genetically engineered mouse models of pancreatic ductal adenocarcinoma
Báo cáo khoa học: Natriuretic peptide system: an overview of studies using genetically engineered animal models doc
Ngày tải lên :
14/03/2014, 23:20
... professor of the University of Texas, whose comments and suggestions were of inestimable value for our study using GC-A knockout mice, to Professor Misono of the University of Nevada School of ... the plasma Lessons from genetically engineered animals A variety of genetically engineered mice have been generated to study the physiological function of each component of the natriuretic peptide–receptor ... regulation of blood pressure Overall, these results show the significance of the endogenous natriuretic peptide system in the maintenance of normal blood pressure Regulation of blood volume Infusion of...
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báo cáo hóa học:" Sigma-2 receptor ligands potentiate conventional chemotherapies and improve survival in models of pancreatic adenocarcinoma" ppt
Ngày tải lên :
18/06/2014, 15:20
... of pancreatic cancer cells in vitro The sigma-2 The sigma-2 ligand SV119 induces moderate apoptosis in both G0 and G1 to G2/S phase of pancreatic cancer cells in vitro The murine pancreatic adenocarcinoma ... for pancreatic adenocarciSigma-2 ligands have a high affinity for pancreatic adenocarcinoma cell lines compared to normal cell lines Representative FACS analysis of human (A.) and murine (B.) pancreatic ... receptor-ligand binding interaction and reported the Kd and Bmax values of sigma-2 receptor ligands in models of pancreatic adenocarcinoma Furthermore, we have demonstrated that sigma-2 receptor...
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Mouse models of breast cancer metastasis: Anna Fantozzi and Gerhard Christofori pdf
Ngày tải lên :
22/03/2014, 17:20
... have to be tested in vivo by proof -of- concept experiments in reliable mouse models of breast cancer metastasis In the future it will be necessary to generate mouse models that more accurately recapitulate ... expression profiling experiments with patient samples are able to modulate breast carcinogenesis in transgenic mouse models, for example in the wellcharacterized MMTV-Neu and MMTV-PyMT mouse models of ... similarity of this Page of 11 (page number not for citation purposes) Available online http://breast-cancer-research.com/content/8/4/212 Table Transgenic mouse models of breast cancer metastasis TG mouse...
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báo cáo hóa học: " Gait dynamics in mouse models of Parkinson''''s disease and Huntington''''s disease" doc
Ngày tải lên :
19/06/2014, 10:20
... of motor dysfunction, we also studied gait dynamics in the SOD1 G93A transgenic mouse model of ALS [39] to compare gait variability in mouse models of basal ganglia disease to a mouse model of ... MPTP-treated mouse Gait signals in a MPTP-treated mouse Gait signal of the right hind limb of a MPTP-treated mouse superimposed over the gait signal of the right hind limb of a saline-treated mouse ... inability of the mice to walk at all on the treadmill belt SOD1 G93A transgenic mice To compare gait variability in the MPTP and 3NP mouse models of basal ganglia disease to a mouse model of motor...
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báo cáo hóa học: " Inflammatory cytokine levels correlate with amyloid load in transgenic mouse models of Alzheimer''''s disease" potx
Ngày tải lên :
19/06/2014, 22:20
... production by brain slices from transgenic mouse models of AD at 15 months of age Cytokine production by brain slices from transgenic mouse models of AD at 15 months of age Freshly harvested brain slices ... production by brain slices from transgenic mouse models of AD at 15 months of age Cytokine production by brain slices from transgenic mouse models of AD at 15 months of age Freshly harvested brain slices ... around areas of plaque deposition [4-7] Chronic administration of ibuprofen can reduce plaque pathology and brain Aβ levels in these animal models of AD [8,9] There are numerous reports of increased...
