growth propagation and lentiviral transduction of hes cells
Báo cáo y học: " Derivation of normal macrophages from human embryonic stem (hES) cells for applications in HIV gene therapy" pps
Ngày tải lên :
13/08/2014, 09:21
... Figure of macrophages from lentiviral vector transduced and normal hES cells Derivation Derivation of macrophages from lentiviral vector transduced and normal hES cells A) Transduced and non-transduced ... analysis of single cell suspensions of the cystic bodies showed levels of CD34 cells which ranged from 7–15% Figure 1B displays a representative FACS profile of hES- CD34 cells Purified CD34 cells ... Demonstration of HIV-1 productive infection in these cells will permit future efficacy evaluations of anti-HIV genes in this system Here we show that normal and lentiviral vector transduced hES- CD34 cells...
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Báo cáo y học: " Lentiviral transduction of Tar Decoy and CCR5 ribozyme into CD34+ progenitor cells and derivation of HIV-1 resistant T cells and macrophages" pdf
Ngày tải lên :
10/08/2014, 05:20
... http://www.aidsrestherapy.com/content/1/1/2 differentiation of end stage cells such as T cells and macrophages [15] On the contrary, lentiviral vectors appear not to have these limitations [16,17] Based on these advantages, we used ... development of viral resistance and generation of escape mutants are possible obstacles for long range efficacy of these constructs as exemplified by the recent findings of Boden et al [14] These obstacles ... highly enriched population of human CD34+ cells (>90% pure) were used for vector transductions A representative FACS profile of purified CD34+ cells is shown in Fig 2A Transduction efficiency,...
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Báo cáo y học: " Alteration of T cell immunity by lentiviral transduction of human monocyte-derived dendritic cells" pdf
Ngày tải lên :
13/08/2014, 13:20
... added and the cells were cultured at 37°C and 5% CO2 On Day 3, ml of fresh AIM-V medium containing 560 u/ml of GM-CSF and 25 ng/ml of IL-4 was added to the culture On Day 5, the non-adherent cells ... disturb the loosely adherent cells, and ml per well of new AIM-V medium containing 560 u/ml of recombinant human GM-CSF (Research Diagnostic Inc., Flanders, NJ) and 25 ng/ml of IL-4 (R&D Systems, http://www.retrovirology.com/content/1/1/37 ... percentage of positive cells and the mean fluorescence intensity (MFI) of the population were determined FACS sort of lacZ-positive cells The lentiviral siRNA vector-transduced cells co-expressing...
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GROWTH PERFORMANCE AND SPERM QUALITY OF STRESS NEGATIVE PIÉTRAIN BOARS AND THEIR HYBRIDS WITH DUROC
Ngày tải lên :
28/08/2013, 11:59
... Summer, Autumn and Winter) were the only effects included in the model Table Results of the effect of genetic group (PiDu 25, 50 75, PiCC and PiCT) and season on growth performance and semen quality ... coefficient of determination 219 Growth Performance and Sperm Quality of Stress Negative Piétrain Boars and Their Hybrids with Duroc Table Production performance, Least square means (LSMSE) of PiDu and ... Carcass composition and meat quality of crossbreds between F1(LandracexYorkshire) sows mated with LandracexDuroc (omega) and PietrainxDuroc (Pidu) Crossbred Boars Journal of Science and Developement...
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Báo cáo khoa học: The role of helix 8 and of the cytosolic C-termini in the internalization and signal transduction of B1 and B2 bradykinin receptors potx
Ngày tải lên :
07/03/2014, 16:20
... allows determination of receptor affinity shift and ligand dissociation in intact cells Biol Chem 385, 835–843 21 Marchese A, Chen C, Kim YM & Benovic JL (2003) The ins and outs of G protein-coupled ... al Results Construction of truncated and point mutated B2wts and B1 ⁄ B2 receptor chimeras Several studies with truncations of, and deletions in, the C-terminal part of B2wt have demonstrated ... 2004 FEBS A Faussner et al Role of helix and C-termini in bradykinin receptors Fig Schematic representation of the C-terminal B1wt and B2wt sequences and chimera thereof The C-terminal sequences...
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Báo cáo khoa học: Hexadecylphosphocholine inhibits phosphatidylcholine biosynthesis and the proliferation of HepG2 cells pot
Ngày tải lên :
17/03/2014, 11:20
... synthesis in HepG2 cells increasing the CT activity Thence, in another set of experiments we assayed the combined effect of HePC and oleate As it can be observed in Table 1, exposure of HepG2 cells ... amount of soluble and membrane-bound CT in the cell is to calculate the total activity of both forms for each dish (3 · 106 cells) Thus we determined the effect of 50 lM and 100 lM doses of HePC ... extracted from the cells following the procedure of Bligh and Dyer [20] The chloroform layer was dried by evaporation with a stream of nitrogen whilst the water-methanol phase was collected and used to...
