... want to live in a snake.14. My brother managed to kill the snake just at the time when I were almost exhausted. Supply the correct formofthe verbs1. Cats could fly if they (have) wings.2. ... wish our teacher (explain) would explain that lesson to us again tomorrow.11. I can hear his voice so clearly that it is as if he (be) were here but the fact is that he isn't here.12. I ... raincoat.5. If today were a holiday, I would stay in bed for all day long.6. If she made him change his mind, she would save him a lot of trouble.7. I wish he would come tomorrow.8. I wish...
... function ofthe pro -form of bone morphogenetic protein-2 (BMP-2), a member ofthe transforminggrowth factor b (TGFb) ⁄ BLP superfamily. The role ofthe 263 amino acids of the pro-peptide is currently ... tested. Interestingly, in contrast to mature BMP-2, proBMP-2did not lead to induction of AP. Instead, proBMP-2 inhibited the induction of AP by BMP-2. This result raised the question of whether ... to the ECD of BMPR-IA.Furthermore, the results indicate that both proteinsinteract with the same epitope on the ECD becauseproBMP-2 binding can be inhibited by BMP-2.To assess binding of...
... be used to distinguish between the ability of players to leverage the new Internet economy. The key criterion usedin this study isthe type of product or service a firm offers on the Internet. ... collection The primary source ofthe data usedin this study isthe 10K formofthe companies selected through Hoover’s Online, Inc. (http://www.hoovers.com). The company's Web site offers information ... years in business. t is included inthe model to control for the maturity of a company. Companies operating inthe Internet space are expected to improve their conduct of business over time. Since...
... suggested the unwinding of the single a-helix at the C-terminal portion of c, thus allowingfor unimpaired rotation ofthe remainder of c.Furthermore, the calculations suggested the very end of c ... revealed the possibility of a‘functional unwinding’ ofthe a-helix to form a swivel joint.Furthermore, they suggested a firm clamping of that part of c even without the engineered cross-link, i.e. in ... (ab)3(this work). This wouldmake the proposed unwinding ofthe a-helix in c afeatureof the wild-type enzyme and an integral element ofthe catalyticmechanism. Such a permanent immobilization of the Correspondence...
... blades inthe axisarea are of polar character. The water channel in the apo formofthe enzyme runs along the axis of the b-propeller through the whole domain and is onlyblocked by Cys236 inthe ... from the internal b-sheet ofthe b-sand-wich domain. The last four amino acids ofthe nativesequence at the C-terminus and the His-tag protrudefrom the b-sandwich domain and interact with the ... attack on the anomeric carbonof fructose. The leaving group is carboxylate of Asp54.Dimerization The asymmetric units ofthe crystals of both the apoand complexed formofthe enzyme consist of dimerswith...
... between the hypophosphorylated initiation isoform IIA and the hyperphosphorylated elongating isoform IIO thatexhibits reduced electrophoretic mobility comparedwith the IIA isoform. Whereas the ... (b) correlation of the interaction with phosphorylation of Ser5 ofthe C-terminal domain hepta-peptide repeat, and (c) the ability of C-terminal domain kinase inhibitorsto abolish it. Polymerase ... carrying out the labelling procedure withoutprimary antibody. The efficiency of blocking was controlledby performing the labelling procedure inthe absence of the second primary antibody. The...
... regulation ofthe PKIand PKI isoforms ofthe inhibitor protein ofthe cAMP-dependentprotein kinase. J. Biol. Chem. 272, 20011–20020.20. Byler, D.M. & Susi, H. (1986) Examination ofthe secondarystructure ... addi-tional isoforms ofthe endogenous protein kinase inhibitor of cAMP-dependent protein kinase in mouse. J. Biol. Chem. 268,10927–10931.10. Olsen, S.R. & Uhler, M.D. (1991) Inhibition of protein ... causesdissociation ofthe holoenzyme complex and release of two active C subunits [1,2]. In addition, there is a second level of regulation of cAPKactivity by protein kinase inhibitors (PKIs). The...
... that the cleft of the active site will be located inthe interface between the two structural domains of CPY and would be filled by the propeptide, thus uniting the two domains in a body, as if the ... peak (Fig. 1A), indicating that the thermalunfolding process ofthe precursor form follows a two-statetransition. The ratio ofthe unfolding enthalpy (DHcal)tothevan’t Hoff enthalpy (DHv) ... closedtriangles in Fig. 3B).DiscussionStructural properties of mature form (CPY)As far as can be seen inthe experiments involving the stepwise increase in temperature and pressure, the heat-induced...
... inthe unfoldingthermodynamic parameters ofthe two forms are given in Tables 1–3. Inthe entire experimental regime, it is seen that the holo form presents better stability than the other form ... (Fig. 4C). The DG of stability is on anaverage 20% greater inthe case ofthe holoprotein ascompared to the apoprotein. Similarly, the Cm of the holo form always lies above the apo form at all ... thrombin cleavage site was engineered in the pET28a vector immediately after the N-terminal His-tag. The N-terminus His-tag of ACP was cleaved by the addition of 1 U of thrombin from bovine plasma...
... follows. In Section 2 the statistical framework forming the basis of a default generating process using binomial distribution is briefly reviewed. In Section 3 we derive the probability of default ... pool consists of NY rated entities with the same rating grade at the beginning of a year Y. In our case NY denotes the number of entities rated “A” at the beginning of year Y. The cohort ... Within this context the paper proposes a simple mechanism for the comparison of the performance of major rating agencies and that of other credit assessment systems, such as the internal ratings-based...
... work inthe elds of aesthetics and the philosophy of art; building diversity and inclusiveness in these elds; raising the prole of aesthetics and the philosophy of art within the profession ... neuroaesthetics. This new branch of empirical aesthetics is often dened as the study ofthe neural processes underlying aesthetic experience. In other words, the job of neuroaestheticians is to discover ... implicit tracing ofthe draftsman’s hand in drawings to an embodied listening in audiovisual works or an explicit feeling of co-embodiment in dance or theatre per-formances. The notion of ‘inner...
... treatment of microsomes containingCLIC4 results in a 27 kDa reduction inthe size of the protein, leaving a 6 kDa fragment [4]. This supports the hypothesis that the integral membrane formof the ... C-terminus,while Arg206 is on the top ofthe molecule, inthe loopleaving helix 6 and it points inthe opposite directionto the other basic residues (Fig. 2B). The structure of the NLS is almost ... to the side chain of Asn81. The intervening residues ofthe C-terminalextension interact with the neighboring monomer viahydrophobic contacts.Comparison with the structure ofthe solubleform...
... parsing models use linguisticintuitions to find this minimal set, for example byrestricting the statistical dependencies to the locality of headwords of constituents (Collins1997, 1999; Eisner ... be the case that DOP's gain in parseaccuracy with increasing subtree depth is due to the model becoming sensitive to the influence of lexical heads higher inthe tree, and that this gaincould ... considers counts of subtrees of a wide range of sizes in computing the probability of a tree: everything from counts of single-level rules to counts of entire trees. Thismeans that the model is sensitive...
... sentences as Wine is wine (two readings, both analytic) Wine is a wine (false) All wine is wine (analytic) Claret is a wine (true) Cheap wine is a wine (false) *All wine is a wine (semantically ... "objects". In this last group we have in mind mass predicates such as is wine. is furniture, is food, and is computer software. Both of these theories take it that is wine is true of the (abstract) ... quantity of wine. (This ring is goM or This hamburger is food are clearer examples ofthe application of mass predicates to objects.) Generally speaking, the theories view the kinds of M as forming...