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báo cáo hóa học: " Expression profiles for macrophage alternative activation genes in AD and in mouse models of AD" pptx
Ngày tải lên :
19/06/2014, 22:20
... expression profiles in mouse models of AD and of cerebral amyloid angiopathy Our data demonstrate that genes typical of alternative activation are clearly expressed in AD and in a mouse model of amyloid ... profile Alternative activation gene profiles in mouse models of AD Since alternative activation could be demonstrated in vitro using the expression of specific genes, we determined if mouse models ... Figure Alternative activation genes in mouse models of amyloid pathology Alternative activation genes in mouse models of amyloid pathology The transcripts of activation-related genes were measured...
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Báo cáo y học: "Epitope spreading to citrullinated antigens in mouse models of autoimmune arthritis and demyelination" pot
Ngày tải lên :
09/08/2014, 13:22
... development and evolution of ACPA responses in rodent models of RA and MS, with the objective of gaining further insights into the aetiology, evolution and potential pathogenic role of such responses ... on mouse serum collected from control and CIA mice The protocol was adapted from the manufacturer's suggested procedure, with substitution of 1:150 dilutions of mouse serum and a goat-anti -mouse ... development and/or clinical progression of RA The purpose of these studies was to characterise the development and evolution of ACPA responses in rodent models of RA and MS, to gain insights into...
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Báo cáo y học: "Somatic structural rearrangements in genetically engineered mouse mammary tumors" pptx
Ngày tải lên :
09/08/2014, 22:23
... structural rearrangements in genetically engineered mouse tumors using PE-MPS We analyzed tumors of four genotypes of mouse mammary cancer, of which two were HR-proficient and two were HR-deficient ... Schematic representation of the fusion of genes Aldh8a1 and 6330407J23Rik by deletion (d) RT-PCR product of RNA between exon of Aldh8a1 and exon of 6330407J23Rik Sequence trace of the RT-PCR product ... subtype of disease not represented by this dataset Discussion Massively parallel sequencing of tumors from mouse models of human cancer has several advantages It permits in-depth analysis of the...
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Báo cáo y học: " Pharmacological characterisation of antiinflammatory compounds in acute and chronic mouse models of cigarette smoke-induced inflammation" ppt
Ngày tải lên :
12/08/2014, 11:22
... mechanisms of action - a glucocorticoid (budesonide), a phosphodiesterase (PDE) inhibitor (roflumilast) and a statin (fluvastatin) As one of the primary functions of preclinical disease models is ... 3mg/kg Roflumilast Sham + Vehicle CS + Vehicle CS + 3mg/kg Roflumilast C * Sham + Vehicle Figure The effect of roflumilast on chronic CSE-induced inflammatory cell infiltrate After 14 weeks of CSE, ... is to assess the potential efficacy of candidate compounds, ideally one would examine the same endpoints in the models as in the clinic Typically, early proof -of- concept studies for COPD anti-inflammatory...
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Báo cáo khoa học: Three-step hydroxylation of vitamin D3 by a genetically engineered CYP105A1 doc
Ngày tải lên :
15/03/2014, 23:20
... 1a-hydroxylation Comparison of the stability of the oxygenated form of wild type and the double variants of CYP105A1 Our previous studies revealed that the double variants of CYP105A1 – R73A ⁄ R84A ... we examined the stability of the oxygenated forms of the wild-type CYP105A1 and its variants Figure shows spectral analysis of the NADPHdependent reduction of heme iron of the P450s Although the ... addition of thiostrepton, 2-hydroxypropyl-b-cyclodextrin solution (200 mgÆmL)1 of water) and vitamin D3-ethanol solution (20 mg in 0.5 mL of ethanol) were added to 100 mL of culture of the recombinant...