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growth, structure and electrical conduction of wo3 nanorods
Ngày tải lên :
19/03/2014, 16:48
... value of the WO3 monoclinic structure represents the thickness of one monolayer These observations suggest a layer by layer growth mode In addition to the rods, there is evidence for the growth of ... proportional to the density of the rods This evidences that the formation of the nanorods results of a competition between the both 1-D and 3-D growth processes Figs and illustrate the structure ... image of the nanorod and a representation of the special current distribution It is also possible to record I(V) characteristics curve in single point mode Results 3.1 Structure and composition of...
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Báo cáo khoa học: "morphological characteristics, root growth potential and flushing response of rooted cuttings compared with transplants of Sitka spruce" pps
Ngày tải lên :
08/08/2014, 14:21
... the effects of selection and propagation Several morphological variables, root growth potential and dormancy intensity of planting stock raised from rooted cuttings were assessed and compared ... the quality of the cuttings was good, there interesting differences in morphology, RGP and flushing responses in the greenhouse tests and in the field trial, and some of these may be of operational ... G.S., Growth and morphology of rooted cuttings and seedlings of loblolly pine and their genetic analysis, in: Worrall J.L., Dinkins L., Lester D.P (Eds.), Physiology and Genetics of Reforestation,...
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báo cáo khoa học: "Upregulated expression of indoleamine 2, 3-dioxygenase in CHO cells induces apoptosis of competent T cells and increases proportion of Treg cells" pptx
Ngày tải lên :
10/08/2014, 10:21
... CHO cells possessed functional activity and could metabolize tryptophan (Figure 1D) Effect of IDO+ CHO cells on CD3+T cell apoptosis After 72 h of co-culture of CD3+T cells and IDO+ CHO cells, ... specific inhibitor of IDO in co-culture of CD3+T cells and IDO+ CHO cells, the apoptosis could not be induced (only 33.1 ± 4.87% of CD3+T cells were apoptotic) (Figure 2) Page of 10 cells co-cultured ... volume of 20 μL using 50 ng of RNA, μL of 10× PCR buffer (Takara, Japan), 10 mM of each deoxynucleoside triphosphate (dNTP), μL of each primer, 0.5 μL of Takara Taq polymerase and 13.5 μL of water...
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báo cáo khoa học: "Cellular transfer and AFM imaging of cancer cells using Bioimprint" pdf
Ngày tải lên :
11/08/2014, 00:22
... nature and response of cells, yielding insights into drug responses and effects Considerable variability in the sizes of dimple depressions and ruptures, as well as dynamic formation and grouping of ... reflects the profile of the imaging tip rather than of the rupture Further illustrating the range and variation of cell morphologies, and the potential effect the thick layer of polymer has in ... physical and chemical approach may yield a better understanding into cell functionality and mechanics Artifacts in the form of bubbles caught trapped between the cell and polymer and ripping of the...
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Radio Propagation and Remote Sensing of the Environment - Chapter 1 docx
Ngày tải lên :
11/08/2014, 21:21
... various effects and demonstrate the importance of media parameters on the appearance of these effects, the possibility of detection of that or other effects against a background of noise and other ... of amplitude, phase, frequency, and polarization and characteristics of the media (e.g., permittivity, shape) Solutions of well-posed problems provide a basis for estimation of the strength of ... Library of Congress Cataloging-in-Publication Data Armand, N A Radio propagation and remote sensing of the environment / N.A Armand, V.M Polyakov p cm Includes bibliographical references and index...
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Radio Propagation and Remote Sensing of the Environment - Chapter 2 pps
Ngày tải lên :
11/08/2014, 21:21
... assuming current density j = and density of charge ρ = It follows from these equations that vectors E and H are orthogonal to each other and to the direction of wave propagation at real vector ... Here, Φx and Φy are initial phases of the x- and y-components of the field So, amplitudes Ex and Ey can be considered as real values If we write Equation (2.45) in the view of real expressions and ... the angle of Faraday rotation is sizeable even at frequencies of hundreds of megahertz, and it should be taken into consideration when designing radio systems of this range Measurement of the plane...
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Radio Propagation and Remote Sensing of the Environment - Chapter 3 ppsx
Ngày tải lên :
11/08/2014, 21:21
... two cases of horizontal and vertical polarization In the case of horizontal polarization, the amplitudes of the reflected and refracted waves are connected linearly with the amplitude of the incident ... the case of two media with permittivities ε1 and ε3, separated by a homogeneous layer of thickness d, and with permittivity ε2 (Figure 3.2) A layer of ice floating on water is an example of such ... amplitude of these oscillations and their quasi-period depend on the layer thickness and its complex permittivity In particular, the amplitude of oscillations decreases with increased absorption and...
