentrations of the indicated CK-II inhibitors added during media change following transfection Means and standard deviations are calculated ... T-cells was performed using the DMRIE-C reagent (Invitrogen, Gibco) following manufacturer’s instructions Furthermore, electroporation of Jurkat T-cells with proviral constructs and tetherin expression ... post electroporation the percentage of GFP+ cells as well as p24 contents of the supernatants were quantified Presented are means and standard deviations from triplicate electroporations from...

Ngày tải lên: 12/08/2014, 23:22

... size-exclusion filtration, and Western blot analysis showed that the podoplanin-Fc preparations contained roughly comparable amounts of protein (Fig 3B), while the Fc-control protein preparation was ... outer primers and combined with the following inner primers:5'-GATGGAAAAGGAGCCATGAATTGTGC-3' (sense) and 5'-AGCACAATTCATGGCTCCTTTTCCAT-3' (antisense) for generation of mutant CLEC-2 N192A, 5'-TTGAGTTTTTGGCCGATGGAAAAGG-3' ... supplemented with 10% FCS and antibiotics or stimulated with PHA (Sigma) at a concentration of μg/ml and IL-2 (Roche) at a concentration of 10 U/ml Plasmids The NL4-3-based reporter virus bearing EGFP in...

Ngày tải lên: 12/08/2014, 23:23

... accumulation of 23S and 35S pre-rRNAs results in a slight decrease in the concentration of 18S rRNA (Fig 5) The lower concentrations of mature 25S and 5.8S rRNAs detected by steady-state analysis are ... This This This study study study study study study electrophoresis on 1.3% agarose gels, following denaturation with glyoxal [40] and transferred to Hybond nylon membranes (Amersham Biosciences) ... rRNA A fraction of the 27SA2 pre-rRNA is processed at the 5¢-end by a different mechanism and, following processing at the remaining sites, gives rise to the 5.8SL (5.8S long) rRNA, which is...

Ngày tải lên: 20/02/2014, 01:20

... each electroporation sample Electroporation was performed in 0.8 mL RPMI 1640 without serum by an electric pulse of 240 V and 1500 mF (Easy Ject1 Electroporator; Equibio) Immediately following the ... of 10 lL packed protein A-Sepharose (Pharmacia Biotech) for an additional 1-h incubation Following separation of the immunoprecipitate by SDS/10% PAGE, the proteins were blotted onto a nitrocellulose ... rate of radiolabelled amino acids incorporation in logarithmically growing cells was compared to that in density-arrested or differentiated cells The incorporation rate was almost two times lower...

Ngày tải lên: 22/02/2014, 07:20

... mixture prior to AMS treatment (A) MscL F54C and (B) MscL R135C expressed in the absence or presence of L-arabinose (minus ⁄ plus YidC) ), mock treatment; A, AMS treatment; A+X, AMS treatment after ... A recent study that appeared during preparation of the current paper used a very similar SCAM approach to study the requirements for targeting and integration of MscL, but the authors used MscL ... 20 Jensen CG & Pedersen S (1994) Concentrations of 4.5S RNA and Ffh protein in Escherichia coli: the stability of Ffh protein is dependent on the concentration of 4.5S RNA J Bacteriol 176, 7148–7154...

Ngày tải lên: 07/03/2014, 02:20

... fractionated by gel filtration on a Superdex 200 column and tested by immunoblotting with anti-Lpx1p antiserum The molecular masses indicated were interpolated from a calibration curve and correspond ... affinity chromatography (Fig 5A) The protein was further purified by gel filtration on a Superdex 200 column (Fig 5B) Gel filtration indicated the propensity of Lpx1p to oligomerize in vitro, albeit ... organelle sediment from oleate induced cells was analysed on a density gradient with sucrose concentrations form 32 to 54% w ⁄ v Individual fractions were analysed for catalase activity (peroxisomal...

Ngày tải lên: 07/03/2014, 05:20

... side-effect of cell death, because this cell line does not show any detectable apoptosis after FasL treatment Using this system, we recently discovered that caspase-8-mediated cell-autonomous NF-jB ... (Fig 4D) Consistent with this, the reconstituted FasL-induced AP-1 activation was abrogated by pretreatment with Z-VAD-fmk These results indicate that the catalytic activity of caspase-8 is required ... phosphorylation of ERK (data not shown), had no effect on the FasL-induced AP-1 activation However, treatment with the JNK inhibitor SP600125 abrogated the FasL-induced AP-1 activation (Fig 5B) On...

Ngày tải lên: 07/03/2014, 09:20

... RPTPa-D2 is that of a redox sensor This function is dependent on its catalytic cysteine (Cys723) Following hydrogen peroxide-induced oxidation, this cysteine mediates the stabilization of RPTPa ... HA-RPTPa R554H was obtained by sitedirected mutagenesis using HA-RPTPa WT as the template and the following forward and reverse oligonucleotides: 5¢- ATG AAG AAG AAC CAT GTT TTA CAG ATC -3¢ and ... Biochemistry 32, 13516–13522 Buist A, Zhang YL, Keng YF, Wu L, Zhang ZY & den Hertog J (1999) Restoration of potent protein-tyrosine phosphatase activity into the membrane-distal domain of receptor...

Ngày tải lên: 15/03/2014, 10:20

... several independent preparations of thylakoids also revealed a lower Chl a ⁄ b ratio in the mutant, although the absolute numbers were different The reduced Chl a ⁄ b ratio suggests that plants ... complexes using dodecyl-b-d-maltoside and subsequent separation of the complexes using sucrose density gradient centrifugation After separation, the gradients were harvested in 0.5-mL fractions, ... compilation ª 2007 FEBS A Hansson et al Knock-out of the J subunit of PSI ΔPsaJ WT 0.7 ΔJ P700 oxidation ratio 0.6 0.5 0.4 WT 0.3 0.2 0.1 0.0 100 200 300 400 Light intensity (μE) Fig Phenotype of homoplastomic...

Ngày tải lên: 16/03/2014, 11:20

... earlier experiments using a replication-competent construct [8] It is possible that alterations in the ratios of Rep ⁄ RepA affects the ability of RepA to bind to the LIR, once again suggesting ... activities are shown for constructs (A) pPvGUS and (B) pPcGUS cobombarded into NT-1 cells along with the following BeYDV-encoded gene products: lane 1, p35SDintron; lane 2, p35SRepA; lane 3, p35Srep; lane ... illustrating the profile of replication products collected from NT-1 cells cobombarded with the following BeYDV-based constructs Lane 1, pBYDLIR-SIR, p35SRep and pBYV1V2; lane 2, pBYDLIR-SIR,...

Ngày tải lên: 16/03/2014, 13:20