getting married at home
Multi criteria analysis in environmental management: Selecting the best stormwater erosion and sediment control measure in Malaysian construction sites
... units at all, so no need to perform the standardisation that has been illustrated into equations and Equations and can be applied when the criteria have different units Scores of alternatives ... which relate primarily to water attenuation, water quality improvements and enhancement of amenity/ecological provision Thus, design and construction, environmental/ecological impact, operation ... quantitatively or qualitatively depending on the particular MCA method that will be applied Figure shows the format of the performance matrix The xij values are performance measures that represent...
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Lab 7.2.4 Selecting the Root Bridge
... class (if that does not work, ask the instructor) Switch>enable Remove the VLAN database information file Switch#delete flash:vlan.dat Delete filename [vlan.dat]?[Enter] Delete flash:vlan.dat? [confirm] ... nvram: complete Check that VLAN information was deleted Verify that the VLAN configuration was deleted in Step using the show vlan command If previous VLAN configuration information (other than the ... j What is the status of the link light on the blocking port? Step Verify the running configuration file on the root switch a On the switch that was changed to be...
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Selecting the right manufacturing improvement tools, elsevier (2007)
... creating an environment that brings out the best in people, the need for aligning the organization and how that might be done, the need for innovation throughout the organization, including what ... and implementation of the asset management strategy CEO Compensation: Mis-Aligning the Organization Most people would agree that getting employee personal interests aligned with corporate interests ... compensation will have on aligning the organization Most of the articles that he has read suggest that exorbitant salaries appear common, along with the equally common rationalization that, to...
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Tài liệu Lab 7.2.4 Selecting the Root Bridge docx
... configurations Step Look at the show interface VLAN options a Type show interface vlan1? b List some of the options available: _ _ Step Look at the VLAN interface information ... j What is the status of the link light on the blocking port? Step Verify the running configuration file on the root switch 3-5 CCNA 3: Switching Basics and Intermediate Routing ... a On the switch that was changed to be the root bridge, type show running-config at the privileged exec mode prompt b Is there an entry in the running configuration file that specifies the spanning...
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Tài liệu Selecting the Top n Rows in a DataTable doc
... Fill the Orders table SqlDataAdapter da = new SqlDataAdapter("SELECT * FROM Orders", ConfigurationSettings.AppSettings["Sql_ConnectString"]); DataTable dt = new DataTable(ORDERS_TABLE); da.Fill(dt); ... private const String ORDERID_FIELD = "OrderID"; private const String FREIGHT_FIELD = "Freight"; // private void DataViewTopNSelectForm_Load(object sender, ... da.Fill(dt); da.FillSchema(dt, SchemaType.Source); // Get the default view for the table and bind it to the grid dv = dt.DefaultView; dataGrid.DataSource = dv; } private void selectButton_Click(object...
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Tài liệu Báo cáo khoa học: Stefin A displaces the occluding loop of cathepsin B only by as much as required to bind to the active site cleft doc
... stefin A-cathepsin H [28] A superimposition of complexes of cathepsin B and H with stefins showed that stefin A binds to cathepsin B as deeply as stefin B does to cathepsin H To illustrate this, ... 0.91 nm [35]) to cathepsin B indicates that the extent of the shift does not affect the inhibition constants This observation suggests that the energy cost of ligand binding associated with occluding ... nitrogen before data collection Diffraction data were collected at the XRD1 workstation at Synchrotron Elletra (Trieste, Italy) and processed using hkl2000 software [39] Determination of the space...
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Tài liệu Báo cáo khoa học: Solution structure of hirsutellin A – new insights into the active site and interacting interfaces of ribotoxins docx
... aromatic ring of F126, placed close to the catalytic H113, is in a similar orientation to that in the active site of nonspecific RNases These novel architectural features lead to new electrostatic ... coordinates of these 20 conformers have been deposited in the Research Collaboratory for Structural Bioinformatics Protein Data Bank under accession number 2kaa The resulting structures satisfied ... [26,27] Another unique feature of the HtA active centre is that H42 (corresponding to H50 in a-sarcin) shows an alternative conformation in which it is pointing towards the negatively charged D40...
