glial cell line derived neurotrophic factor signaling

... strain and Mammalian cell line 87 Appendix III: Gene Organization and Sequences 89 vi Summary Glial cell- line derived neurotrophic factor (GDNF) is a potent neurotrophic factor which shows restorative ... Mammalian cell culture 30 2.3.1 Mammalian cell line 30 2.3.2 Cell transfection by FuGENE 6® Transfection Reagent 31 2.3.3 Haemocytometer for cell count 31 2.3.4 Cell Proliferation measured by Cell ... neurotrophic factors include neurotrophins, neurokines and the glial cell line- derived neurotrophic (GDNF) family of ligands All these neurotrophic factors signal via specific multi-component receptor...

Ngày tải lên: 05/10/2015, 22:31

... brain -derived growth factor (BDGF) and scatter factor/ hepatocyte growth factor (SF/HGF) Glial cell line- derived neurotrophic factor (GDNF) was originally identified in 1993 by Lin et al as a neurotrophic ... gliomas 4.4 Growth Factors 4.5 Cellular Signalling 4.6 Paracrine and Autocrine Loops in Cancer 4.7 Paracrine and Autocrine Loops in Gliomas 4.8 Glial Cell Line- Derived Neurotrophic Factor (GDNF) Family ... 1.11.2 Glioma Invasiveness 1.11.3 Angiogenesis 1.11.4 Glioma Signaling Pathways and Growth Factors 1.12 Glial Cell- Line Derived Neurotrophic Factor (GDNF) Family 1.12.1 GDNF Family of Ligands (GFL)...

Ngày tải lên: 14/09/2015, 12:13

... LIST 35 iii SUMMARY Glial cell line- derived neurotrophic factor (GDNF) signaling through its receptor GFRα1 plays an important role in many biological processes including cell survival, proliferation ... with glial cell line- derived neurotrophic factor in sympathetic neurons Proc Natl Acad Sci U S A 94, 7018-23 De Rooij DG (1973) Spermatogonial stem cell renewal in the mouse I Normal situation Cell ... glial- cell- line- derived neurotrophic factor Nature 384, 467-70 Kubota H, Avarbock MR, Brinster RL (2004) Growth factors essential for self-renewal and expansion of mouse spermatogonial stem cells...

Ngày tải lên: 03/10/2015, 20:57

... microglial activation in chronic pain mouse models 1.1.2.1.1 T98G Cell Line as an in vitro Astrocytic Model The cell line used in this project is the T98G human glial cell line T98G cell line ... in turn lead to activation of glial cells that are in close proximity to the afferent neuron terminals (Vallejo et al., 2010) Glial cells, forming 70% of the total cell population in the brain ... Hence, studies on non-neuronal cells, in particularly glial cells in chronic pain conditions, have increased tremendously in recent years 1.1.2.1 Role of Glial Cells in Chronic Pain Recent studies...

Ngày tải lên: 02/10/2015, 17:15

... action of extracellular nucleotides on the human lung adenocarcinoma cell line LXF-289, which is derived from solid lung adenocarcinoma Extracellular nucleotides strongly attenuated the cell cycle ... LXF-289 cells, leading to marked arrest of the cells in the S phase This inhibition is mediated by P2Y receptors through signaling pathways involving activation of the transcription factor nuclear factor ... MAPKs extracellular signal-related kinase ⁄ (ERK1 ⁄ 2) and p38 Our findings underline the importance of extracellular nucleotides in the specific modulation of cell proliferation Results Extracellular...

Ngày tải lên: 07/03/2014, 12:20

... purchased from Promega Cell culture The human hepatocellular carcinoma cell line, QGY-7703, was kindly provided by D Lu (Dept of Neurology, Stanford University, Palo Alto, CA, USA) The cells were maintained ... containing the extracellular domain (amino acids 114–281) of the human liver cancer cell line, QGY-7703 [27], we treated the cells with increasing concentrations of TRAIL and then analysed cell viability ... CO2 in air Cell death assay The 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay was used to determine tumour cell viability Briefly, cells were plated at · 104 cells per...

Ngày tải lên: 23/03/2014, 17:22

... in a dorsal root ganglia derived cell line Eur J Biochem 268, 5489–5496 Platika, D., Boulos, M.H., Baizer, L & Fishman, M.C (1985) Neuronal traits of clonal cell lines derived by fusion of dorsal ... related to the in vivo situation than fibroblast- or kidney -derived cell lines VR1 was not endogenously expressed in F-11 cells These cells, formerly generated and characterized by Platika et al ... gel Single cell RT-PCR of F-11 cells F-11 cells were diluted in · RT first strand buffer to a concentration of cell per lL and checked under the microscope in a 96-well plate Only single cells were...

