gold mine in the sky gene autry

... DNA-binding zinc cluster at the N-terminus, whereas the acidic activation domain is usually present at the C-terminus. The mid- dle homology region (MHR) bridging the DNA-bind- ing and the activation ... were raised in rabbits against a GST-War1p fusion protein containing 294 amino acids of the C-terminal part of War1p (amino acids 650– 944) fused in- frame to the C-terminus of GST. The gene fusion ... lack of DNA binding. Indeed, in the case of War1-42p, in vivo footprinting data (Fig. 5) indicate an inability to bind to the WARE, which is normally decorated by wild-type War1p in the presence...

Ngày tải lên: 19/02/2014, 00:20

... a proportionate increase in the intensity of the DHS fragments in the control samples. In Fig. 7A the forskolin-treated chromatin shows a relative increase in the intensity of the DHS10a (1716 ... similar intensity but the DHS in intron 18 is less evident ( Fig. 5B). In contrast, the DHS in intron 18 is more prominent than those in introns 16 and 17 (Fig. 5A) in chromatin from Capan1 cells. The ... + 10 kb in intron 1 of the CFTR gene augments intestinal expression of the gene in vivo, both in human colon carcinoma cells and in transgenic mice [15]. It is probable t hat several other DHS that...

Ngày tải lên: 08/03/2014, 10:20

Ngày tải lên: 18/06/2014, 18:20

... and mine it very deeply, not necessarily discovering every aspect of the domain but requiring that the various aspects be integrated with one another to produce a coherent whole. Even in the ... officers represented in it. The system merely made no distinction between "all" and "any", and interpreted the final query to mean the But, you are probably thinking, those examples ... THERE STILL IS GOLD IN THE DATABASE MINE Madeleine Bates BBN Laboratories 10 Moulton Street Cambridge, MA 02238 Let me state clearly at the outset that I disagree with the premise...

Ngày tải lên: 21/02/2014, 20:20

... found in the A and B subdomains. The pocket-domain is responsible for binding to proteins containing LXCXE motifs while the pocket-domain and the C-domain are involved in binding E2F proteins. ... cellular proteins. Although the binding site for LXCXE motif containing proteins is present in the B sub- domain, the crystal structure of the pRb A/BpocketboundtotheLXCXE- containing part of HPV-16 ... pRb inhibits the transcriptional activity of E2F by binding to its carboxy-terminal transactivation domain, thereby preventing the interaction of E2F with the basal transcription machinery (Helin...

Ngày tải lên: 25/10/2013, 21:20

... man- ganese in the transcriptional regulation of the nrd operon [35] may not be considered in the light of the results obtained in the present study. Rather, the involvement of manganese in the in vivo ... results) to the nucleotide- producer C. ammoniagenes [10]. The present strategy of reintroducing the nrdF gene into the genetic background of corynebacteria comprised an initial transfer into the accessible ... samples. The rad- ical concentration was calculated by determining the area of the radical signal in the absorption spectrum by cubic spline interpolation [52] in the range 403–413 nm using the coefficient...

Ngày tải lên: 15/02/2014, 01:20

... containing the MuvB proteins Lin-37, Lin-52, Lin-54 and chromatin- associated Lin-9 and Lin-53 ⁄ RBBP4 ⁄ RbAp48. Furthermore, A-Myb and B-Myb were present in immunoprecipitations for Lin-9, Lin-37 ... complexes of cyclin-dependent kinases (cdks) and their corresponding cyclins. A prominent example of such a pair is cyclin B and cyclin-dependent kinase 1 (cdk1 ⁄ Cdc2) controlling the checkpoint between ... appears stronger in G 2 ⁄ M than in G 1 [44]. This contradicts repression through a complex in G 1 and in vivo foot- printing results in the original definition of the sites [10]. Taken together, the sites in...

Ngày tải lên: 16/02/2014, 09:20

... results in the emission of light. The intensity of the bioluminescence was detected using a luminometer, and the mitochondrial ATP concentration was determined. Mitochondrial protein concentration ... 2007) doi:10.1111/j.1742-4658.2007.06051.x The mechanisms underlying increased cardioprotection in younger female mice are unclear. We hypothesized that serine-threonine protein kinase (protein kinase B; Akt) triggers a metabolic gene ... in fatty acid utilization, increase in glucose utilization) in the murine female heart. Moreover, our data suggest that these changes occur in an Akt-independent manner. Further studies are therefore...

