geographical distribution morphological and molecular characterization

... puri, purified proteins; M, molecular weight standards For the molecular weight standards, the bands from top to bottom correspond to 116.0, 66.2, 45.0, 35.0, 25.0 and 18.4 kDa, respectively consistent ... extracts and the supernatant and precipitate fractions are shown in Fig It was found that E coli BL21(DE3) ⁄ pHYDJs and E coli BL21(DE3) ⁄ pHYDPf produced a predominant band with an apparent molecular ... standard molecular weight proteins The flow rate was set to 0.8 mLÆmin)1 The subunit molecular mass was also estimated by SDS–PAGE Characterization and comparative analyses of HYDJs and HYDBp...

Ngày tải lên: 18/02/2014, 08:20

... C6 and C9 aldehydes (and the corresponding alcohol) were detected in all the samples and, similarly to the pattern of LOX expression and activity, their levels were higher in stage I seed and ... the shelf life and the organoleptic features of hazelnut seeds, we carried out a study to characterize hazelnut lipoxygenases at the biochemical and molecular level Materials and methods Plant ... characterization To identify and characterize hazelnut lipoxygenases at the molecular level, we performed PCR experiments using hazelnut genomic DNA as template and the primers ML1 and ML2 bordering a...

Ngày tải lên: 21/02/2014, 00:20

... Corresponding pH optima and specific activities for syringaldazine and 2,6-DMP were pH 5.6 and 3.4 UÆmg protein)1 and pH 4.6 and 2.8 UÆmg protein)1, respectively In standard assay mixtures, the ... Biochemical and molecular characterization of the diphenol oxidase of Cryptococcus neoformans: identification as a laccase J Bacteriol 176, 656–664 39 Zhao, J & Kwan, H.S (1999) Characterization, molecular ... agarose/Tris borate/EDTA gels and appropriate bands excised and purified from the gel using the NucleoTrap Gel Extraction Kit (Clontech) The purified DNA was precipitated with ethanol and resuspended in 10...

Ngày tải lên: 07/03/2014, 15:20

... therefore molecular In this section the focus will be on the various discriminating morphological features and the extent to which they cut across the molecular subtypes An overview of the morphological ... immunohistochemistry for MLH1] The glands to the left are normal and show nuclear expression of both MGMT and MLH1 The gland with features of serrated adenoma and the poorly differentiated adenocarcinoma ... pathological and molecular features An exclusively molecular classification carries little meaning John Constable said that we see nothing until we truly understand, but we can also say that we understand...

Ngày tải lên: 22/03/2014, 17:20

... 5¢- and 3¢-RACE PCRs by using the outer and inner RACE primers supplied by the manufacturer and the outer and inner gene-specific primers designed from the cel7 PCR products The Cel7 outer and ... which are stacked face-to-face and are highly curved, forming convex and concave surfaces The convex and concave sheets of the b-sandwich are composed of seven b-strands Many of the side-chains ... HEA pre-PRTLI and Enterprise Ireland awards to M.G.T C.M.C and R.T are grateful for junior teaching fellowships from NUI, Galway, and postgraduate scholarships from Enterprise Ireland References...

Ngày tải lên: 23/03/2014, 13:20

... Lys56; and aD, Ala90–Lys108); (e) two short strands 4550 of an antiparallel b-sheet (b-wing) (b2, Ser74–Ser78; and b3, Glu81–Asn85); and (f) a C-terminal loop containing a short b4-strand (His115–Asn117) ... elements labels are indicated a-Helices and b-strands are colored in red and yellow, respectively The catalytic network and the Ca2+-binding residues are indicated and shown as sticks (B) The Ca2+-binding ... Effect of temperature and pH on ChPLA2 activity (A,C) and stability (B,D) ChPLA2 ( ) and PPLA2 ( ), used as controls, were tested for activity at various temperatures (A) and pH values (C), as...

Ngày tải lên: 30/03/2014, 01:20

... capacities to identify the species and strains of viral pathogens infecting crop plants and their related wild species, and to understand their distribution within a given geographical region Access ... and digested with EcoRV for 1.5 hrs at 37°C Unique bands generated by this restriction enzyme facilitate identification of single infections with EACMV and ACMV (lanes 1–3 and respectively) and ... analysis, cloning and nucleotide sequencing The studies described here demonstrate that FTA offers a simple, sensitive and specific tool appropriate for the diagnosis and molecular characterization...