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Tài liệu Báo cáo khoa học: Modulation of the enzymatic efficiency of ferredoxin-NADP(H) reductase by the amino acid volume around the catalytic site pptx
... (5¢-ACTTTTGTCTA CATGTGTGGAGTGAAAGGAATGG-3¢), FNRC266A (5¢-ACTTTTGTCTACATGGCTGGACTGAAAGGAAT GG-3¢), FNRC266AL268A (5¢-ACTTTTGTCTACATGGC TGGAGCGAAAGGAATGG-3¢) or FNRG267V (5¢-ACTT TTGTCTACATGTGTGTACTGAAAGGAATGG-3¢) ... CACTTTTGTCTACATGTTGGGACTGAAAGG-3¢) or FNRC266M (5¢-GACAACACTTTTGTCTACATGATGG GACTGAAAGG-3¢) and FNRlw1 (5¢-GTAATCTATCTA CAGAATACAGGAGGGTGATA-3¢) as primers and plasmid pCV105 [63] as template In addition, ... observed that alteration of the amino acid volume decreases the catalytic efficiency, suggesting that these steric considerations may be a requirement for high catalytic efficiency The mutations did...
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Tài liệu Báo cáo khoa học: A tyrosinase with an abnormally high tyrosine hydroxylase/dopa oxidase ratio Role of the seventh histidine and accessibility to the active site docx
... dopachrome by sodium periodate ied by heating to 60 °C and exposure to a relatively high concentration (0.5%) of the chaotropic and denaturing agent SDS Note that the concentration is at least 10 times ... crystal structure data 266 ´ D Hernandez-Romero et al The only data so far available are for sweet potato (Ipomoea batata) catechol oxidase [26] The catalytic copper center is accommodated in a central ... sonication by using a Braun Labsonic sonicator for The homogenate was centrifuged at 12 000 g for min, and the supernatant was used for purification and ⁄ or enzymatic activity determinations...
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Tài liệu Báo cáo khóa học: The effect of mutations surrounding and within the active site on the catalytic activity of ricin A chain pptx
... plated out in a volume of 100 lL in 96-well plates at a density of 1.5 · 105 cellsÆmL)1 and incubated for 18 h at 37 °C Toxin dilutions (100 lL) in DMEM were added in quadruplicate and the plates ... (Protein Data Bank code 1ift) Water molecules were added to the atomic model automatically using ARP [23] at the positions of large positive peaks in the difference electron density, only at places ... h at 30 °C Cells were harvested by centrifugation at 2740 g, resuspended in 15 mL of mM sodium phosphate buffer (pH 6.5), and lysed by sonication on ice Cell debris was pelleted by centrifugation...
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Tài liệu Báo cáo khoa học: Enlarging the gas access channel to the active site renders the regulatory hydrogenase HupUV of Rhodobacter capsulatus O2 sensitive without affecting its transductory activity docx
... TCTGCTCGATCTCGC-3¢) and UV6 (5¢-GCGAGATCG AGCAGACGCCGCAGATCCGCG-3¢) to replace Ile65 from HupV with Val; UV7 (5¢-GCATTTCAACCTCCT GTTCATGCCCGATTTC-3¢) and UV8 (5¢-GAAATCG GGCATGAACAGGAGGTTGAAATGC-3¢) ... autophosphorylation in the absence of H2 The phosphate is then transfered to the response regulator HupR, which, in contrast with most response regulators, is active in the unphosphorylated state [20] ... capsulatus and R eutropha exhibit unusual biochemical features The most interesting feature is that they are O2 insensitive [14,16,18,22], and thus could offer an attractive option for applications...
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Tài liệu Báo cáo khoa học: Site-directed mutagenesis of a loop at the active site of E1 (a2b2) of the pyruvate dehydrogenase complex A possible common sequence motif docx
... reaction mixture with E1 was incubated for exactly 10 at the relevant temperature and the catalytic activity at that temperature was then determined The inactivation temperatures for all the E1 mutants ... concentrations of pyruvate from 0.2 lM to 4000 lM and the ThDP concentrations from 0.04 lM to 200 lM When determining the kinetic parameters for ThDP, the pyruvate concentration was kept at mM Data ... concentration 400 lM) after the assay mix had been incubated for 10 at 30 °C Reductive acetylation assay This measures the reductive acetylation of the free lipoyl domain with [2-14C]pyruvate at 25...