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SAFETY OF GENETICALLY ENGINEERED FOODS APPROACHES TO ASSESSING UNINTENDED HEALTH EFFECTS pdf
Ngày tải lên :
22/03/2014, 16:21
... Assessing Unintended Effects of Genetically Engineered Foods on Human Health Copyright © National Academy of Sciences All rights reserved Safety of Genetically Engineered Foods: Approaches to ... Academy of Sciences All rights reserved Safety of Genetically Engineered Foods: Approaches to Assessing Unintended Health Effects http://www.nap.edu/catalog/10977.html SAFETY OF GENETICALLY ENGINEERED ... Academy of Sciences All rights reserved Safety of Genetically Engineered Foods: Approaches to Assessing Unintended Health Effects http://www.nap.edu/catalog/10977.html 12 SAFETY OF GENETICALLY ENGINEERED...
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Báo cáo hóa học: "Anti-viral state segregates two molecular phenotypes of pancreatic adenocarcinoma: potential relevance for adenoviral gene therapy" pdf
Ngày tải lên :
18/06/2014, 16:20
... myxovirus-resistance A; PDAC: pancreatic ductal adenocarcinoma; TMA: tissue microarray; X-PDAC: xenografted primary pancreatic ductal adenocarcinomas Additional file 1: Sets of siRNA duplexes used for ... “anti-viral state” is a characteristic molecular phenotype of a subset of pancreatic cancers that may be the result of a specific mutational profile of cancer cells which is difficult to be understood ... Blot analysis of MxA in 11 xenografted primary pancreatic adenocarcinomas (X-PDAC) C) Example of MxA immuno positive (X-PDAC 4) and MxA immuno negative (XPDAC 6) samples D) Correlation of MxA immunohistochemistry,...
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Báo cáo sinh học: "Histone modification enhances the effectiveness of IL-13 receptor targeted immunotoxin in murine models of human pancreatic cancer" doc
Ngày tải lên :
18/06/2014, 19:20
... Expression of human and mouse IL-13Ra2 protein in pancreatic cancer cell lines and mouse organs was observed by indirect immunofluorescence-immunostaining as described previously [28,30] using anti -mouse ... is ratio of mouse IL-13Ra2/b-actin expression and multiplied by 100 for convenience C, IHC of human IL-13Ra2 in implanted pancreatic tumors after SAHA and IL-13-PE treatment D, IHC of mouse IL-13Ra2 ... expression in pancreatic cancer cell lines Eleven pancreatic cancer cell lines and three types of normal cell lines (fibroblast, umbilical vein endothelial cells and pancreatic ductal epithelial...
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báo cáo hóa học:" Histone modification enhances the effectiveness of IL-13 receptor targeted immunotoxin in murine models of human pancreatic cancer" pot
Ngày tải lên :
20/06/2014, 03:20
... Expression of human and mouse IL-13Ra2 protein in pancreatic cancer cell lines and mouse organs was observed by indirect immunofluorescence-immunostaining as described previously [28,30] using anti -mouse ... is ratio of mouse IL-13Ra2/b-actin expression and multiplied by 100 for convenience C, IHC of human IL-13Ra2 in implanted pancreatic tumors after SAHA and IL-13-PE treatment D, IHC of mouse IL-13Ra2 ... expression in pancreatic cancer cell lines Eleven pancreatic cancer cell lines and three types of normal cell lines (fibroblast, umbilical vein endothelial cells and pancreatic ductal epithelial...
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báo cáo hóa học:" Ovarian cancer mouse models: a summary of current models and their limitations" docx
Ngày tải lên :
20/06/2014, 07:20
... expression of SV40 TAg to the epithelium of the female reproductive tract by microinjection of this construct into the male pronucleus of 0.5-day old embryos to generate transgenic animals While 18 of ... alternative method of generation Genetically induced granulosa cell tumor (GCT) models Granulosa cell tumors (GCT) represent 2-5% of all OCs [53] arising from the granulosa cells of the ovary, which ... TAg immortalization of cultured human OSE cells eliminated the presence of CA-125 [69], one of the current diagnostic markers for EOC [70] To understand the complexity of OC, a mouse model representing...