Ngày tải lên: 19/06/2014, 08:20

... capacities to identify the species and strains of viral pathogens infecting crop plants and their related wild species, and to understand their distribution within a given geographical region Access ... and digested with EcoRV for 1.5 hrs at 37°C Unique bands generated by this restriction enzyme facilitate identification of single infections with EACMV and ACMV (lanes 1–3 and respectively) and ... analysis, cloning and nucleotide sequencing The studies described here demonstrate that FTA offers a simple, sensitive and specific tool appropriate for the diagnosis and molecular characterization...

Ngày tải lên: 20/06/2014, 04:20

... Kumar S, Tamura K, Nei M MEGA3: Integrated software for Molecular Evolutionary Genetics Analysis and sequence Detection and molecular characterization of infectious bronchitis virus 223 alignment ... (AF338717), Detection and molecular characterization of infectious bronchitis virus 221 GA/8077/99 (AF338718), DLD (EU589323), THA001 (DQ449628) Results Virus screening and isolation For virus ... region of S1 gene covering HVR I and HVR II were amplified and used for typing the field isolates in Thailand Although many different strains of live attenuated and inactivated vaccines have been...

Ngày tải lên: 07/08/2014, 23:22

... of all molecular methods and the experiments, performed the sequence alignments and all other genetic and molecular data analysis, captured the macroscopic, histological and SEM images and drafted ... assays - the morphological and the molecular assay - have to be regarded as complementary [12] Regarding the indistinct descriptions and the lack of current in-depth studies and molecular data ... therefore initiated histological, microscopic and molecular examinations to clarify the identity of flower organs and of existent differences between wild-type and 'budflowering' phenotypes Results In...

Ngày tải lên: 12/08/2014, 03:21

... Materials and methods Animals and sampling Skin biopsies, EDTA blood and serum samples from 38 lambs of the Norwegian White breed from two flocks were collected in May and June of the 2006 and 2007 ... 2007 grazing seasons, in the Rogaland and Vest-Agder county of Norway, respectively The lambs were 4-6 weeks old and the samples were collected between two and three weeks after the lambs were ... from EDTA blood and skin biopsies, using a DNeasy® Blood and Tissue kit (Qiagen GmbH, Page of Hilden, Germany) according to protocols provided by the DNeasy® Blood and Tissue Handbook (2006) DNA...

Ngày tải lên: 12/08/2014, 18:22

... laboratory techniques used [27] G and/ or R banding is presently used systematically and allows the detection of rearrangements modifying very slightly the size and/ or the banding profiles of the chromosomes, ... White Candidate for reproduction rcp(5;8)(p12;q21) sire + one sister March 2000 French Landrace (FL) Candidate for reproduction rcp(15;17)(q24;q21) June 2000 Crossbred (3/4 FL; 1/4 Meishan) Candidate ... wet slides and air dried Slides were treated with 0.1% Trypsin (Difco) and stained with 3% Giemsa solution to generate GTG-banding [30] Chromosomes were arranged according to the standardized...

Ngày tải lên: 14/08/2014, 13:21

... 23-30o C and 80-90% relative humidity The house dust mites primarily feed on skin scales and dander while the storage mites feed on plants and micro-organisms (Ho and Nadchatram, 1984; Andersen ... showing IgE binding to DF414 and DF1096; DF414 and BT796; and BT796, BT1313 and BT1694 176 IgE binding reactivity of mites FABP homologues tested by 55 Singaporean sera and 42 Italian sera that are ... (DC) producing IL-12 and IL-18 enhance TH1 cell development, whereas DCs expressing ICOS ligand and producing IL-10 enhance the development of TH2 and TR cells TR cells and TH2 cells develop...

Ngày tải lên: 12/09/2015, 11:08

... antisense (GSTA4) and double-stranded (GST-DS1) cDNAs 112 Figure 23 Phenotype of WT and different transgenic Arabidopsis plants expressing sense (GST-S6), antisense (GST-A4) and doublestranded (GST-DS1) ... 2003) and Arabidopsis thaliana (Ikeda-Iwai et al., 2003), and androgenesis in rye (Immonen and Anttila, 1999) and triticale (Immonen and Robinson, 2000) An accumulation of H2O2 in culture is ... expression and its regulation 5.3.3 Changes in GST expression affect stress tolerance and flowering 5.3.4 Role of GSTs in plant morphogenesis in vitro 119 120 122 124 Cloning and characterization...