Ngày tải lên: 31/03/2014, 07:20

... min, ending at 4°C Real-time PCR The level of gene expression for nerve growth factor (NGF), brain -derived neurotrophic factor (BDNF) and neurotrophin-3 (NT-3) from NSC cultures at passages 5-7 were ... as: Sun et al.: Modulation of the major histocompatibility complex by neural stem cell- derived neurotrophic factors used for regenerative therapy in a rat model of stroke Journal of Translational ... administration of nerve growth factor (NGF) has been shown to enhance the expression of the class I major histocompatibility complex (MHC) in neurons, but not in glial cells, and decrease the expression...

Ngày tải lên: 18/06/2014, 16:20

... implications on SIN vector production in many cell and gene therapy applications Methods Cell lines and plasmids pJ6ΩBleo plasmid and 293GPG retroviral packaging cell line [48] were generous gifts from ... transfection of the 293GPG packaging cell line as previously described [10] In brief, stable producer cells were generated by co-transfection of µg FspI-linearized control SIN vector or SINCMV ... assay on SINCMV producer cells treated with sodium butyrate Histone gel assay on SINCMV producer cells treated with sodium butyrate Treatment of SINCMV retroviral producer cells with increasing...

Ngày tải lên: 20/06/2014, 01:20

... Methods Cell culture The KK1 granulosa cell line was a gift from Dr Ilpo Huhtaniemi whose laboratory developed the cell line and from Dr Deborah Segaloff who sent us the cell line The cells are ... cell line is an excellent system for these studies in that these cells exhibit granulosa cell characteristics such as gonadotropin responsiveness and inhibin expression [8] In addition, KK1 cells ... the human KGN [6] and mouse KK1 (this study) cell lines respectively, and an in vivo study that used mouse Foxl2 knockouts [7] The KK1 cell line was derived from a transgenic female mouse in which...

Ngày tải lên: 20/06/2014, 07:20

... growth factor Nature (London) 1984, 312:364-367 Saadat S., Sendtner M., and Rohrer H Ciliary neurotrophic factor induces cholinergic differentiation of rat sympathetic neurons in culture J Cell ... 50㎛(D-F), 25㎛(H,I) Immunohistochemical Localization of Nerve Growth Factor, Glial Fibrillary Acidic Protein and Ciliary Neurotrophic Factor in Mesencephalon, Rhombencephalon, and Spinal Cord of Developing ... neuronal and glial pathways Different neurotrophic factors and proteins affected neurons and glia during developmental In the former study regarding the distribution of NGF-, GFAP- and CNTF-IR cells...

Ngày tải lên: 07/08/2014, 15:20

... chordoma cell line Methods Cell lines and culture conditions The chordoma cell line U-CH1 was kindly supplied by the Chordoma Foundation in Greensboro, NC, USA U87-MG and HeLa cell lines were ... of cell lines A) Growth curves for three cell lines Black reverse triangle indicates HeLa cells, white circle indicates U87-MG glioma cells, and black circle presents chordoma origin U-CH1-N cells ... indicated at the top, where the mutation deduces Proline at 72 to Arginine Table Cell cycle distribution and DNA contents of the three cell lines Cell line G1-phase S-phase G2/M-phase DNA content*...

Ngày tải lên: 09/08/2014, 09:21

... myelination stage, and and regulated by both cell- extrinsic factors and cell- intrinsic factors Cell- intrinsic factors include olig-1, olig-2 and Sox10, which control cell fate specification, and p27Kip ... Screen after high stringency wash 2.5 Cell culture 42 The oligodendrocyte cell line OLN-93, fiberblast cell lines HEK293, COS-7 and NIH3T3, neuroblastoma cell lines Neuro-2a (N2a) and PC12, were ... in this process In this project, endogenous expression of plexin-B3 in mammalian cell lines was screened and cell lines that express endogenous plexin-B3 expression were used as useful models to...

Ngày tải lên: 03/10/2015, 20:32

... of host cells, which probably results in the carcinogenesis of liver cells Materials and methods Cell lines and isolation of RNA HepG2 cell line was bought from ATCC and HepG2.215 cell line, which ... persistently HBV expressing cell line HepG2.215 and its parent cell line, a non-HBV expressing cell line HepG2 We found that cIAP1 and cIAP2 were clearly increased in the HBV expressing cells but XIAP was ... tumor types and tumor cell lines [ 18, 32, 29, 39, 40] such as pancreatic carcinoma cells, lung cancer cells [7, 10 ], prostate cancer cells [24] and renal carcinoma cell line [28, 19, 44 ] Other...