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Tài liệu Báo cáo khoa học: The unique sites in SulA protein preferentially cleaved by ATP-dependent Lon protease from Escherichia coli ppt
... Preparation of SulA3±169 SulA3±169 and MBP (15 lg each) were separately incubated at 37 °C with lg of Lon in 25 lL of 50 mM Tris/HCl, pH 8.0, containing 15 mM MgCl2, with or without mM ATP At appropriate ... cleavage sites On the other hand, degradation was very slow in the absence of ATP (Fig 3), indicating that the degradation of SulA normally occurs in an ATP-dependent manner, consistent with the ... corresponding to that in Fig The numbers in parenthesis indicate the estimated percentage yields of each peptide after 3-min, 30-min, and 3-h incubations in the presence of ATP and 3-h incubation in the...
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Tài liệu Báo cáo khoa học: "Selecting the “Right” Number of Senses Based on Clustering Criterion Functions" pdf
... the maximum value (plateau) is not reached until k values greater than 100 We have developed three methods that take as input the H2 values generated from deltaK and automatically determine the ... “operational rule” that is not based on any distributional assumptions He suggests values of 2.75 to 3.50, but also states they would need to be adjusted for different data sets We have arrived at ... the standard deviation is shaded, and note that P K2 selected the value of k that was outside of (but closest to) that region This is interpreted as being the last value of k that resulted in a...
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Tài liệu Báo cáo Y học: Mutations in the docking site for cytochrome c on the Paracoccus heme aa3 oxidase Electron entry and kinetic phases of the reaction pptx
... (4–6 lM) was incubated with mM KCN at °C for at least h Cytochrome c concentrations were varied in the range of 2–32 lM; after mixing, the reaction was followed at 550 nm (oxidation of cytochrome ... cytochrome c oxidation kinetics into the time resolution of a stopped-flow apparatus, the reaction was followed at 140 mM ionic strength (see Materials and methods) and recorded at 550 nm (oxidation of ... detergent in one of the chromatographic steps of the standard purification procedure (see Materials and methods): prior to gel filtration, the partially purified material was incubated with a large excess...
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Selecting the best anthropometric variables to characterize a population of healthy elderly persons ppt
... nursing homes (subsidised by the state) and those living in a private home (i.e., more expensive, thus accommodating persons of a higher economic level) The population was selected by random stratified ... entire dataset (fig 4), indicating that to characterize or differentiate a population, only four anthropomorphic measurements need to be determined and the population only needs to be stratified ... eigenvalues greater than unity) that are capable of explaining 94.5% of the variance and thus most of the information in the original data set The new “latent” factors are obtained by linear combination...
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Báo cáo khoa học: Binding of ATP at the active site of human pancreatic glucokinase – nucleotide-induced conformational changes with possible implications for its kinetic cooperativity doc
... and MgATP as the variable substrates (A) At mM MgATP, positive cooperativity with respect to Glc was observed (nH = 1.95 ± 0.10) (B) At a low (0.05 mM) concentration of MgATP, the cooperativity ... related to partial catalytic activation following (Mg)ATP binding at physiological concentrations of Glc Similar or possibly larger effects of ATP in promoting a catalytically competent state ... Positive cooperativity was observed with respect to Glc at mm (saturating) MgATP (nH = 1.95 ± 0.10) (Fig 7A) with an [S]0.5 value of 8.2 ± 0.3 mm However, at 0.05 mm MgATP, the cooperativity was...
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Báo cáo khoa học: The chaperone and potential mannan-binding lectin (MBL) co-receptor calreticulin interacts with MBL through the binding site for MBL-associated serine proteases pdf
... final concentration of lgÆmL)1 in carbonate buffer, pH 9.6, and the plate was incubated with shaking for 24 h, with 100 lL per well, at °C Control wells only received coating buffer (negative control ... accepted that the MASPs are activated upon binding of MBL to its targets and that this initiates activation of the complement cascade, leading to target lysis and/or opsonization Inactivation of ... MBL at its MASPbinding site The interaction of calreticulin with MBL is similar to that observed for C1q, indicating that pathogenic targets, activating the lectin or classical complement pathways,...
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Báo cáo khoa học: Interactions of imidazoline ligands with the active site of purified monoamine oxidase A potx
... kynuramine oxidation by agmatine (50% inhibition at mm in the presence of 0.3 mm kynuramine) and its very slow reduction of anaerobic MAO-A indicate that MAO-A is not a pathway for agmatine metabolism ... oxidation of this biologically active amine [35] Inhibition of MAO-A by I2BS ligands is shown here to require over 1000-fold higher concentrations than that reported in the literature for saturation ... to act as a substrate (mean dis˚ tance in the three best orientations was A) All the three possible forms of agmatine were docked into MAO-A: neutral, protonated, and diprotonated Although the...
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