Ngày tải lên: 17/09/2015, 17:19

... NRE(A+B)); and in both Box A and B, NRE(A–B–) (C) Qualitative analysis of LacZ reporter activation in the interaction of APUM-2 and APUM-7 with NRE WT and NRE mutants The iron responsive element RNA and ... human and mouse possess two; and Drosophila and Dyctiostelium have only one member [5] Recently, new studies have revealed the presence of ten, two and one homologs in Trypanosome, Plasmodium and ... positions 12, 13 and 16, similar to human and Drosophila Pumilio On the other hand, the amino acids in these positions in APUM-7 to APUM-12 are more similar to those found in yeast Puf4 and Puf5 proteins...

Ngày tải lên: 18/02/2014, 06:20

... 60, 70 and 80 °C between 190 and 260 nm with a step increase of 0.2 nm, and a bandwidth of nm Thermal stability of Rep245 was measured by incubating the protein at 60, 70 and 80 °C for 30 and then ... prim–pol domain S Prato et al Zn-binding motif and the two disulfide bonds respectively connecting the a4-helix to the b4 strand and the b9 strand to the b10 strand at the bottom of the Zn-stem loop in ... to serve as ligands for divalent cations and as tags revealing the presence of DNA polymerases in the active site [18–20] In this study, we report on the structural and functional characterization...

Ngày tải lên: 18/02/2014, 18:20

... labeled on the left, and the positions of molecular weight standards are indicated on lane FEBS Journal 274 (2007) 1524–1529 ª 2007 The Authors Journal compilation ª 2007 FEBS 1525 Characterization ... subunit The FP band is not very clear in the agarose gel, and the longest band was used to run another PCR reaction for amplification Experimental procedures Extraction, purification and crystallization ... dyed with Coomassie blue G250, and the four bands corresponding to subunits of porcine complex II were cut out and N-terminal sequenced by the Edman degradation method and amino acid analyzer in...

Ngày tải lên: 19/02/2014, 02:20

... set of enzymes involved in amino acid synthesis of Ser from Gly and Ala from Cys and conversions between Asp and Asn and between Glu and Gln [49] Serine metabolic pathways are often absent in parasitic ... residue between 58 and 59 of EhPGDH (also missing in other Type III organisms and Type IIB B anthracis) in the substrate binding domain and five–ten residues between amino acid 172 and 173 (Fig 2) ... cyanobacteria, Clostridiales, Actinomycetales and archaebacteria belong to Type I, while b- and c-proteobacteria and Bacteroidales belong to Type IIA and Type III, respectively It is worth noting...

Ngày tải lên: 19/02/2014, 13:20

... instead of 15 residues, including strand S2, between helices H1 and H2, and 84 residues instead of 10 residues between helices H2 and H3 (Fig 2) Molecular characterization of zebrafish PrP1 The ... PrP1 and human PrPC, 62% between zebrafish PrP1 and PrP2, 66% between zebrafish PrP1 and Fugu PrP1, and 57% between zebrafish PrP1 and Fugu PrP2 sequences (Fig 2) Conserved sequence motifs and amino ... genes in the fish genome as well as the specific roles and evolution of PrP and related proteins in vertebrates Results and Discussion Molecular characterization of zebrafish PrP2 The zebrafish dbEST...

Ngày tải lên: 19/02/2014, 16:20

... some sera to a 9- and a 15-kDa band We could show that the 9-kDa band in the tomato extracts reacts with a specific antibody against the LTP from cherry, Pru av and the 15-kDa band shows reactivity ... elution fractions 30–33 Lyc e with a molecular mass of 50 kDa was eluted, fractions 34–37 contained a band of 36 kDa and the fractions 38–41 a protein with a molecular mass of about 20 kDa (Fig ... Karlsruhe, Germany) and proteins were separated by SDS/ PAGE using a 10% resolving gel with 1.5-mm spacers Desired bands were excised from the gel after staining with 0.3 M CuCl2 and the protein...

Ngày tải lên: 21/02/2014, 00:20