Ngày tải lên: 02/11/2012, 11:17

... antiapoptotic factor in human hepatocellular carcinoma cells [15] Moreover, a recent study has suggested that miR-27a functions as an oncogenic miRNA in gastric adenocarcinoma cells by the direct ... from AuGCT Inc MTT assay MGC803 cells were seeded in 96-well plates with · 103 cells per well in 100 lL of cell culture medium and incubated at 37 °C for 24 h The cells were then transfected with ... overexpression in MGC803 cells (A) Western blot analysis showed that expression of IL6R was successfully suppressed by IL6R siRNA (B, C) IL6R was knocked down in MGC803 cells, and cell growth activity...

Ngày tải lên: 18/02/2014, 04:20

... (thick lines) as first step in permeabilized cells (C) Histograms show ferritin expression in nonpermeabilized (thin lines, – saponin) and permeabilized cells (thick lines, + saponin) cells cultured ... anti-(mouse Igs) Cells were acquired immediately in a FACSCalibur and analyzed using CELLQUEST Histogram shows cell surface expression of acrolein adducts in HepG2 cells cultured without (thin line, – ... For intracellular staining, cells were first permeabilized with 0.2% saponin Labeled cells were acquired in a FACSCalibur and analyzed using the CELLQUEST software (A) Histograms show cell surface...

Ngày tải lên: 19/02/2014, 16:20

... USA) into viable cells [19] The percentage of viable cells in the treated samples was estimated, taking, as 100% cellular viability, that of the internal-control nontreated cells Preparation ... polynucleotide kinase (MBI, Fermentas, MGMBH, St Leon-Rot, Germany) Cell culture and cytotoxic assay The T-lymphoblastic leukaemic cell line (CCRF-CEM) and normal human lymphocytes, obtained from peripheral ... the identification of eukaryotic elongation factor alpha (eEF1A) as a nuclear component of the CRC in T-lymphoblastic CCRF-CEM cancer cells In these cells, we found a striking relationship between...

Ngày tải lên: 21/02/2014, 00:20

... invasive activity of the cells Inhibition of cell adhesion, migration and invasion with monoclonal antibody For inhibition of cell adhesion, migration and invasion, H7721 cells were preincubated ... cell adhesion to HUVECs after treatment with insulin, LY294002 or transfection with AS-PKB cDNA HUVEC, human umbilical vein endothelial cells; UnC, untreated control H7721 cells; Ins, H7721 cells ... LY294002, H7721 cells treated with 15 lM LY294002; LY294002 + Ins, H7721 cells treated with both LY294002 and insulin; Mock, H7721 cells transfected with pcDNA3 vector; AS-PKB, H7721 cells transfected...

Ngày tải lên: 21/02/2014, 00:20

... BAF3/-gp130/LIF-R cells were similar, Hyper-CNTF but not LIF induced neuronal differentiation of rat pheochromocytoma cells (PC12) These data point to a cell- specific difference in signaling via the ... Kodak (Rochester, NJ) Cells, cytokines and antibodies PC12 and COS-7 cells (ATCC, Manassas, VA, USA), BAF/3-gp130 cells (Immunex, Seattle, WA, USA) and BAF/3-gp130/LIF-R cells [20] were grown ... PC12 cells were grown in complete media in the presence of growth factors as indicated The percentage of responsive cells characterized by neurites extending longer than twice the diameter of cell...

Ngày tải lên: 22/02/2014, 07:20

... ICN Biochemicals Cell culture RBL2H3, a mast cell line derived from rat basophilic leukemia cells, was obtained from The Japanese Cancer Research Resources Bank (Tokyo) RBL2H3 cells and FMA3 (Furth’s ... acid, and proteasome inhibitors on TPH degradation in RBL2H3 cells and on TPH phosphorylation in FMA3 cells (A) RBL2H3 cells (1 · 105 cells per well) were cultured in the presence (closed circle) ... by proteasomes in RBL2H3 cells, a serotonin producing mast cell line J Biochem (Tokyo) 127, 121–127 Nakayama, K & Nakayama, K (2001) SCF complex regulating a variety of cellular functions Exp Med...

Ngày tải lên: 08/03/2014, 16:20