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Báo cáo y học: " Identification of arthritis-related gene clusters by microarray analysis of two independent mouse models for rheumatoid arthritis" pdf
Ngày tải lên :
09/08/2014, 08:22
... three isoforms of IL-1Ra protein, which possess inhibitory activity against IL-1, are synthesized by alternative splicing of a single gene, we produced mice deficient in all three isoforms of IL1Ra ... controls The severity of arthritis was graded for each paw on a scale of to for the degree of redness and swelling: grade = normal; grade = light swelling of the joint and/or redness of the footpad; ... group of genes is involved in the pathogenesis of arthritis in both models, and we extracted commonly changed genes in both models To extract commonly changed genes, we applied the principle of...
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Báo cáo y học: " Cross-packaging of genetically distinct mouse and primate retroviral RNAs" pdf
Ngày tải lên :
12/08/2014, 23:21
... amino acid sequences of MPMV and MMTV RNaseH and integrase Because of the lack of any published sequences of MMTV integrase and RNaseH, we took the amino acid sequences of MPMV integrase [29] ... case of pDA024 and EGFP gene in the case of pSS013) will transduce the target cells resulting in Hygr colonies or EGFP positive cells suggesting the successful completion of crucial steps of the ... if packaged, the level of packaging was below the threshold level of detection), and consequently the vector RNA could not be propagated (Table 1) The lack of packaging of these non-specific RNAs...
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Báo cáo y học: " The utilization of humanized mouse models for the study of human retroviral infections" docx
Ngày tải lên :
12/08/2014, 23:22
... or NOG mouse model Rag2-/- c-/- mouse model SCID-hu Thy/Liv mouse model hu-PBL SCID mouse model NOD/SCID 2-microglobulin-/- mouse model Humanized Mouse Model History Figure of humanized mouse ... development of a variety of immunocompromised strains of mouse [69] Most of these animals are the result of adjustments made to the 1983 CB17-scid mouse model It was in this model that engraftment of ... mutation All of these mouse models have developed over time to various degrees of accuracy and efficiency of xenotransplantation of human cells/tissues as well as the development of a functioning...
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Báo cáo sinh học: "Anti-tumor effects of a human VEGFR-2-based DNA vaccine in mouse models" pps
Ngày tải lên :
14/08/2014, 19:22
... of therapeutic anti-tumor immunity Induction of therapeutic anti-tumor immunity Mice (five per group in both the CT26 and 4T1 tumor models) were treated i.v once a week for weeks with 20 μg of ... range of 300–325 nm (The preparation of liposome DNA complexes was supported by The Chemical Laboratories of the State Key Laboratory for Biotherapy) Therapeutic anti-tumor immunity and tumor models ... surviving mice Protective anti-tumor immunity and tumor models At 6–8 weeks of age, female BALB/c mice were immunized with 20 μg per injection per mouse of pORF-hFlk-1 LDC, pORF-mFlk-1 LDC, pORF-mcs LDC...
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Effects of andrographolide and 14 deoxy 11, 12 didehydroandrographolide in obstructive respiratory disease mouse models
Ngày tải lên :
09/09/2015, 10:06
... Guan Shou Ping iii TABLE OF CONTENTS DECLARATION ii ACKNOWLEDGEMENTS iii TABLE OF CONTENTS iv SUMMARY viii LIST OF TABLES x LIST OF FIGURES xi LIST OF ABBREVIATIONS xiv LIST OF PUBLICATIONS AND CONFERENCE ... Burden of Asthma 1.2.2 Pathophysiology of Asthma 1.2.2.1 Inflammatory and Structural Cells 1.2.2.2 Mediators of Asthma 22 1.2.2.3 Airway Hyperresponsiveness (AHR) 32 1.2.3 The mouse model of asthma ... system 77 Figure 3.3 Type of cells found in BAL fluid of mice 80 Figure 3.4 Type of cells found in BAL fluid of mice 81 Figure 3.5 The FinePointe™ system 86 Figure 4.1 Effects of andrographolide and...
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