Ngày tải lên: 18/02/2014, 16:20

... and the remaining DHSs in the deleted cells were examined to determine the roles of these DHSs on the transcription of Ig-b gene and on the mainten- ance of the active chromatin state. Results Generation ... determined by transient transfection, was associated with DHSs in the Na channel gene and in the first intron of the Ig-b gene. Furthermore, the acetylation status of H3 and H4 histones was examined ... in the b-globin locus are the same as the wild type control [17]. Thus, there again has been no description either of the region involved in DNase I general sensitivity or of the region for the...

Ngày tải lên: 19/02/2014, 16:20

... from them, are the true inducers of galA expression. This indicates that galA is coexpressed with both the pectinolytic genes encoding main chain cleaving enzymes as well as with the arabinanolytic genes ... synergy of enzymes degrading the pectin side chains was studied [34]. GALA had a positive effect on the activity of the other enzymes involved in the degradation of these side chains. DISCUSSION A. ... under the control of the xylanolytic activator protein [32,41]. The increase in expression levels of galA in the CREA mutant, indicates that at least one of the detected putative CREA binding sites...

Ngày tải lên: 21/02/2014, 01:21

... products in the absence of the cDNA library. Fig. 6. Transcription start sites in the integrin a subunit genes. The sequences flanking the transcription start sites in the integrin a subunit genes ... binding factors. The introduction of mutation in one of the Ets-binding sequences greatly decreased its promoter activity, suggesting that the transcription of the a3 integrin gene in these cells ... including Ets, GATA, and MyoD/E-box binding factors. The introduction of mutation into one of the putative Ets- binding sequences suppressed the promoter activity. In addition, the specific binding...

Ngày tải lên: 21/02/2014, 03:20

... intron sequences requires the introduction of 11 and 14 gaps for intron 2 and 5, respectively, as compared to 1 (intron 7) to 7 (intron 6) for the remaining introns. Finally, the 3¢ UTRs of the ... pig genes. Interestingly, we observed the greatest differences between these closely related genes within intron 2 and intron 5. This could indicate frequent recom- bination between the genes ... zone-specific biosynthesis of mineralocorticoids. In this respect it would be interesting to see whether expression of adrenodoxin is differentially regulated. To investigate the evolution of the CYP11B genes,...

Ngày tải lên: 22/02/2014, 07:20

... as STARS-interacting proteins. These novel proteins con- tain four LIM domains and a C-terminal villin head- piece domain, which mediates actin-binding in several proteins, such as villin and dematin ... occurring C-terminal 100 kDa fragment of filamin, interacting with the motor protein dynein, may exert its inhibitory effect by interfering with inter- actions between the N- and C-terminal domains, ... the AR transactivation function, and the minimal functional fragment of SV, which only contains one actin-binding site, is located in the nucleus, recruiting actin into the chromatin-remodeling complex...

Ngày tải lên: 07/03/2014, 01:20

... differences in exposure times during western blot- ting. We also observed a slight increase in SREBF1 protein level triggered in the presence of the NT siRNA, in agreement with the slight increase in SREBF1 ... out an indirect effect resulting from the silencing of the transcription factor SREBF1, which would modify gene expression in an siRNA-independent manner. Therefore, these variations in mRNA ... can generate side effects, by inducing the degradation of nontarget mRNAs sharing sequence homology with the siRNA seed region, or by repressing the translation of unintended proteins [5–10]. Indeed,...

Ngày tải lên: 07/03/2014, 05:20

... lipid-binding protein, intesti- nal 15 kDa protein, ileal bile acid-binding protein and gastrotropin, reflecting the speculations of authors on its intracellular function(s). In vitro binding assays revealed ... according to the tissue from which they were initially isolated, e.g. liver- type fatty acid-binding protein (L-FABP), brain-type fatty acid-binding protein (B-FABP), intestinal-type fatty acid-binding ... The coding sequence derived from the fabp6 gene at the Wellcome Trust Sanger Institute was derived from the Tu ¨ bingen strain of zebrafish, while the sequences for the fabp6 cDNA generated in this study...

Ngày tải lên: 07/03/2014, 06:20

... Canada Fatty acid-binding proteins (FABPs) are encoded by a multigene family termed the intracellular lipid-binding protein (iLBP) genes [1,2]. In vertebrates, at least 16 paralogous iLBPs, including 10 ... shown in upper-case letters, with the coding sequences of each exon underlined and the deduced amino acid sequence indicated below. Numbers on the right indicate nucleotide posi- tions in the gene ... nucleotide difference in the coding region of exon 2, resulting in an alteration of the ninth codon of this exon from GTT to GTC without changing the encoded amino acid. The genomic sequence and...

Ngày tải lên: 07/03/2014, 10:20

Ngày tải lên: 15/03/2014, 16:20