giá tri trn khng gian banach

... Entry 50 1.3.2.3 Viral Replication, Assembly and Exocytosis 51 1.3.2.4 Extrinsic versus Intrinsic Lifecycle 53 1.4 Immunology of Dengue Virus Infections 56 1.4.1 ... Interestingly, dengue epidemiology in Singapore presents itself with striking differences to other South-East Asian countries, where the main burden is considered to be among children In Singapore, ... median for non-normally distributed clinical data collected on the first visit grouped by severity 127 Table 3.29: Mean for normally and median for non-normally distributed clinical data...

Ngày tải lên: 08/11/2015, 16:46

... Competing interests The authors declare that they have no competing interests Authors' contributions HK and PKD contributed equally to this work and carried out the sequencing experiments and phylogenetic ... Djibouti isolated in 1998 Although, most Asian strains in this genotype are from South East Asian countries viz Cambodia, Vietnam, Thailand and Singapore; recent isolates from China and very old strain ... within this genotype Discussion The large scale climatic and demographic changes have resulted in distribution of Aedes species in hitherto unknown areas This has led to appearance of dengue infection...

Ngày tải lên: 12/08/2014, 04:21

... centrifugation, usually at 13 000 g in a microcentrifuge, the pellet is washed in 70% ethanol to remove excess salt before briefly drying and redissolving in an appropriate buffer such as 10 mM Tris-HCl ... 6.1 Restriction enzyme cloning It is common to incorporate restriction enzyme sites into the primers used to generate the PCR products (6) When the PCR product is digested with these restriction ... restriction endonucleases This assay system measures the cleavage rate close to the end of duplex oligonucleotides The restriction endonuclease cleavage site is shown in bold Table 6.2 Restriction...

Ngày tải lên: 25/10/2013, 22:20

... isolated using Ficoll gradient centrifugation method Cells were stored at -120°C and freshly thawed for analysis following standard procedures PBMC was used as negative matrix in TIL cell spiking studies ... Peptides HLA-A2 (*0201) restricted peptides for gp100 included peptides beginning with amino acid (aa) number 154, 209 (native or 210M-modified), 280, 457, and 476 HLAA2 restricted antigenic peptide ... 2008, 6:61 Spike and recovery Assessment of spike and recovery of an analyte in biological matrix (matrix effect) is defined as the direct or indirect alteration or interference in response due...

Ngày tải lên: 18/06/2014, 15:20

... with A/chicken/Egypt/0879-NLQP/2008 (clade 2.2.1v, open triangles, n = 34 samples) or A/chicken/Egypt/NLQP-0918Q/2009 (clade 2.2.1p, black triangles, n = 29 samples) See Additional file 3, Table ... RT-qPCR for detection and differentiation of clade 2.2.1p proper (black triangles) and 2.2.1v variant HPAIV H5N1 strains (open triangles) from Egypt compared to standard H5 RT-qPCR [20] The analysis ... running the three RT-qPCRs separately indicating that the multiplex approach produced no relevant detrimental effects on amplification efficacy (data not shown) Analytical characteristics The current...

Ngày tải lên: 12/08/2014, 01:22

... extraction Nucleotide sequences of all available full length HA genes of H5N1 viruses (N = 313) were retrieved from Influenza Virus Resource [31] and were aligned using BioEdit version 7.0.1 (Ibis Biosciences, ... linearization using XhoI (New England Biolabs, Ipswich, UK), DNA concentration was determined spectrophotometrically (NanoDrop 1000, Thermo Scientific, Wilmington, DE, USA) The plasmids were then diluted ... and 45 cycles of 15 seconds at 95°C and minute at 53°C Acknowledgements We would like to thank Dr Triono Soendoro from the National Institute of Health Research and Development, Ministry of Health,...

Ngày tải lên: 12/08/2014, 04:21

... trái to phát tri n tốt; nhiên giống nhập nội nên cần có thời gian để kết luận Giống Cayenne Lâm Đồng phát tri n lâu đời Việt Nam, chống chịu bệnh héo đỏ đầu phẩm chất tốt nên phát tri n giai đoạn ... từ bị nhiễm bệnh tới biểu tri u chứng từ tuần đến tháng Nhiều bị nhiễm vườn ươm dấu hiệu bệnh, sau thời gian trồng biểu Bệnh héo đỏ đầu thường trải qua giai đoạn phát tri n Xâm nhiễm: biểu bệnh ... giống cách kích thích chồi ngủ phát tri n Các mầm bên diện nách chồi nách, chồi cuống, chồi Mỗi mầm có khả phát tri n thành chồi làm ảnh hưởng ưu ngọn, mầm bên phát tri n thành chồi thu sử dụng nhân...

Ngày tải lên: 17/11/2012, 09:47

... 10× restriction buffer and restriction enzyme, incubation to allow digestion and then agarose gel electrophoresis to visualize the restriction fragments Of course it is only useful if a restriction ... primer for sequencing an asymmetric PCR product must be complementary to the asymmetrically amplified strand so you cannot use the same primer that was used for the asymmetric amplification Solid-phase ... fragment is available Not all restriction enzymes are active in the presence of various PCR components so an additional purification step may be required Direct restriction analysis can be useful...

Ngày tải lên: 25/10/2013, 22:20

... the NNIBP residues The position of the K103 residue is close to the entrance of the pocket and contributes through its aliphatic carbons to the hydrophobic character of that part of the pocket that ... purification (1 mg to 30 mL lysate) In the chromatographic steps the ion exchange and affinity matrices POROSÒ HQ, POROSÒ S and POROSÒ HE (PerSeptive Biosystems) were used Purified protein was used ... HIV-1 RT assay which used (poly)rCÆ(oligo)dG as the template/primer is described in [23] ˚ the tri uoromethyl group is repositioned 0.2 A away and ˚ the O10 of the benzoaxine-2-one ring 0.3 A...