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Radio Propagation and Remote Sensing of the Environment - Chapter 4 potx
Ngày tải lên :
11/08/2014, 21:21
... absorption and, being multiplied by the wave number, is equal to the coefficient of extinction The separation of real and imaginary parts in Equation (4.4) leads to a pair of equations concerning ∇ψ′ and ... therefore, ordinary and extraordinary waves are practically always independent for the ultrahigh-frequency and microwave bands for the ionosphere of Earth The ray trajectories of ordinary and extraordinary ... NUMERICAL ESTIMATIONS OF ATMOSPHERIC EFFECTS Let us now turn to numerical estimation of atmospheric effects that occur during radio propagation of ultra-high-frequency and microwave bands Let us begin...
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Radio Propagation and Remote Sensing of the Environment - Chapter 5 docx
Ngày tải lên :
11/08/2014, 21:21
... Radio Propagation and Remote Sensing of the Environment volume of a medium having a single section and length ds along the direction of wave propagation The power flow density at the input of the ... σs and σa is that the cross section of scattering characterizes the spatial redistribution of the incident wave power flow, and the cross section of absorption defines the efficiency of transfer of ... vector of the jth particle, E(rj) is the complex amplitude of the summary wave incident on it, e i( j ) and g i( j ) are the vectors of direction and polarization of this wave at the point of the...
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Radio Propagation and Remote Sensing of the Environment - Chapter 6 pps
Ngày tải lên :
11/08/2014, 21:21
... problem of wave scattering by layers where both boundaries are rough A layer of ice over water or solid soil, a layer of snow on the ground, and a layer of sand on a solid base are examples of this ... Radio Propagation and Remote Sensing of the Environment One can see that the second approximation changes the coherent component of the field This change proceeds at the second order of smallness and, ... aside these special cases, we can on the surface now pay attention to examples of sea surface or extensive land areas The boundedness of scattering surfaces, in these cases, is determined, first of...
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Radio Propagation and Remote Sensing of the Environment - Chapter 7 docx
Ngày tải lên :
11/08/2014, 21:21
... 214 Thursday, September 30, 2004 1:43 PM 214 Radio Propagation and Remote Sensing of the Environment and the function (see Equations (4.78) and (4.79)): ∞ A(s) = ∫ K ( s, z ) dz = 2π ∫ K (q , ... changes to the variables: s − Wz k = s ′, z = z (the first of these two equations is the equation of characteristics) As a result of these changes, the ordinary differential equation now can be ... PM 212 Radio Propagation and Remote Sensing of the Environment ik U go s, z = U exp 2 ( ) z µ s, z dz ∫ ( ) (7.2) Here s is the vector orthogonal to the main wave propagation direction...
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Radio Propagation and Remote Sensing of the Environment - Chapter 8 pdf
Ngày tải lên :
11/08/2014, 21:21
... of the antenna, and the antenna itself is in the Fraunhofer zone of the emitting body; therefore, the field of auxiliary dipole in the area of this body coincides to a high degree of accuracy with ... the power of their fluctuations will be detected by the receiver with the weight cos η, where η is the angle between vectors e and ex In the case of statistical independence of waves of different ... Thursday, September 30, 2004 1:43 PM 224 Radio Propagation and Remote Sensing of the Environment In the case of the receiver responding only to the y-component of waves, we have: Ey 2k T = b π 2π ∫ ∫...
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Radio Propagation and Remote Sensing of the Environment - Chapter 9 pdf
Ngày tải lên :
11/08/2014, 21:21
... inclusion of the dependence of all parameters on the only coordinate counted over the ray, in the inclusion of the propagation medium permittivity, and in the label itself — the ray intensity of the ... 248 Radio Propagation and Remote Sensing of the Environment We also represented the coordinate dependence of the scattering indicatrix, emphasizing the possible types of particle changes and their ... 250 Radio Propagation and Remote Sensing of the Environment The first summand describes the soil radiation attenuated by absorption in the atmosphere, and the second one describes the sum of the...
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Radio Propagation and Remote Sensing of the Environment - Chapter 10 pptx
Ngày tải lên :
11/08/2014, 21:21
... Amplitude, intensity, and power flow of the electromagnetic field Time of propagation Direction of the radiowave propagation Phase properties of radiowaves Frequency and frequency spectrum of receiving ... bands of 0.2, 0.3, 0.8, and 1.25 cm (Figure 10.3) in the absence of clouds, snow, rain, etc One has to take into account when planning experiments the help of both aerospaceborne instruments and ... applied for the study of the atmosphere and atmospheric formations The waves of millimetric, centimetric, and decimetric bands, depending on the requirements for the sounding depth and spatial resolution,...
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