Ngày tải lên: 18/03/2014, 01:20

... Biotechnology 2011, 11:70 http://www.biomedcentral.com/1472-6750/11/70 and the TaqMan probe does not contribute to specificity as the probe binds to the part of the cDNA sequence that originates from ... (Figure 5) The different Tm (peak of the melting curve) in the two master mixes may probably be attributed to different composition of the buffers MiR-specific qPCR of let-7a, miR-21, miR-23a and ... RT primer and finally, a 5’ end tail (tag) that can be varied in length to optimize the Tm [15] Strictly speaking the primer is not specific as only the last - bases in the 3’ end are complementary...

Ngày tải lên: 23/03/2014, 22:20

... trái to phát tri n tốt; nhiên giống nhập nội nên cần có thời gian để kết luận Giống Cayenne Lâm Đồng phát tri n lâu đời Việt Nam, chống chịu bệnh héo đỏ đầu phẩm chất tốt nên phát tri n giai đoạn ... từ bị nhiễm bệnh tới biểu tri u chứng từ tuần đến tháng Nhiều bị nhiễm vườn ươm dấu hiệu bệnh, sau thời gian trồng biểu Bệnh héo đỏ đầu thường trải qua giai đoạn phát tri n Xâm nhiễm: biểu bệnh ... giống cách kích thích chồi ngủ phát tri n Các mầm bên diện nách chồi nách, chồi cuống, chồi Mỗi mầm có khả phát tri n thành chồi làm ảnh hưởng ưu ngọn, mầm bên phát tri n thành chồi thu sử dụng nhân...

Ngày tải lên: 25/03/2014, 01:20

... that they have no competing interests Authors' contributions JFM is the corresponding author and main contributing author of this manuscript KK contributed to primer design, the construction of ... viral RNA Stools were diluted in a 10% (w/v) Modified Eagles Medium (Gibco) The suspension was centrifuged at 10000 rpm for 10 and 200 µl supernatant was applied to the High Pure Viral nucleic extraction ... primer dimers thus ensuring only amplified product was detected Standard curves were created in triplicate using serial dilutions of plasmids containing GI/2 and GII/4 PCR products corresponding...

Ngày tải lên: 18/06/2014, 18:20

... variation of the encoded proteins [11,13] The distribution of sequences in a quasispecies clusters around a master sequence, and the "center" of the genetic distribution can be described either as the ... within an infected individual exist as a group of related but distinct variants [11,12] This distribution of sequences is common among RNA viruses and is referred to as "quasispecies" Quasispecies ... protocol is designed to yield the consensus sequence The high genomic heterogeneity of HCV may contribute to viral immune evasion [9], promote chronicity [14], and may influence the outcome of interferon...

Ngày tải lên: 19/06/2014, 08:20

... that they have no competing interests Authors' contributions JFM is the corresponding author and main contributing author of this manuscript KK contributed to primer design, the construction of ... viral RNA Stools were diluted in a 10% (w/v) Modified Eagles Medium (Gibco) The suspension was centrifuged at 10000 rpm for 10 and 200 µl supernatant was applied to the High Pure Viral nucleic extraction ... primer dimers thus ensuring only amplified product was detected Standard curves were created in triplicate using serial dilutions of plasmids containing GI/2 and GII/4 PCR products corresponding...

Ngày tải lên: 20/06/2014, 01:20

... Distribution of serotypes among confirmed dengue positive cases by multiplex RT-PCR (M-RTPCR) Dengue virus serotypes Number of patients found infected with dengue viruses (serotype-wise distribution) ... user friendly, rapid, cost effective diagnostic tool and can be utilized in many developing countries, where dengue is endemic The sensitivity and specificity of the single-tube multiplex RT-PCR ... similar results This indicates the lower sensitivity of isolation compared to RT-PCR, which may be attributed to either inactivation of virus during transportation or failure in maintenance of cold...

Ngày tải lên: 20/06/2014, 01:20

... variation of the encoded proteins [11,13] The distribution of sequences in a quasispecies clusters around a master sequence, and the "center" of the genetic distribution can be described either as the ... within an infected individual exist as a group of related but distinct variants [11,12] This distribution of sequences is common among RNA viruses and is referred to as "quasispecies" Quasispecies ... protocol is designed to yield the consensus sequence The high genomic heterogeneity of HCV may contribute to viral immune evasion [9], promote chronicity [14], and may influence the outcome of interferon...

Ngày tải lên: 20/06/2014, 04:20

... 2-oxo-Δ3-4,5,5-trimethylcylopentenylacetic acid, which is further converted to a coenzyme A derivative (4), which is again a substrate for a third involved BVMO (2-oxo-Δ3-4,5,5trimethylcylopentenylacetic ... transformations with whole cells of Acinetobacter calcoaceticus, which was finally attributed to 2-oxo-Δ3-4,5,5-trimethylcylopentenylacetic acid monooxygenase (Gagnon et al 1995a,; Grogan et al ... sites of restriction endonucleases NdeI and XhoI for cloning with the BCA-kit and a standard curve of BSA in the same buffer in a range of 2-0.005 mg/mL was used Samples were measured in triplicates...

Ngày tải lên: 21/06/2014, 02:20

Ngày tải lên: 28/07/2014, 04:20